Liquid single reagent for assays involving confining gels
    3.
    发明授权
    Liquid single reagent for assays involving confining gels 失效
    液体单一试剂用于测定凝胶

    公开(公告)号:US5068198A

    公开(公告)日:1991-11-26

    申请号:US298192

    申请日:1989-01-13

    IPC分类号: G01N33/543 G01N33/549

    摘要: Assay methods and compositions are provided for determining an analyte in a sample suspected of containing the analyte. The composition comprises in a novel single liquid reagent at least one specific binding pair (sbp) member and its complementary member wherein at least one sbp member is reversibly confined in a material that temporarily renders the confined sbp member incapable of binding with its complementary sbp member. At least one of the sbp members is bound to a member of a signal producing system capable of producing a detectable signal in relation to the amount of analyte in the sample. The confinement is reversed, any remaining members of the signal producing system are added, and the signal produced in relation to the amount of analyte is measured. Examples of the confining material are lipid bilayers, cells and gels.

    摘要翻译: 提供测定方法和组合物用于测定怀疑含有分析物的样品中的分析物。 组合物包含新颖的单液体试剂至少一种特异性结合对(sbp)构件及其互补构件,其中至少一个sbp构件可逆地限制在暂时使限制的sbp构件不能与其互补的sbp构件结合的材料中 。 sbp成员中的至少一个与能够产生与样品中分析物的量相关的可检测信号的信号产生系统的成员结合。 约束相反,添加信号产生系统的任何剩余成员,并且测量相对于分析物量产生的信号。 限制材料的实例是脂双层,细胞和凝胶。

    Charge effects in enzyme immunoassays
    4.
    发明授权
    Charge effects in enzyme immunoassays 失效
    酶免疫测定中的电荷效应

    公开(公告)号:US4287300A

    公开(公告)日:1981-09-01

    申请号:US61099

    申请日:1979-07-26

    摘要: A method for determining a member of a specific binding pair-ligand and receptor (antiligand). Reagents employed include a first modified member which provides an electrical field due to the presence of a plurality of ionic charges and a second modified member labeled with a component of a signal producing system, which system may have one or more components. The average proximity in the assay medium of the first and second modified members is related to the amount of analyte, where the observed signal from the signal producing system is related to the effect of the electrical field on the signal producing system.Also, compositions are provided, as well as reagents, in predetermined ratios for optimizing the signal response to variations in analyte concentration.

    摘要翻译: 用于测定特异性结合对 - 配体和受体(反配体)的成员的方法。 使用的试剂包括由于存在多个离子电荷而提供电场的第一改性构件和用信号产生系统的组分标记的第二修饰构件,该系统可以具有一个或多个组分。 第一和第二修饰构件的测定介质中的平均接近度与分析物的量相关,其中来自信号产生系统的观测信号与电场对信号产生系统的影响有关。 此外,提供了以预定比例的组合物以及试剂,以优化对分析物浓度变化的信号响应。

    Charge effects in enzyme immunoassays
    5.
    发明授权
    Charge effects in enzyme immunoassays 失效
    酶免疫测定中的电荷效应

    公开(公告)号:US4501692A

    公开(公告)日:1985-02-26

    申请号:US259629

    申请日:1982-05-01

    CPC分类号: G01N33/542

    摘要: A method for determining a member of a specific binding pair-ligand and receptor (antiligand). Reagents employed include a first modified member which provides an electrical field due to the presence of a plurality of ionic charges and a second modified member labeled with a component of a signal producing system, which system may have one or more components. The average proximity in the assay medium of the first and second modified members is related to the amount of analyte, where the observed signal from the signal producing system is related to the effect of the electrical field on the signal producing system.Also, compositions are provided, as well as reagents, in predetermined ratios for optimizing the signal response to variations in analyte concentration.

    摘要翻译: 用于测定特异性结合对 - 配体和受体(反配体)的成员的方法。 使用的试剂包括由于存在多个离子电荷而提供电场的第一改性构件和用信号产生系统的组分标记的第二修饰构件,该系统可以具有一个或多个组分。 第一和第二修饰构件的测定介质中的平均接近度与分析物的量相关,其中来自信号产生系统的观测信号与电场对信号产生系统的影响有关。 此外,提供了以预定比例的组合物以及试剂,以优化对分析物浓度变化的信号响应。

    Enzymatic poly-reactant channeling binding assay
    6.
    发明授权
    Enzymatic poly-reactant channeling binding assay 失效
    酶多反应物通道结合测定

    公开(公告)号:US4687735A

    公开(公告)日:1987-08-18

    申请号:US474906

    申请日:1983-03-14

    摘要: Improved sensitive immunoassays are provided involving channeling involving one, usually two enzymes, where the enzymes are related by the product of one enzyme being the substrate of the other enzyme. A dispersed aggregation is formed in the assay medium of (1) the analyte, (2) one of the enzymes bound to a second binding member ("SBM") (enzyme - SBM conjugate) which conjugate is non-covalently bound to a first binding member ("FBM"), and (3) a multiplied amount of the other enzyme bound in the complex. The large amount of enzyme or reactant in the complex is achieved by having a multiplicity of linkages binding the enzyme or reactant directly or indirectly to FBMs. The enzyme channeling provides for a detectable signal which can be related to the amount of analyte in the medium.

    摘要翻译: 提供了改进的敏感性免疫测定法,其涉及涉及一种通常两种酶的引导,其中酶与另一种酶的底物的酶的产物相关。 在(1)分析物的测定培养基中形成分散的聚集体,(2)与第二结合成员(“SBM”)结合的酶之一(酶-SMM缀合物),其结合物与第一结合物非共价结合 结合成员(“FBM”),和(3)复合物中结合的另一种酶的量。 复合物中大量的酶或反应物通过具有将酶或反应物直接或间接地结合到FBM的多重连接来实现。 酶通道提供可以与介质中分析物的量相关的可检测信号。

    Double antibody for enhanced sensitivity in immunoassay
    7.
    发明授权
    Double antibody for enhanced sensitivity in immunoassay 失效
    双抗体,增强免疫测定的灵敏度

    公开(公告)号:US4281061A

    公开(公告)日:1981-07-28

    申请号:US61542

    申请日:1979-07-27

    摘要: Method and compositions are provided for performing homogeneous immunoassays. The method involves having a signal producing system, which provides a detectable signal, which system includes a macromolecular member. The determination of the analyte, which is a member of a specific binding pair consisting of a ligand and its homologous receptor, is performed by creating an extensive matrix in the assay medium by having in the assay medium in addition to the analyte, ligand labeled with one of the members of the signal producing system, antiligand either present as the analyte or added, a polyvalent receptor for antiligand, the macromolecular member of the signal producing system, and any additional members of the signal producing system. The labeled ligand, antiligand, and polyvalent receptor for the antiligand create a matrix which modulates, e.g. inhibits, the approach of the macromolecular member of the signal producing system to the labeled ligand. The extent and degree of formation of the matrix is dependent upon the concentration of the analyte in the medium. By comparing the signal from an assay medium having an unknown amount of analyte, with a signal obtained from an assay medium having a known amount of analyte, the amount of analyte in the unknown sample may be determined qualitatively or quantitatively.Kits are provided having predetermined amounts of the various reagents to allow for enhanced sensitivity of the method.

    摘要翻译: 提供方法和组合物用于进行均匀的免疫测定。 该方法包括具有提供可检测信号的信号产生系统,该系统包括大分子成员。 由配体及其同源受体组成的特异性结合对成员的分析物的测定通过在测定培养基中通过在测定培养基中除了分析物之外产生广泛的基质来进行,所述分析物被标记有 信号生成系统的成员之一,反配位体作为分析物存在或添加,反配体的多价受体,信号产生系统的大分子成员以及信号产生系统的任何附加成员。 用于反配体的标记的配体,反配体和多价受体产生调节的基质。 抑制信号产生系统的大分子成员对标记的配体的接近。 基质的形成程度取决于培养基中分析物的浓度。 通过比较来自具有未知量分析物的测定培养基的信号与从具有已知量分析物的测定培养基获得的信号,可以定性或定量地确定未知样品中分析物的量。 提供具有预定量的各种试剂以提供该方法的增强灵敏度的试剂盒。

    Method and compositions for protecting anerobic microorganisms
    8.
    发明授权
    Method and compositions for protecting anerobic microorganisms 失效
    保护厌氧微生物的方法和组合物

    公开(公告)号:US4775626A

    公开(公告)日:1988-10-04

    申请号:US866824

    申请日:1986-05-23

    摘要: A method is disclosed for reducing the oxygen content of a medium in which are present cells, usually anaerobic microorganisms, to extend the time during which the cells remain viable. The method comprises having in fluid contact with the aqueous medium an effective amount of an oxidase and substrate for the oxidase. The oxidase and substrate for the oxidase in an aqueous medium can be in fluid, e.g., air, contact with a separately contained aqueous medium in which the cells are present. Alternatively, the cells can be present in the same aqueous medium as the oxidase and substrate for the oxidase. The aqueous medium containing the oxidase and the substrate for the oxidase can further contain a hydrogen peroxide scavenger in an effective amount. A composition in accordance with the invention comprises in a liquid medium viable cells, such as anaerobic microorganisms, an oxidase and its substrate in an amount sufficient to retain the viability of the cells, and a hydrogen peroxide scavenger in an amount sufficient to reduce the level of hydrogen peroxide generated by the oxidase. The method of the invention has particular application in transport media used for transporting microbiological specimens.

    摘要翻译: 公开了一种减少其中存在细胞(通常是厌氧微生物)延长细胞保持存活时间的培养基的氧含量的方法。 该方法包括与水性介质流体接触有效量的用于氧化酶的氧化酶和底物。 水性介质中氧化酶的氧化酶和底物可以是流体,例如空气,与存在细胞的单独含有的水性介质接触。 或者,细胞可以存在于与氧化酶和氧化酶底物相同的水性介质中。 含有氧化酶的水性介质和氧化酶底物还可以含有有效量的过氧化氢清除剂。 根据本发明的组合物在液体介质中包含足以保持细胞存活力的量的活细胞,例如厌氧微生物,氧化酶及其底物,以及足以降低水平的量的过氧化氢清除剂 的氧化酶产生的过氧化氢。 本发明的方法在用于运输微生物标本的运输介质中具有特殊的应用。

    Homogeneous enzyme specific binding assay on non-porous surface
    9.
    发明授权
    Homogeneous enzyme specific binding assay on non-porous surface 失效
    在无孔表面上的均相酶特异性结合测定

    公开(公告)号:US4629690A

    公开(公告)日:1986-12-16

    申请号:US561987

    申请日:1983-12-14

    摘要: Enzyme channeling assay is provided employing a solid non-porous surface to which is bound a member of a specific binding pair and an enzyme. A complementary member of a specific binding pair is conjugated to second enzyme, so that the amount of second enzyme which becomes bound to said solid non-porous surface by the binding of a specific binding pair(s) is related to the amount of analyte in the liquid assay medium with which the solid surface is in contact. The first and second enzymes are related by the product of one being the substrate of the other. The turnover of the substrate formed by one of the enzymes results in a detectable product, which can be related to the amount of analyte in the medium.

    摘要翻译: 使用结合特异性结合对和酶的成员的固体无孔表面提供酶引导测定。 特异性结合对的互补成员与第二酶结合,使得通过特异性结合对的结合而与所述固体无孔表面结合的第二酶的量与分析物的量相关 与固体表面接触的液体测定介质。 第一和第二酶与另一种酶的产物相关。 由酶之一形成的底物的营业额导致可检测的产物,其可与培养基中分析物的量相关。

    Monooxygenase assays
    10.
    发明授权
    Monooxygenase assays 失效
    单加氧酶测定

    公开(公告)号:US06632630B2

    公开(公告)日:2003-10-14

    申请号:US09820289

    申请日:2001-03-28

    IPC分类号: C12Q126

    摘要: Cytochrome P-450 assay methods and kits for the methods are provided employing a cytochrome P-450 enzyme, substrates characterized by having an oxidizable methylene group oxidized to an aldehyde and a fluorescent hydrazine. A fluorescent hydrazine is added to the reaction mixture and the resulting hydrazone analyzed by capillary electrophoresis. The method finds use in evaluating compounds for enzyme modulating activity.

    摘要翻译: 使用细胞色素P-450酶提供细胞色素P-450测定方法和用于该方法的试剂盒,其特征在于具有氧化成醛的可氧化亚甲基和荧光肼。 向反应混合物中加入荧光肼,通过毛细管电泳分析得到的腙。 该方法可用于评价化合物的酶调节活性