公开(公告)号：US20200234936A1

公开(公告)日：2020-07-23

申请号：US16639161

申请日：2018-08-31

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Douglas Arnold Simmons ,  Nic G. Bloomfield

IPC: H01J49/00 ,  H01J49/06 ,  H01J49/42

Abstract: Dynamic skimmer pulsing and dynamic equilibration times are used for MS and MS/MS scans. A target percentage transmission of the ion beam is calculated based on a previous percentage transmission and a previous TIC or a previous highest intensity of a previous cycle time. An equilibration time is calculated based on the current percentage transmission and the target percentage transmission. A skimmer of a tandem mass spectrometer is controlled to attenuate the ion beam to the target percentage transmission to prevent saturation of a detector of the tandem mass spectrometer and to increase the dynamic range of the tandem mass spectrometer. The tandem mass spectrometer is controlled to perform an MS scan or an MS/MS scan after the calculated equilibration time to reduce the cycle time.

公开(公告)号：US20170250065A1

公开(公告)日：2017-08-31

申请号：US15513267

申请日：2015-09-21

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Nic G. Bloomfield

IPC: H01J49/40 ,  H01J49/00 ,  H01J49/04 ,  H01J49/28 ,  G01N27/62 ,  H01J49/26

CPC classification number: H01J49/40 ,  G01N27/62 ,  H01J49/0036 ,  H01J49/04 ,  H01J49/26

Abstract: Sample molecules are ionized producing a beam of ions using an ion source. A plurality of ion extractions are performed on the beam of ions using a TOF mass spectrometer. Electrical detections from each extraction are measured using an ADC, producing a mass sub-spectrum for each extraction. An ion m/z from the plurality of mass sub-spectra is selected. For each mass sub-spectrum, the amplitude and m/z of an ion within a m/z tolerance of the ion m/z is assigned to the corresponding amplitude band of a plurality of predetermined amplitude bands, producing a plurality of amplitude and m/z values for the each amplitude band. For each amplitude band of the plurality of predetermined amplitude bands, the plurality of amplitude and m/z values are combined into a peak, resulting in a plurality of peaks corresponding to the plurality of predetermined amplitude bands.

公开(公告)号：US20160148791A1

公开(公告)日：2016-05-26

申请号：US15008385

申请日：2016-01-27

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Nic G. Bloomfield ,  Alexandre V. Loboda

IPC: H01J49/00 ,  H01J49/40

CPC classification number: H01J49/0009 ,  H01J49/0036 ,  H01J49/025 ,  H01J49/40

Abstract: Systems and methods are provided for correcting uniform detector saturation of a mass analyzer using a calibration curve. In one method, a measured spectrum is received from a mass analyzer that includes a detector and an analog-to-digital converter (ADC) detector subsystem and that analyzes a beam of ions produced by an ion source that ionizes molecules of a sample using a processor. A total ion value of the measured spectrum is calculated by summing intensities of ions in the measured spectrum using the processor. A correction factor is determined by comparing the total ion value to a stored calibration curve that provides correction factors as a function of total ion values using the processor. Intensities of the measured spectrum are multiplied by the determined correction factor producing a corrected measured spectrum using the processor.

Abstract translation: 提供了使用校准曲线校正质量分析器的均匀检测器饱和度的系统和方法。 在一种方法中，从包括检测器和模拟 - 数字转换器（ADC）检测器子系统的质量分析器接收测量的光谱，并且分析由离子源产生的离子束，其使用离子源离子化样品的分子 处理器。 通过使用处理器对所测量的光谱中的离子的强度求和来计算测量光谱的总离子值。 通过将总离子值与存储的校准曲线进行比较来确定校正因子，该校准曲线使用处理器提供作为总离子值的函数的校正因子。 测量光谱的强度乘以确定的校正因子，使用处理器产生校正的测量光谱。

公开(公告)号：US20240331991A1

公开(公告)日：2024-10-03

申请号：US18739836

申请日：2024-06-11

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Nic G. Bloomfield ,  Gordana Ivosev

IPC: H01J49/00 ,  G06F17/16 ,  G06F17/17

CPC classification number: H01J49/0036 ,  G06F17/16 ,  G06F17/17 ,  H01J49/0045

Abstract: A precursor ion transmission window is moved in overlapping steps across a precursor ion mass range. The precursor ions transmitted at each overlapping step by the mass filter are fragmented or transmitted. Intensities or counts are detected for each of the one or more resulting product ions or precursor ions for each overlapping window that form mass spectrum data for each overlapping window. Each unique product ion detected is encoded in real-time during data acquisition. This encoding includes sums of counts or intensities of each unique ion detected the overlapping windows and positions of the windows associated with each sum. The encoding for each unique ion is stored in a memory device rather than the mass spectral data. A deblurring algorithm or numerical method is used to determine a precursor ion of each unique ion from the encoded data.

公开(公告)号：US11953478B2

公开(公告)日：2024-04-09

申请号：US17593349

申请日：2020-03-10

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Nic G. Bloomfield ,  Lyle Lorrence Burton ,  John Lawrence Campbell ,  David M. Cox ,  Eva Duchoslav ,  Adam San-Ho Lau

IPC: H01J49/00 ,  G01N30/72 ,  G01N30/86 ,  H01J49/04 ,  H01J49/16 ,  H01J49/42 ,  H01J49/44

CPC classification number: G01N30/8631 ,  G01N30/7206 ,  G01N30/7233 ,  H01J49/0031 ,  H01J49/0418 ,  H01J49/167 ,  H01J49/4225 ,  H01J49/446

Abstract: Each of one or more unknown compounds are separated from a sample over a separation time period. Separated compounds are ionized, producing one or more compound precursor ions for each of the unknown compounds and a plurality of background precursor ions. A precursor ion mass spectrum is measured for the combined compound and background precursor ions at each time step of a plurality of time steps spread across the separation time period, producing a plurality of precursor ion mass spectra. One or more background precursor ions are selected from the plurality of precursor ion mass spectra that have a resolving power in a range below a threshold expected resolving power. A separation time is detected for an unknown compound when a decrease in an intensity measurement of the selected background precursor ions over a time period exceeds a threshold decrease in intensity with respect to time.

公开(公告)号：US20220199390A1

公开(公告)日：2022-06-23

申请号：US17310959

申请日：2020-04-15

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： David Michael Cox ,  Nic G. Bloomfield

IPC: H01J49/40 ,  H01J49/02 ,  H01J49/00

Abstract: The resolution of a TOF mass analyzer is maintained despite a loss of resolution in one or more channels of a multichannel ion detection system by selecting the highest resolution channels for qualitative analysis. Ion packets that impact a multichannel detector are converted into multiplied electrons and emitted from two or more segmented electrodes that correspond to impacts in different regions across a length of the detector. The electrons received by each electrode of the two or more segmented electrodes for each ion packet are converted into digital values in a channel of a multichannel digitizer, producing digital values for at least two or more channels Qualitative information about the ion packets is calculated using digital values of a predetermined subset of one or more channels of the at least two or more channels known to provide the highest resolution.

公开(公告)号：US10784098B2

公开(公告)日：2020-09-22

申请号：US16490400

申请日：2018-03-01

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Igor Chernushevich ,  Nic G. Bloomfield

IPC: H01J49/40 ,  H01J49/02 ,  H01J49/00 ,  H01J49/06

Abstract: Two-channel electrical and photo-electrical TOF ion detection systems are provided. These systems maintain the resolution and dynamic range advantages of four-channel systems but at a lower cost. Electrodes or light pipes are configured to direct electrons or photons produced by ion impacts into two separate channels. The first channel receives electrons or photons resulting from the inner or central part of the rectangular pattern of each ion impact. The second channel receives electrons or photons resulting from the two outer ends of the rectangular pattern of each ion impact. In a two-channel digitizer, the first channel and the second channel are independently calibrated to align the first digital value and the second digital value in time and account for the convex shape of the ion impacts of each ion packet and/or the curvature of a microchannel plate.

公开(公告)号：US09472387B2

公开(公告)日：2016-10-18

申请号：US15089529

申请日：2016-04-02

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Nic G. Bloomfield ,  Frank Londry

IPC: H01J49/00 ,  H01J49/06

CPC classification number: H01J49/0036 ,  H01J49/0027 ,  H01J49/004 ,  H01J49/061 ,  H01J49/40 ,  H01J49/4215 ,  H01J49/427

Abstract: Ions are separated from a sample over time and filtered. The precursor ions produced at each step are fragmented. Resulting product ions are analyzed using a mass analyzer, producing a product ion spectrum for each step of the transmission window and a plurality of product ion spectra for the mass range for the each scan. The plurality of product ion spectra are received, producing a plurality of multi-scan product ion spectra. At least one product ion is selected from the plurality of multi-scan product ion spectra that is present at least two or more times in product ion spectra from each of two or more scans. A known separation profile of a precursor ion is fit to intensities from the at least one product ion in the plurality of multi-scan product ion spectra to reconstruct a separation profile of a precursor ion of the at least one product ion.

Abstract translation: 离子与样品随时间分离并过滤。 在每个步骤产生的前体离子是碎裂的。 使用质量分析仪分析所得产物离子，产生用于传输窗口的每个步骤的产物离子谱和用于每次扫描的质量范围的多个产物离子光谱。 接收多个产物离子光谱，产生多个多扫描产物离子光谱。 从多个多扫描产物离子光谱中选择至少一个产物离子，所述离子谱在两次或更多次扫描中的每一次的产物离子谱中至少存在两次或更多次。 前体离子的已知分离曲线适合于多个多扫描产物离子光谱中的至少一种产物离子的强度，以重建至少一种产物离子的前体离子的分离曲线。

公开(公告)号：US20240177982A1

公开(公告)日：2024-05-30

申请号：US18551706

申请日：2022-03-14

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Gordana Ivosev ,  Stephen A. Tate ,  Yang Kang ,  Nic G. Bloomfield

IPC: H01J49/00

CPC classification number: H01J49/0072 ,  H01J49/0036

Abstract: An uncertainty weighted average of the equalized amounts of two or more quantifier ions is calculated from a quantitation experiment itself. n known i ions of a compound are mass analyzed over time in each of m different samples, producing n XIC peaks for each of the m samples. A reference ion j is selected that is a j ion of the n i ions or a hypothetical ion j. A ratio r(j,i) of a peak area of the j ion to a peak area of each ion of the n i ions is calculated for each of the m samples, producing m r(j,i) ratios for each of the n i ions. An expected ratio rq(j,i) is calculated for each ion of the n i ions from the m r(j,i) ratios for each of the n i ions. For each sample, the uncertainty weighted average is calculated using rq(j,i).

公开(公告)号：US20220189754A1

公开(公告)日：2022-06-16

申请号：US17593142

申请日：2020-05-29

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Nic G. Bloomfield ,  Gordana Ivosev

IPC: H01J49/00 ,  G06F17/16 ,  G06F17/17

Abstract: A precursor ion transmission window is moved in overlapping steps across a precursor ion mass range. The precursor ions transmitted at each overlapping step by the mass filter are fragmented or transmitted. Intensities or counts are detected for each of the one or more resulting product ions or precursor ions for each overlapping window that form mass spectrum data for each overlapping window. Each unique product ion detected is encoded in real-time during data acquisition. This encoding includes sums of counts or intensities of each unique ion detected the overlapping windows and positions of the windows associated with each sum. The encoding for each unique ion is stored in a memory device rather than the mass spectral data. A deblurring algorithm or numerical method is used to determine a precursor ion of each unique ion from the encoded data.