公开(公告)号：US20180216253A1

公开(公告)日：2018-08-02

申请号：US15881930

申请日：2018-01-29

Applicant: Counsyl, Inc.

Inventor： Henry H. Lai ,  Clement S. Chu

IPC: C40B50/06 ,  C12Q1/6876 ,  B01J19/00 ,  C12N15/10 ,  C12Q1/6869

CPC classification number: C40B50/06 ,  B01J19/0046 ,  B01J2219/00315 ,  C12N15/1093 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q1/6876 ,  C40B40/06 ,  C40B50/14 ,  C12Q2527/125 ,  C12Q2527/137 ,  C12Q2535/122 ,  C12Q2563/159

Abstract: Disclosed are high concentration reagents for use in preparing DNA samples in low volume reactions. Such reagents include, for example, DNA end repair buffers for use in low volume DNA blunting and phosphorylating reactions, DNA adenylating buffers for use in a low volume DNA adenylating reaction, and DNA ligation buffers for use in low volume DNA adaptor ligation reactions with adaptors. Also disclosed are customized reagent plates and kits containing one or more of these low volume buffers for use in low volume DNA blunting, phosphorylating, adenylating, and ligation reactions. Methods of using the high concentration reagents (low volume buffers) and the customized reagent plates for preparing DNA sequencing libraries in low volume reactions are also disclosed.

公开(公告)号：US20180216183A1

公开(公告)日：2018-08-02

申请号：US15881921

申请日：2018-01-29

Applicant: Counsyl, Inc.

Inventor： Henry H. Lai ,  Clement S. Chu

IPC: C12Q1/6876 ,  C12Q1/6869 ,  C40B40/06

CPC classification number: C12Q1/6876 ,  C12Q1/68 ,  C12Q1/6869 ,  C12Q2527/125 ,  C12Q2527/137 ,  C12Q2527/143 ,  C12Q2527/146 ,  C12Y207/01078 ,  C40B40/06 ,  C12Q2521/101

Abstract: Disclosed are high concentration reagents for use in preparing DNA samples in low volume reactions. Such reagents include, for example, DNA end repair buffers for use in low volume DNA blunting and phosphorylating reactions, DNA adenylating buffers for use in a low volume DNA adenylating reaction, and DNA ligation buffers for use in low volume DNA adaptor ligation reactions with adaptors. Also disclosed are customized reagent plates and kits containing one or more of these low volume buffers for use in low volume DNA blunting, phosphorylating, adenylating, and ligation reactions. Methods of using the high concentration reagents (low volume buffers) and the customized reagent plates for preparing DNA sequencing libraries in low volume reactions are also disclosed.

公开(公告)号：US20180214840A1

公开(公告)日：2018-08-02

申请号：US15881942

申请日：2018-01-29

Applicant: Counsyl, Inc.

Inventor： Henry H. Lai ,  Clement S. Chu

IPC: B01J19/00 ,  B01L3/00

CPC classification number: B01J19/0046 ,  B01J19/0033 ,  B01J19/0093 ,  B01J2219/00317 ,  B01J2219/00351 ,  B01L3/50 ,  C12Q1/00 ,  C40B40/06 ,  C40B50/14

Abstract: Disclosed are high concentration reagents for use in preparing DNA samples in low volume reactions. Such reagents include, for example, DNA end repair buffers for use in low volume DNA blunting and phosphorylating reactions, DNA adenylating buffers for use in a low volume DNA adenylating reaction, and DNA ligation buffers for use in low volume DNA adaptor ligation reactions with adaptors. Also disclosed are customized reagent plates and kits containing one or more of these low volume buffers for use in low volume DNA blunting, phosphorylating, adenylating, and ligation reactions. Methods of using the high concentration reagents (low volume buffers) and the customized reagent plates for preparing DNA sequencing libraries in low volume reactions are also disclosed.

公开(公告)号：US20180346963A1

公开(公告)日：2018-12-06

申请号：US15994624

申请日：2018-05-31

Applicant: Counsyl, Inc.

Inventor： Noah C. Welker ,  Clement S. Chu

IPC: C12Q1/6806 ,  C12Q1/6869

Abstract: Methods for preparing concatenated nucleic acid molecules are provided. The methods herein include adaptors with complementary sequences for preparation of concatenated nucleic acid molecules, and methods of sequencing such nucleic acids.

公开(公告)号：US20180216176A1

公开(公告)日：2018-08-02

申请号：US15873687

申请日：2018-01-17

Applicant: Counsyl, Inc.

Inventor： Clement S. Chu ,  Noah C. Welker ,  Henry H. Lai

IPC: C12Q1/6869 ,  C12N15/10 ,  C12N9/00 ,  C12Q1/686

CPC classification number: C12Q1/6869 ,  C12N9/93 ,  C12N15/1031 ,  C12Q1/6809 ,  C12Q1/686 ,  C12Q2600/156 ,  C12Q2525/191 ,  C12Q2531/113 ,  C12Q2535/101 ,  C12Q2535/122

Abstract: High-fidelity, high-throughput nucleic acid sequencing enables healthcare practitioners and patients to gain insight into genetic variants and potential health risks. However, previous methods of nucleic acid sequencing often introduce sequencing errors (for example, mutations that arise during the preparation of a nucleic acid library, during amplification, or sequencing). Provided herein are methods and compositions for sequencing nucleic acids. Further provided are methods of identifying an error in a nucleic acid sequence.

公开(公告)号：US20180216103A1

公开(公告)日：2018-08-02

申请号：US15873667

申请日：2018-01-17

Applicant: Counsyl, Inc.

Inventor： Henry H. Lai ,  Clement S. Chu

IPC: C12N15/10 ,  C12Q1/686 ,  C12Q1/6806

CPC classification number: C12N15/1093 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q2600/112 ,  C12Q2600/124 ,  C12Q2521/319 ,  C12Q2525/125 ,  C12Q2563/179 ,  C12Q2565/519

Abstract: High-fidelity, high-throughput nucleic acid sequencing enables healthcare practitioners and patients to gain insight into genetic variants and potential health risks. However, previous methods of nucleic acid sequencing often introduces sequencing errors (for example, mutations that arise during the preparation of a nucleic acid library, during amplification, or sequencing). Provided herein are methods and compositions for sequencing nucleic acids. Further provided are methods of identifying an error in a nucleic acid sequence.