公开(公告)号：US4070495A

公开(公告)日：1978-01-24

申请号：US672572

申请日：1976-03-31

申请人： Dieter Berger ,  Werner Guthlein ,  Wolfgang Werner ,  Peter Rieckmann

发明人： Dieter Berger ,  Werner Guthlein ,  Wolfgang Werner ,  Peter Rieckmann

IPC分类号： G01N33/48 ,  G01N1/30 ,  G01N33/16 ,  G01N21/60 ,  G01N31/22

CPC分类号： G01N1/30

摘要： Microscope slide for blood investigation having a coating of highly purified methylene blue N and highly purified cresyl violet acetate in a weight ratio of from 1:1.5 to 1:5.

摘要翻译： 用于血液研究的显微镜载玻片，其重量比为1:1.5至1:5，具有高度纯化的亚甲基蓝N和高度纯化的甲酚紫乙酸酯的涂层。

公开(公告)号：US4260777A

公开(公告)日：1981-04-07

申请号：US740462

申请日：1976-11-10

申请人： Walter Rittersdorf ,  Werner Guthlein ,  Wolfgang Werner ,  Hanz-Georg Rey ,  Peter Rieckmann

发明人： Walter Rittersdorf ,  Werner Guthlein ,  Wolfgang Werner ,  Hanz-Georg Rey ,  Peter Rieckmann

IPC分类号： C07D327/04 ,  C07D307/88 ,  C09B11/00 ,  C09B11/08 ,  G01N31/00 ,  G01N33/52 ,  G01N33/68

CPC分类号： C09B11/08 ,  G01N33/6839

摘要： Protein in body fluids, e.g., urine, is detected by contacting a sample of the body fluid with a diagnostic agent comprising an absorbent carrier impregnated with a pH indicator of the octahalosulfophthalein group, a buffer, and at least one linear or branched chain polypropylene glycol immiscible with water; the resulting test papers are highly sensitive to protein without being subject to interfering effects by nitrogen-containing compounds.

摘要翻译： 通过使体液样品与包含浸渍有八氢硫代沙丁胺基的pH指示剂的缓冲剂载体的诊断剂，缓冲液和至少一种直链或支链聚丙二醇来检测体液（例如尿液）中的蛋白质 与水不混溶; 所得到的试纸对蛋白质高度敏感，不受含氮化合物的干扰作用。

公开(公告)号：US4013416A

公开(公告)日：1977-03-22

申请号：US661687

申请日：1976-02-26

申请人： Walter Rittersdorf ,  Werner Guthlein ,  Wolfgang Werner ,  Hanz-Georg Rey ,  Peter Rieckmann

发明人： Walter Rittersdorf ,  Werner Guthlein ,  Wolfgang Werner ,  Hanz-Georg Rey ,  Peter Rieckmann

IPC分类号： C07D327/04 ,  C07D307/88 ,  C09B11/00 ,  C09B11/08 ,  G01N31/00 ,  G01N33/52 ,  G01N33/68 ,  G01N31/22 ,  G01N33/16

CPC分类号： C09B11/08 ,  G01N33/6839

摘要： Protein in body fluids, e.g., urine, is detected by contacting a sample of the body fluid with a diagnostic agent comprising an absorbent carrier impregnated with a pH indicator of the octahalosulfophthalein group, a buffer, and at least one linear or branched chain polypropylene glycol immiscible with water; the resulting test papers are highly sensitive to protein without being subject to interfering effects by nitrogen-containing compounds.

摘要翻译： 通过使体液样品与包含浸渍有八氢硫代沙丁胺基的pH指示剂的缓冲剂载体的诊断剂，缓冲液和至少一种直链或支链聚丙二醇来检测体液（例如尿液）中的蛋白质 与水不混溶; 所得到的试纸对蛋白质高度敏感，不受含氮化合物的干扰作用。

公开(公告)号：US4278763A

公开(公告)日：1981-07-14

申请号：US99180

申请日：1979-11-30

申请人： Dieter Berger ,  Franz Braun ,  Werner Guthlein ,  Manfred Kuhr ,  Wolfgang Werner

发明人： Dieter Berger ,  Franz Braun ,  Werner Guthlein ,  Manfred Kuhr ,  Wolfgang Werner

IPC分类号： C07D209/36 ,  C07D209/12 ,  C07D333/64 ,  C07K1/113 ,  C07K5/04 ,  C07K7/06 ,  C12Q1/37 ,  G01N33/48 ,  G01N33/50 ,  C12Q1/38

CPC分类号： C12Q1/37 ,  C12Q2334/50

摘要： The present invention provides a diagnostic agent for the detection of proteolytic enzymes, comprising an absorbent carrier, a film layer, a powder mixture, a lyophilisate, a solution or a reagent tablet, containing at least one chromogen and an appropriate buffer substance, wherein the chromogen used is an indoxyl- and/or thioindoxyl-amino acid ester and/or peptide ester of the general formula: ##STR1## wherein R.sub.1, R.sub.2, R.sub.3 and R.sub.4, which can be the same or different, are hydrogen or halogen atoms, lower alkyl, lower alkoxy, aryl, aralkyl, aralkoxy, hydroxyl, carboxy, carboxy lower alkoxy, aralkoxycarbonyl, aralkyloxycarbonyl lower alkoxy, nitro or lower acylamino radicals, whereby two adjacent substituents can also represent an optionally halogenated fused benzene ring, X is a sulphur atom or an imino group optionally substituted by a lower alkyl, aryl, aralkyl or acyl radical, A is an amino acid or peptide residue and B is a nitrogen protective group conventional in peptide chemistry or derived therefrom.The present invention also provides new compounds of the above-given general formula and processes for the preparation thereof.Furthermore, the present invention is concerned with the use of the compounds of the above-given general formula for the production of diagnostic agents for the detection of proteolytic enzymes.

摘要翻译： 本发明提供了用于检测蛋白水解酶的诊断剂，其包含含有至少一种色原体和适当的缓冲物质的吸收剂载体，膜层，粉末混合物，冻干物，溶液或试剂片剂，其中 使用的色原是通式为：其中R 1，R 2，R 3和R 4可以相同或不同，为氢或卤素原子的通式如下的吲哚氧基和/或硫代吲哚氧基 - 氨基酸酯和/或肽酯： 低级烷基，低级烷氧基，芳基，芳烷基，芳烷氧基，羟基，羧基，羧基低级烷氧基，芳烷氧基羰基，芳烷氧基羰基低级烷氧基，硝基或低级酰基氨基，其中两个相邻的取代基也可以表示任选卤代的稠合苯环，X是硫 原子或任选被低级烷基，芳基，芳烷基或酰基取代的亚氨基，A是氨基酸或肽残基，B是肽化学中常规的氮保护基或 从其衍生。 本发明还提供了上述给出的通式的新化合物及其制备方法。 此外，本发明涉及上述给出的通式的化合物用于产生用于检测蛋白水解酶的诊断剂的用途。

公开(公告)号：US4331760A

公开(公告)日：1982-05-25

申请号：US42393

申请日：1979-05-25

申请人： Dieter Berger ,  Franz Braun ,  Gunter Frey ,  Werner Guthlein ,  Wolfgang Werner

发明人： Dieter Berger ,  Franz Braun ,  Gunter Frey ,  Werner Guthlein ,  Wolfgang Werner

IPC分类号： G01N33/49 ,  C07D327/04 ,  C09B11/00 ,  C09B11/06 ,  G01N33/50 ,  C12Q1/44 ,  G01N33/48

CPC分类号： G01N33/5094 ,  C07D327/04 ,  Y02P20/55

摘要： Diagnostic agent for the detection of leukocytes in body fluids, comprising an absorbent carrier which is impregnated with a chromogen and a buffer substance, wherein the chromogen is a sulfonphthalein ester of the formula ##STR1## wherein R.sub.1 is a carboxylic acid residue optionally substituted by halogen or lower alkoxy; or is an amino acid or peptide residue having a nitrogen protective group;R.sub.2 is a halogen atom or a lower alkyl radical; andR.sub.3 and R.sub.4 are individually selected from hydrogen and halogen.Certain of the chromogen are provided as novel compounds.

摘要翻译： 用于检测体液中白细胞的诊断剂，其包含用色素沉积物和缓冲物质浸渍的吸收剂载体，其中所述色原是式IMAMA的磺酞类，其中R1是任选被卤素取代的羧酸残基 或低级烷氧基; 或具有氮保护基的氨基酸或肽残基; R2是卤素原子或低级烷基; 并且R 3和R 4分别选自氢和卤素。 某些色原提供为新化合物。

公开(公告)号：US3988208A

公开(公告)日：1976-10-26

申请号：US559273

申请日：1975-03-17

申请人： Wolfgang Werner ,  Peter Vogel ,  Hugo Tiedemann ,  Werner Guthlein

发明人： Wolfgang Werner ,  Peter Vogel ,  Hugo Tiedemann ,  Werner Guthlein

IPC分类号： C07D209/88 ,  C12Q1/28 ,  C12Q1/54 ,  C12K1/04

CPC分类号： C12Q1/54 ,  C07D209/88 ,  C12Q1/28 ,  C12Q2326/00 ,  C12Q2326/14

摘要： Novel 3-aminocarbazole compounds of the formula: ##SPC1##WhereinX is hydrogen or halogen,Y is oxygen or represents two hydrogen atoms; andR.sub.1 and R.sub.2, which can be the same or different, are hydrogen, lower alkyl or hydroxyalkyl or amino; andN is 1 or 2, with the proviso that when n is2, X, Y, R.sub.1 and R.sub.2 cannot all be hydrogen atoms, as well as the salts thereof with inorganic and organic acids; are outstandingly effective indicators for the determination of glucose and provide stable compositions for such glucose determination.

摘要翻译： 新型3-氨基咔唑化合物，具有下式：

公开(公告)号：US4219336A

公开(公告)日：1980-08-26

申请号：US892359

申请日：1978-03-31

申请人： Werner Guthlein ,  Hans Wielinger ,  Walter Rittersdorf ,  Wolfgang Werner

发明人： Werner Guthlein ,  Hans Wielinger ,  Walter Rittersdorf ,  Wolfgang Werner

IPC分类号： G01N33/50 ,  A61K36/18 ,  C07G99/00 ,  C12Q1/28 ,  G01N33/72 ,  G01N33/16

CPC分类号： C12Q1/28 ,  G01N33/725 ,  C12Q2326/00 ,  Y10S436/904

摘要： A diagnostic agent for the detection of occult blood in feces comprising hydrogen peroxide and, as a chromogen, guaiaconic acid A, optionally with a stabilizer. The invention provides, as a novel substance, guaiaconic acid A having a specific extinction E.sub.1 cm.sup.1% at 600 nm of at least 200, determined by the reaction with peroxidase and hydrogen peroxide, having infra-red bands at1600 cm.sup.-1 (m);1505 cm.sup.-1 (v.s.);1260 cm.sup.-1 (s;b);1115 cm.sup.-1 (m);1025 cm.sup.-1 (m); and1200 cm.sup.-1 (s;b)and having an R.sub.F value of 0.45 (toluene/dioxan/glacial acetic acid; 90:25:10 v/v/v).

摘要翻译： 用于检测粪便中的潜血的诊断剂，其包含过氧化氢和任选地与稳定剂作为色原，愈创木酸A。 本发明提供作为新物质，通过与过氧化物酶和过氧化氢的反应确定在600nm处具有600nm以上的具有特定消光度E1cm1％的愈创木酸A至少为200，在1600cm -1处具有红外线

公开(公告)号：US4385114A

公开(公告)日：1983-05-24

申请号：US236487

申请日：1981-02-23

申请人： Werner Guthlein ,  Walter Rittersdorf ,  Hugo Tiedemann ,  Peter Vogel ,  Wolfgang Werner

发明人： Werner Guthlein ,  Walter Rittersdorf ,  Hugo Tiedemann ,  Peter Vogel ,  Wolfgang Werner

IPC分类号： C07C211/50 ,  C07C67/00 ,  C07C209/00 ,  C07C209/54 ,  C12Q1/00 ,  C12Q1/26 ,  C12Q1/28 ,  G01N21/78 ,  G01N31/00 ,  G01N31/22 ,  G01N33/50 ,  C12Q1/60 ,  C12Q1/62

CPC分类号： C12Q1/26 ,  C12Q1/28 ,  G01N31/228 ,  C12Q2326/10 ,  C12Q2326/12 ,  Y10S435/805 ,  Y10S436/81 ,  Y10T436/144444 ,  Y10T436/206664

摘要： The detection and determination of (a) hydrogen peroxide or of a substance which reacts with the formation of hydrogen peroxide or (b) of peroxidase or a peroxidatively active substance, is carried out by contacting an unknown sample with a chromogen and, in case (a), with a peroxidatively active substance or in case (b) with hydrogen peroxide or a substance which reacts with the formation of hydrogen peroxide, and evaluating the coloration produced by the reaction, wherein, as the chromogen, there is used at least one 3,3',5,5'-tetraalkylbenzidine compound of the formula: ##STR1## wherein R.sub.1, R.sub.2, R.sub.3 and R.sub.4, which can be the same or different, are straight-chained or branched alkyl radicals containing up to 6 and preferably up to 4 carbon atoms.

摘要翻译： （a）过氧化氢或与过氧化氢或过氧化物酶或过氧化物活性物质的（b）反应形成的物质的检测和测定是通过将​​未知样品与色原体接触进行的，如果（ a），具有过氧化活性物质或在（b）过氧化氢或与形成过氧化氢反应的物质的情况下，以及评价由反应产生的着色，其中作为色原，使用至少一种 具有下式的3,3'，5,5'-四烷基联苯胺化合物：其中R 1，R 2，R 3和R 4可以相同或不同，是含有至多6个的直链或支链烷基，优选 最多4个碳原子。

公开(公告)号：US3975398A

公开(公告)日：1976-08-17

申请号：US487338

申请日：1974-07-10

申请人： Wolfgang Werner ,  Peter Vogel ,  Hugo Tiedemann ,  Werner Guthlein

发明人： Wolfgang Werner ,  Peter Vogel ,  Hugo Tiedemann ,  Werner Guthlein

IPC分类号： C07D209/88 ,  C12Q1/28 ,  C12Q1/54 ,  G01N31/00 ,  G01N31/22 ,  G01N33/50 ,  G01N33/66 ,  G01N33/68

CPC分类号： C12Q1/28 ,  C07D209/88 ,  C12Q1/54 ,  C12Q2326/00 ,  C12Q2326/14 ,  Y10S436/81

摘要： Novel 3-aminocarbazole compounds of the formula: ##SPC1##WhereinX is hydrogen or halogen,Y is oxygen or represents two hydrogen atoms; andR.sub.1 and R.sub.2, which can be the same or different, are hydrogen, lower alkyl or hydroxyalkyl or amino; andn is 1 or 2, with the proviso that when n is 2, X, Y, R.sub.1 and R.sub.2 cannot all be hydrogen atoms,As well as the salts thereof with inorganic and organic acids; are outstandingly effective indicators for the determination of glucose and provide stable compositions for such glucose determination.

摘要翻译： 新型3-氨基咔唑化合物，具有下式：

公开(公告)号：US4245096A

公开(公告)日：1981-01-13

申请号：US34720

申请日：1979-04-30

申请人： Anselm Rothe ,  Werner Guthlein ,  Walter Rittersdorf ,  Wolfgang Werner

发明人： Anselm Rothe ,  Werner Guthlein ,  Walter Rittersdorf ,  Wolfgang Werner

IPC分类号： C07D213/04 ,  C07D213/20 ,  C07D213/24 ,  C07D213/26 ,  C07D215/10 ,  C07D217/10 ,  C09B11/00 ,  C09B11/26 ,  G01N31/22 ,  G01N33/62 ,  G01N33/84 ,  C07D215/12

CPC分类号： C07D213/20 ,  C07D213/24 ,  C07D215/10 ,  C07D217/10

摘要： Bis-(2,4-dinitrophenyl)-methylpyridinium compounds useful as a pH indicator or for the detection of ammonia having the formula: ##STR1## wherein one of R.sub.1 and R.sub.2 is a bis-(2,4-dinitrophenyl)-methyl radical and the other is a lower alkyl radical;R.sub.2 can also be hydrogen atom or trifluoromethyl;R.sub.3 and R.sub.4 are hydrogen or, together with the carbon atoms to which they are attached, represent a benzene ring; andX.sup..crclbar. is an appropriate acid anionor said compounds are betaines.

摘要翻译： 可用作pH指示剂或用于检测具有下式的氨的双（2,4-二硝基苯基） - 甲基吡啶鎓化合物：其中R1和R2之一是双 - （2,4-二硝基苯基） - 甲基 另一个是低级烷基; R2也可以是氢原子或三氟甲基; R3和R4是氢或与它们相连的碳原子一起代表苯环; 并且X（ - ）是适当的酸性阴离子或所述化合物是甜菜碱。