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1.
公开(公告)号:US20060228791A1
公开(公告)日:2006-10-12
申请号:US10516164
申请日:2003-06-25
申请人: Erwin Roggen , Nina Nilsson , Steffen Ernst , Carsten Andersen , Ninna Berg
发明人: Erwin Roggen , Nina Nilsson , Steffen Ernst , Carsten Andersen , Ninna Berg
摘要: The present invention relates to subtilase variants and subtilases with an altered immunogenicity, particularly subtilase variants and subtilases with a reduced allergenecity. Furthermore, the invention relates to expression of said subtilase variants and subtilases and to their use, such as in detergents and oral care products.
摘要翻译: 本发明涉及具有改变的免疫原性的枯草芽孢杆菌变体和减毒酶,特别是具有降低的变应原性的枯草芽孢杆菌变体和枯草芽孢杆菌素。 此外,本发明涉及所述脱羧酶变体和枯草芽孢杆菌的表达及其用途,例如洗涤剂和口腔护理产品。
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公开(公告)号:US20050181446A1
公开(公告)日:2005-08-18
申请号:US09957806
申请日:2001-09-21
申请人: Erwin Roggen , Steffen Ernst , Allan Svendsen , Esben Friis , Claus Osten
发明人: Erwin Roggen , Steffen Ernst , Allan Svendsen , Esben Friis , Claus Osten
IPC分类号: A21D2/26 , A21D8/04 , C07K1/04 , C07K5/103 , C07K5/11 , C07K5/113 , C07K14/00 , C07K16/40 , C07K16/42 , C11D3/386 , C12N9/02 , C12N9/20 , C12N15/53 , C12N15/55 , C12Q1/70 , C12N5/06 , C12P21/02 , G01N33/48 , G01N33/50 , G01N33/53 , G06F19/00
CPC分类号: C12N9/54 , A21D2/267 , A21D8/042 , A61K39/00 , C07K1/047 , C07K5/1008 , C07K5/101 , C07K5/1013 , C07K5/1019 , C07K5/1021 , C07K14/001 , C07K16/40 , C07K16/4283 , C11D3/38627 , C11D3/38645 , C12N9/0061 , C12N9/20 , C12Y110/03002 , C12Y304/21062 , Y10S435/911
摘要: The present invention relates to a method of selecting a protein variant having modified immunogenicity as compared to the parent protein comprising the steps obtaining antibody binding peptide sequences, using the sequences to localise epitope sequences on the 3-dimensional structure of parent protein, defining an epitope area including amino acids situated within 5 Å from the epitope amino acids constituting the epitope sequence, changing one or more of the amino acids defining the epitope area of the parent protein by genetical engineering mutations of a DNA sequence encoding the parent protein, introducing the mutated DNA sequence into a suitable host, culturing said host and expressing the protein variant, and evaluating the immunogenicity of the protein variant using the parent protein as reference. The invention further relates to the protein variant and use thereof, as well as to a method for producing said protein variant.
摘要翻译: 本发明涉及选择与亲本蛋白相比具有改良的免疫原性的蛋白质变体的方法,其包括获得抗体结合肽序列的步骤,使用该序列来定位母体蛋白的3维结构上的表位序列,限定表位 包括位于构成表位序列的表位氨基酸5埃范围内的氨基酸,通过编码母体蛋白质的DNA序列的遗传工程突变改变限定母体蛋白表位区域的一个或多个氨基酸,引入突变的 DNA序列转化为合适的宿主,培养所述宿主并表达蛋白质变体,并使用亲本蛋白作为参考来评估蛋白质变体的免疫原性。 本发明还涉及蛋白质变体及其用途,以及生产所述蛋白质变体的方法。
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公开(公告)号:US20070225207A1
公开(公告)日:2007-09-27
申请号:US11587239
申请日:2005-04-21
申请人: Vincent Batori , Erwin Roggen , Steffen Ernst , Stina Lyngstrand
发明人: Vincent Batori , Erwin Roggen , Steffen Ernst , Stina Lyngstrand
CPC分类号: C07K14/43531 , A61K39/00 , A61K2039/57
摘要: This invention concerns variants of a group 1 mite polypeptide, wherein the mature polypeptide of the variants comprise one or more mutations in the positions or corresponding to positions consisting of P11, I14, D15, L16, M19-P24, Q28, F37, S38, T43, A46-A49, Q53-L57, V63, A66-H69, H72, D74-R77, I80, Y82, Q84, H85, S92, I113, S114, P121, V124, K126, R128-A130, A132-S136, A139, L147, A149-H152, T157, Q160, N163, H170, A171, S178, V183, D184, R189, D193, F204, A206, N207, P217, L222 of SEQ ID NO: 1 or alternatively 11, 14, 15, 16, 19-24, 28, 37, 38, 43, 46-49, 53-57, 63, 66-69, 72, 74-77, 80, 82, 84, 85, 92, 113, 114, 121, 124, 126, 128-130, 132-136, 139, 147, 149-152, 157, 160, 163, 170, 178, 183, 189, 193, 204, 206, 207, 217, 222 of the mature Der p 1 polypeptide.
摘要翻译: 本发明涉及组1螨多肽的变体,其中变体的成熟多肽包含位置或对应于由P11,I14,D15,L16,M19-P24,Q28,F37,S38等组成的位置的一个或多个突变。 T43,A46-A49,Q53-L57,V63,A66-H69,H72,D74-R77,I80,Y82,Q84,H85,S92,I113,S114,P121,V124,K126,R128-A130,A132-S136, SEQ ID NO:1的A139,L147,A149-H152,T157,Q160,N163,H170,A171,S178,V183,D184,R189,D193,F204,A206,N207,P217,L222或者11,14,15 ,16,19-24,28,37,38,43,46-49,53-57,63,66-69,72,74-77,80,82,84,85,92,113,114,121 ,成熟Der的第124,126,128-130,132-136,139,147,149-152,157,160,163,170,178,183,189,193,204,206,207,217,222。 p 1多肽。
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4.
公开(公告)号:US20100279383A1
公开(公告)日:2010-11-04
申请号:US12831450
申请日:2010-07-07
申请人: Erwin Ludo Roggen , Nina Teeres Herne , Steffen Ernst , Carsten Andersen , Ninna Willestofte Berg
发明人: Erwin Ludo Roggen , Nina Teeres Herne , Steffen Ernst , Carsten Andersen , Ninna Willestofte Berg
IPC分类号: C12N9/54
摘要: The present invention relates to subtilase variants and subtilases with an altered immunogenicity, particularly subtilase variants and subtilases with a reduced allergenecity. Furthermore, the invention relates to expression of said subtilase variants and subtilases and to their use, such as in detergents and oral care products.
摘要翻译: 本发明涉及具有改变的免疫原性的枯草芽孢杆菌变体和减毒酶,特别是具有降低的变应原性的枯草芽孢杆菌变体和枯草芽孢杆菌素。 此外,本发明涉及所述脱羧酶变体和枯草芽孢杆菌的表达及其用途,例如洗涤剂和口腔护理产品。
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5.
公开(公告)号:US20120052538A1
公开(公告)日:2012-03-01
申请号:US13262002
申请日:2010-03-29
申请人: Per Munk Nielsen , Jesper Brask , Steffen Ernst
发明人: Per Munk Nielsen , Jesper Brask , Steffen Ernst
IPC分类号: C12P7/64
CPC分类号: C12P7/649 , C12P7/6454 , C12P7/6472 , Y02E50/13
摘要: The invention relates to a process for producing a triglyceride product comprising the steps of: (a) subjecting a triglyceride feedstock comprising at least 25 mole %, based on the total amount of acyl groups in the triglycerides, unsaturated C18 fatty acid residues to an alcoholy- sis reaction with an alcohol having 1 to 6 carbon atoms (C1-C6) to obtain a reaction mixture, 5 wherein the conversion is at least 65%; (b) separating from the reaction mixture produced in step (a) fractions comprising (i) unsaturated C18 fatty acid alkyl esters and (ii) glycerides; and (c) using the fractions (i) and (ii) or subfractions thereof in a condensation reaction catalyzed by a lipase to produce a triglyceride product comprising at least 80 mole % unsaturated fatty acids.
摘要翻译: 本发明涉及一种生产甘油三酸酯产品的方法,包括以下步骤:(a)将甘油三酯原料包含至少25摩尔%,基于甘油三酸酯中的酰基总量,不饱和C18脂肪酸残基至醇解 - 与具有1至6个碳原子的醇(C1-C6)反应得到反应混合物,其中转化率至少为65%; (b)从步骤(a)中产生的反应混合物中分离出包含(i)不饱和C18脂肪酸烷基酯和(ii)甘油酯的级分; 和(c)在由脂肪酶催化的缩合反应中使用级分(i)和(ii)或其亚级分,以产生包含至少80摩尔%不饱和脂肪酸的甘油三酯产物。
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公开(公告)号:US20100255153A1
公开(公告)日:2010-10-07
申请号:US12752207
申请日:2010-04-01
CPC分类号: A23L1/296 , A23L33/18 , A23L33/19 , A23L33/40 , C12N9/52 , C12N9/58 , C12N9/6424 , C12N9/6427
摘要: The present invention relates to an enzymatic process for making a milk-based protein hydrolysate and use of such hydrolysate, e.g., in an infant formula composition.
摘要翻译: 本发明涉及制备基于乳的蛋白质水解产物的酶法和使用这种水解产物,例如在婴儿配方组合物中。
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公开(公告)号:US06734310B2
公开(公告)日:2004-05-11
申请号:US10240980
申请日:2002-10-21
申请人: Knut Reiner , Steffen Ernst , Heinz Mustroph
发明人: Knut Reiner , Steffen Ernst , Heinz Mustroph
IPC分类号: C09B2306
CPC分类号: C09B23/02
摘要: The invention relates to cyanine dyes, which have alkyl substituents in the meso position of the methine chain, sulphoaryl groups as well as at least one reactive group which permits the binding to a target substance.
摘要翻译: 本发明涉及在次甲基链的中间位置具有烷基取代基,磺基芳基以及允许结合目标物质的至少一种反应性基团的花青染料。
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公开(公告)号:US09309549B2
公开(公告)日:2016-04-12
申请号:US11745537
申请日:2007-05-08
申请人: Lisbeth Kalum , Per Munk Nielsen , Steffen Ernst
发明人: Lisbeth Kalum , Per Munk Nielsen , Steffen Ernst
CPC分类号: C12P21/06 , A23L33/145 , C12N1/063 , C12N9/18
摘要: The present invention relates to a method for extracting components from yeast cells with a purified phospholipase.
摘要翻译: 本发明涉及一种用纯化磷脂酶从酵母细胞提取成分的方法。
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公开(公告)号:US20100285175A1
公开(公告)日:2010-11-11
申请号:US12744508
申请日:2008-12-02
申请人: Hanne Vang Hendriksen , Steffen Ernst , Reinhard Wilting , Jeppe Wegener Tams , Mette Oerhrstroem Runge , Helle Skov Guldager
发明人: Hanne Vang Hendriksen , Steffen Ernst , Reinhard Wilting , Jeppe Wegener Tams , Mette Oerhrstroem Runge , Helle Skov Guldager
CPC分类号: A23C9/1206 , A23C9/123
摘要: The present invention relates to a method for producing a dairy product using an enzyme having lactase activity.
摘要翻译: 本发明涉及使用具有乳糖酶活性的酶制造乳制品的方法。
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公开(公告)号:US20080279984A1
公开(公告)日:2008-11-13
申请号:US12015906
申请日:2008-01-17
申请人: Lisbeth Kalum , Steffen Ernst
发明人: Lisbeth Kalum , Steffen Ernst
IPC分类号: A23L1/217 , A23L1/2165
CPC分类号: C12Y302/01033 , A23L19/13 , A23L19/14 , A23L19/15 , A23L19/18 , A23L29/219 , C12Y302/01041 , C12Y302/01068 , C12Y302/01142
摘要: The present invention relates to methods for increasing the content of resistant starch in a potato product by enzymatic treatment. The invention further relates to potato products having an increased content of resistant starch.
摘要翻译: 本发明涉及通过酶处理增加马铃薯产品中抗性淀粉含量的方法。 本发明还涉及具有增加的抗性淀粉含量的马铃薯产品。
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