公开(公告)号：US10344322B2

公开(公告)日：2019-07-09

申请号：US15249684

申请日：2016-08-29

Applicant: Dow AgroSciences LLC

Inventor： Lakshmi Sastry-Dent ,  Matthew A. Simpson ,  Zehui Cao ,  Wei Chen ,  Ning Zhou ,  Steven R. Webb

IPC: C12Q1/68 ,  C12Q1/6858 ,  C12Q1/6895 ,  C12N15/10 ,  C12N15/66 ,  C12N15/70 ,  C40B40/08 ,  A01G24/00 ,  C12N5/00

Abstract: The present disclosure provides methods for detecting and identifying plant events that contain precision targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method can be deployed as a high throughput process utilized for screening the intactness or disruption of a targeted genomic loci and optionally for detecting a donor DNA polynucleotide insertion at the targeted genomic loci. The methods are readily applicable for the identification of plant events produced via a targeting method which results from the use of a site specific nuclease.

公开(公告)号：US20180163218A1

公开(公告)日：2018-06-14

申请号：US15833168

申请日：2017-12-06

Applicant: Dow AgroSciences LLC

Inventor： David H. Corbin ,  Wei Chen ,  Stephen Novak ,  Ryan M. Lee ,  Sandeep Kumar ,  Andrew Asberry ,  Andrew F. Worden

IPC: C12N15/82 ,  C12N9/22

Abstract: Disclosed herein are methods and compositions for the repair of site specific nuclease binding sites by targeted integration and/or targeted excision of one or more sequences into a cell.

公开(公告)号：US20170334957A1

公开(公告)日：2017-11-23

申请号：US15485338

申请日：2017-04-12

Applicant: Dow AgroSciences LLC

Inventor： Manju Gupta ,  Sandeep Kumar ,  Wei Chen

IPC: C07K14/415 ,  C12N15/82 ,  C12N15/00

CPC classification number: C12N15/8222 ,  C12N15/00 ,  C12N15/8216 ,  C12N15/8225 ,  C12N15/8241 ,  C12N15/8286 ,  Y02A40/162

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a Zea mays GRMZM2G047720 promoter. Some embodiments relate to a Zea mays GRMZM2G047720 promoter that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a Zea mays GRMZM2G047720 3′UTR that functions in plants to terminate transcription of operably linked nucleotide sequences.

公开(公告)号：US09725773B2

公开(公告)日：2017-08-08

申请号：US14879294

申请日：2015-10-09

Applicant: Dow AgroSciences LLC

Inventor： Wei Chen ,  Tiffany King ,  Yanxin Star Gao ,  Jafar Mammadov

IPC: C12Q1/68 ,  A01H1/04 ,  A01H5/10

CPC classification number: C12Q1/6895 ,  A01H1/04 ,  A01H5/10 ,  C12Q2600/13 ,  C12Q2600/156

Abstract: This invention relates to methods for identifying maize plants that having increased green snap tolerance. The methods use molecular markers to identify and to select plants with increased green snap tolerance. Maize plants generated by the methods of the invention are also a feature of the invention.

公开(公告)号：US20160130594A1

公开(公告)日：2016-05-12

申请号：US14938632

申请日：2015-11-11

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Jeffrey R. Berringer ,  Wei Chen ,  Andrew M. Asberry

IPC: C12N15/82

CPC classification number: C12N15/8216 ,  C12N15/8209 ,  C12N15/8286 ,  Y02A40/162

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a minimal core promoter element from a Zea mays Ubiquitin-1 gene promoter, and the full-length nucleotide sequence elements from a Rice Ubiquitin-3 promoter. Some embodiments relate to a synthetic bi-directional promoter that may function in plants to promote transcription of two operably linked nucleotide sequences.

Abstract translation: 本公开涉及用于促进植物或植物细胞中核苷酸序列的转录的组合物和方法，其使用来自玉米泛素-1基因启动子的最小核心启动子元件和来自水稻泛素-3的全长核苷酸序列元件 启动子。 一些实施方案涉及可在植物中起作用以促进两个可操作地连接的核苷酸序列的转录的合成双向启动子。

公开(公告)号：US20160102370A1

公开(公告)日：2016-04-14

申请号：US14879294

申请日：2015-10-09

Applicant: Dow AgroSciences LLC

Inventor： Wei Chen ,  Tiffany King ,  Yanxin Star Gao ,  Jafar Mammadov

IPC: C12Q1/68 ,  C12N15/82

CPC classification number: C12Q1/6895 ,  A01H1/04 ,  A01H5/10 ,  C12Q2600/13 ,  C12Q2600/156

Abstract: This invention relates to methods for identifying maize plants that having increased green snap tolerance. The methods use molecular markers to identify and to select plants with increased green snap tolerance. Maize plants generated by the methods of the invention are also a feature of the invention.

Abstract translation: 本发明涉及鉴定具有增加的绿色捕捉耐受性的玉米植物的方法。 该方法使用分子标记来鉴定和选择具有增加的绿色捕捉耐受性的植物。 通过本发明的方法产生的玉米植物也是本发明的特征。

公开(公告)号：US20150167105A1

公开(公告)日：2015-06-18

申请号：US14561496

申请日：2014-12-05

Applicant: DOW AGROSCIENCES LLC

Inventor： Jafar Mammadov ,  Jerry R. Rice ,  Wei Chen ,  Yanxin Star Gao ,  Joseph T. Metzler ,  Ruihua Ren

IPC: C12Q1/68 ,  A01H5/10 ,  A01H1/04

CPC classification number: C12Q1/6895 ,  A01H1/04 ,  A01H5/10 ,  C12Q2600/13 ,  C12Q2600/156 ,  C12Q2600/16

Abstract: This invention relates to methods for identifying maize plants that have decreased gray leaf spot. The methods use molecular markers to identify and to select plants with decreased gray leaf spot or to identify and deselect plants with increased gray leaf spot. Maize plants generated by the methods of the invention are also a feature of the invention.

Abstract translation: 本发明涉及鉴定具有降低的灰叶斑点的玉米植物的方法。 该方法使用分子标记来鉴定和选择具有降低的灰叶斑点的植物，或鉴定和取消植物具有增加的灰叶斑点。 通过本发明的方法产生的玉米植物也是本发明的特征。

公开(公告)号：US10400245B2

公开(公告)日：2019-09-03

申请号：US15485338

申请日：2017-04-12

Applicant: Dow AgroSciences LLC

Inventor： Manju Gupta ,  Sandeep Kumar ,  Wei Chen

IPC: C12N15/82 ,  C12N15/00

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a Zea mays GRMZM2G047720 promoter. Some embodiments relate to a Zea mays GRMZM2G047720 promoter that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a Zea mays GRMZM2G047720 3′UTR that functions in plants to terminate transcription of operably linked nucleotide sequences.

公开(公告)号：US20190002905A9

公开(公告)日：2019-01-03

申请号：US14938632

申请日：2015-11-11

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Jeffrey R. Beringer ,  Wei Chen ,  Andrew M. Asberry

IPC: C12N15/82

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a minimal core promoter element from a Zea mays Ubiquitin-1 gene promoter, and the full-length nucleotide sequence elements from a Rice Ubiquitin-3 promoter. Some embodiments relate to a synthetic bi-directional promoter that may function in plants to promote transcription of two operably linked nucleotide sequences.

公开(公告)号：US09988638B2

公开(公告)日：2018-06-05

申请号：US14938643

申请日：2015-11-11

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Toby Cicak ,  Heather Leigh Robinson ,  Dayakar Reddy Pareddy ,  Wei Chen

IPC: C12N15/82

CPC classification number: C12N15/8216

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a minimal core promoter element from a Arabidopsis thaliana Ubiquitin-10 gene promoter or Cassava Vein Mosaic Virus promoter, and the full-length nucleotide sequence elements from a Cassava Vein Mosaic Virus promoter. Some embodiments relate to a synthetic CsVMV bi-directional promoter that functions in plants to promote transcription of two operably linked nucleotide sequences.