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    Preparations, compositions, and methods for nucleic acid sequencing
    9.
    发明授权
    Preparations, compositions, and methods for nucleic acid sequencing 有权
    用于核酸测序的制备物，组合物和方法

    公开(公告)号：US08795961B2

    公开(公告)日：2014-08-05

    申请号：US12553478

    申请日：2009-09-03

    申请人： David R. Rank ,  Paul Peluso

    发明人： David R. Rank ,  Paul Peluso

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6869 ,  C12Q2565/537 ,  C12Q2535/122 ,  C12Q2535/101

    摘要： Preparations, compositions and methods of sequencing nucleic acids are provided. The preparations, methods and compositions employ multiple priming sites on a target nucleic acid and typically utilize single molecule sequencing processes to identify sequence portions of the target which are then assembled to obtain the sequence of the target.

    摘要翻译： 提供了测序核酸的制备，组合物和方法。 制备，方法和组合物在靶核酸上采用多个引发位点，并且通常利用单分子测序过程来鉴定靶的序列部分，然后将其组装以获得靶序列。

    Polymerase enzymes and reagents for enhanced nucleic acid sequencing
    10.
    发明授权
    Polymerase enzymes and reagents for enhanced nucleic acid sequencing 有权
    用于增强核酸测序的聚合酶和试剂

    公开(公告)号：US08343746B2

    公开(公告)日：2013-01-01

    申请号：US11977160

    申请日：2007-10-22

    申请人： David R. Rank ,  Arek Bibillo ,  Paul Peluso

    发明人： David R. Rank ,  Arek Bibillo ,  Paul Peluso

    IPC分类号： C12N9/12 ,  C12P19/34

    CPC分类号： C12N9/1252 ,  C12P19/34

    摘要： Compositions that include DNA polymerases having increased residence times for nucleotide analogues, particularly modified recombinant Φ29-type DNA polymerases with such increased residence times, are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA amplification are also provided. Compositions including α-thiophosphate nucleotide analogues with four or more phosphate groups are described, as are methods for determining the sequence of nucleic acid molecules using such analogues.

    摘要翻译： 提供包含具有增加的核苷酸类似物停留时间的DNA聚合酶的组合物，特别是具有这种增加的停留时间的经修饰的重组Φ29型DNA聚合酶。 还提供了在测序和DNA扩增中制备聚合酶和使用聚合酶的方法。 还描述了包含具有四个或更多个磷酸基团的α-硫代磷酸核苷酸类似物的组合物，以及使用这种类似物确定核酸分子的序列的方法。