公开(公告)号：US20180072998A1

公开(公告)日：2018-03-15

申请号：US15702893

申请日：2017-09-13

申请人： E I DU PONT DE NEMOURS AND COMPANY

发明人： YOUGEN LI ,  SUSAN MARIE HENNESSEY ,  SLAVKO KRALJ ,  MARK S. PAYNE ,  VELI ALKAN ,  QIONG CHENG ,  ROBERT DICOSIMO ,  ELLEN D SEMKE ,  JARED B. PARKER ,  RICHARD R. BOTT

IPC分类号： C12N9/10 ,  C12P19/04 ,  C12P19/18

CPC分类号： C12N9/1051 ,  C08B37/0009 ,  C12P19/04 ,  C12P19/18

摘要： Disclosed herein are glucosyltransferases with modified amino acid sequences. Such engineered enzymes exhibit improved alpha-glucan product yields and/or lower leucrose yields, for example. Further disclosed are reactions and methods in which engineered glucosyltransferases are used to produce alpha-glucan.

公开(公告)号：US20130102670A1

公开(公告)日：2013-04-25

申请号：US13657603

申请日：2012-10-22

申请人： E. I. Du Pont De Nemours And Company

发明人： ROBERT DICOSIMO ,  MARK SCOTT PAYNE ,  JOHN EDWARD GAVAGAN

IPC分类号： C12N9/18 ,  C12N1/19 ,  C12N1/21 ,  C12N15/63 ,  D21C9/16 ,  C12N15/55 ,  A01N37/16 ,  A01P1/00 ,  C11D3/395 ,  D06L3/02 ,  C12N1/15 ,  C12P7/24

CPC分类号： A01N37/16 ,  C11D1/667 ,  C11D3/2093 ,  C11D3/221 ,  C11D3/226 ,  C11D3/38636 ,  C11D3/3869 ,  C11D3/3945 ,  C11D7/266 ,  C12N9/18 ,  C12P7/40 ,  D06L4/15 ,  D06L4/40 ,  D21C9/16 ,  Y10T428/13

摘要： An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.

公开(公告)号：US20190322963A1

公开(公告)日：2019-10-24

申请号：US16416318

申请日：2019-05-20

申请人： E I DU PONT DE NEMOURS AND COMPANY

发明人： ROBERT DICOSIMO ,  Qiong Cheng ,  Rakesh Nambiar ,  Jayme L. Paullin ,  Mark S. Payne ,  Jahnavi Chandra Prasad ,  Zheng You

IPC分类号： C11D3/22 ,  C11D3/00

摘要： An enzymatically produced α-glucan oligomer/polymer compositions is provided. The enzymatically produced α-glucan oligomer/polymers can be derivatized into α-glucan ether compounds. The α-glucan oligomers/polymers and the corresponding α-glucan ethers are cellulose and/or protease resistant, making them suitable for use in fabric care and laundry care applications. Methods for the production and use of the present compositions are also provided.

公开(公告)号：US20130102671A1

公开(公告)日：2013-04-25

申请号：US13657607

申请日：2012-10-22

申请人： E. I. Du Pont De Nemours And Company

发明人： ROBERT DICOSIMO ,  MARK SCOTT PAYNE ,  JOHN EDWARD GAVAGAN

IPC分类号： C12N9/18 ,  C12N1/15 ,  C12N1/19 ,  C12P7/24 ,  C12N15/80 ,  C12N15/81 ,  C12N15/75 ,  C12N15/76 ,  C12N15/77 ,  C12N15/78 ,  C12N15/70 ,  C12N15/55 ,  A01N37/16 ,  A01P1/00 ,  C11D3/395 ,  D06L3/02 ,  D21C9/16 ,  B65D69/00 ,  C12N1/21

CPC分类号： D21C9/16 ,  C11D3/38636 ,  C12N9/18 ,  C12P7/40 ,  C12P7/54 ,  C12Y301/01072 ,  D06L4/10 ,  D06L4/40 ,  D06M16/00 ,  D06M16/003 ,  D06M16/006 ,  D21C5/005

摘要： An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.

公开(公告)号：US20190048376A1

公开(公告)日：2019-02-14

申请号：US15988349

申请日：2018-05-24

申请人： E I DU PONT DE NEMOURS AND COMPANY

发明人： KEVIN D. NAGY ,  ERWIN F. COLUMBUS-HAGO ,  JAYARAMA K. SHETTY ,  SUSAN MARIE HENNESSEY ,  ROBERT DICOSIMO ,  LING HUA ,  RODRIGO RAMIREZ ,  ZHONGMEI TANG ,  ZHEYONG YU

IPC分类号： C12P19/14 ,  C12P19/16 ,  C12P7/06 ,  C12P19/18 ,  C12P19/02 ,  C12P7/14 ,  C12P19/04

摘要： A method is disclosed for hydrolyzing an alpha-1,3 or alpha-1,6 glucosyl-glucose linkage in a saccharide (disaccharide or oligosaccharide). This method comprises contacting the saccharide with an alpha-glucosidase enzyme such as transglucosidase under suitable conditions, during which contacting step the enzyme hydrolyzes at least one alpha-1,3 or alpha-1,6 glucosyl-glucose linkage of the saccharide. This method is useful for reducing the amount of oligosaccharides in a filtrate isolated from a glucan synthesis reaction, for example.

公开(公告)号：US20130102664A1

公开(公告)日：2013-04-25

申请号：US13657575

申请日：2012-10-22

申请人： E. I. du Pont de Nemours and Company

发明人： ROBERT DICOSIMO ,  MARK PAYNE SCOTT ,  JOHN EDWARD GAVAGAN

IPC分类号： C12N9/18 ,  C12N15/63 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  D21C9/16 ,  C12M1/40 ,  A01N37/16 ,  C11D3/395 ,  D06L3/02 ,  A01P1/00 ,  C12N15/55 ,  C12P7/40

CPC分类号： C12N9/18 ,  C11D3/386 ,  C11D3/38654 ,  C11D3/38681 ,  C11D3/3945 ,  C12P7/40 ,  C12Y301/01072 ,  D06L1/04 ,  D06L4/15 ,  D06M16/00 ,  D21C9/166

摘要： An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.

公开(公告)号：US20180273999A1

公开(公告)日：2018-09-27

申请号：US15976246

申请日：2018-05-10

申请人： E I DU PONT DE NEMOURS AND COMPANY

发明人： KEVIN D NAGY ,  ERWIN F. COLUMBUS-HAGO ,  JAYARAMA K. SHETTY ,  SUSAN MARIE HENNESSEY ,  ROBERT DICOSIMO ,  LING HUA ,  RODRIGO RAMIREZ ,  ZHONGMEI TANG ,  ZHEYONG YU

IPC分类号： C12P19/14 ,  C12P19/16 ,  C12P7/06 ,  C12P19/18 ,  C12P19/04 ,  C12P7/14 ,  C12P19/02

CPC分类号： C12P19/14 ,  C12P7/06 ,  C12P7/14 ,  C12P19/02 ,  C12P19/04 ,  C12P19/16 ,  C12P19/18 ,  Y02E50/17

摘要： A method is disclosed for hydrolyzing an alpha-1,5 glucosyl-fructose linkage in a saccharide (disaccharide or oligosaccharide) such as leucrose. This method comprises contacting the saccharide with an alpha-glucosidase enzyme such as transglucosidase or glucoamylase under suitable conditions, during which contacting step the enzyme hydrolyzes at least one alpha-1,5 glucosyl-fructose linkage of the saccharide. This method is useful for reducing the amount of leucrose in a filtrate isolated from a glucan synthesis reaction, for example.

公开(公告)号：US20130102666A1

公开(公告)日：2013-04-25

申请号：US13657582

申请日：2012-10-22

申请人： E. I. Du Pont De Nemours And Company

发明人： ROBERT DICOSIMO ,  MARK SCOTT PAYNE ,  JOHN EDWARD GAVAGAN

IPC分类号： C12N9/18 ,  C12N15/63 ,  C12N1/15 ,  C12N1/19 ,  D21C9/16 ,  C12P7/40 ,  C12M1/40 ,  A01N37/16 ,  C11D3/395 ,  D06L3/02 ,  C12N15/55 ,  C12N1/21

CPC分类号： A01N37/16 ,  C11D1/667 ,  C11D3/2093 ,  C11D3/221 ,  C11D3/226 ,  C11D3/38636 ,  C11D3/3869 ,  C11D3/3945 ,  C11D7/266 ,  C12N9/18 ,  C12P7/40 ,  C12Y301/01072 ,  D06L4/15 ,  D06L4/40 ,  D21C9/16

摘要： An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.

公开(公告)号：US20180355072A1

公开(公告)日：2018-12-13

申请号：US16111514

申请日：2018-08-24

申请人： E I DU PONT DE NEMOURS AND COMPANY

发明人： RAKESH NAMBIAR ,  RONG GUAN ,  QIONG CHENG ,  ROBERT DICOSIMO ,  JAYME L. PAULLIN ,  YUANFENG LIANG ,  CHARLES R. POWLEY ,  YEFIM BRUN

IPC分类号： C08B37/02 ,  C08L5/02 ,  C12P19/08 ,  C11D3/22

CPC分类号： C08B37/0021 ,  C08L5/02 ,  C11D3/222 ,  C12P19/08

摘要： Compositions are disclosed herein comprising dextran that comprises (i) 87-93 wt % glucose linked at positions 1 and 6; (ii) 0.1-1.2 wt % glucose linked at positions 1 and 3; (iii) 0.1-0.7 wt % glucose linked at positions 1 and 4; (iv) 7.7-8.6 wt % glucose linked at positions 1, 3 and 6; and (v) about 0.4-1.7 wt % glucose linked at (a) positions 1, 2 and 6, or (b) positions 1, 4 and 6. Aqueous forms of this composition have enhanced viscosity profiles. Further disclosed are methods of using compositions comprising dextran, such as increasing the viscosity of an aqueous composition. Enzymatic reactions for producing dextran are also disclosed.

公开(公告)号：US20140073029A1

公开(公告)日：2014-03-13

申请号：US14078772

申请日：2013-11-13

申请人： E I DU PONT DE NEMOURS AND COMPANY

发明人： ROBERT DICOSIMO ,  Scott D. Cunningham ,  Stephen R. Fahnestock ,  Tanja Maria Gruber ,  Mark S. Payne ,  Pierre E. Rouviere ,  Linda Jane Solomon ,  Hong Wang

IPC分类号： C12N9/18 ,  C12N9/42 ,  C07K16/16

CPC分类号： C12N9/18 ,  C07K16/16 ,  C07K19/00 ,  C07K2319/01 ,  C11D3/38636 ,  C11D3/38681 ,  C12N9/14 ,  C12N9/2437 ,  C12P7/40 ,  C12P7/54

摘要： Disclosed herein are compositions and methods to target enzymatic peracid production to a target surface. The peracid benefit agent produced by the targeted perhydrolytic enzyme can be use for a variety of applications such as bleaching, whitening, disinfecting, destaining, deodorizing, and combinations thereof. Specifically, a fusion protein comprising a perhydrolytic enzyme and at least one peptidic component having affinity for a target surface (excluding body surfaces and oral care surfaces) is used in combination with a suitable substrate and a source of peroxygen to enzymatically produce a peracid on or near the surface of the target material. In a preferred aspect, the target surface is a cellulosic material.

摘要翻译： 本文公开了将酶过氧酸生产靶向靶表面的组合物和方法。 由靶向的水解酶产生的过酸有益剂可用于各种应用，例如漂白，增白，消毒，脱色，除臭及其组合。 特别地，包含过水解酶和至少一种对靶表面（不包括身体表面和口腔护理表面）具有亲和性的肽成分的融合蛋白与合适的底物和过氧源组合使用以在其上或 靠近目标材料的表面。 在优选的方面，目标表面是纤维素材料。