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公开(公告)号：US06399353B1
公开(公告)日：2002-06-04
申请号：US08208807
申请日：1994-03-10
IPC分类号： C12N700
CPC分类号： C12N7/00 , A61K39/00 , C12N5/0629 , C12N2501/70 , C12N2510/02 , C12N2710/20051 , G01N33/5008 , G01N33/56983 , G01N2333/025 , G01N2500/10
摘要： The present invention provides a process of biosynthesizing papillomavirus by inducing complete differentiation of an epithelial cell that contains papillomavirus DNA. Complete differentiation is induced by exposing epithelial cells to a protein kinase C inducer. Assays for screening agents that modify papillomavirus biosynthesis, determining the papillomavirus infectivity of epithelial cells, detecting the presence of anti-papillomavirus antibodies and vaccinating against papillomavirus infection are also provided.
摘要翻译： 本发明提供了通过诱导含有乳头瘤病毒DNA的上皮细胞的完全分化来生物合成乳头瘤病毒的方法。 通过将上皮细胞暴露于蛋白激酶C诱导物诱导完全分化。 还提供了修饰乳头瘤病毒生物合成的筛选试剂的测定，确定上皮细胞的乳头瘤病毒感染性，检测抗乳头状瘤病毒抗体的存在和针对乳头瘤病毒感染的疫苗。
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公开(公告)号：US20120213736A1
公开(公告)日：2012-08-23
申请号：US13324484
申请日：2011-12-13
申请人： Craig M. Meyers , Samina Alam
发明人： Craig M. Meyers , Samina Alam
IPC分类号： A61K38/16 , A61K35/76 , A61P35/00 , A61K31/711
CPC分类号： A61K35/768 , A61K38/00 , C07K14/005 , C12N7/00 , C12N2750/14122 , C12N2750/14132 , C12N2750/14133
摘要： According to the invention, parvoviruses such as the adeno-associated virus Type 2 (AAV2) are found to be oncolytic, selectively mediating apoptosis in cancer cells and their precursors, while leaving healthy cells intact. The invention also includes a method of killing cancer and other neoplastic and preneoplastic cells by administrating to said cells the AAV2 proteins Rep78 or Rep 68, expression constucts encoding the same, or pharmaceutical compositions comprising the same.
摘要翻译： 根据本发明，发现细小病毒如腺相关病毒2型（AAV2）是溶瘤的，选择性介导癌细胞及其前体中的细胞凋亡，同时保持健康细胞完整。 本发明还包括通过向所述细胞施用AAV2蛋白Rep78或Rep 68，编码其的表达构架或包含其的药物组合物来杀死癌症和其它肿瘤和肿瘤前细胞的方法。
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公开(公告)号：US08080240B2
公开(公告)日：2011-12-20
申请号：US11577782
申请日：2005-10-21
申请人： Craig M. Meyers , Samina Alam
发明人： Craig M. Meyers , Samina Alam
CPC分类号： C12N7/00 , A61K35/768 , C12N2750/14132
摘要： According to the invention, parvoviruses such as the adeno-associated virus Type 2 (AAV2) are found to be oncolytic, selectively mediating apoptosis in cancer cells and their precursers, while leaving healthy cells intact. The invention thus comprises a method of killing cancer and other neoplastic and preneoplastic cells by administration of AAV2 virus, viral particles, products or replication incompetent vectors derived there from to said cells, and pharmaceutical compositions comprising the same.
摘要翻译： 根据本发明，发现细小病毒如腺相关病毒2型（AAV2）是溶瘤的，选择性介导癌细胞及其前体中的细胞凋亡，同时保持健康细胞完整。 因此，本发明包括通过施用从所述细胞获得的AAV2病毒，病毒颗粒，产物或复制无能载体以及包含其的药物组合物来杀死癌症和其它肿瘤和肿瘤前细胞的方法。
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4.发明授权
公开(公告)号：US5994115A
公开(公告)日：1999-11-30
申请号：US898636
申请日：1997-07-22
申请人： Craig M. Meyers
发明人： Craig M. Meyers
CPC分类号： C12N7/00 , A61K39/12 , C12N2710/20034 , G01N2333/025
摘要： Artificial systems for the production of infectious human papillomavirus allow development of diagnostic and therapeutic strategies based on replication and infectivity studies for diseases caused by these viruses. The artificial introduction of papillomavirus genomic DNA into an epithelial cell line. In particular, HPV type 18 genomic DNA is transfected into primary keratinocytes. Transfection is followed by clonal expansion of the transfected cells, and induction of epithelial stratification and differentiation in organotypic cultures resulting in the synthesis of virions. Virus particles synthesized were approximately 50 nanometers in diameter as observed by electron microscopy. In particular HPV18 virions produced by the system and purified by an isopycnic gradient were capable of infecting keratinocytes.
摘要翻译： 用于生产感染性人乳头瘤病毒的人造系统允许基于由这些病毒引起的疾病的复制和感染性研究开发诊断和治疗策略。 将乳头瘤病毒基因组DNA人工引入上皮细胞系。 特别地，将HPV 18型基因组DNA转染入原代角质形成细胞。 转染之后是转染的细胞的克隆扩增，以及在器官型培养物中的上皮分层和分化的诱导，导致病毒粒子的合成。 合成的病毒颗粒直径约为50纳米，通过电子显微镜观察。 特别是由系统产生并通过等摩尔梯度纯化的HPV18病毒体能够感染角化细胞。
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公开(公告)号：US08980247B2
公开(公告)日：2015-03-17
申请号：US13324484
申请日：2011-12-13
申请人： Craig M. Meyers , Samina Alam
发明人： Craig M. Meyers , Samina Alam
IPC分类号： C12N15/35 , C12N7/00 , C07K14/005 , C12N15/864 , A61K38/00
CPC分类号： A61K35/768 , A61K38/00 , C07K14/005 , C12N7/00 , C12N2750/14122 , C12N2750/14132 , C12N2750/14133
摘要： According to the invention, parvoviruses such as the adeno-associated virus Type 2 (AAV2) are found to be oncolytic, selectively mediating apoptosis in cancer cells and their precursors, while leaving healthy cells intact. The invention also includes a method of killing cancer and other neoplastic and preneoplastic cells by administrating to said cells the AAV2 proteins Rep78 or Rep 68, expression constucts encoding the same, or pharmaceutical compositions comprising the same.
摘要翻译： 根据本发明，发现细小病毒如腺相关病毒2型（AAV2）是溶瘤的，选择性介导癌细胞及其前体中的细胞凋亡，同时保持健康细胞完整。 本发明还包括通过向所述细胞施用AAV2蛋白Rep78或Rep 68，编码其的表达构架或包含其的药物组合物来杀死癌症和其它肿瘤和肿瘤前细胞的方法。
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公开(公告)号：US20090117081A1
公开(公告)日：2009-05-07
申请号：US11577782
申请日：2005-10-21
申请人： Craig M. Meyers , Samina Alam
发明人： Craig M. Meyers , Samina Alam
CPC分类号： C12N7/00 , A61K35/768 , C12N2750/14132
摘要： According to the invention, parvoviruses such as the adeno-associated virus Type 2 (AAV2) are found to be oncolytic, selectively mediating apoptosis in cancer cells and their precursers, while leaving healthy cells intact. The invention thus comprises a method of killing cancer and other neoplastic and preneoplastic cells by administration of AAV2 virus, viral particles, products or replication incompetent vectors derived there from to said cells, and pharmaceutical compositions comprising the same.
摘要翻译： 根据本发明，发现细小病毒如腺相关病毒2型（AAV2）是溶瘤的，选择性介导癌细胞及其前体中的细胞凋亡，同时保持健康细胞完整。 因此，本发明包括通过施用从所述细胞获得的AAV2病毒，病毒颗粒，产物或复制无能载体以及包含其的药物组合物来杀死癌症和其它肿瘤和肿瘤前细胞的方法。
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公开(公告)号：US06841157B2
公开(公告)日：2005-01-11
申请号：US10332553
申请日：2001-07-03
IPC分类号： C12N15/09 , C07K14/025 , C12N5/10 , C12N7/00 , C12N7/01 , C12N7/02 , C12N15/37 , C12N15/86 , C12Q1/68 , C12Q1/70 , A61K39/12
CPC分类号： C07K14/005 , C12N7/00 , C12N15/86 , C12N2710/20021 , C12N2710/20022 , C12N2710/20043 , C12N2710/20044 , C12N2800/108
摘要： This invention relates to infectious chimeric papillomaviruses, and especially those where the early genes are from human papillomavirus (HPV) 18, and the late genes are from another HPV. Also presented are methods of culturing the virus in raft cell cultures, and to assays utilizing these chimeric viruses.
摘要翻译： 本发明涉及感染性嵌合乳头瘤病毒，特别是早期基因来自人乳头状瘤病毒（HPV）18，晚期基因来自另一HPV的那些。 还提出了在筏细胞培养物中培养病毒的方法以及利用这些嵌合病毒的测定法。
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8.发明授权Methods for detecting, titering, and determining susceptibility to papillomavirus 有权用于检测，滴定和确定对乳头瘤病毒敏感性的方法
公开(公告)号：US6110663A
公开(公告)日：2000-08-29
申请号：US190433
申请日：1998-11-12
申请人： Craig M. Meyers , Michelle A. Ozbun
发明人： Craig M. Meyers , Michelle A. Ozbun
IPC分类号： C07K14/025 , G01N33/569 , G01N33/68 , C12G1/70 , C12N15/00 , C12P21/04 , G01N33/567
CPC分类号： C07K14/005 , G01N33/56983 , G01N33/6875 , C12N2710/20022 , G01N2333/025
摘要： Expression vectors that include reporter genes and an operable regulatory region containing a promoter and E2 binding sites of papillomavirus (PV), are used to detect and/or titer papillomavirus by quantitative or qualitative methods.
摘要翻译： 包括报告基因和含有启动子和乳头瘤病毒（PV）的E2结合位点的可操作调节区域的表达载体用于通过定量或定性方法检测和/或滴度乳头瘤病毒。
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