公开(公告)号：US11701389B2

公开(公告)日：2023-07-18

申请号：US16083661

申请日：2017-03-09

申请人： Emory University ,  Children's Healthcare of Atlanta, Inc.

发明人： Jacques Galipeau ,  Raghavan Chinnadurai

IPC分类号： A61K35/28 ,  A01N1/02 ,  C12N5/0775

CPC分类号： A61K35/28 ,  A01N1/0215 ,  A01N1/0221 ,  A01N1/0226 ,  A01N1/0284 ,  C12N5/0663 ,  C12N2501/24 ,  C12N2523/00

摘要： This disclosure relates to methods of preserving mesenchymal stromal/stem cells (MSCs) for use in clinical applications. In certain embodiments, this disclosure relates to methods of preserving MSCs comprising mixing MSCs with interferon-gamma prior to cryopreserving, freezing, or cooling the MSCs to a temperature below zero degrees Celsius.

公开(公告)号：US20190099449A1

公开(公告)日：2019-04-04

申请号：US16083661

申请日：2017-03-09

申请人： Emory University ,  Children's Healthcare of Atlanta, Inc.

发明人： Jacques Galipeau ,  Raghavan Chinnadurai

IPC分类号： A61K35/28 ,  A01N1/02

摘要： This disclosure relates to methods of preserving mesenchymal stromal/stem cells (MSCs) for use in clinical applications. In certain embodiments, this disclosure relates to methods of preserving MSCs comprising mixing MSCs with interferon-gamma prior to cryopreserving, freezing, or cooling the MSCs to a temperature below zero degrees Celsius.

公开(公告)号：US09375465B2

公开(公告)日：2016-06-28

申请号：US14355673

申请日：2012-11-13

申请人： EMORY UNIVERSITY ,  CHILDREN'S HEALTHCARE OF ATLANTA, INC.

发明人： Jacques Galipeau ,  Hsiang-Chuan Hsieh

IPC分类号： A61K38/19 ,  A61K39/00 ,  C07K14/535 ,  C07K14/54 ,  A61K39/39 ,  A61K35/14 ,  A61K38/00

CPC分类号： A61K39/39 ,  A61K35/14 ,  A61K38/00 ,  A61K38/193 ,  A61K38/2046 ,  A61K39/0005 ,  A61K39/0011 ,  A61K39/245 ,  A61K2039/5158 ,  A61K2039/55522 ,  A61K2039/55527 ,  A61K2039/572 ,  C07K14/535 ,  C07K14/5418 ,  C07K2319/00

摘要： In certain embodiments, this disclosure relates to conjugates comprising GM-CSF and IL-7 and uses related thereto, e.g., enhancing the adaptive immune system. Typically the GM-CSF and IL-7 are connected by a polymer linker, e.g., polypeptide. In certain embodiments, the disclosure relates to nucleic acids encoding these polypeptide conjugates, vectors comprising nucleic acid encoding polypeptide conjugates, and protein expression systems comprising these vectors such as infectious viral particles and host cells comprising such a nucleic acids.

摘要翻译： 在某些实施方案中，本公开涉及包含GM-CSF和IL-7并且与其相关的缀合物，例如增强适应性免疫系统。 通常，GM-CSF和IL-7通过聚合物接头例如多肽连接。 在某些实施方案中，本公开涉及编码这些多肽缀合物的核酸，包含编码多肽缀合物的核酸的载体和包含这些载体的蛋白质表达系统，例如感染性病毒颗粒和包含这种核酸的宿主细胞。

公开(公告)号：US10072058B2

公开(公告)日：2018-09-11

申请号：US15142169

申请日：2016-04-29

申请人： Emory University ,  Children's Healthcare of Atlanta, Inc.

发明人： Baozhong Wang ,  Richard W. Compans ,  Jacques Galipeau ,  Jiusheng Deng

IPC分类号： C07K14/54 ,  C07K14/535 ,  C07K14/005 ,  C12N5/10 ,  C12N15/63 ,  C12N15/79 ,  C07K14/705 ,  C07K14/435 ,  A61K39/12 ,  C07K16/10 ,  A61K39/00

CPC分类号： C07K14/5406 ,  A61K39/12 ,  A61K2039/5258 ,  A61K2039/543 ,  A61K2039/55522 ,  A61K2039/55527 ,  A61K2039/575 ,  C07K14/005 ,  C07K14/435 ,  C07K14/535 ,  C07K14/70596 ,  C07K16/1063 ,  C07K2317/33 ,  C07K2317/76 ,  C07K2317/92 ,  C07K2319/02 ,  C07K2319/035 ,  C07K2319/912 ,  C12N15/63 ,  C12N15/79 ,  C12N2740/16023 ,  C12N2740/16034 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12N2740/16222

摘要： This disclosure relates to a GM-CSF and IL-4 conjugate fused to a glycolipid (GPI)-anchoring sequence that is incorporated into chimeric virus-like particles (VLPs) enriched with a viral protein, e.g., viral envelope protein or HIV envelope protein. In certain embodiments, the disclosure relates to methods of immunization with the chimeric VLPs disclosed herein. In certain embodiments, the disclosure relates to methods of immunization with disclosed HIV antigen containing VLPs through an intramuscular priming-intranasal boosting immunization route.

公开(公告)号：US20170304365A1

公开(公告)日：2017-10-26

申请号：US15607123

申请日：2017-05-26

申请人： EMORY UNIVERSITY ,  CHILDREN'S HEALTHCARE OF ATLANTA, INC.

发明人： Ian B. Copland ,  Jacques Galipeau

IPC分类号： A61K35/19 ,  A61L15/44 ,  C12N5/0775 ,  A61K31/727 ,  A61L17/00 ,  A61L15/40

CPC分类号： A61K35/19 ,  A61K31/727 ,  A61K38/1858 ,  A61L15/40 ,  A61L15/44 ,  A61L17/005 ,  A61L24/0005 ,  A61L24/0015 ,  A61L2300/406 ,  A61L2300/42 ,  C12N5/0018 ,  C12N5/0662 ,  C12N5/0663 ,  C12N5/0664 ,  C12N5/0665 ,  C12N5/0666 ,  C12N5/0667 ,  C12N5/0668 ,  C12N2502/115

摘要： In certain embodiments, this disclosure describes compositions comprising platelet lysates depleted of fibrinogen. In a further embodiment, the composition further comprises a cell culture medium component. This disclosure also provides a method for preparing the composition, comprising the steps of (a) lysing platelets providing a lysate; (b) removing cell debris; and (c) depleting fibrinogen by forming a removable mass by adding a metal salt such as calcium chloride. Furthermore, the disclosure also describes the product produced using said method.

公开(公告)号：US20170073387A1

公开(公告)日：2017-03-16

申请号：US15305714

申请日：2015-04-13

申请人： Emory University ,  Children's Healthcare of Atlanta, Inc.

发明人： Jacques Galipeau ,  Andrea Pennati

IPC分类号： C07K14/535 ,  A61K45/06 ,  C07K14/54 ,  A61K39/00

CPC分类号： C07K14/535 ,  A61K35/17 ,  A61K38/00 ,  A61K39/0008 ,  A61K45/06 ,  A61K2039/515 ,  C07K14/5406 ,  C07K14/5425 ,  C07K14/5443 ,  C07K14/55 ,  C07K2319/00 ,  A61K2300/00

摘要： This disclosure relates to recombinant proteins comprising a GM-CSF sequence and an interleukin sequence and nucleic acids related thereto. In certain embodiments, the disclosure relates to recombinant proteins comprises N-terminal sequences that are the result of improved production techniques and uses for treating or preventing autoimmune diseases such as multiple sclerosis and cancer.

摘要翻译： 本公开涉及包含GM-CSF序列和白介素序列的重组蛋白质以及与其相关的核酸。 在某些实施方案中，本公开涉及重组蛋白质，其包含作为治疗或预防自身免疫性疾病如多发性硬化和癌症的生产技术和用途的结果的N末端序列。

公开(公告)号：US20230277599A1

公开(公告)日：2023-09-07

申请号：US18296923

申请日：2023-04-06

申请人： Emory University ,  Children's Healthcare of Atlanta, Inc.

发明人： Jacques Galipeau ,  Raghavan Chinnadurai

IPC分类号： A61K35/28 ,  A01N1/02 ,  C12N5/0775

CPC分类号： A61K35/28 ,  A01N1/0215 ,  A01N1/0284 ,  A01N1/0226 ,  A01N1/0221 ,  C12N5/0663 ,  C12N2501/24 ,  C12N2523/00

摘要： This disclosure relates to methods of preserving mesenchymal stromal/stem cells (MSCs) for use in clinical applications. In certain embodiments, this disclosure relates to methods of preserving MSCs comprising mixing MSCs with interferon-gamma prior to cryopreserving, freezing, or cooling the MSCs to a temperature below zero degrees Celsius.

公开(公告)号：US20210277083A1

公开(公告)日：2021-09-09

申请号：US17324030

申请日：2021-05-18

申请人： Emory University ,  Children's Healthcare of Atlanta, Inc.

发明人： Jacques Galipeau ,  Jiusheng Deng

IPC分类号： C07K14/54 ,  C07K14/535 ,  A61K39/00 ,  A61K39/39 ,  C12N15/09 ,  C12N5/07 ,  C12N15/10 ,  A61K47/42 ,  A61K35/14 ,  A61K38/19 ,  A61K38/20

摘要： In certain embodiments, this disclosure relates to conjugates comprising GM-CSF and IL-4 and uses related thereto, e.g., enhancing the immune system. Typically, the GM-CSF and IL-4 are connected by a linker. In certain embodiments, the disclosure relates to isolated nucleic acids encoding these polypeptide conjugates, vectors comprising nucleic acid encoding polypeptide conjugates, and protein expression systems comprising these vectors such as infectious viral particles and host cells comprising such a nucleic acid.

公开(公告)号：US10464982B2

公开(公告)日：2019-11-05

申请号：US15305714

申请日：2015-04-13

申请人： Emory University ,  Children's Healthcare of Atlanta, Inc.

发明人： Jacques Galipeau ,  Andrea Pennati

IPC分类号： A61K38/19 ,  A61K38/20 ,  C07K14/535 ,  A61K35/17 ,  C07K14/54 ,  C07K14/55 ,  A61K39/00 ,  A61K45/06 ,  A61K38/00

摘要： This disclosure relates to recombinant proteins comprising a GM-CSF sequence and an interleukin sequence and nucleic acids related thereto. In certain embodiments, the disclosure relates to recombinant proteins comprises N-terminal sequences that are the result of improved production techniques and uses for treating or preventing autoimmune diseases such as multiple sclerosis and cancer.

公开(公告)号：US09657076B2

公开(公告)日：2017-05-23

申请号：US14436491

申请日：2013-10-23

申请人： EMORY UNIVERSITY ,  CHILDREN'S HEALTHCARE OF ATLANTA, INC

发明人： Jacques Galipeau ,  Jiusheng Deng

IPC分类号： A61K38/20 ,  C07K14/54 ,  C07K14/535 ,  A61K39/00 ,  A61K39/39 ,  A61K47/42 ,  C07K14/435 ,  C12N15/64 ,  A61K38/00

CPC分类号： C07K14/5406 ,  A61K35/14 ,  A61K38/00 ,  A61K38/193 ,  A61K38/2026 ,  A61K39/0011 ,  A61K39/39 ,  A61K47/42 ,  A61K2039/5152 ,  A61K2039/5156 ,  A61K2039/55522 ,  A61K2039/55527 ,  C07K14/535 ,  C07K2319/00

摘要： In certain embodiments, this disclosure relates to conjugates comprising GM-CSF and IL-4 and uses related thereto, e.g., enhancing the immune system. Typically the GM-CSF and IL-4 are connected by a linker. In certain embodiments, the disclosure relates to isolated nucleic acids encoding these polypeptide conjugates, vectors comprising nucleic acid encoding polypeptide conjugates, and protein expression systems comprising these vectors such as infectious viral particles and host cells comprising such a nucleic acids.