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22 条记录 (耗时 0.033 秒)

    Method and system for electrokinetic, preparative sample separation in high-surface-area separation channels with non-convex cross sections
    1.
    发明申请
    Method and system for electrokinetic, preparative sample separation in high-surface-area separation channels with non-convex cross sections 失效
    在具有非凸截面的高表面积分离通道中电动，制备样品分离的方法和系统

    公开(公告)号：US20050003411A1

    公开(公告)日：2005-01-06

    申请号：US10846147

    申请日：2004-05-14

    申请人： Daniel Chiu ,  Jason Kuo ,  David Lim

    发明人： Daniel Chiu ,  Jason Kuo ,  David Lim

    IPC分类号： B01D20060101 ,  B29C53/00 ,  C12M1/34 ,  C12Q1/68 ,  G01N23/20 ,  G01N27/453

    CPC分类号： G01N23/20

    摘要： Embodiments of the present invention employ complexly shaped, high-surface-area channels for separation and purification of molecules, including important biopolymers such as proteins, glycoproteins, polysaccharides, and other molecular components of living cells. The relatively large internal surface areas of the complexly shaped channels employed in embodiments of the present invention provide, in comparison to traditional, simply shaped separation channels, increased heat dissipation during electrokinetic separation, and a decreased tendency for bulk-solution flow. Heat dissipation prevents high temperatures that can denature proteins and that can induce thermal currents within the separation channel. Bulk-solution flow within a separation channel can overwhelm the generally linear, electrical-potential-induced migration of molecules that leads to efficient and well-resolved molecular separations. The complexly shaped channels employed in various embodiments of the present invention can be readily manufactured at microscale dimensions for use in microscale devices, at millimeter-scale dimensions for inclusion in microfluidics devices, and may also be used in larger scale, traditional separation and purification systems.

    摘要翻译： 本发明的实施方案采用复杂形状的高表面积通道用于分离和纯化分子，包括重要的生物聚合物如蛋白质，糖蛋白，多糖和活细胞的其它分子组分。 与传统的简单成型的分离通道相比，在本发明的实施方案中采用的复杂形状的通道的相对较大的内表面积提供了电动分离期间增加的散热性和体积溶液流动的趋势。 散热可以预防高温，使蛋白质变性，并能在分离通道内引起热流。 分离通道内的体积溶液流可以压倒一般线性的电位诱导的分子迁移，导致有效和良好的分解分离。 在本发明的各种实施方案中使用的复杂形状的通道可以容易地以微尺度制造用于微尺寸装置，以毫米级尺寸包含在微流体装置中，并且还可用于较大规模的传统分离和净化系统 。

    Method for electro-permeabilization of individual cellular and organellar structures and use thereof
    9.
    发明授权
    Method for electro-permeabilization of individual cellular and organellar structures and use thereof 有权
    单个细胞和细胞器结构的电渗透方法及其用途

    公开(公告)号：US06521430B1

    公开(公告)日：2003-02-18

    申请号：US09557979

    申请日：2000-04-25

    申请人： Owe Orwar ,  Anders Lundqvist ,  Peter Eriksson ,  Daniel Chiu

    发明人： Owe Orwar ,  Anders Lundqvist ,  Peter Eriksson ,  Daniel Chiu

    IPC分类号： C12N1300

    CPC分类号： A61N1/327 ,  A61N1/306 ,  C12M35/02 ,  C12N13/00

    摘要： The invention relates to a method for permeabilization of a cell structure consisting of a single cell, an intracellular structure or an organelle comprising the following steps: (a) microelectrodes, preferably two carbon fibre electrodes or hollow fibre electrodes, are provided, (b) the microelectrodes are connected to a power supply, (c) the electrodes, individually controlled by high-graduation micromanipulators, are placed close to the cell structure at an appropriate inter-electrode distance, and (d) a highly focused electric field of a strength sufficient to obtain electroporation is applied between the electrodes. The method may be used in order to transfer cell impermeant solutes, such as drugs or genes, into the cell structure or out of the cell structure, in biosensors, in the treatment of tumours and neurodegenerative diseases and in the study of biophysical processes.

    摘要翻译： 本发明涉及一种由单细胞，细胞内结构或细胞器组成的细胞结构透化的方法，包括以下步骤：（a）提供微电极，优选两个碳纤维电极或中空纤维电极，（b） 微电极连接到电源，（c）由高分辨率显微操纵器单独控制的电极以适当的电极间距放置在电池结构附近，以及（d）强度高的聚焦电场 足以获得电穿孔的电极。 可以使用该方法以将细胞不透性溶质（例如药物或基因）转移到细胞结构或细胞结构，生物传感器中，在治疗肿瘤和神经变性疾病中以及在生物物理过程的研究中。