中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

13 records (took 0.021 seconds)

    NUCLEIC ACID ENCODING REACTIONS
    1.
    发明申请
    NUCLEIC ACID ENCODING REACTIONS 有权

    公开(公告)号：US20220119874A1

    公开(公告)日：2022-04-21

    申请号：US17480067

    申请日：2021-09-20

    Applicant: Fluidigm Corporation

    Inventor： Megan Anderson ,  Peilin Chen ,  Brian Fowler ,  Robert C. Jones ,  Fiona Kaper ,  Ronald Lebofsky ,  Andrew May

    IPC: C12Q1/6855 ,  C12N15/65 ,  C12N15/66 ,  C12N15/10 ,  C12Q1/686 ,  C12Q1/6874

    Abstract: Described herein are methods useful for incorporating one or more adaptors and/or nucleotide tag(s) and/or barcode nucleotide sequence(s) one, or typically more, target nucleotide sequences. In particular embodiments, nucleic acid fragments having adaptors, e.g., suitable for use in high-throughput DNA sequencing are generated. In other embodiments, information about a reaction mixture is encoded into a reaction product. Also described herein are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, methods of the invention entail additionally carrying out bidirectional DNA sequencing. Also described herein are methods for encoding and detecting and/or quantifying alleles by primer extension.

    UNIVERSAL PROBE ASSAY METHODS
    5.
    发明申请
    UNIVERSAL PROBE ASSAY METHODS 审中-公开
    通用检测方法

    公开(公告)号：US20160348149A1

    公开(公告)日：2016-12-01

    申请号：US15144529

    申请日：2016-05-02

    Applicant: Fluidigm Corporation

    Inventor： Kenneth J. Livak ,  Jason A. A. West ,  Robert C. Jones

    IPC: C12Q1/68

    CPC classification number: C12Q1/682 ,  C12Q1/6876 ,  C12Q2600/178 ,  C12Q2525/155 ,  C12Q2525/307 ,  C12Q2537/161

    Abstract: Reagents and methods are provided for detecting the presence of a target polynucleotide in a sample are disclosed. In one aspect, a method for producing a labeled amplification product by amplifying a target nucleic acid sequence to produce an amplification product comprising the target sequence, a first probe-binding sequence 5′ to the target sequence, and a second probe-binding sequence 3′ to the target sequence, thereby producing an amplification product; and hybridizing a first detection probe to the amplification product, the first detection probe comprising a first segment that hybridizes to the first probe-binding sequence and a second segment that hybridizes to the second probe-binding sequence, thereby producing a labeled amplification product is disclosed.

    Abstract translation: 提供了用于检测样品中靶多核苷酸的存在的试剂和方法。 一方面，通过扩增靶核酸序列以产生包含靶序列的扩增产物，与靶序列的第一探针结合序列5'和第二探针结合序列3来产生标记的扩增产物的方法 '到靶序列，从而产生扩增产物; 并且将第一检测探针与扩增产物杂交，所述第一检测探针包含与第一探针结合序列杂交的第一区段和与第二探针结合序列杂交的第二区段，从而产生标记的扩增产物 。

Patent Agency Ranking