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    Activation of regulatory T cells by alpha-melanocyte stimulating hormone
    2.
    发明申请
    Activation of regulatory T cells by alpha-melanocyte stimulating hormone 审中-公开
    通过α-黑素细胞刺激激素激活调节性T细胞

    公开(公告)号：US20060127400A1

    公开(公告)日：2006-06-15

    申请号：US11346938

    申请日：2006-02-03

    申请人： Andrew Taylor ,  Tomomi Nishida

    发明人： Andrew Taylor ,  Tomomi Nishida

    IPC分类号： A61K39/395 ,  C12P21/04 ,  C12N5/06

    CPC分类号： A61K38/34 ,  A61K39/0008 ,  A61K2035/122 ,  C12N5/0636 ,  C12N2500/90 ,  C12N2501/15 ,  C12N2501/515 ,  C12N2501/86 ,  C12N2510/00

    摘要： The invention encompasses a method of down-regulating a T cell-mediated immune response, through activation or T cell receptor (TCR) stimulation of antigen-primed T cells in the presence of alpha-melanocyte stimulating hormone (α-MSH), which may be optionally enhanced by adding transforming growth factor-β2 (TGF-β2) approximately 4-6 hours after the start of the primed T cells' exposure to α-MSH. Activation of the primed T cells may be mediated by presentation of the specific antigen to the primed T cells, or by an anti-TCR antibody or a T cell mitogen. As a result of the α-MSH treatment modulating the T cell activation, antigen-specific, regulatory, CD4+/CD25+ T cells are generated that produce transforming growth factor-β (TGF-β) and can non-specifically down-regulate Th1-mediated inflammatory activities. The method may be used to down-regulate or suppress an autoimmune condition or a graft rejection in a transplant patient. The invention also encompasses a kit for generating regulatory T cell comprising a specific antigen, α-MSH, and optionally, TGF-β2 and/or a T cell culture medium. Also provided are gene therapy treatments for suppressing an autoimmune or graft rejection response, or for re-establishing autotolerance, by introducing genetic material (e.g. nucleic acid) for expressing α-MSH or a receptor-binding portion thereof, into a localized tissue site.

    摘要翻译： 本发明包括通过在α-黑素细胞刺激激素（α-MSH）的存在下通过激活或T细胞受体（TCR）刺激抗原引发的T细胞来降低T细胞介导的免疫应答的方法，其可以 任选地通过在引发的T细胞暴露于α-MSH开始后约4-6小时加入转化生长因子-β2（TGF-β2）来增强。 引发的T细胞的活化可以通过将特异性抗原呈递给引发的T细胞或通过抗TCR抗体或T细胞丝裂原来介导。 由于α-MSH处理调节T细胞活化的结果，产生产生转化生长因子-β（TGF-β）的抗原特异性，调节性CD 4 + / CD 25 + T细胞，并且可以非特异性地下调 调节Th1介导的炎症活动。 该方法可用于下调或抑制移植患者中的自身免疫病症或移植排斥反应。 本发明还包括用于产生包含特异性抗原α-MSH和任选的TGF-β2和/或T细胞培养基的调节性T细胞的试剂盒。 还提供了通过将用于表达α-MSH或其受体结合部分的遗传物质（例如核酸）引入局部组织部位来抑制自身免疫或移植排斥反应或重建自身耐受性的基因治疗治疗。

    FUNCTIONAL MYELINATION OF NEURONS
    4.
    发明申请
    FUNCTIONAL MYELINATION OF NEURONS 审中-公开
    神经元的功能性神经元

    公开(公告)号：US20160060597A1

    公开(公告)日：2016-03-03

    申请号：US14838345

    申请日：2015-08-27

    申请人： The University of Maryland, Baltimore ,  The United States of America as represented by the Department of Veterans Affairs

    发明人： Thomas Hornyak ,  Sandeep Joshi

    IPC分类号： C12N5/0797

    CPC分类号： C12N5/0623 ,  A61K35/36 ,  C12N5/0622 ,  C12N5/0626 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/33 ,  C12N2501/365 ,  C12N2501/86 ,  C12N2502/081 ,  C12N2506/091

    摘要： Hair follicle bulge region/LLP region CD34(+) MeSCs can be isolated from mammalian skin bearing hair follicles. These cells are multipotent and retain the ability to differentiate into cells of neural crest lineage, including glia-like cells that express the glial marker Gfap, and are able to express myelin basic protein, and to remyelinate naked (unmyelinated or demyelinated) neuronal processes with a functional, dense myelin sheath. These cells of neural crest lineage can be used to produce a dense myelin sheath on neurons which lack myelin due to genetic defect, trauma, toxin, infection, or disease process. Therefore, embodiments of the invention provide methods for preparing such cells, the cells themselves and compositions containing the cells, as well as methods for using the cells.

    摘要翻译： 毛囊突起区域/ LLP区域CD34（+）MeSC可以从携带哺乳动物的毛囊分离。 这些细胞是多能的，并且保留分化成神经嵴谱系细胞的能力，包括表达神经胶质标记Gfap的神经胶质样细胞，并且能够表达髓磷脂碱性蛋白，并且用裸髓（无髓鞘或脱髓鞘）神经元过程进行髓鞘化 功能密集的髓鞘。 神经嵴谱系的这些细胞可用于在遗传缺陷，创伤，毒素，感染或疾病过程中缺乏髓磷脂的神经元上产生致密的髓鞘。 因此，本发明的实施方案提供了制备这种细胞的方法，细胞本身和含有细胞的组合物，以及使用细胞的方法。

    Mammalian multipotent neural stem cells
    9.
    发明授权
    Mammalian multipotent neural stem cells 失效
    哺乳动物多能神经干细胞

    公开(公告)号：US5928947A

    公开(公告)日：1999-07-27

    申请号：US483142

    申请日：1995-06-07

    申请人： David J. Anderson ,  Derek L. Stemple

    发明人： David J. Anderson ,  Derek L. Stemple

    IPC分类号： C07K16/28 ,  C12N5/0797 ,  C12N15/00 ,  A61K35/30 ,  C12N15/85

    CPC分类号： C07K16/2863 ,  C12N5/0623 ,  C12N2500/20 ,  C12N2500/25 ,  C12N2500/35 ,  C12N2500/38 ,  C12N2500/46 ,  C12N2500/80 ,  C12N2500/90 ,  C12N2501/02 ,  C12N2501/11 ,  C12N2501/385 ,  C12N2501/39 ,  C12N2501/392 ,  C12N2501/395 ,  C12N2501/86 ,  C12N2510/00 ,  C12N2533/32 ,  C12N2533/52 ,  C12N2533/56

    摘要： The invention includes mammalian multipotent neural stem cells and their progeny and methods for the isolation and clonal propagation of such cells. At the clonal level the stem cells are capable of self regeneration and asymmetrical division. Lineage restriction is demonstrated within developing clones which are sensitive to the local environment. The invention also includes such cells which are transfected with foreign nucleic acid, e.g., to produce an immortalized neural stem cell. The invention further includes transplantation assays which allow for the identification of mammalian multipotent neural stem cells from various tissues and methods for transplanting mammalian neural stem cells and/or neural or glial progenitors into mammals. A novel method for detecting antibodies to neural cell surface markers is disclosed as well as a monoclonal antibody to mouse LNGFR.

    摘要翻译： 本发明包括哺乳动物多能神经干细胞及其后代和用于分离和克隆繁殖这些细胞的方法。 在克隆级别，干细胞能够进行自我再生和不对称分裂。 在对本地环境敏感的开发克隆中证明了谱系限制。 本发明还包括用外源核酸转染的细胞，例如产生永生化的神经干细胞。 本发明还包括允许从各种组织鉴定哺乳动物多能神经干细胞的移植测定法和用于将哺乳动物神经干细胞和/或神经或神经胶质祖细胞移植到哺乳动物中的方法。 公开了一种用于检测神经细胞表面标志物的抗体的新方法以及小鼠LNGFR的单克隆抗体。

    Neural chest stem cell assay
    10.
    发明授权
    Neural chest stem cell assay 失效
    神经胸干细胞测定

    公开(公告)号：US5693482A

    公开(公告)日：1997-12-02

    申请号：US474506

    申请日：1995-06-07

    申请人： David J. Anderson ,  Derek L. Stemple

    发明人： David J. Anderson ,  Derek L. Stemple

    IPC分类号： C07K16/28 ,  C12N5/0797 ,  C12Q1/02 ,  C12N15/85

    CPC分类号： C07K16/2863 ,  C12N5/0623 ,  C12N2500/20 ,  C12N2500/25 ,  C12N2500/35 ,  C12N2500/38 ,  C12N2500/46 ,  C12N2500/80 ,  C12N2500/90 ,  C12N2501/02 ,  C12N2501/11 ,  C12N2501/385 ,  C12N2501/39 ,  C12N2501/392 ,  C12N2501/395 ,  C12N2501/86 ,  C12N2510/00 ,  C12N2533/32 ,  C12N2533/52 ,  C12N2533/56

    摘要： The invention includes mammalian multipotent neural stem cells and their progeny and methods for the isolation and clonal propagation of such cells. At the clonal level the stem cells are capable of self regeneration and asymmetrical division. Lineage restriction is demonstrated within developing clones which are sensitive to the local environment. The invention also includes such cells which are transfected with foreign nucleic acid, e.g., to produce an immortalized neural stem cell. The invention further includes transplantation assays which allow for the identification of mammalian multipotent neural stem cells from various tissues and methods for transplanting mammalian neural stem cells and/or neural or glial progenitors into mammals. A novel method for detecting antibodies to neural cell surface markers is disclosed as well as a monoclonal antibody to mouse LNGFR.

    摘要翻译： 本发明包括哺乳动物多能神经干细胞及其后代和用于分离和克隆繁殖这些细胞的方法。 在克隆级别，干细胞能够自我再生和不对称分裂。 在对当地环境敏感的开发克隆中证明了谱系限制。 本发明还包括用外源核酸转染的细胞，例如产生永生化的神经干细胞。 本发明还包括允许从各种组织鉴定哺乳动物多能神经干细胞的移植测定法和用于将哺乳动物神经干细胞和/或神经或神经胶质祖细胞移植到哺乳动物中的方法。 公开了一种用于检测神经细胞表面标志物的抗体的新方法以及小鼠LNGFR的单克隆抗体。