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    Nuclear thyroid hormone receptor-interacting polynucleotides and related molecules and methods
    1.
    发明授权
    Nuclear thyroid hormone receptor-interacting polynucleotides and related molecules and methods 失效
    核甲状腺激素受体相互作用多核苷酸及相关分子及方法

    公开(公告)号：US5962256A

    公开(公告)日：1999-10-05

    申请号：US471613

    申请日：1995-06-06

    申请人： David D. Moore ,  Jae Woon Lee

    发明人： David D. Moore ,  Jae Woon Lee

    IPC分类号： G01N33/566 ,  A61K38/00 ,  A61P5/12 ,  C07H21/04 ,  C07K14/47 ,  C07K14/72 ,  C12N1/21 ,  C12N5/10 ,  C12N15/09 ,  C12N15/10 ,  C12N15/62 ,  C12P21/02 ,  C12Q1/02 ,  C12Q1/68 ,  C12N1/15

    CPC分类号： C07K14/721 ,  C07K14/47 ,  C12N15/1034 ,  C12N15/62 ,  C12Q1/6897 ,  A61K38/00 ,  C07K2319/00 ,  C07K2319/71 ,  C07K2319/715 ,  C07K2319/80

    摘要： Disclosed is a method for determining whether a test protein is capable of interacting with a nuclear hormone receptor protein. The method involves: (a) providing a host cell which contains (i) a reporter gene operably linked to a protein binding site; (ii) a first fusion gene which expresses a first fusion protein, the first fusion protein including a nuclear hormone receptor protein covalently bonded to a binding moiety which is capable of specifically binding to the protein binding site; and (iii) a second fusion gene which expresses a second fusion protein, the second fusion protein including the test protein covalently bonded to a weak gene activating moiety; and (b) determining whether the test protein increases expression of the reporter gene as an indication of its ability to interact with the nuclear hormone receptor protein. Such an interaction may be hormone dependent, hormone independnet, or hormone sensitive. Also disclosed is purified DNA encoding thyroid hormone receptor-interacting proteins and the polypeptides expressed from such DNA.

    摘要翻译： 公开了一种用于确定测试蛋白质是否能够与核激素受体蛋白质相互作用的方法。 该方法包括：（a）提供宿主细胞，其包含（i）可操作地连接到蛋白质结合位点的报告基因; （ii）表达第一融合蛋白的第一融合基因，所述第一融合蛋白包括共价键合到能够特异性结合蛋白结合位点的结合部分的核激素受体蛋白; 和（iii）表达第二融合蛋白的第二融合基因，所述第二融合蛋白包括与弱基因激活部分共价结合的测试蛋白; 和（b）确定测试蛋白质是否增加了报告基因的表达，以表明其与核激素受体蛋白质相互作用的能力。 这种相互作用可以是激素依赖性的，激素独立的或激素敏感的。 还公开了编码甲状腺激素受体相互作用蛋白质的纯化DNA和从这种DNA表达的多肽。

    Nuclear thyroid hormone receptor-interacting polypeptides and related molecules and methods
    2.
    发明授权
    Nuclear thyroid hormone receptor-interacting polypeptides and related molecules and methods 失效
    核甲状腺激素受体相互作用多肽及相关分子及方法

    公开(公告)号：US5866686A

    公开(公告)日：1999-02-02

    申请号：US470925

    申请日：1995-06-06

    申请人： David D. Moore ,  Jae Woon Lee

    发明人： David D. Moore ,  Jae Woon Lee

    IPC分类号： G01N33/566 ,  A61K38/00 ,  A61P5/12 ,  C07H21/04 ,  C07K14/47 ,  C07K14/72 ,  C12N1/21 ,  C12N5/10 ,  C12N15/09 ,  C12N15/10 ,  C12N15/62 ,  C12P21/02 ,  C12Q1/02 ,  C12Q1/68 ,  A61K38/16

    CPC分类号： C07K14/721 ,  C07K14/47 ,  C12N15/1034 ,  C12N15/62 ,  C12Q1/6897 ,  A61K38/00 ,  C07K2319/00 ,  C07K2319/71 ,  C07K2319/715 ,  C07K2319/80

    摘要： Disclosed is a method for determining whether a test protein is capable of interacting with a nuclear hormone receptor protein. The method involves: (a) providing a host cell which contains (i) a reporter gene operably linked to a protein binding site; (ii) a first fusion gene which expresses a first fusion protein, the first fusion protein including a nuclear hormone receptor protein covalently bonded to a binding moiety which is capable of specifically binding to the protein binding site; and (iii) a second fusion gene which expresses a second fusion protein, the second fusion protein including the test protein covalently bonded to a weak gene activating moiety; and (b) determining whether the test protein increases expression of the reporter gene as an indication of its ability to interact with the nuclear hormone receptor protein. Such an interaction may be hormone dependent, hormone independent, or hormone sensitive. Also disclosed is purified DNA encoding thyroid hormone receptor-interacting proteins and the polypeptides expressed from such DNA.

    摘要翻译： 公开了一种用于确定测试蛋白质是否能够与核激素受体蛋白质相互作用的方法。 该方法包括：（a）提供宿主细胞，其包含（i）可操作地连接到蛋白质结合位点的报告基因; （ii）表达第一融合蛋白的第一融合基因，所述第一融合蛋白包括共价键合到能够特异性结合蛋白结合位点的结合部分的核激素受体蛋白; 和（iii）表达第二融合蛋白的第二融合基因，所述第二融合蛋白包括与弱基因激活部分共价结合的测试蛋白; 和（b）确定测试蛋白质是否增加了报告基因的表达，以表明其与核激素受体蛋白质相互作用的能力。 这种相互作用可以是激素依赖性的，与激素无关的或激素敏感的。 还公开了编码甲状腺激素受体相互作用蛋白质的纯化DNA和从这种DNA表达的多肽。

    Nuclear hormone receptor-interacting polypeptides and related molecules and methods
    3.
    发明授权
    Nuclear hormone receptor-interacting polypeptides and related molecules and methods 失效
    核激素受体相互作用的多肽和相关分子和方法

    公开(公告)号：US5846711A

    公开(公告)日：1998-12-08

    申请号：US222719

    申请日：1994-04-04

    申请人： David D. Moore ,  Jae Woon Lee

    发明人： David D. Moore ,  Jae Woon Lee

    IPC分类号： G01N33/566 ,  A61K38/00 ,  A61P5/12 ,  C07H21/04 ,  C07K14/47 ,  C07K14/72 ,  C12N1/21 ,  C12N5/10 ,  C12N15/09 ,  C12N15/10 ,  C12N15/62 ,  C12P21/02 ,  C12Q1/02 ,  C12Q1/68 ,  C07H17/00 ,  C12N15/00 ,  C12P21/06

    CPC分类号： C07K14/721 ,  C07K14/47 ,  C12N15/1034 ,  C12N15/62 ,  C12Q1/6897 ,  A61K38/00 ,  C07K2319/00 ,  C07K2319/71 ,  C07K2319/715 ,  C07K2319/80

    摘要： Disclosed is a method for determining whether a test protein is capable of interacting with a nuclear hormone receptor protein. The method involves: (a) providing a host cell which contains (i) a reporter gene operably linked to a protein binding site; (ii) a first fusion gene which expresses a first fusion protein, the first fusion protein including a nuclear hormone receptor protein covalently bonded to a binding moiety which is capable of specifically binding to the protein binding site; and (iii) a second fusion gene which expresses a second fusion protein, the second fusion protein including the test protein covalently bonded to a weak gene activating moiety; and (b) determining whether the test protein increases expression of the reporter gene as an indication of its ability to interact with the nuclear hormone receptor protein. Such an interaction may be hormone dependent, hormone independent, or hormone sensitive. Also disclosed is purified DNA encoding thyroid hormone receptor-interacting proteins and the polypeptides expressed from such DNA.

    摘要翻译： 公开的是用于确定测试蛋白是否能够与核激素受体蛋白质相互作用的方法。 该方法包括：（a）提供宿主细胞，其包含（i）可操作地连接到蛋白质结合位点的报告基因; （ⅱ），其表达的第一融合蛋白，所述第一融合蛋白，包括共价键合到其能够特异性地结合蛋白结合位点的结合部分核激素受体蛋白质的第一融合基因; 及（iii）表达第二融合蛋白，所述第二融合蛋白，包括共价键合弱基因激活部分上的测试蛋白质的第二融合基因; 和（b）确定所述测试蛋白质是否增加了报告基因的表达作为其与核激素受体蛋白质相互作用的能力的指示。 这种相互作用可以是激素依赖性，激素非依赖性或激素敏感。 还公开了纯化的DNA编码甲状腺激素受体相互作用蛋白以及由这种DNA表达的多肽。

    PHOSPHOLIPID COMPOSITIONS AND USES THEREOF
    7.
    发明申请
    PHOSPHOLIPID COMPOSITIONS AND USES THEREOF 失效
    磷脂组合物及其用途

    公开(公告)号：US20100331281A1

    公开(公告)日：2010-12-30

    申请号：US12742879

    申请日：2008-11-17

    申请人： David D. Moore ,  Jae Man Lee

    发明人： David D. Moore ,  Jae Man Lee

    IPC分类号： A61K31/661 ,  C07F9/10 ,  A61P3/04 ,  A61P3/10

    CPC分类号： A61K31/661

    摘要： We have discovered that phospholipids diundecanoyl (C11:0-C11:0) and dilauroyl (C12:0-C12:0) phosphatidylcholine (PC) act as agonists of the LRH-1 receptor. We have also shown that administration of these lipids to diabetic mice reduces blood glucose levels. On the basis of these discoveries, the present invention features compositions that include these lipids and structurally related lipids. Also featured are methods of treating metabolic disorders and inflammatory bowel disease, lowering blood glucose levels, and increasing LRH-1 receptor activity in a subject by administration of these lipids.

    摘要翻译： 我们已经发现磷脂二十一酰基（C11：0-C11：0）和二月桂酰（C12：0-C12：0）磷脂酰胆碱（PC）作为LRH-1受体的激动剂。 我们还表明，将这些脂质给予糖尿病小鼠降低血糖水平。 基于这些发现，本发明的特征在于包括这些脂质和结构相关脂质的组合物。 还特征在于通过施用这些脂质来治疗代谢紊乱和炎性肠病，降低血糖水平和增加受试者中的LRH-1受体活性的方法。

    Mechanical battery
    8.
    发明授权
    Mechanical battery 有权

    公开(公告)号：US10688855B1

    公开(公告)日：2020-06-23

    申请号：US16224563

    申请日：2018-12-18

    申请人： David D. Moore

    发明人： David D. Moore

    IPC分类号： B60K1/00 ,  B60K1/04 ,  H01M2/20 ,  H01M6/50 ,  H01M2/10 ,  G01R31/3835 ,  H01M10/42

    摘要： An energy storage system may include an internal battery. Further, the energy storage system may include a motor electrically coupled to the internal battery. Further, the energy storage system may include an alternator rotatably coupled with the motor. Further, the energy storage system may include a primary battery electrically coupled to the alternator. Further, the energy storage system may include a voltage sensor configured for sensing a voltage level of the primary battery. Further, the energy storage system may include a switching device electrically coupled to each of the internal battery and the motor; and, may be configured for controlling electrical connectivity between the internal battery and the motor based on the voltage level of the primary battery. Further, the energy storage system may include an electrical interconnect electrically coupled to one or more of the internal battery, the alternator and the primary battery.