摘要:
A method and system for performing biochemical detection or analysis on micro- and nano-scale subcellular component within a single biological cell is provided. An integrated platform device and method to perform the biochemical analysis is also provided.
摘要:
A method for quantifying fluorescent puncta comprising acquiring at least one first intensity distribution comprising fluorescence intensity values from a plurality of first fluorescent puncta; acquiring at least one second intensity distribution comprising fluorescence intensity values from a plurality of second fluorescent puncta, wherein each second fluorescent puncta has a determined number of fluorescent emitters; determining the relationship between the first and second intensity distributions; and fitting the second intensity distribution to the first intensity distribution to provide a count and distribution of the number of fluorescent emitters within the first fluorescent puncta.
摘要:
Embodiments of the present invention employ complexly shaped, high-surface-area channels for separation and purification of molecules, including important biopolymers such as proteins, glycoproteins, polysaccharides, and other molecular components of living cells. The relatively large internal surface areas of the complexly shaped channels employed in embodiments of the present invention provide, in comparison to traditional, simply shaped separation channels, increased heat dissipation during electrokinetic separation, and a decreased tendency for bulk-solution flow. Heat dissipation prevents high temperatures that can denature proteins and that can induce thermal currents within the separation channel. Bulk-solution flow within a separation channel can overwhelm the generally linear, electrical-potential-induced migration of molecules that leads to efficient and well-resolved molecular separations. The complexly shaped channels employed in various embodiments of the present invention can be readily manufactured at microscale dimensions for use in microscale devices, at millimeter-scale dimensions for inclusion in microfluidics devices, and may also be used in larger scale, traditional separation and purification systems.
摘要:
This document discloses, among other things, a method and system for a substrate having a bypass region for fluid flow. The substrate includes a plurality of fluid flow channels with each channel configured to concurrently allow fluid flow while precluding passage of a target particle or object.
摘要:
The flexible through-connection process, operational in a Mobile Switch Center, that provides support for allowing the call routing processor of the Mobile Switch Center to independently perform a through-connection/switch-connection based on different types of calls so that the Mobile Switch Center can make a through-connection at different stages of the outgoing call leg. In operation, the present flexible through-connection process includes in the call control processor of the switching system a new parameter in the existing inter-process message which is sent to the call routing processor at call setup time. The values supported for this new parameter will be pre-defined in the Mobile Switch Center and used by the call routing processor to determine when to perform through-connection/switch-connection for an outgoing call leg. When the call routing processor routs the call out and the outgoing call is at the stage, in which the value of the parameter received is defined for, it performs a through-connection operation. Additionally this flexible through-connection process automatically disconnects the other call leg, i.e., switches the connection from one called leg to another called leg when multiple call legs are involved for an incoming call.
摘要:
This invention relates to a detachable castor support for a baby carriage and in particular to one including a body portion having a vertical shaft with a pivot hole and a protuberance, a stand having a rectangular recess having two sides each formed with a semicircular hole at the lower part thereof and an elongated slot at the front part thereof, a U-shaped member extending from the rear part of the stand and having a hole in each vertically extending sidewall of the U-shaped member and a semicircular notch at the bottom thereof, a connector disposed within the stand with its tubular lug engaged with the semicircular notch of the stand and its protuberance fitted into the elongated slot of the stand and a rivet extending through the hole of the U-shaped member and the lower part of the vertical shaft of the body portion whereby the baby carriage can be stowed conveniently and the castor thereof can be easily replaced with a new one once damaged.