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公开(公告)号：US5672499A
公开(公告)日：1997-09-30
申请号：US478920
申请日：1995-06-07
IPC分类号： C07K16/28 , C12N5/0797 , C12Q1/02 , C12N15/85
CPC分类号： C07K16/2863 , C12N5/0623 , C12N2500/20 , C12N2500/25 , C12N2500/35 , C12N2500/38 , C12N2500/46 , C12N2500/80 , C12N2500/90 , C12N2501/02 , C12N2501/11 , C12N2501/385 , C12N2501/39 , C12N2501/392 , C12N2501/395 , C12N2501/86 , C12N2510/00 , C12N2533/32 , C12N2533/52 , C12N2533/56
摘要： The invention includes mammalian multipotent neural stem cells and their progeny and methods for the isolation and clonal propagation of such cells. At the clonal level the stem cells are capable of self regeneration and asymmetrical division. Lineage restriction is demonstrated within developing clones which are sensitive to the local environment. The invention also includes such cells which are transfected with foreign nucleic acid, e.g., to produce an immortalized neural stem cell. The invention further includes transplantation assays which allow for the identification of mammalian multipotent neural stem cells from various tissues and methods for transplanting mammalian neural stem cells and/or neural or glial progenitors into mammals. A novel method for detecting antibodies to neural cell surface markers is disclosed as well as a monoclonal antibody to mouse LNGFR.
摘要翻译： 本发明包括哺乳动物多能神经干细胞及其后代和用于分离和克隆繁殖这些细胞的方法。 在克隆级别，干细胞能够进行自我再生和不对称分裂。 在对本地环境敏感的开发克隆中证明了谱系限制。 本发明还包括用外源核酸转染的细胞，例如产生永生化的神经干细胞。 本发明还包括允许从各种组织鉴定哺乳动物多能神经干细胞的移植测定法和用于将哺乳动物神经干细胞和/或神经或神经胶质祖细胞移植到哺乳动物中的方法。 公开了一种用于检测神经细胞表面标志物的抗体的新方法以及小鼠LNGFR的单克隆抗体。
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公开(公告)号：US5589376A
公开(公告)日：1996-12-31
申请号：US290228
申请日：1994-08-15
IPC分类号： C12N5/0797 , C12N5/00
CPC分类号： C12N5/0623 , C12N2500/20 , C12N2500/25 , C12N2500/35 , C12N2500/38 , C12N2500/46 , C12N2500/80 , C12N2500/90 , C12N2501/02 , C12N2501/11 , C12N2501/385 , C12N2501/39 , C12N2501/392 , C12N2501/395 , C12N2501/86 , C12N2510/00 , C12N2533/32 , C12N2533/52 , C12N2533/56
摘要： The invention includes methods for the isolation and clonal propagation of mammalian neural crest stem cells and isolated cellular compositions comprising the same. The methods employ a novel separation and culturing regimen and bioassays for establishing the generation of neural crest stem cell derivatives. These methods result in the production of non-transformed neural crest stem cells and their progeny. The invention demonstrates, at the clonal level, the self regeneration and asymmetrical division of mammalian neural crest stem cells for the first time in feeder cell-independent cultures. Lineage restriction is demonstrated within a developing clone and is shown to be sensitive to the local environment. Neural crest stem cells cultured on a mixed substrate of poly-D-lysine and fibronectin generate PNS neurons and glia, but on fibronectin alone the stem cells generate PNS glia but not neurons. The neurogenic potential of the stem cells, while not expressed, is maintained over time on fibronectin. The invention further includes transplantation assays which allow for the identification of mammalian neural crest stem cells from various tissues. It also includes methods for transplanting mammalian neural crest stem cells and/or neural or glial progenitors into mammals.
摘要翻译： 本发明包括用于哺乳动物神经嵴干细胞的分离和克隆繁殖的方法和包含其的分离的细胞组合物。 该方法采用新型分离培养方案和生物测定法建立神经嵴干细胞衍生物的产生。 这些方法导致未转化的神经嵴干细胞及其后代的产生。 本发明在克隆水平上表现出哺乳动物神经嵴干细胞在饲养细胞非依赖性培养物中的自我再生和不对称分裂。 谱系限制在开发的克隆中证明，并且显示出对局部环境敏感。 在聚-D-赖氨酸和纤连蛋白混合基质上培养的神经嵴干细胞产生PNS神经元和神经胶质，但是单独的纤连蛋白上，干细胞产生PNS神经胶质，而不产生神经元。 干细胞的神经源性潜力虽然未被表达，但随着时间的推移在纤连蛋白上保持。 本发明还包括允许从各种组织鉴定哺乳动物神经嵴干细胞的移植测定。 它还包括将哺乳动物神经嵴干细胞和/或神经或胶质祖细胞移植到哺乳动物中的方法。
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公开(公告)号：US5928947A
公开(公告)日：1999-07-27
申请号：US483142
申请日：1995-06-07
IPC分类号： C07K16/28 , C12N5/0797 , C12N15/00 , A61K35/30 , C12N15/85
CPC分类号： C07K16/2863 , C12N5/0623 , C12N2500/20 , C12N2500/25 , C12N2500/35 , C12N2500/38 , C12N2500/46 , C12N2500/80 , C12N2500/90 , C12N2501/02 , C12N2501/11 , C12N2501/385 , C12N2501/39 , C12N2501/392 , C12N2501/395 , C12N2501/86 , C12N2510/00 , C12N2533/32 , C12N2533/52 , C12N2533/56
摘要： The invention includes mammalian multipotent neural stem cells and their progeny and methods for the isolation and clonal propagation of such cells. At the clonal level the stem cells are capable of self regeneration and asymmetrical division. Lineage restriction is demonstrated within developing clones which are sensitive to the local environment. The invention also includes such cells which are transfected with foreign nucleic acid, e.g., to produce an immortalized neural stem cell. The invention further includes transplantation assays which allow for the identification of mammalian multipotent neural stem cells from various tissues and methods for transplanting mammalian neural stem cells and/or neural or glial progenitors into mammals. A novel method for detecting antibodies to neural cell surface markers is disclosed as well as a monoclonal antibody to mouse LNGFR.
摘要翻译： 本发明包括哺乳动物多能神经干细胞及其后代和用于分离和克隆繁殖这些细胞的方法。 在克隆级别，干细胞能够进行自我再生和不对称分裂。 在对本地环境敏感的开发克隆中证明了谱系限制。 本发明还包括用外源核酸转染的细胞，例如产生永生化的神经干细胞。 本发明还包括允许从各种组织鉴定哺乳动物多能神经干细胞的移植测定法和用于将哺乳动物神经干细胞和/或神经或神经胶质祖细胞移植到哺乳动物中的方法。 公开了一种用于检测神经细胞表面标志物的抗体的新方法以及小鼠LNGFR的单克隆抗体。
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公开(公告)号：US5693482A
公开(公告)日：1997-12-02
申请号：US474506
申请日：1995-06-07
IPC分类号： C07K16/28 , C12N5/0797 , C12Q1/02 , C12N15/85
CPC分类号： C07K16/2863 , C12N5/0623 , C12N2500/20 , C12N2500/25 , C12N2500/35 , C12N2500/38 , C12N2500/46 , C12N2500/80 , C12N2500/90 , C12N2501/02 , C12N2501/11 , C12N2501/385 , C12N2501/39 , C12N2501/392 , C12N2501/395 , C12N2501/86 , C12N2510/00 , C12N2533/32 , C12N2533/52 , C12N2533/56
摘要： The invention includes mammalian multipotent neural stem cells and their progeny and methods for the isolation and clonal propagation of such cells. At the clonal level the stem cells are capable of self regeneration and asymmetrical division. Lineage restriction is demonstrated within developing clones which are sensitive to the local environment. The invention also includes such cells which are transfected with foreign nucleic acid, e.g., to produce an immortalized neural stem cell. The invention further includes transplantation assays which allow for the identification of mammalian multipotent neural stem cells from various tissues and methods for transplanting mammalian neural stem cells and/or neural or glial progenitors into mammals. A novel method for detecting antibodies to neural cell surface markers is disclosed as well as a monoclonal antibody to mouse LNGFR.
摘要翻译： 本发明包括哺乳动物多能神经干细胞及其后代和用于分离和克隆繁殖这些细胞的方法。 在克隆级别，干细胞能够自我再生和不对称分裂。 在对当地环境敏感的开发克隆中证明了谱系限制。 本发明还包括用外源核酸转染的细胞，例如产生永生化的神经干细胞。 本发明还包括允许从各种组织鉴定哺乳动物多能神经干细胞的移植测定法和用于将哺乳动物神经干细胞和/或神经或神经胶质祖细胞移植到哺乳动物中的方法。 公开了一种用于检测神经细胞表面标志物的抗体的新方法以及小鼠LNGFR的单克隆抗体。
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公开(公告)号：US5849553A
公开(公告)日：1998-12-15
申请号：US485612
申请日：1995-06-07
IPC分类号： C07K16/28 , C12N5/0797 , C12N15/85 , C12N15/09
CPC分类号： C07K16/2863 , C12N5/0623 , C12N2500/20 , C12N2500/25 , C12N2500/35 , C12N2500/38 , C12N2500/46 , C12N2500/80 , C12N2500/90 , C12N2501/02 , C12N2501/11 , C12N2501/385 , C12N2501/39 , C12N2501/392 , C12N2501/395 , C12N2501/86 , C12N2510/00 , C12N2533/32 , C12N2533/52 , C12N2533/56
摘要： The invention includes mammalian multipotent neural stem cells and their progeny and methods for the isolation and clonal propagation of such cells. At the clonal level the stem cells are capable of self regeneration and asymmetrical division. Lineage restriction is demonstrated within developing clones which are sensitive to the local environment. The invention also includes such cells which are transfected with foreign nucleic acid, e.g., to produce an immortalized neural stem cell, and immortalized cell lines which are capable of subsequent disimmortalization. The invention further includes transplantation assays which allow for the identification of mammalian multipotent neural stem cells from various tissues and methods for transplanting mammalian neural stem cells and/or neural or glial progenitors into mammals. A novel method for detecting antibodies to neural cell surface markers is disclosed as well as a monoclonal antibody to mouse LNGFR.
摘要翻译： 本发明包括哺乳动物多能神经干细胞及其后代和用于分离和克隆繁殖这些细胞的方法。 在克隆级别，干细胞能够自我再生和不对称分裂。 在对本地环境敏感的开发克隆中证明了谱系限制。 本发明还包括用外源核酸转染的细胞，例如产生永生化神经干细胞，以及能够随后进行破碎的永生化细胞系。 本发明还包括允许从各种组织鉴定哺乳动物多能神经干细胞的移植测定法和用于将哺乳动物神经干细胞和/或神经或神经胶质祖细胞移植到哺乳动物中的方法。 公开了一种用于检测神经细胞表面标志物的抗体的新方法以及小鼠LNGFR的单克隆抗体。
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6.发明授权In vitro method for obtaining an isolated population of mammalian neural crest stem cells 失效用于获得哺乳动物神经嵴干细胞群体的体外方法
公开(公告)号：US5824489A
公开(公告)日：1998-10-20
申请号：US290229
申请日：1994-08-15
IPC分类号： A01K67/027 , C12N5/0797 , C12N5/10 , C12N15/09 , C12P21/08 , C12R1/91 , G01N33/577 , C12N5/00
CPC分类号： C12N5/0623 , C12N2500/20 , C12N2500/25 , C12N2500/35 , C12N2500/38 , C12N2500/46 , C12N2500/80 , C12N2500/90 , C12N2501/02 , C12N2501/11 , C12N2501/385 , C12N2501/39 , C12N2501/392 , C12N2501/395 , C12N2501/86 , C12N2510/00 , C12N2533/32 , C12N2533/52 , C12N2533/56
摘要： The invention includes methods for the isolation and clonal propagation of mammalian neural stem cells. The methods employ a novel separation and culturing regimen and bioassays for establishing the generation of neural stem cell derivatives. These methods result in the production of non-transformed neural stem cells and their progeny. The invention demonstrates, at the clonal level, the self regeneration and asymmetrical division of mammalian neural stem cells for the first time in feeder cell-independent cultures. Lineage restriction is demonstrated within a developing clone and is shown to be sensitive to the local environment. Multipotent neural stem cells cultured on a mixed substrate of poly-D-lysine and fibronectin generate PNS neurons and glia, but on fibronectin alone the stem cells generate PNS glia but not neurons. The neurogenic potential of the stem cells, while not expressed, is maintained over time on fibronectin. The invention further includes transplantation assays which allow for the identification of mammalian neural stem cells from various tissues. It also includes methods for transplanting mammalian neural stem cells and/or neural or glial progenitors into mammals.
摘要翻译： 本发明包括哺乳动物神经干细胞分离和克隆繁殖的方法。 该方法采用新型分离培养方案和生物测定法，用于建立神经干细胞衍生物的产生。 这些方法导致未转化的神经干细胞及其后代的产生。 本发明在克隆水平上证明哺乳动物神经干细胞在饲养细胞非依赖性培养物中的自我再生和不对称分裂。 谱系限制在开发的克隆中证明，并且显示出对局部环境敏感。 在多聚赖氨酸和纤连蛋白的混合底物上培养的多能神经干细胞产生PNS神经元和神经胶质细胞，但是单独的纤连蛋白上，干细胞产生PNS神经胶质，而不是神经元。 干细胞的神经源性潜力虽然未被表达，但随着时间的推移在纤连蛋白上保持。 本发明还包括允许从各种组织鉴定哺乳动物神经干细胞的移植测定。 它还包括将哺乳动物神经干细胞和/或神经或神经胶质祖细胞移植到哺乳动物中的方法。
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公开(公告)号：US5654183A
公开(公告)日：1997-08-05
申请号：US188286
申请日：1994-01-28
IPC分类号： C12N5/0797 , C12N15/85 , C12N15/00
CPC分类号： C12N5/0623 , C12N2500/20 , C12N2500/25 , C12N2500/35 , C12N2500/38 , C12N2500/46 , C12N2500/80 , C12N2500/90 , C12N2501/02 , C12N2501/11 , C12N2501/385 , C12N2501/39 , C12N2501/392 , C12N2501/395 , C12N2501/86 , C12N2510/00 , C12N2533/32 , C12N2533/52 , C12N2533/56
摘要： The invention includes mammalian multipotent neural stem cells and their progeny and methods for the isolation and clonal propagation of such cells. At the clonal level the stem cells are capable of self regeneration and asymmetrical division. Lineage restriction is demonstrated within developing clones which are sensitive to the local environment. The invention also includes such cells which are transfected with foreign nucleic acid, e.g., to produce an immortalized neural stem cell. The invention further includes transplantation assays which allow for the identification of mammalian multipotent neural stem cells from various tissues and methods for transplanting mammalian neural stem cells and/or neural or glial progenitors into mammals. A novel method for detecting antibodies to neural cell surface markers is disclosed as well as a monoclonal antibody to mouse LNGFR.
摘要翻译： 本发明包括哺乳动物多能神经干细胞及其后代和用于分离和克隆繁殖这些细胞的方法。 在克隆级别，干细胞能够自我再生和不对称分裂。 在对本地环境敏感的开发克隆中证明了谱系限制。 本发明还包括用外源核酸转染的细胞，例如产生永生化的神经干细胞。 本发明还包括允许从各种组织鉴定哺乳动物多能神经干细胞的移植测定法和用于将哺乳动物神经干细胞和/或神经或神经胶质祖细胞移植到哺乳动物中的方法。 公开了一种用于检测神经细胞表面标志物的抗体的新方法以及小鼠LNGFR的单克隆抗体。
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公开(公告)号：US10071310B2
公开(公告)日：2018-09-11
申请号：US14805552
申请日：2015-07-22
IPC分类号： G10L17/00 , G10L15/00 , H04L29/06 , A63F13/424 , A63F13/215 , A63F13/79 , A63F13/54
CPC分类号： A63F13/424 , A63F13/12 , A63F13/215 , A63F13/54 , A63F13/79 , A63F2300/1081 , A63F2300/50 , A63F2300/6072 , A63F2300/6081 , G10L15/00 , G10L17/00 , H04L65/00
摘要： A thick-game client device includes all of the game data processing on-board while a thin-game client device includes only a small portion of game data processing such as to render visual displays while the majority of the game data processing is network based. A thick-voice client devices includes all of the voice processing on-board while a thin-voice client device includes only a small portion of the voice processing such as to generate acoustic parameter data discussed below while the remainder of the voice processing occurs at a voice services node. It will be appreciated that a client device may be thick with respect to both game processing and voice processing, may be thick with respect to one type of processing and thin with respect to the other, or may be thin with respect to both types of processing.
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公开(公告)号：US20130091591A1
公开(公告)日：2013-04-11
申请号：US13597626
申请日：2012-08-29
申请人： Hai U. Wang , Zhoufeng Chen , David J. Anderson
发明人： Hai U. Wang , Zhoufeng Chen , David J. Anderson
IPC分类号： A61K49/00 , A61K39/395 , A61K47/48 , A01K67/027
CPC分类号： C12N15/8509 , A01K67/0275 , A01K67/0276 , A01K2217/075 , A01K2227/105 , A01K2267/03 , A01K2267/0375 , A61K39/3955 , A61K49/00 , A61K49/0002 , A61K49/0008 , C07K14/705 , C07K16/2866 , C07K16/30 , C07K2317/76 , C12N5/0691 , C12N2503/02 , G01N33/573 , G01N33/6863 , G01N2333/52
摘要： Arterial and venous endothelial cells are molecularly distinct from the earliest stages of angiogenesis. This distinction is revealed by expression on arterial cells of a transmembrane ligand, called EphrinB2 whose receptor EphB4 is expressed on venous cells. Targeted disruption of the EphrinB2 gene prevents the remodeling of veins from a capillary plexus into properly branched structures. Moreover, it also disrupts the remodeling of arteries, suggesting that reciprocal interactions between pre-specified arterial and venous endothelial cells are necessary for angiogenesis. This distinction can be used to advantage in methods to alter angiogenesis, methods to assess the effect of drugs on artery cells and vein cells, and methods to identify and isolate artery cells and vein cells, for example.
摘要翻译： 动脉和静脉内皮细胞与血管生成的最早阶段分子不同。 通过在受体EphB4在静脉细胞上表达的称为EphrinB2的跨膜配体的动脉细胞上的表达来揭示这种区别。 EphrinB2基因的靶向破坏阻止了从毛细血管丛将静脉重构成适当的分支结构。 此外，它还破坏动脉重塑，这表明预先规定的动脉和静脉内皮细胞之间的相互作用对于血管发生是必需的。 这种区别可用于改变血管生成的方法，评估药物对动脉细胞和静脉细胞的作用的方法，以及鉴定和分离动脉细胞和静脉细胞的方法。
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公开(公告)号：US08332046B2
公开(公告)日：2012-12-11
申请号：US12848828
申请日：2010-08-02
IPC分类号： A61N1/05
CPC分类号： A61B5/04001 , A61N1/0534 , A61N1/0539 , Y10T29/49204
摘要： A neural interface system including an electrode array and a carrier that supports the electrode array, in which the electrode array includes a substrate rolled into a three-dimensional shape, a plurality of conductive traces patterned on the substrate and adapted to transmit electrical signals, and a plurality of elliptically shaped, externally facing electrode sites coupled to the plurality of conductive traces that electrically communicate with their surroundings. The plurality of electrodes are arranged in a triangular lattice circumferentially around and axially along the carrier, and the substrate includes an edge that extends axially along the carrier and is constrained between a first axial row portion of the plurality of electrode sites and a second axial row portion of the plurality of electrode sites adjacent to the first axial row portion.
摘要翻译： 一种神经接口系统，包括支撑电极阵列的电极阵列和载体，其中电极阵列包括滚动成三维形状的基底，在基底上图案化并适于透射电信号的多个导电迹线，以及 耦合到与其周围环境电连通的多个导电迹线的多个椭圆形的外部对置的电极位置。 所述多个电极沿着所述载体围绕并且轴向地布置成三角形晶格，并且所述基板包括沿着所述载体轴向延伸并且被约束在所述多个电极位置的第一轴向行部分和第二轴向行 多个电极位置的与第一轴向行部分相邻的部分。
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