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公开(公告)号：US10988750B2
公开(公告)日：2021-04-27
申请号：US16276558
申请日：2019-02-14
Applicant: Danisco US Inc. , The Goodyear Tire & Rubber Company
Inventor： Zachary Q. Beck , Jeffrey W. Munos , Derek H. Wells , Jian Yao
Abstract: This present invention relates to cultured recombinant cells comprising a heterologous phosphoketolase (PKL) polypeptide that are capable of increased production of acetyl coenzyme A-derived metabolites, as well as methods for producing and using the same. In some embodiments, the recombinant cells further comprise one or more mevalonate (MVA) pathway polypeptides for the production of isoprenoid precursors, isoprene and isoprenoids.
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2.发明授权公开(公告)号：US09493791B2
公开(公告)日：2016-11-15
申请号：US14625487
申请日：2015-02-18
Applicant: Danisco US Inc. , The Goodyear Tire & Rubber Company
Inventor： Zachary Q. Beck , Andrew C. Eliot , Caroline M. Peres , Dmitrii V. Vaviline
CPC classification number: C12P5/007 , C12N1/12 , C12N1/14 , C12N1/20 , C12N9/1085 , C12N9/88 , C12N15/70 , C12P7/42 , C12Y203/01008 , C12Y205/01 , C12Y401/02009 , C12Y402/03027 , C12Y503/03002 , Y02P20/52
Abstract: The invention provides for methods for the production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids in cells via the heterologous expression of phosphoketolase enzymes.
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3.发明授权Three-dimensional structure of isoprene synthase and its use thereof for generating variants 有权异戊二烯合酶的三维结构及其用于产生变体的用途公开(公告)号：US09175313B2
公开(公告)日：2015-11-03
申请号：US13888265
申请日：2013-05-06
Applicant: Danisco US Inc. , The Goodyear Tire & Rubber Company
Inventor： Zachary Q. Beck , Richard R. Bott , Jeffrey V. Miller , Christopher L. Rife , Derek H. Wells
CPC classification number: C12P5/007 , C07K2299/00 , C12N9/88 , C12Y402/03027
Abstract: The present invention provides a three-dimensional structures of P. tremuloides isoprene synthase and P. alba isoprene synthase. The invention also provides methods of using the three-dimensional structure to design isoprene synthases with improved activity for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.
Abstract translation: 本发明提供了三叶虫异戊二烯合酶和异戊二烯异戊二烯合酶的三维结构。 本发明还提供使用三维结构设计异戊二烯合酶的方法，所述异戊二烯合酶具有改进的微生物宿主细胞中异戊二烯生产增加的活性。 本发明的生物合成生产的异戊二烯可用于制造橡胶和弹性体。
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公开(公告)号：US08889387B2
公开(公告)日：2014-11-18
申请号：US13888248
申请日：2013-05-06
Applicant: Danisco US Inc. , The Goodyear Tire & Rubber Company
Inventor： Zachary Q. Beck , Marguerite A. Cervin , Alex T. Nielsen , Caroline M. Peres
IPC: C12P7/42 , C12P5/02 , C12N9/00 , C12N1/20 , C12N15/00 , C07H21/04 , C12N15/70 , C12P5/00 , C12N15/52 , C12N15/63
CPC classification number: C12N15/52 , C12N15/635 , C12N15/70 , C12P5/007 , C12P7/42
Abstract: Provided herein are improved compositions and methods for the increased production of isoprene. Also provided herein are improved compositions and methods for the increased production of heterologous polypeptides capable of biological activity.
Abstract translation: 本文提供用于增加异戊二烯生产的改进的组合物和方法。 本文还提供了用于增加能够生物活性的异源多肽的生产的改进的组合物和方法。
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公开(公告)号：US20180002683A1
公开(公告)日：2018-01-04
申请号：US15536817
申请日：2015-12-18
Applicant: DANISCO US INC.
Inventor： Zachary Q. Beck , Meredith K. Fujdala , Henrik Hansson , Thijs Kaper , Slavko Kralj , Amy D. Liu , Nils Egil Mikkelsen , Mats Sandgren
CPC classification number: C12N9/248 , C12N9/2445 , C12P19/02 , C12P19/14 , C12Y302/01021 , C12Y302/01037
Abstract: Provided are certain glycosyl hydrolase family 3 (GH3) beta-xylosidases engineered to acquire beta-glucosidase activities. Provided also are compositions comprising such multi-functional GH3 enzymes and methods of use or industrial applications thereof.
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Patent Agency Ranking
公开(公告)号：US09752162B2
公开(公告)日：2017-09-05
申请号：US14514962
申请日：2014-10-15
Applicant: Danisco US Inc. , The Goodyear Tire & Rubber Company
Inventor： Zachary Q. Beck , Michael C. Miller , Caroline M. Peres , Yuliya A. Primak , Jeff P. Pucci , Derek H. Wells
CPC classification number: C12P5/007 , C12N9/0006 , C12N9/1025 , C12N9/1029 , C12N15/70 , C12N15/81 , C12P5/002 , C12P7/02 , C12P7/04 , C12P7/42 , C12Y101/01034 , C12Y203/01016 , C12Y203/0301 , Y02E50/343
Abstract: The invention features compositions and methods for the increased production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids in microorganisms via the heterologous expression of the mvaE and mvaS genes from the organisms Listeria grayi DSM 20601, Enterococcus faecium, Enterococcus gallinarum EG2, and Enterococcus casseliflavus.
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公开(公告)号：US09464301B2
公开(公告)日：2016-10-11
申请号：US14323596
申请日：2014-07-03
Applicant: Danisco US Inc. , The Goodyear Tire & Rubber Company
Inventor： Zachary Q. Beck , Anthony R. Calabria , Michael C. Miller , Alex T. Nielsen , Dmitrii V. Vaviline
CPC classification number: C12P5/007 , C12N9/1205 , C12N9/88 , C12P9/00 , C12Y207/01036 , C12Y402/03027 , Y02P20/52
Abstract: The invention features methods for producing isoprene from cultured cells using a feedback-resistant mevalonate kinase polypeptide, such as an archaeal mevalonate kinase polypeptide. The resulting isoprene compositions may have increased yields and/or purity of isoprene.
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8.发明授权公开(公告)号：US10975394B2
公开(公告)日：2021-04-13
申请号：US16200485
申请日：2018-11-26
Applicant: Danisco US Inc. , The Goodyear Tire & Rubber Company
Inventor： Zachary Q. Beck , Michael C. Miller , Caroline M. Peres , Yuliya A. Primak , Jeff P. Pucci , Derek H. Wells
IPC: C12P5/00 , C12N9/04 , C12N9/02 , C12N9/10 , C12N9/12 , C12N9/18 , C12N9/88 , C12P7/02 , C12P7/04 , C12P7/42 , C12N15/70 , C12P5/02
Abstract: The invention features compositions and methods for the increased production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids in microorganisms by engineering a microorganism for increased carbon flux towards mevalonate production in the following enzymatic pathways: (a) citrate synthase, (b) phosphotransacetylase, (c) acetate kinase, (d) lactate dehydrogenase, (e) malic enzyme, and (f) pyruvate dehydrogenase such that one of more of the enzyme activity is modulated. In addition, production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids can be further enhanced by the heterologous expression of the mvaE and mvaS genes (such as, but not limited to, mvaE and mvaS genes from the organisms Listeria grayi DSM 20601, Enterococcus faecium, Enterococcus gallinarum EG2, and Enterococcus casseliflavus).
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公开(公告)号：US10364443B2
公开(公告)日：2019-07-30
申请号：US15694193
申请日：2017-09-01
Applicant: Danisco US Inc. , The Goodyear Tire & Rubber Company
Inventor： Zachary Q. Beck , Michael C. Miller , Caroline M. Peres , Yuliya A. Primak , Jeff P. Pucci , Derek H. Wells
Abstract: The invention features compositions and methods for the increased production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids in microorganisms via the heterologous expression of the mvaE and mvaS genes from the organisms Listeria grayi DSM 20601, Enterococcus faecium, Enterococcus gallinarum EG2, and Enterococcus casseliflavus.
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10.发明授权公开(公告)号：US08815548B2
公开(公告)日：2014-08-26
申请号：US13730661
申请日：2012-12-28
Applicant: Danisco US Inc. , The Goodyear Tire & Rubber Company
Inventor： Zachary Q. Beck , Anthony R. Calabria , Michael C. Miller , Alex T. Nielsen , Dmitrii V. Vaviline
IPC: C12P9/00
CPC classification number: C12P5/007 , C12N9/1205 , C12N9/88 , C12P9/00 , C12Y207/01036 , C12Y402/03027 , Y02P20/52
Abstract: The invention features methods for producing isoprene from cultured cells using a feedback-resistant mevalonate kinase polypeptide, such as an archaeal mevalonate kinase polypeptide. The resulting isoprene compositions may have increased yields and/or purity of isoprene.
Abstract translation: 本发明的特征在于使用反馈抗性甲羟戊酸激酶多肽（例如古细菌甲羟戊酸激酶多肽）从培养细胞产生异戊二烯的方法。 得到的异戊二烯组合物可以具有增加的异戊二烯的产率和/或纯度。
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