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10 条记录 (耗时 0.017 秒)

    Heterologous dimeric proteins produced in heterokaryons
    2.
    发明授权
    Heterologous dimeric proteins produced in heterokaryons 失效
    在异核子中产生的异源二聚体蛋白

    公开(公告)号：US5643745A

    公开(公告)日：1997-07-01

    申请号：US191337

    申请日：1994-02-03

    申请人： W. Dorsey Stuart

    发明人： W. Dorsey Stuart

    IPC分类号： C12N15/09 ,  C07K14/59 ,  C12N1/15 ,  C12N15/04 ,  C12N15/10 ,  C12N15/16 ,  C12P21/02 ,  C12P21/04 ,  C12R1/645 ,  C40B40/02

    CPC分类号： C40B40/02 ,  C07K14/59 ,  C12N15/04 ,  C12N15/1037

    摘要： The invention relates to a heterokaryotic filamentous fungus host capable of producing a heterologous heterodimer comprising at least two subunits. The heterokaryon contains a first and second nucleus; each nucleus contains an expression system for one subunit of the heterodimer. The heterokaryon is prepared by culturing together a first fungus host strain and a second fungus host strain that is homozygous with the first fungus host strain with respect to all heterokaryon compatibility alleles, wherein the first and second fungus host strains are cultured together under conditions wherein neither the first nor the second fungus strain can survive unless the heterokaryotic host is formed.

    摘要翻译： 本发明涉及能够产生包含至少两个亚基的异源二聚体的异源丝状真菌宿主。 异核包含第一和第二核; 每个核包含异二聚体的一个亚基的表达系统。 通过将第一真菌宿主菌株和与第一真菌宿主菌株纯合的第二真菌宿主菌株相对于所有异核相容性等位基因一起培养来制备异源核苷酸，其中第一和第二真菌宿主菌株在 除非形成异源宿主，否则第一种或第二种真菌菌株可以存活。

    Neurospora expression system
    3.
    发明授权
    Neurospora expression system 失效
    神经生物表达系统

    公开(公告)号：US5695965A

    公开(公告)日：1997-12-09

    申请号：US896455

    申请日：1992-06-02

    申请人： W. Dorsey Stuart ,  John M. Ivy ,  Kenneth Koo

    发明人： W. Dorsey Stuart ,  John M. Ivy ,  Kenneth Koo

    IPC分类号： C12N1/15 ,  C12N9/64 ,  C12N15/09 ,  C12N15/80 ,  C12R1/645 ,  C12P21/02

    CPC分类号： C12N9/6481 ,  C12N15/80

    摘要： Neurospora crassa transformations are employed for production of heterologous proteins, where DNA constructs are prepared using a foreign gene under transcriptional and translational regulatory regions functional in Neurospora. A functional signal sequence is provided for secretion of the heterologous gene product. An auxotrophic Neurospora host is employed and transformed by the combination of the subject construct and a complementing gene. The resulting transformants provide for efficient secretion of the heterologous product.

    摘要翻译： 粗糙脉孢菌转化用于生产异源蛋白质，其中使用外源基因在神经丝孢菌中具有功能的转录和翻译调节区制备DNA构建体。 提供功能信号序列用于分泌异源基因产物。 使用营养缺陷型神经孢子体宿主并通过受试者构建体和补体基因的组合进行转化。 得到的转化体提供异源产物的有效分泌。

    Monoclonal antibodies for DNA-RNA hybrid complexes and their uses
    6.
    发明授权
    Monoclonal antibodies for DNA-RNA hybrid complexes and their uses 失效
    DNA-RNA复合物的单克隆抗体及其用途

    公开(公告)号：US4865980A

    公开(公告)日：1989-09-12

    申请号：US155359

    申请日：1988-02-12

    申请人： W. Dorsey Stuart ,  Mark B. Frank

    发明人： W. Dorsey Stuart ,  Mark B. Frank

    IPC分类号： C12Q1/68 ,  G01N33/53

    CPC分类号： C12Q1/6804 ,  G01N33/5308 ,  Y10S530/864

    摘要： Monoclonal antibodies are provided capable of distinguishing DNA-RNA hybrid complexes from single stranded DNA and RNA and double stranded DNA and RNA. The antibodies find particular use in determining the presence of a specific nucleic acid sequence on a solid surface. Single stranded polynucleotide is fixed to a solid (gel) surface and then hybridized with the complementary probe. The hybrid complex specific monoclonal antibody is then added to bind to any hybrid complexes which have formed. By appropriate label, the hybrid complex may be visualized in a variety of ways.

    摘要翻译： 提供能够区分DNA-RNA复合物与单链DNA和RNA以及双链DNA和RNA的单克隆抗体。 抗体特别用于确定固体表面上特定核酸序列的存在。 将单链多核苷酸固定于固体（凝胶）表面，然后与互补探针杂交。 然后加入杂交复合物特异性单克隆抗体以结合已形成的任何杂合复合物。 通过适当的标签，混合复合物可以以各种方式可视化。

    Combinatorial metabolic libraries
    7.
    发明授权
    Combinatorial metabolic libraries 有权
    组合代谢库

    公开(公告)号：US06268140B1

    公开(公告)日：2001-07-31

    申请号：US09310398

    申请日：1999-05-12

    申请人： W. Dorsey Stuart

    发明人： W. Dorsey Stuart

    IPC分类号： C12Q168

    CPC分类号： C12N15/1058 ,  C07K14/59 ,  C12N15/04 ,  C12N15/1037 ,  C12N15/52 ,  C40B40/02

    摘要： The present invention provides methods and compositions for producing and screening combinatorial metabolic libraries of multimeric proteins. The present invention relies on the use of filamentous fingal heterokaryons that are produced using two or more parent strains into which a population of heterologous nucleic acids encoding components of a metabolic pathway have been introduced.

    摘要翻译： 本发明提供了用于产生和筛选多聚体蛋白质的组合代谢文库的方法和组合物。 本发明依赖于使用两个或多个亲本菌株产生的丝状的指甲异源核，其中已经引入编码代谢途径组分的异源核酸群。

    Monoclonal antibodies for DNA-RNA hybrid complexes and their uses
    10.
    发明授权
    Monoclonal antibodies for DNA-RNA hybrid complexes and their uses 失效
    DNA-RNA复合物的单克隆抗体及其用途

    公开(公告)号：US4732847A

    公开(公告)日：1988-03-22

    申请号：US454317

    申请日：1982-12-29

    申请人： W. Dorsey Stuart ,  Mark B. Frank

    发明人： W. Dorsey Stuart ,  Mark B. Frank

    IPC分类号： C12Q1/68 ,  G01N33/53 ,  C07K15/04 ,  G01N33/577

    CPC分类号： C12Q1/6804 ,  G01N33/5308 ,  Y10S435/81 ,  Y10S436/804 ,  Y10S436/808 ,  Y10S530/809 ,  Y10S530/864

    摘要： Monoclonal antibodies are provided capable of distinguishing DNA-RNA hybrid complexes from single stranded DNA and RNA and double stranded DNA and RNA. The antibodies find particular use in determining the presence of a specific nucleic acid sequence on a solid surface. Single stranded polynucleotide is fixed to a solid (gel) surface and then hybridized with the complementary probe. The hybrid complex specific monoclonal antibody is then added to bind to any hybrid complexes which have formed. By appropriate label, the hybrid complex may be visualized in a variety of ways.

    摘要翻译： 提供能够区分DNA-RNA复合物与单链DNA和RNA以及双链DNA和RNA的单克隆抗体。 抗体特别用于确定固体表面上特定核酸序列的存在。 将单链多核苷酸固定在固体（凝胶）表面上，然后与互补探针杂交。 然后加入杂交复合物特异性单克隆抗体以结合已形成的任何杂合复合物。 通过适当的标签，混合复合物可以以各种方式可视化。