公开(公告)号：US20120031837A1

公开(公告)日：2012-02-09

申请号：US13274373

申请日：2011-10-17

申请人： David AMIR ,  Eugene Rosenberg ,  Gili Bittan-Banin

发明人： David AMIR ,  Eugene Rosenberg ,  Gili Bittan-Banin

IPC分类号： C02F3/00

CPC分类号： C02F3/006 ,  C02F2101/32 ,  C02F2103/365 ,  C02F2209/10 ,  C02F2209/40

摘要： A system and method for treating wastewater by continuously flowing wastewater into a chemostat and continuously discharging clean water out of the chemostat. The system can include sensors and an electronic controller for on-line measuring ambient parameters in the chemostat and adjusting the chemostat's operating conditions accordingly.

摘要翻译： 通过将废水连续流入恒温器并将洁净水连续地排出恒温器来处理废水的系统和方法。 该系统可以包括传感器和电子控制器，用于在线测量恒化器中的环境参数，并相应地调整恒化器的操作条件。

公开(公告)号：US5840547A

公开(公告)日：1998-11-24

申请号：US702711

申请日：1996-09-30

申请人： Eugene Rosenberg ,  Eliora Z. Ron

发明人： Eugene Rosenberg ,  Eliora Z. Ron

IPC分类号： A23L29/10 ,  A61K8/06 ,  A61K8/60 ,  A61K9/107 ,  A61Q5/02 ,  A61Q19/00 ,  B01F17/30 ,  C07K14/22 ,  C12P19/26 ,  A23L1/05

CPC分类号： C12P19/26 ,  A23L29/10 ,  A61K8/06 ,  A61K8/062 ,  A61K8/60 ,  A61Q19/00 ,  A61Q5/02 ,  Y10S514/938 ,  Y10S516/915 ,  Y10S530/825

摘要： A bioemulsifier designated Alasan or E-KA53 is produced from Acinetobacter radioresistens strain KA53. In its essentially pure form it has the characteristics of a molecular weight of from about 100,000 to 2,000,000 Daltons; emulsifying activity which increases with preheating at increasing temperatures--60.degree.-90.degree. C.; resistance to strong alkali while retaining increased emulsifying activity; reduced viscosity that varies as a function of temperature treatment; and emulsifying activity that varies as a function of pH and magnesium ions.

摘要翻译： PCT No.PCT / US96 / 00168 Sec。 371日期1996年9月30日 102（e）1996年9月30日PCT PCT 1996年1月5日PCT公布。 出版物WO96 / 20611 PCT 日期：1996年7月11日由不动杆菌不育杆菌菌株KA53生产名为Alasan或E-KA53的生物乳化剂。 在其基本上纯的形式中，其具有约100,000至2,000,000道尔顿的分子量的特征; 乳化活性随温度的升高而升高 - 60°-90°C。 耐强碱，同时保持乳化活性的增加; 作为温度处理的函数变化的粘度降低; 和作为pH和镁离子的函数而变化的乳化活性。

公开(公告)号：US5677161A

公开(公告)日：1997-10-14

申请号：US453815

申请日：1995-05-30

申请人： Eugene Rosenberg ,  Yuval Shoham

发明人： Eugene Rosenberg ,  Yuval Shoham

IPC分类号： C07K16/40 ,  C12N9/24 ,  D21C5/00 ,  C12N9/26

CPC分类号： C12Y302/01032 ,  C07K16/40 ,  C12N9/2402 ,  C12N9/248 ,  C12Y302/01008 ,  C12Y302/01055 ,  D21C5/005 ,  Y10S435/832

摘要： The invention relates to a preparation exhibiting enzymatic activity, which preparation has the capability of delignifying wood pulp at a temperature of at least 65.degree. C. and a pH of at least 9. Further, the invention relates to a method of producing said preparation by aerobically fermentating a selected Bacillus stearothermophilus strain. Furthermore, the invention relates to two isolated Bacillus stearothermophilus strains and mutants and variants thereof. The invention also relates to applications of the preparation of the invention, namely to a process comprising treatment of wood pulp with a preparation according to the invention, and a wood pulp and a fluff pulp treated with a preparation according to the invention, and also a paper, a board and a fluff made from a wood pulp treated with a preparation according to the invention.

摘要翻译： 本发明涉及一种具有酶活性的制剂，该制剂具有在至少65℃的温度和至少为9℃的温度下脱木质木浆的能力。此外，本发明涉及一种通过以下步骤制备所述制剂的方法： 有选择地发酵选择的嗜热脂肪芽孢杆菌菌株。 此外，本发明涉及两种分离的嗜热脂肪芽孢杆菌菌株及其突变体及其变体。 本发明还涉及本发明制剂的应用，即包括用本发明的制剂处理木浆的方法，以及用根据本发明的制剂处理的木浆和绒毛浆，以及 纸，板和由用根据本发明的制剂处理的木浆制成的绒毛。

公开(公告)号：US4311832A

公开(公告)日：1982-01-19

申请号：US146055

申请日：1980-05-14

申请人： David L. Gutnick ,  Eugene Rosenberg ,  Igal Belsky ,  Zosim Zinaida

发明人： David L. Gutnick ,  Eugene Rosenberg ,  Igal Belsky ,  Zosim Zinaida

IPC分类号： B01D21/01 ,  B01F17/00 ,  C02F1/52 ,  C02F1/68 ,  C08B37/00 ,  C09K8/90 ,  C12P1/04 ,  C12P19/26

CPC分类号： C09K8/905 ,  B01D21/01 ,  B01F17/005 ,  C02F1/5263 ,  C02F1/682 ,  C08B37/00 ,  C12P1/04 ,  C12P19/26 ,  Y10S435/822 ,  Y10S516/05 ,  Y10S516/06

摘要： Growth of Acinetobacter Sp. ATCC 31012 on various substrates and under varying conditions has been used to produce two classes of extracellular microbial protein-associated lipopolysaccharides (the "emulsans") which, on a weight-for-weight basis, are probably the most efficient emulsifiers discovered and which possess certain characteristics that permit these unique extracellular microbial lipopolysaccharides to be widely used in cleaning oil-contaminated vessels, oil spill management, and enhanced oil recovery by chemical flooding. These classes have been named .alpha.-emulsans and .beta.-emulsans, both of which have substantially the same polymer backbone but differ from each other in certain important structural aspects. Deproteinization of emulsans by hot phenol extraction produces the lipopolysaccharide components (the "apoemulsans") of each of such emulsans, which components have been shown to be completely N-acylated and partially O-acylated heteropolysaccharides made up of a major amounts of D-galactosamine and an aminouronic acid, the O-lipoacyl portions of such apoemulsans containing varying percentages of fatty acid esters in which the fatty acids contain from about 10 to about 18 carbon atoms. Base hydrolysis under mild conditions of the emulsans and apoemulsans produces derivatives (the ".psi.-emulsans" and "apo-.psi.-emulsans", respectively) which are completely N-acylated and partially to completely O-deacylated. Base hydrolysis under strong conditions of any of these products produces another derivate (the "proemulsans") which is completely O-deacylated and is partially N-deacylated. Emulsans and apoemulsans, both of which biopolymers are strongly anionic, exhibit a high degree of specificity in the emulsification of hydrocarbon substrates which contain both aliphatic and cyclic components. In addition, these extracellular microbial polysaccharides as well as their O-deacylated and N-deaclated derivatives are adsorbed on and capable of flocculating aluminosilicate ion-exchangers, such as kaolin and bentonite.

公开(公告)号：US4311831A

公开(公告)日：1982-01-19

申请号：US146054

申请日：1980-05-14

申请人： David L. Gutnick ,  Eugene Rosenberg ,  Igal Belsky ,  Zosim Zinaida

发明人： David L. Gutnick ,  Eugene Rosenberg ,  Igal Belsky ,  Zosim Zinaida

IPC分类号： B01D21/01 ,  B01F17/00 ,  C02F1/52 ,  C02F1/68 ,  C09K8/90 ,  C12P1/04 ,  C12P19/26 ,  C07H5/06

CPC分类号： C09K8/905 ,  B01D21/01 ,  B01F17/005 ,  C02F1/5263 ,  C02F1/682 ,  C12P1/04 ,  C12P19/26 ,  Y10S435/822 ,  Y10S516/06 ,  Y10S516/07 ,  Y10S530/825

摘要： Growth of Acinetobacter Sp. ATCC 31012 on various substrates and under varying conditions has been used to produce two classes of extracellular microbial protein-associated lipopolysaccharides (the "emulsans") which, on a weight-for-weight basis, are probably the most efficient emulsifiers discovered and which possess certain characteristics that permit these unique extracellular microbial lipopolysaccharides to be widely used in cleaning oil-contaminated vessels, oil spill management, and enhanced oil recovery by chemical flooding. These classes have been named .alpha.-emulsans and .beta.-emulsans, both of which have substantially the same polymer backbone but differ from each other in certain important structural aspects. Deproteinization of emulsans by hot phenol extraction produces the lipopolysaccharide components (the "apoemulsans") of each of such emulsans, which components have been shown to be completely N-acylated and partially O-acylated heteropolysaccharides made up of a major amounts of D-galactosamine and an aminouronic acid, the O-lipoacyl portions of such apoemulsans containing varying percentages of fatty acid esters in which the fatty acids contain from about 10 to about 18 carbon atoms. Base hydrolysis under mild conditions of the emulsans and apoemulsans produces derivatives (the ".psi.-emulsans" and "apo-.psi.-emulsans", respectively) which are completely N-acylated and partially to completely O-deacylated. Base hydrolysis under strong conditions of any of these products produces another derivate (the "proemulsans") which is completely O-deacylated and is partially N-deacylated. Emulsans and apoemulsans, both of which biopolymers are strongly anionic, exhibit a high degree of specificity in the emulsification of hydrocarbon substrates which contain both aliphatic and cyclic components. In addition, these extracellular microbial polysaccharides as well as their O-deacylated and N-deaclated derivatives are adsorbed on and capable of flocculating aluminosilicate ion-exchangers, such as kaolin and bentonite.

公开(公告)号：US20080308493A1

公开(公告)日：2008-12-18

申请号：US12155811

申请日：2008-06-10

申请人： David Amir ,  Eugene Rosenberg ,  Gili Bittan-Banin

发明人： David Amir ,  Eugene Rosenberg ,  Gili Bittan-Banin

IPC分类号： C02F3/02 ,  C02F9/14 ,  C02F9/02

CPC分类号： C02F3/006 ,  C02F2101/32 ,  C02F2103/365 ,  C02F2209/10 ,  C02F2209/40

摘要： A system and method for treating wastewater by continuously flowing wastewater into a chemostat and continuously discharging clean water out of the chemostat. The system can include sensors and an electronic controller for on-line measuring ambient parameters in the chemostat and adjusting the chemostat's operating conditions accordingly.

摘要翻译： 通过将废水连续流入恒温器并将洁净水连续地排出恒温器来处理废水的系统和方法。 该系统可以包括传感器和电子控制器，用于在线测量恒化器中的环境参数，并相应地调整恒化器的操作条件。

公开(公告)号：US20080277339A1

公开(公告)日：2008-11-13

申请号：US12081959

申请日：2008-04-24

申请人： Eugene Rosenberg

发明人： Eugene Rosenberg

IPC分类号： C02F3/28 ,  C02F1/40

CPC分类号： B08B9/093

摘要： A system and method for cleaning oil tanks comprising: a bio-reactor for producing bacterial cultures containing bio-emulsifiers from bacteria, air, water and sources of utilizable carbon, nitrogen and phosphate; a first pump for pumping the bacterial cultures from the bio-reactor through a first pipe into the oil tank; at least one spraying nozzle connected to the first pipe for spraying the bacterial cultures onto the oil tank's floor and walls under anaerobic conditions; and a second pump for pumping fluid out of the oil tank through a second pipe into a receiving container.

摘要翻译： 一种用于清洗油罐的系统和方法，包括：用于生产细菌培养物的生物反应器，其含有来自细菌，空气，水和可利用碳，氮和磷酸盐的来源的生物乳化剂; 用于将来自生物反应器的细菌培养物通过第一管道泵入油罐的第一泵; 连接到第一管的至少一个喷嘴，用于在厌氧条件下将细菌培养物喷洒到油箱的地板和墙壁上; 以及第二泵，用于通过第二管将液体从油箱中排出到容纳容器中。

公开(公告)号：US06887694B1

公开(公告)日：2005-05-03

申请号：US09710262

申请日：2000-11-10

申请人： Eugene Rosenberg ,  Eliora Ron ,  Elisha Orr ,  Yossi Paitan

发明人： Eugene Rosenberg ,  Eliora Ron ,  Elisha Orr ,  Yossi Paitan

IPC分类号： C12N15/52 ,  C12N9/00

CPC分类号： C12N15/52

摘要： There is provided DNA sequences isolated from Myxococcus xanthus partially encoding a functional portion of a polypeptide component required for the synthesis of antibiotic TA. Also provided are purified, isolated and cloned DNA sequences encoding a polypeptide component required for postmodification of antibiotic TA and encoding a gene product involved in the regulation of the biosynthesis of antibiotic TA. A purified, isolated and cloned DNA sequence having a DNA sequence (seq. ID No:2 and 20) encoding a polypeptide component required for encoding the TA gene cluster and any mutations thereof is provided. Also provided are methods of using the TA genes for combinatorial genetics and of using the TA genes encoding for synthesis and modification or regulation of antibiotic TA.

摘要翻译： 提供了从部分编码合成抗生素TA所需的多肽组分的功能部分的黄链球菌分离的DNA序列。 还提供了纯化，分离和克隆的DNA序列，其编码抗生素TA后修饰所需的多肽组分，并编码参与抗生素TA生物合成调控的基因产物。 提供了具有编码TA基因簇编码所需的多肽组分及其任何突变的DNA序列（序列号：2和20）的纯化，分离和克隆的DNA序列。 还提供了使用TA基因进行组合遗传学和使用编码抗生素TA的合成和修饰或调节的TA基因的方法。

公开(公告)号：US5780290A

公开(公告)日：1998-07-14

申请号：US461754

申请日：1995-06-05

申请人： Eugene Rosenberg ,  Eliora Z. Ron

发明人： Eugene Rosenberg ,  Eliora Z. Ron

IPC分类号： A62D3/00 ,  A62D3/02 ,  B09C1/10 ,  C02F1/68 ,  C02F3/34 ,  C12N1/20 ,  C12N1/26 ,  C12N11/08 ,  C12R1/01 ,  C12R1/05 ,  C12R1/38 ,  C12N1/00 ,  C12N1/38

CPC分类号： A62D3/02 ,  B09C1/10 ,  C02F1/682 ,  C02F3/34 ,  C02F3/344 ,  C12N1/26 ,  A62D2101/20 ,  Y10S210/922 ,  Y10S435/821 ,  Y10S435/826 ,  Y10S435/834

摘要： The present invention relates to compositions containing bacteria capable of degrading hydrocarbons, such as petroleum or petroleum products and methods for their use.

摘要翻译： 本发明涉及含有能够降解烃类的细菌的组合物，例如石油或石油产品及其使用方法。

公开(公告)号：US4311829A

公开(公告)日：1982-01-19

申请号：US146052

申请日：1980-05-14

申请人： David L. Gutnick ,  Eugene Rosenberg ,  Igal Belsky ,  Zosim Zinaida

发明人： David L. Gutnick ,  Eugene Rosenberg ,  Igal Belsky ,  Zosim Zinaida

IPC分类号： B01D21/01 ,  B01F17/00 ,  C02F1/52 ,  C02F1/68 ,  C08B37/00 ,  C09K8/90 ,  C12P1/04 ,  C12P19/26

CPC分类号： C09K8/905 ,  B01D21/01 ,  B01F17/005 ,  C02F1/5263 ,  C02F1/682 ,  C08B37/00 ,  C12P1/04 ,  C12P19/26 ,  Y10S435/822 ,  Y10S516/05 ,  Y10S516/06

摘要： Growth of Acinetobacter Sp. ATCC 31012 on various substrates and under varying conditions has been used to produce two classes of extracellular microbial protein-associated lipopolysaccharides (the "emulsans") which, on a weight-for-weight basis, are probably the most efficient emulsifiers discovered and which possess certain characteristics that permit these unique extracellular microbial lipopolysaccharides to be widely used in cleaning oil contaminated vessels, oil spill management, and enhanced oil recovery by chemical flooding. These classes have been named .alpha.-emulsans and .beta.-emulsans, both of which have substantially the same polymer backbone but differ from each other in certain important structural aspects. Deproteinization of emulsans by hot phenol extraction produces the lipopolysaccharide components (the "apoemulsans") of each of such emulsans, which components have been shown to be completely N-acylated and partially O-acylated heteropolysaccharides made up of a major amounts of D-galactosamine and an aminouronic acid, the O-lipoacyl portions of such apoemulsans containing varying percentages of fatty acid esters in which the fatty acids contain from about 10 to about 18 carbon atoms. Base hydrolysis under mild conditions of the emulsans and apoemulsans produces derivatives (the ".psi.-emulsans" and "apo-.psi.-emulsans", respectively) which are completely N-acylated and partially to completely O-deacylated. Base hydrolysis under strong conditions of any of these products produces another derivate (the "proemulsans") which is completely O-deacylated and is partially N-deacylated. Emulsans and apoemulsans, both of which biopolymers are strongly anionic, exhibit a high degree of specificity in the emulsification of hydrocarbon substrates which contain both aliphatic and cyclic components. In addition, these extracellular microbial polysaccharides as well as their O-deacylated and N-deaclated derivatives are adsorbed on and capable of flocculating aluminosilicate ion-exchangers, such as kaolin and bentonite.