公开(公告)号：US20160272676A1

公开(公告)日：2016-09-22

申请号：US15033940

申请日：2014-11-06

Applicant: EMD MILLIPORE CORPORATION

Inventor： Mikhail Kozlov ,  William Cataldo ,  Jeffrey Caron

IPC: C07K1/34 ,  C07K16/00 ,  C08F220/56 ,  C08F220/54 ,  C08F220/18 ,  B01J20/289 ,  C08J5/22 ,  C08F259/08 ,  B01D15/10 ,  B01D37/00 ,  B01J20/26 ,  B01J20/288 ,  C07K1/18 ,  C08F220/58

CPC classification number: C07K1/34 ,  B01D15/10 ,  B01D37/00 ,  B01J20/267 ,  B01J20/288 ,  B01J20/289 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/327 ,  B01J20/3276 ,  B01J20/3291 ,  B01J39/26 ,  B01J2220/80 ,  C07K1/18 ,  C07K16/00 ,  C08F220/18 ,  C08F220/54 ,  C08F220/56 ,  C08F220/58 ,  C08F259/08 ,  C08F2220/1825 ,  C08F2220/1858 ,  C08J5/2243 ,  C08J2351/00

Abstract: Novel compositions for re-moving impurities such as, protein aggregates, from a sample containing a protein of interest, e.g., an antibody. Such compositions can be used prior to the virus filtration step during protein purification, to remove aggregates and protect the virus filter from fouling, therefore improving virus filter capacity. A porous solid support including a co-polymer having at least two monomers, wherein at least one of the monomers comprises acrylamide and at least a second monomer comprises a hydrophobic binding group, where the solid support selectively binds protein aggregates, thereby to separate the monomeric protein of interest from the protein aggregates. The method can be performed under neutral to high pH and high conductivity conditions.

Abstract translation: 用于从含有目的蛋白质的样品例如抗体重新杂交诸如蛋白质聚集体的新型组合物。 这样的组合物可以在蛋白质纯化期间的病毒过滤步骤之前使用，以除去聚集体并保护病毒过滤器免于结垢，从而提高病毒过滤能力。 一种多孔固体支持物，包括具有至少两种单体的共聚物，其中所述单体中的至少一种包含丙烯酰胺，并且至少第二单体包含疏水性结合基团，其中所述固体支持物选择性地结合蛋白质聚集体，由此分离单体 来自蛋白质聚集体的目标蛋白质。 该方法可在中性至高pH和高电导条件下进行。

公开(公告)号：US09951101B2

公开(公告)日：2018-04-24

申请号：US15033940

申请日：2014-11-06

Applicant: EMD Millipore Corporation

Inventor： Mikhail Kozlov ,  William Cataldo ,  Jeffrey Caron

IPC: C07K1/34 ,  C08F220/56 ,  C08F220/58 ,  B01D15/10 ,  B01D37/00 ,  B01J20/26 ,  B01J20/288 ,  B01J20/289 ,  C07K1/18 ,  C07K16/00 ,  C08F220/18 ,  C08F220/54 ,  C08F259/08 ,  C08J5/22 ,  B01J39/26 ,  B01J20/32

CPC classification number: C07K1/34 ,  B01D15/10 ,  B01D37/00 ,  B01J20/267 ,  B01J20/288 ,  B01J20/289 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/327 ,  B01J20/3276 ,  B01J20/3291 ,  B01J39/26 ,  B01J2220/80 ,  C07K1/18 ,  C07K16/00 ,  C08F220/18 ,  C08F220/54 ,  C08F220/56 ,  C08F220/58 ,  C08F259/08 ,  C08F2220/1825 ,  C08F2220/1858 ,  C08J5/2243 ,  C08J2351/00

Abstract: Novel compositions for removing impurities such as, protein aggregates, from a sample containing a protein of interest, e.g., an antibody. Such compositions can be used prior to the virus filtration step during protein purification, to remove aggregates and protect the virus filter from fouling, therefore improving virus filter capacity. A porous solid support including a co-polymer having at least two monomers, wherein at least one of the monomers comprises acrylamide and at least a second monomer comprises a hydrophobic binding group, where the solid support selectively binds protein aggregates, thereby to separate the monomeric protein of interest from the protein aggregates. The method can be performed under neutral to high pH and high conductivity conditions.

公开(公告)号：US10570171B2

公开(公告)日：2020-02-25

申请号：US15920995

申请日：2018-03-14

Applicant: EMD Millipore Corporation

Inventor： Mikhail Kozlov ,  William Cataldo ,  Jeffrey Caron

IPC: C07K1/34 ,  C08F220/56 ,  C08F220/58 ,  B01J39/26 ,  B01J20/32 ,  B01D15/10 ,  B01D37/00 ,  B01J20/26 ,  B01J20/28 ,  C07K1/18 ,  C07K16/00 ,  C08F220/18 ,  C08F220/64 ,  C08F259/08 ,  C08J5/22 ,  B01J20/288 ,  B01J20/289 ,  C08F220/54

Abstract: Novel compositions for removing impurities such as, protein aggregates, from a sample containing a protein of interest, e.g., an antibody. Such compositions can be used prior to the virus filtration step during protein purification, to remove aggregates and protect the virus filter from fouling, therefore improving virus filter capacity. A porous solid support including a co-polymer having at least two monomers, wherein at least one of the monomers comprises acrylamide and at least a second monomer comprises a hydrophobic binding group, where the solid support selectively binds protein aggregates, thereby to separate the monomeric protein of interest from the protein aggregates. The method can be performed under neutral to high pH and high conductivity conditions.

公开(公告)号：US20180208624A1

公开(公告)日：2018-07-26

申请号：US15920995

申请日：2018-03-14

Applicant: EMD Millipore Corporation

Inventor： Mikhail Kozlov ,  William Cataldo ,  Jeffrey Caron

IPC: C07K1/34 ,  C08F259/08 ,  C08J5/22 ,  B01D15/10 ,  B01D37/00 ,  B01J20/288 ,  B01J20/289 ,  C07K1/18 ,  C07K16/00 ,  C08F220/18 ,  C08F220/54 ,  B01J20/26 ,  C08F220/58 ,  C08F220/56 ,  B01J20/32 ,  B01J39/26

CPC classification number: C07K1/34 ,  B01D15/10 ,  B01D37/00 ,  B01J20/267 ,  B01J20/288 ,  B01J20/289 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/327 ,  B01J20/3276 ,  B01J20/3291 ,  B01J39/26 ,  B01J2220/80 ,  C07K1/18 ,  C07K16/00 ,  C08F220/18 ,  C08F220/54 ,  C08F220/56 ,  C08F220/58 ,  C08F259/08 ,  C08F2220/1825 ,  C08F2220/1858 ,  C08J5/2243 ,  C08J2351/00

Abstract: Novel compositions for removing impurities such as, protein aggregates, from a sample containing a protein of interest, e.g., an antibody. Such compositions can be used prior to the virus filtration step during protein purification, to remove aggregates and protect the virus filter from fouling, therefore improving virus filter capacity. A porous solid support including a co-polymer having at least two monomers, wherein at least one of the monomers comprises acrylamide and at least a second monomer comprises a hydrophobic binding group, where the solid support selectively binds protein aggregates, thereby to separate the monomeric protein of interest from the protein aggregates. The method can be performed under neutral to high pH and high conductivity conditions.