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12 条记录 (耗时 0.022 秒)

    Whole blood preparation for cytometric analysis of cell signaling pathways
    7.
    发明授权
    Whole blood preparation for cytometric analysis of cell signaling pathways 有权
    全血制备细胞信号传导途径的细胞分析

    公开(公告)号：US07803523B2

    公开(公告)日：2010-09-28

    申请号：US10928570

    申请日：2004-08-27

    申请人： Sue Chow ,  David Hedley ,  T. Vincent Shankey ,  Patricia Grom

    发明人： Sue Chow ,  David Hedley ,  T. Vincent Shankey ,  Patricia Grom

    IPC分类号： A01N1/02 ,  G01N1/00

    CPC分类号： G01N33/80 ,  Y10T436/107497 ,  Y10T436/108331 ,  Y10T436/25 ,  Y10T436/25125 ,  Y10T436/2525 ,  Y10T436/25375

    摘要： This invention is directed to a method for preparation of a biological sample for measurement of protein epitopes that allows for the preservation of intracellular protein epitopes and detection of signal transduction pathways based on the ability to capture transient activation states of the epitopes. The method provided by the invention allows for the rapid fixation of biological samples containing red blood cells, to ensure that epitopes of signal transduction molecules and other intracellular protein epitopes are preserved in the active state. The method of the invention further allows for lysis of red blood cells, thereby making it a useful method for cytometric analysis of biological samples, including, for example, whole blood, bone marrow aspirates, peritoneal fluids, and other red blood cell containing samples. The invention also provides a method to recover or “unmask” epitopes on intracellular antigens that have been made inaccessible by the cross linking fixative necessary to fix the sample. Significantly, the methods of the invention allow preservation and analysis of phospho-epitope levels in biological samples taken directly from patients to determine disease-specific characteristics.

    摘要翻译： 本发明涉及一种用于制备用于测量蛋白质表位的生物样品的方法，其允许基于捕获表位的瞬时激活状态的能力来保留细胞内蛋白质表位和检测信号转导途径。 本发明提供的方法允许快速固定含有红细胞的生物样品，以确保信号转导分子和其他细胞内蛋白质表位的表位保持活跃状态​​。 本发明的方法还允许红细胞溶解，从而使其成为生物样品的细胞分析的有用方法，包括例如全血，骨髓抽吸物，腹膜液和其它含红细胞的样品。 本发明还提供了一种在细胞内抗原上恢复或“揭开”表位的方法，这些表位已经被固定样品所必需的交联固定剂制成。 显着地，本发明的方法允许保存和分析直接从患者获取的生物样品中的磷酸化表位水平，以确定疾病特异性特征。

    Composite profiles of cell antigens and target signal transduction proteins for analysis and clinical management of hematologic cancers
    8.
    发明申请
    Composite profiles of cell antigens and target signal transduction proteins for analysis and clinical management of hematologic cancers 审中-公开
    细胞抗原和靶信号转导蛋白的复合谱，用于分析和临床管理血液癌

    公开(公告)号：US20070105165A1

    公开(公告)日：2007-05-10

    申请号：US11267948

    申请日：2005-11-04

    申请人： Charles Goolsby ,  T. Shankey ,  David Hedley ,  James Jacobberger ,  Stanley Shackney

    发明人： Charles Goolsby ,  T. Shankey ,  David Hedley ,  James Jacobberger ,  Stanley Shackney

    IPC分类号： G01N33/574

    CPC分类号： G01N33/57426 ,  G01N33/5052 ,  G01N2800/52

    摘要： The present invention is directed to methods for establishing a composite marker profile for a sample derived from an individual suspected having a neoplastic condition. A composite marker profile of the invention allows for identification of prognostically and therapeutically relevant subgroups of neoplastic conditions and prediction of the clinical course of an individual. The methods of the invention provide tools useful in choosing a therapy for an individual afflicted with a neoplastic condition, including methods for assigning a risk group, methods of predicting an increased risk of relapse, methods of predicting an increased risk of developing secondary complications, methods of choosing a therapy for an individual, methods of determining the efficacy of a therapy in an individual, and methods of determining the prognosis for an individual. In particular, the method of the present invention discloses a method for establishing a composite marker profile that can serve as a prognostic indicator to predict whether the course of a neoplastic condition in a individual will be aggressive or indolent, thereby aiding the clinician in managing the patient and evaluating the modality of treatment to be used. In particular embodiments disclosed herein, the methods of the invention are directed to establishing a composite marker profile for a leukemia selected from the group consisting of Chronic Lymphocytic Leukemia (CLL), Acute Myelogenous Leukemia (AML), Chronic Myelogenous Leukemia (CML), and Acute Lymphocytic Leukemia (ALL).

    摘要翻译： 本发明涉及用于建立来自疑似具有肿瘤状况的个体的样品的复合标记物谱的方法。 本发明的复合标记物分布允许鉴定预后和治疗相关的肿瘤病症亚组和个体临床病程的预测。 本发明的方法提供了用于选择患有肿瘤病症的个体的治疗的工具，包括分配风险组的方法，预测复发风险增加的方法，预测发生继发性并发症的风险增加的方法，方法 选择个体治疗的方法，确定个体治疗功效的方法以及确定个体预后的方法。 特别地，本发明的方法公开了一种建立复合标记物谱的方法，该方法可用作预后指标以预测个体肿瘤状况的进程是否是侵略性或惰性的，由此帮助临床医师管理 患者和评估使用的治疗方式。 在本文公开的特定实施方案中，本发明的方法涉及建立选自慢性淋巴细胞性白血病（CLL），急性骨髓性白血病（AML），慢性骨髓性白血病（CML）和 急性淋巴细胞性白血病（ALL）。

    Whole blood preparation for cytometric analysis of cell signaling pathways
    9.
    发明申请
    Whole blood preparation for cytometric analysis of cell signaling pathways 有权
    全血制备细胞信号传导途径的细胞分析

    公开(公告)号：US20060046272A1

    公开(公告)日：2006-03-02

    申请号：US10928570

    申请日：2004-08-27

    申请人： Sue Chow ,  David Hedley ,  T. Shankey ,  Patricia Grom

    发明人： Sue Chow ,  David Hedley ,  T. Shankey ,  Patricia Grom

    IPC分类号： G01N33/567 ,  G01N33/53

    CPC分类号： G01N33/80 ,  Y10T436/107497 ,  Y10T436/108331 ,  Y10T436/25 ,  Y10T436/25125 ,  Y10T436/2525 ,  Y10T436/25375

    摘要： This invention is directed to a method for preparation of a biological sample for measurement of protein epitopes that allows for the preservation of intracellular protein epitopes and detection of signal transduction pathways based on the ability to capture transient activation states of the epitopes. The method provided by the invention allows for the rapid fixation of biological samples containing red blood cells, to ensure that epitopes of signal transduction molecules and other intracellular protein epitopes are preserved in the active state. The method of the invention further allows for lysis of red blood cells, thereby making it a useful method for cytometric analysis of biological samples, including, for example, whole blood, bone marrow aspirates, peritoneal fluids, and other red blood cell containing samples. The invention also provides a method to recover or “unmask” epitopes on intracellular antigens that have been made inaccessible by the cross linking fixative necessary to fix the sample. Significantly, the methods of the invention allow preservation and analysis of phospho-epitope levels in biological samples taken directly from patients to determine disease-specific characteristics.

    摘要翻译： 本发明涉及一种用于制备用于测量蛋白质表位的生物样品的方法，其允许基于捕获表位的瞬时激活状态的能力来保留细胞内蛋白质表位和检测信号转导途径。 本发明提供的方法允许快速固定含有红细胞的生物样品，以确保信号转导分子和其他细胞内蛋白质表位的表位保持活跃状态​​。 本发明的方法还允许红细胞溶解，从而使其成为生物样品的细胞分析的有用方法，包括例如全血，骨髓抽吸物，腹膜液和其它含红细胞的样品。 本发明还提供了一种在细胞内抗原上恢复或“揭开”表位的方法，这些表位已经被固定样品所必需的交联固定剂制成。 显着地，本发明的方法允许保存和分析直接从患者获取的生物样品中的磷酸化表位水平，以确定疾病特异性特征。

    Composite Profiles of Cell Antigens and Target Signal Transduction Proteins for Analysis and Clinical Management of Hematologic Cancers
    10.
    发明申请
    Composite Profiles of Cell Antigens and Target Signal Transduction Proteins for Analysis and Clinical Management of Hematologic Cancers 审中-公开
    复合型细胞抗原和目标信号转导蛋白用于分析和临床管理的血液癌

    公开(公告)号：US20100261204A1

    公开(公告)日：2010-10-14

    申请号：US12696702

    申请日：2010-01-29

    申请人： Charles GOOLSBY ,  T. Vincent Shankey ,  David Hedley ,  James Jacobberger ,  Stanley Shackney

    发明人： Charles GOOLSBY ,  T. Vincent Shankey ,  David Hedley ,  James Jacobberger ,  Stanley Shackney

    IPC分类号： G01N33/574

    CPC分类号： G01N33/57426 ,  G01N33/5052 ,  G01N2800/52

    摘要： The present invention is directed to methods for establishing a composite marker profile for a sample derived from an individual suspected having a neoplastic condition. A composite marker profile of the invention allows for identification of prognostically and therapeutically relevant subgroups of neoplastic conditions and prediction of the clinical course of an individual. The methods of the invention provide tools useful in choosing a therapy for an individual afflicted with a neoplastic condition, including methods for assigning a risk group, methods of predicting an increased risk of relapse, methods of predicting an increased risk of developing secondary complications, methods of choosing a therapy for an individual, methods of determining the efficacy of a therapy in an individual, and methods of determining the prognosis for an individual. In particular, the method of the present invention discloses a method for establishing a composite marker profile that can serve as a prognostic indicator to predict whether the course of a neoplastic condition in a individual will be aggressive or indolent, thereby aiding the clinician in managing the patient and evaluating the modality of treatment to be used. In particular embodiments disclosed herein, the methods of the invention are directed to establishing a composite marker profile for a leukemia selected from the group consisting of Chronic Lymphocytic Leukemia (CLL), Acute Myelogenous Leukemia (AML), Chronic Myelogenous Leukemia (CML), and Acute Lymphocytic Leukemia (ALL).

    摘要翻译： 本发明涉及用于建立来自疑似具有肿瘤状况的个体的样品的复合标记物谱的方法。 本发明的复合标记物分布允许鉴定预后和治疗相关的肿瘤病症亚组和个体临床病程的预测。 本发明的方法提供了用于选择患有肿瘤病症的个体的治疗的工具，包括分配风险组的方法，预测复发风险增加的方法，预测发生继发性并发症的风险增加的方法，方法 选择个体治疗的方法，确定个体治疗功效的方法以及确定个体预后的方法。 特别地，本发明的方法公开了一种建立复合标记物谱的方法，该方法可用作预后指标以预测个体肿瘤状况的进程是否是侵略性或惰性的，由此帮助临床医师管理 患者和评估使用的治疗方式。 在本文公开的特定实施方案中，本发明的方法涉及建立选自慢性淋巴细胞性白血病（CLL），急性骨髓性白血病（AML），慢性骨髓性白血病（CML）和 急性淋巴细胞性白血病（ALL）。