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    Codon optimized synthetic plasmids
    2.
    发明授权
    Codon optimized synthetic plasmids 有权
    密码子优化的合成质粒

    公开(公告)号：US07316925B2

    公开(公告)日：2008-01-08

    申请号：US10619939

    申请日：2003-07-15

    申请人： Ruxandra Draghia-Akli ,  Ronald V. Abruzzese ,  Douglas R. Kern

    发明人： Ruxandra Draghia-Akli ,  Ronald V. Abruzzese ,  Douglas R. Kern

    IPC分类号： C12N15/85 ,  C07H21/04 ,  A61K48/00

    CPC分类号： C12N15/67 ,  A61K48/00 ,  C07K14/60 ,  C12N15/85 ,  C12N2830/008 ,  C12N2830/15 ,  C12N2840/20 ,  C12N2840/203

    摘要： One aspect of the current invention is an optimized synthetic mammalian expression plasmid (e.g. pAV0201). This new plasmid comprise a therapeutic element, and a replication element. The therapeutic element of the new plasmid comprises a eukaryotic promoter; a 5′ untranslated region (“UTR”); a codon-optimized-eukaryotic therapeutic gene sequence; and a poly adenylation signal. The therapeutic elements of this plasmid are operatively linked and located in a first operatively-linked arrangement. Additionally, the optimized synthetic mammalian expression plasmid comprises replication elements, wherein the replication elements are operatively linked and located in a second operatively-linked arrangement. The replication elements comprise a selectable marker gene promoter, a ribosomal binding site, and an origin of replication. The first-operatively-linked arrangement and the second-operatively-linked arrangement comprise a circular structure of the codon optimized synthetic mammalian expression plasmid.

    摘要翻译： 本发明的一个方面是优化的合成哺乳动物表达质粒（例如pAV0201）。 该新质粒包含治疗元件和复制元件。 新质粒的治疗元件包含真核启动子; 5'非翻译区（“UTR”）; 密码子优化的真核治疗基因序列; 和聚腺苷酸化信号。 该质粒的治疗元件可操作地连接并位于第一可操作连接的布置中。 此外，优化的合成哺乳动物表达质粒包含复制元件，其中复制元件可操作地连接并位于第二可操作连接的布置中。 复制元件包括选择性标记基因启动子，核糖体结合位点和复制起点。 第一操作连接的排列和第二操作连接的排列包括密码子优化的合成哺乳动物表达质粒的圆形结构。

    Electrode assembly for constant-current electroporation and use
    3.
    发明授权
    Electrode assembly for constant-current electroporation and use 有权
    用于恒流电穿孔和使用的电极组件

    公开(公告)号：US07664545B2

    公开(公告)日：2010-02-16

    申请号：US11495021

    申请日：2006-07-28

    申请人： Allan Westersten ,  William R. Wilkinson ,  Ruxandra Draghia-Akli ,  Robert H. Carpenter ,  Douglas R. Kern

    发明人： Allan Westersten ,  William R. Wilkinson ,  Ruxandra Draghia-Akli ,  Robert H. Carpenter ,  Douglas R. Kern

    IPC分类号： A61N1/30

    CPC分类号： A61N1/327

    摘要： The present invention relates to a modular electrode system, and its use, for facilitating the introduction of a macromolecule into cells of a selected tissue in a body or plant. The modular electrode system comprises a non-symmetrically arranged plurality of needle electrodes; a hypodermic needle; an electrical connector that provides a conductive link from a programmable constant-current pulse controller to the plurality of needle electrodes; and a power source. In a preferred embodiment of the present invention, an operator can grasp the plurality of needle electrodes that are mounted on a support structure and firmly insert the them into the selected tissue in a body or plant. The macromolecules are then delivered via the hypodermic needle into the selected tissue. The programmable constant-current pulse controller is activated and constant-current electrical pulse is applied to the plurality of needle electrodes. The applied constant-current electrical pulse facilitates the introduction of the macromolecule into the cell between the plurality of electrodes. Cell death due to overheating of cells is minimized by limiting the power dissipation in the tissue by virtue of constant-current pulses.

    摘要翻译： 本发明涉及一种模块化电极系统及其用途，用于促进将大分子引入体内或选定组织中的细胞中。 模块化电极系统包括非对称布置的多个针电极; 皮下注射针 电连接器，其从可编程恒定电流脉冲控制器提供到多个针电极的导电连接; 和电源。 在本发明的优选实施例中，操作者可以抓住安装在支撑结构上的多个针电极并将其牢固地插入到身体或植物中的所选择的组织中。 然后通过皮下注射针将大分子递送到选定的组织中。 可编程恒定电流脉冲控制器被激活，并且多个针电极施加恒流电脉冲。 施加的恒流电脉冲有助于将大分子引入多个电极之间的电池中。 由于通过恒定电流脉冲限制组织中的功率消耗，使由于细胞过热引起的细胞死亡被最小化。

    Plasmid mediated supplementation for treating chronically ill subjects
    4.
    发明申请
    Plasmid mediated supplementation for treating chronically ill subjects 有权
    质粒介导的补充治疗慢性病患者

    公开(公告)号：US20090170748A1

    公开(公告)日：2009-07-02

    申请号：US11726291

    申请日：2007-03-21

    申请人： Ruxandra Draghia-Akli ,  Robert H. Carpenter ,  Douglas R. Kern ,  Robert J. Schwartz ,  Glen King ,  Kevin Hahn ,  Malcolm K. Brenner

    发明人： Ruxandra Draghia-Akli ,  Robert H. Carpenter ,  Douglas R. Kern ,  Robert J. Schwartz ,  Glen King ,  Kevin Hahn ,  Malcolm K. Brenner

    IPC分类号： A61K38/00 ,  A61P7/06

    CPC分类号： A61K38/25 ,  A61K48/00

    摘要： The present invention pertains to compositions and methods for plasmid-mediated supplementation. The compositions and methods are useful for treating anemia and other effects that are commonly associated in cancer bearing animals. Overall, the embodiments of the invention can be accomplished by delivering an effective amount of a nucleic acid expression construct that encodes a GHRH or functional biological equivalent thereof into a tissue of an animal and allowing expression of the encoded gene in the animal. For example, when such a nucleic acid sequence is delivered into the specific cells of the animal tissue specific constitutive expression is achieved.

    摘要翻译： 本发明涉及用于质粒介导的补充的组合物和方法。 组合物和方法可用于治疗贫血和通常与携带癌症的动物相关的其它作用。 总的来说，本发明的实施方案可以通过将有效量的编码GHRH或其功能生物等价物的核酸表达构建体递送到动物的组织中并允许编码的基因在动物中表达来实现。 例如，当这种核酸序列被递送到动物组织的特定细胞中时，实现了特异性组成型表达。

    CODON OPTIMIZED SYNTHETIC PLASMIDS
    7.
    发明申请
    CODON OPTIMIZED SYNTHETIC PLASMIDS 审中-公开
    CODON优化合成PLASMIDS

    公开(公告)号：US20080194511A1

    公开(公告)日：2008-08-14

    申请号：US11941876

    申请日：2007-11-16

    申请人： Ruxandra Draghia-Akli ,  Ronald V. Abruzzese ,  Douglas R. Kern

    发明人： Ruxandra Draghia-Akli ,  Ronald V. Abruzzese ,  Douglas R. Kern

    IPC分类号： A61K31/711

    CPC分类号： C12N15/67 ,  A61K48/00 ,  C07K14/60 ,  C12N15/85 ,  C12N2830/008 ,  C12N2830/15 ,  C12N2840/20 ,  C12N2840/203

    摘要： One aspect of the current invention is an optimized synthetic mammalian expression plasmid (e.g. pAV0201). This new plasmid comprise a therapeutic element, and a replication element. The therapeutic element of the new plasmid comprises a eukaryotic promoter; a 5′ untranslated region (“UTR”); a codon-optimized-eukaryotic therapeutic gene sequence; and a poly adenylation signal. The therapeutic elements of this plasmid are operatively linked and located in a first operatively-linked arrangement. Additionally, the optimized synthetic mammalian expression plasmid comprises replication elements, wherein the replication elements are operatively linked and located in a second operatively-linked arrangement. The replication elements comprise a selectable marker gene promoter, a ribosomal binding site, and an origin of replication. The first-operatively-linked arrangement and the second-operatively-linked arrangement comprise a circular structure of the codon optimized synthetic mammalian expression plasmid.

    摘要翻译： 本发明的一个方面是优化的合成哺乳动物表达质粒（例如pAV0201）。 该新质粒包含治疗元件和复制元件。 新质粒的治疗元件包含真核启动子; 5'非翻译区（“UTR”）; 密码子优化的真核治疗基因序列; 和聚腺苷酸化信号。 该质粒的治疗元件可操作地连接并位于第一可操作连接的布置中。 此外，优化的合成哺乳动物表达质粒包含复制元件，其中复制元件可操作地连接并位于第二可操作连接的布置中。 复制元件包括选择性标记基因启动子，核糖体结合位点和复制起点。 第一操作连接的排列和第二操作连接的排列包括密码子优化的合成哺乳动物表达质粒的圆形结构。

    Electrode assembly for constant-current electroporation and use
    8.
    发明授权
    Electrode assembly for constant-current electroporation and use 有权
    用于恒流电穿孔和使用的电极组件

    公开(公告)号：US07245963B2

    公开(公告)日：2007-07-17

    申请号：US10360768

    申请日：2002-03-07

    申请人： Ruxandra Draghia-Akli ,  Robert H. Carpenter ,  Douglas R. Kern ,  Allan Westersten ,  William R. Wilkinson

    发明人： Ruxandra Draghia-Akli ,  Robert H. Carpenter ,  Douglas R. Kern ,  Allan Westersten ,  William R. Wilkinson

    IPC分类号： A61N1/30

    CPC分类号： A61N1/327

    摘要： The present invention relates to a modular electrode system, and its use, for facilitating the introduction of a macromolecule into cells of a selected tissue in a body or plant. The modular electrode system comprises a plurality of needle electrodes; a hypodermic needle; an electrical connector that provides a conductive link from a programmable constant-current pulse controller to the plurality of needle electrodes; and a power source. In a preferred embodiment of the present invention, an operator can grasp the plurality of needle electrodes that are mounted on a support structure and firmly insert the them into the selected tissue in a body or plant. The macromolecules are then delivered via the hypodermic needle into the selected tissue. The programmable constant-current pulse controller is activated and constant-current electrical pulse is applied to the plurality of needle electrodes. The applied constant-current electrical pulse facilitates the introduction of the macromolecule into the cell between the plurality of electrodes. Cell death due to overheating of cells is minimized by limiting the power dissipation in the tissue by virtue of constant-current pulses.

    摘要翻译： 本发明涉及一种模块化电极系统及其用途，用于促进将大分子引入体内或选定组织中的细胞中。 模块化电极系统包括多个针电极; 皮下注射针 电连接器，其从可编程恒定电流脉冲控制器提供到多个针电极的导电连接; 和电源。 在本发明的优选实施例中，操作者可以抓住安装在支撑结构上的多个针电极并将其牢固地插入到身体或植物中的所选择的组织中。 然后通过皮下注射针将大分子递送到选定的组织中。 可编程恒定电流脉冲控制器被激活，并且多个针电极施加恒流电脉冲。 施加的恒流电脉冲有助于将大分子引入多个电极之间的电池中。 由于通过恒定电流脉冲限制组织中的功率消耗，使由于细胞过热引起的细胞死亡被最小化。