公开(公告)号：US20140127825A1

公开(公告)日：2014-05-08

申请号：US14104117

申请日：2013-12-12

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Subhakar Dey ,  Sasi Pillal ,  Brian L. Williamson ,  Subhasish Purkayastha

IPC: G01N33/82

CPC classification number: G01N33/82 ,  Y10T436/20 ,  Y10T436/200833 ,  Y10T436/203332 ,  Y10T436/24 ,  Y10T436/25 ,  Y10T436/25375 ,  Y10T436/255 ,  Y10T436/25875

Abstract: Quantification of vitamin D2, vitamin D3, and the monohydroxy and diihydroxy metabolites of vitamin D2 and vitamin D3, can comprise labeling analytes with mass spectrometry (MS) tagging reagents and performing LC-MSMS analysis of the labeled analytes. The labeled analytes can include a labeled standard and can have distinct retention times on a reversed phase column, as well as distinct masses. Under high energy collisions, reporter groups can be generated. The intensity or the peak area detected for each reporter group can be used for quantitation. In some embodiments, a one-step tagging reagent is used that is a dienophile-containing, labeled m Diels Alder reagent.

Abstract translation: 维生素D 2，维生素D3和维生素D 2和维生素D3的单羟基和二羟基代谢物的定量可以包括用质谱法（MS）标记试剂标记分析物并对标记的分析物进行LC-MSMS分析。 标记的分析物可以包括标记的标准物，并且可以在反相柱上具有不同的保留时间，以及不同的质量。 在高能量碰撞下，可以产生记者组。 每个报道组检测到的强度或峰面积可用于定量。 在一些实施方案中，使用一种含有亲二烯体的标记的狄尔斯·阿德尔试剂的一步标签试剂。

公开(公告)号：US20140004616A1

公开(公告)日：2014-01-02

申请号：US13897628

申请日：2013-05-20

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Subhakar Dey ,  Sasi Pillal ,  Brian L. Williamson ,  Subhasish Purkayastha ,  Michal Weinstock

IPC: G01N27/62

CPC classification number: G01N30/72 ,  G01N27/62 ,  G01N33/64 ,  G01N33/743 ,  G01N2030/045 ,  G01N2030/884 ,  H01J49/00 ,  H01J49/0045 ,  H01J49/164 ,  Y10T436/147777 ,  Y10T436/200833

Abstract: Labeling reagents, sets of labeling reagents, and labeling techniques are provided for the relative quantitation, absolute quantitation, or both, of ketone or aldehyde compounds including, but not limited to, analytes comprising steroids or ketosteroids. The analytes can be medical or pharmaceutical compounds in biological samples. Methods for labeling, analyzing, and quantifying ketone or aldehyde compounds are also disclosed as are methods that also use mass spectrometry.

Abstract translation: 提供了标记试剂，一组标记试剂和标记技术用于酮或醛化合物的相对定量，绝对定量或两者，包括但不限于包含类固醇或酮类固醇的分析物。 分析物可以是生物样品中的医药或药物化合物。 还公开了用于标记，分析和定量酮或醛化合物的方法，也是也使用质谱法的方法。

公开(公告)号：US09500622B2

公开(公告)日：2016-11-22

申请号：US13897628

申请日：2013-05-20

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Subhakar Dey ,  Sasi Pillal ,  Brian L. Williamson ,  Subhasish Purkayastha ,  Michal Weinstock

IPC: G01N24/00 ,  G01N27/62 ,  G01N30/72 ,  G01N33/64 ,  G01N33/74 ,  G01N30/04 ,  G01N30/88 ,  H01J49/00

CPC classification number: G01N30/72 ,  G01N27/62 ,  G01N33/64 ,  G01N33/743 ,  G01N2030/045 ,  G01N2030/884 ,  H01J49/00 ,  H01J49/0045 ,  H01J49/164 ,  Y10T436/147777 ,  Y10T436/200833

Abstract: Labeling reagents, sets of labeling reagents, and labeling techniques are provided for the relative quantitation, absolute quantitation, or both, of ketone or aldehyde compounds including, but not limited to, analytes comprising steroids or ketosteroids. The analytes can be medical or pharmaceutical compounds in biological samples. Methods for labeling, analyzing, and quantifying ketone or aldehyde compounds are also disclosed as are methods that also use mass spectrometry.

Abstract translation: 提供了标记试剂，一组标记试剂和标记技术用于酮或醛化合物的相对定量，绝对定量或两者，包括但不限于包含类固醇或酮类固醇的分析物。 分析物可以是生物样品中的医药或药物化合物。 还公开了用于标记，分析和定量酮或醛化合物的方法，也是也使用质谱法的方法。

公开(公告)号：US09188596B2

公开(公告)日：2015-11-17

申请号：US14104117

申请日：2013-12-12

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Subhakar Dey ,  Sasi Pillal ,  Brian L. Williamson ,  Subhasish Purkayastha

IPC: G01N33/82 ,  G01N30/72

CPC classification number: G01N33/82 ,  Y10T436/20 ,  Y10T436/200833 ,  Y10T436/203332 ,  Y10T436/24 ,  Y10T436/25 ,  Y10T436/25375 ,  Y10T436/255 ,  Y10T436/25875

Abstract: Quantification of vitamin D2, vitamin D3, and the monohydroxy and diihydroxy metabolites of vitamin D2 and vitamin D3, can comprise labeling analytes with mass spectrometry (MS) tagging reagents and performing LC-MSMS analysis of the labeled analytes. The labeled analytes can include a labeled standard and can have distinct retention times on a reversed phase column, as well as distinct masses. Under high energy collisions, reporter groups can be generated. The intensity or the peak area detected for each reporter group can be used for quantitation. In some embodiments, a one-step tagging reagent is used that is a dienophile-containing, labeled m Diels Alder reagent.

Abstract translation: 维生素D 2，维生素D3和维生素D 2和维生素D3的单羟基和二羟基代谢物的定量可以包括用质谱法（MS）标记试剂标记分析物并对标记的分析物进行LC-MSMS分析。 标记的分析物可以包括标记的标准物，并且可以在反相柱上具有不同的保留时间，以及不同的质量。 在高能量碰撞下，可以产生记者组。 每个报道组检测到的强度或峰面积可用于定量。 在一些实施方案中，使用一种含有亲二烯体的标记的狄尔斯·阿德尔试剂的一步标签试剂。