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    Determination of light absorption pathlength in a vertical-beam photometer
    3.
    发明申请
    Determination of light absorption pathlength in a vertical-beam photometer 审中-公开

    公开(公告)号：US20060238764A1

    公开(公告)日：2006-10-26

    申请号：US11302993

    申请日：2005-12-13

    申请人： Dean Hafeman ,  Calvin Chow

    发明人： Dean Hafeman ,  Calvin Chow

    IPC分类号： G01N21/00 ,  G01J3/427

    CPC分类号： G01J3/08 ,  G01J3/1804 ,  G01N21/253 ,  G01N21/31 ,  G01N21/314 ,  G01N21/6452

    摘要： Disclosed are photometric methods and devices for determining optical pathlength of liquid samples containing analytes dissolved or suspended in a solvent. The methods and devices rely on determining a relationship between the light absorption properties of the solvent and the optical pathlength of liquid samples containing the solvent. This relationship is used to establish the optical pathlength for samples containing an unknown concentration of analyte but having similar solvent composition. Further disclosed are methods and devices for determining the concentration of analyte in such samples where both the optical pathlength and the concentration of analyte are unknown. The methods and devices rely on separately determining, at different wavelengths of light, light absorption by the solvent and light absorption by the analyte. Light absorption by the analyte, together with the optical pathlength so determined, is used to calculate the concentration of the analyte. Devices for carrying out the methods particularly advantageously include vertical-beam photometers containing samples disposed within the wells of multi-assay plates, wherein the photometer is able to monitor light absorption of each sample at multiple wavelengths, including in the visible or UV-visible region of the spectrum, as well as in the near-infrared region of the electromagnetic spectrum. Novel photometer devices are described which automatically determine the concentration of analytes in such multi-assay plates directly without employing a standard curve.

    Optical system for a scanning fluorometer
    6.
    发明授权
    Optical system for a scanning fluorometer 有权
    用于扫描荧光计的光学系统

    公开(公告)号：US06236456B1

    公开(公告)日：2001-05-22

    申请号：US09337623

    申请日：1999-06-21

    申请人： Robert Giebeler ,  David G. Ogle ,  Roger Kaye ,  Dean Hafeman

    发明人： Robert Giebeler ,  David G. Ogle ,  Roger Kaye ,  Dean Hafeman

    IPC分类号： G01N2164

    CPC分类号： G01N21/6452 ,  G01N2021/6421 ,  G01N2021/6471

    摘要： A method and apparatus for determining the fluorescence, luminescence, or absorption of a sample is provided. The sample may either be contained within a cuvette or within one or more sample wells within a multi-assay plate. A combination of a broadband source, a monochromator, and a series of optical filters are used to tune the excitation wavelength to a predetermined value within a relatively wide wavelength band. A similar optical configuration is used to tune the detection wavelength. In one aspect, multiple optical fibers are coupled to the excitation source subassembly, thus allowing the system to be quickly converted from one optical configuration to another. For example, the source can be used to illuminate either the top or the bottom of a sample well within a multi-assay plate or to illuminate a single cuvette cell. Similarly, multiple optical fibers are coupled to the detector subassembly. In another aspect, the excitation light and the detected sample emissions pass to and from an optical head assembly via a pair of optical fibers. The optical head assembly is scanned across one axis of the sample multi-assay plate. The multi-assay plate is mounted to a carriage assembly that scans the plate along a second axis orthogonal to the first axis. In another aspect, an optical scanning head assembly is used that includes mirrored optics for coupling the excitation source to the sample and the emitted light to the detector.

    摘要翻译： 提供了用于确定样品的荧光，发光或吸收的方法和装置。 样品可以包含在比色杯中或多个测定板内的一个或多个样品孔内。 使用宽带源，单色器和一系列滤光器的组合来将激发波长调谐到相对宽的波长带内的预定值。 使用类似的光学配置来调谐检测波长。 在一个方面，多个光纤耦合到激发源子组件，从而允许系统从一个光学配置快速转换到另一个。 例如，源可以用于在多分析板内照亮样品孔的顶部或底部或照亮单个比色杯池。 类似地，多个光纤耦合到检测器子组件。 另一方面，激发光和检测到的样品发射经由一对光纤经过光学头组件和/或从光学头组件传出。 沿着样品多分析板的一个轴扫描光学头组件。 多测定板被安装到托架组件，其沿着与第一轴正交的第二轴扫描板。 在另一方面，使用光学扫描头组件，其包括镜像光学器件，用于将激发源耦合到样品，并将发射的光耦合到检测器。

    Optimization systems in a scanning fluorometer
    7.
    发明授权
    Optimization systems in a scanning fluorometer 有权
    扫描荧光计中的优化系统

    公开(公告)号：US06232608B1

    公开(公告)日：2001-05-15

    申请号：US09274796

    申请日：1999-03-23

    申请人： Robert Giebeler ,  Thomas J. Smith ,  Steven McNerney ,  Dean Hafeman ,  Gillian Humphries

    发明人： Robert Giebeler ,  Thomas J. Smith ,  Steven McNerney ,  Dean Hafeman ,  Gillian Humphries

    IPC分类号： G01N2164

    CPC分类号： G01N21/6452 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6471

    摘要： A method and apparatus for determining the fluorescence, luminescence, or absorption of a sample is provided. The sample may either be contained within a cuvette or within one or more sample wells within a multi-assay plate. A combination of a broadband source, monochromator, and a series of optical filters are used to tune the excitation wavelength to a predetermined value within a relatively wide wavelength band. A similar configuration is used to tune the detection wavelength. In one aspect, multiple optical filters are coupled to the excitation source subassembly, thus allowing the system to be quickly converted from one optical configuration to another. In another aspect, the excitation light and the detected sample emissions pass to and from an optical head assembly via a pair of optical fibers. In another aspect, an optical scanning head assembly is used that includes mirrored optics for coupling the excitation source to the sample and the emitted light to the detector. In another aspect, time tags are recorded for samples contained within a multi-assay plate to monitor compositional time dependent properties or to ensure the accurate comparison of individual samples.

    摘要翻译： 提供了用于确定样品的荧光，发光或吸收的方法和装置。 样品可以包含在比色杯中或多个测定板内的一个或多个样品孔内。 使用宽带源，单色器和一系列光学滤波器的组合来将激发波长调谐到相对宽的波长带内的预定值。 类似的配置用于调整检测波长。 在一个方面，多个光学滤波器耦合到激励源子组件，从而允许系统从一个光学配置快速转换到另一个。 另一方面，激发光和检测到的样品发射经由一对光纤经过光学头组件和/或从光学头组件传出。 在另一方面，使用光学扫描头组件，其包括镜像光学器件，用于将激发源耦合到样品，并将发射的光耦合到检测器。 在另一方面，记录包含在多测定板中的样品的时间标签以监测组合时间依赖性质或确保各个样品的准确比较。

    Methods for Prevention of Surface Adsorption of Biological Materials to Capillary Walls in Microchannels
    9.
    发明申请
    Methods for Prevention of Surface Adsorption of Biological Materials to Capillary Walls in Microchannels 有权
    生物材料表面吸附微通道毛细管壁的方法

    公开(公告)号：US20070246076A1

    公开(公告)日：2007-10-25

    申请号：US11772744

    申请日：2007-07-02

    申请人： Dean Hafeman ,  Aileen Zhou

    发明人： Dean Hafeman ,  Aileen Zhou

    IPC分类号： B08B9/00

    CPC分类号： B08B17/00 ,  B01L3/502753 ,  B01L3/502761 ,  B01L3/561 ,  B01L2200/0647 ,  B01L2200/141 ,  B01L2300/161 ,  B01L2300/163 ,  B01L2300/165 ,  B01L2400/0415 ,  B01L2400/0487 ,  Y10S977/904 ,  Y10S977/92

    摘要： Methods for reducing surface adsorption of biological materials to the walls of microfluidic conduits in microscale devices are provided. In an example of the methods, one or more colloidal-size particles, such as colloidal silica particles, are flowed in a fluid within the microfluidic conduit in the presence of one or more adherent biological materials (such as one or more proteins, cells, carbohydrates, nucleic acids, lipids and the like) to adsorb to the materials and prevent them from binding to the capillary walls of the microfluidic conduit. Other adsorption inhibition agents such as detergents and nonaqueous solvents can be used alone or in combination with colloidal particles to reduce surface adsorption in microfluidic conduits.

    摘要翻译： 提供了在微型装置中减少生物材料对微流体导管壁的表面吸附的方法。 在所述方法的一个实例中，一种或多种胶体尺寸颗粒，例如胶体二氧化硅颗粒，在一种或多种粘附的生物材料（例如一种或多种蛋白质，细胞， 碳水化合物，核酸，脂质等）吸附到材料上并防止它们结合到微流体导管的毛细管壁。 其它吸附抑制剂如洗涤剂和非水溶剂可以单独使用或与胶体颗粒组合使用以减少微流体导管中的表面吸附。

    Capture and release assay system and method
    10.
    发明申请
    Capture and release assay system and method 审中-公开
    捕获和释放测定系统和方法

    公开(公告)号：US20060211055A1

    公开(公告)日：2006-09-21

    申请号：US11440615

    申请日：2006-05-24

    申请人： Dean Hafeman ,  H. Wada ,  Anton Simeonov

    发明人： Dean Hafeman ,  H. Wada ,  Anton Simeonov

    IPC分类号： G01N33/53 ,  C12Q1/68

    CPC分类号： G01N33/54306

    摘要： A method for detecting one or more components of interest in a fluid-borne sample in a microchannel of a microfluidic device includes flowing the fluid-borne sample, in which the one or more components of interest are bound to a first labeled component binding moiety to form a first labeled complex, through a binding channel region of the microchannel. The binding channel region includes a second component-binding moiety reversibly bound to a wall surface of the binding channel region. At least a portion of the one or more components of interest is bound to the second component-binding moiety to form a second labeled complex. The second labeled complex is released from the binding channel region and flowed through a detection channel region of the microchannel, where the second labeled complex is detected.

    摘要翻译： 用于检测微流体装置的微通道中的流体样品中的一种或多种感兴趣组分的方法包括使流体传播的样品流动，其中所述一种或多种感兴趣的组分与第一标记的组分结合部分结合， 通过微通道的结合通道区形成第一标记的复合物。 结合通道区域包括可逆结合到结合通道区域的壁表面的第二组分结合部分。 感兴趣的一种或多种组分的至少一部分与第二组分结合部分结合以形成第二标记复合物。 第二标记的复合物从结合通道区域释放并流过微通道的检测通道区域，其中检测到第二标记复合物。