公开(公告)号：US20190078058A1

公开(公告)日：2019-03-14

申请号：US16180005

申请日：2018-11-05

Applicant: FUJIFILM CORPORATION ,  Kyoto University

Inventor： Hideaki KAGAWA ,  Shun GOTO ,  Souichi KOHASHI ,  Norio NAKATSUJI ,  Kazuhiro AIBA

IPC: C12N5/074

Abstract: The present disclosure provides a method for subculturing pluripotent stem cells suitable for mass culture. The method for subculturing pluripotent stem cells includes: a culture step of culturing pluripotent stem cells to obtain a cell aggregation; and a dividing step of dividing the cell aggregation by passing the cell aggregation through a mesh-like film, which has a plurality of through-holes each having an opening dimension of 30 μm to 80 μm, at a speed of 15 cm/sec to 150 cm/sec.

公开(公告)号：US20230092375A1

公开(公告)日：2023-03-23

申请号：US18058563

申请日：2022-11-23

Applicant: FUJIFILM Corporation

Inventor： Hirohisa HOKAZONO ,  Shun GOTO ,  Sohichiro NAKAMURA

IPC: D06N3/02 ,  D06N3/00 ,  D06N3/12

Abstract: An object of the present invention is to provide a composition capable of realizing an excellent matt tone of a leather surface in a case of being used as a synthetic leather or an artificial leather, a sheet-shaped molded body, an artificial leather, and a manufacturing method of a sheet-shaped molded body. The composition of the present invention is a composition used for a synthetic leather or an artificial leather, the composition containing a polypeptide, a fibrous substance other than the polypeptide, and, a plasticizer.

公开(公告)号：US20220267704A1

公开(公告)日：2022-08-25

申请号：US17742123

申请日：2022-05-11

Applicant: FUJIFILM Corporation

Inventor： Izumi OGURA ,  Keiji SHIGESADA ,  Shinji MIMA ,  Yuki IMAKURA ,  Shun GOTO ,  Nao YAMAZAKI

IPC: C12M1/04 ,  C12M1/00 ,  C12M1/12

Abstract: An object of the present invention is to provide a transportation instrument and a transportation method that enable transportation by using a cell culture container as it is. Further, an object of the present invention is to provide a transportation instrument and a transportation method that enable supply of oxygen required for respiration of cells. Further, an object of the present invention is to provide a transportation instrument and a transportation method that enable suppression of migration of a culture medium into and out of cells caused by the influence of an increase in internal pressure with respect to the temperature during transportation. According to the present invention, provided is a cell preservation or transportation instrument including a plurality of cell storage containers, a plate which has a plurality of recesses for holding the plurality of cell storage containers therein, and a flexible material sheet which seals upper opening portions of the plurality of cell storage containers and upper side wall portions formed by side walls of the recesses of the plate and allows ventilation between an inside and an outside of the plurality of cell storage containers.

公开(公告)号：US20190322977A1

公开(公告)日：2019-10-24

申请号：US16503473

申请日：2019-07-04

Applicant: FUJIFILM CORPORATION

Inventor： Hidetoshi TAKAYAMA ,  Shun GOTO

IPC: C12M1/34 ,  C12N5/074 ,  C12M1/12

Abstract: Provided is a cell culture apparatus including a cell supply unit that supplies cells; a culture medium supply unit that supplies a culture medium; an additive supply unit that supplies an additive for inducing the differentiation of undifferentiated cells; a stirring unit that stirs a processing target; a separation unit that separates a component contained in the processing target; a culture vessel that cultures the cells; a first flow channel that forms a circulation route passing through the cell supply unit, the stirring unit, the separation unit, and the culture vessel; a second flow channel that connects the culture medium supply unit and the first flow channel; a third flow channel that connects the additive supply unit and the first flow channel; and a control unit that controls the feeding of liquid through the first flow channel, the second flow channel, and the third flow channel.

公开(公告)号：US20190201585A1

公开(公告)日：2019-07-04

申请号：US16294931

申请日：2019-03-07

Applicant: FUJIFILM CORPORATION

Inventor： Koju ITO ,  Shun GOTO ,  Souichi KOHASHI ,  Kohji NAKAZAWA ,  Hiroshi YABU

IPC: A61L27/56 ,  C12N5/0775 ,  C12N5/077 ,  C12N5/071 ,  C12N5/078

CPC classification number: A61L27/56 ,  C08J9/28 ,  C12M1/00 ,  C12M3/00 ,  C12N5/0651 ,  C12N5/0656 ,  C12N5/0663 ,  C12N5/069

Abstract: Provided are a method for producing a cell tissue, including a culturing step of culturing cells capable of serving as a feeder inside opening pores and communicating pores of a porous film having a plurality of the opening pores provided on a surface thereof and the communicating pores communicating mutually adjacent opening pores with one another; and a porous film including a plurality of opening pores provided on a surface thereof and communicating pores communicating mutually adjacent opening pores with one another.

公开(公告)号：US20170306279A1

公开(公告)日：2017-10-26

申请号：US15649652

申请日：2017-07-14

Applicant: FUJIFILM CORPORATION

Inventor： Hideaki KAGAWA ,  Shun GOTO ,  Souichi KOHASHI ,  Hidekazu YAMAZAKI

IPC: C12M3/00 ,  C12M1/26 ,  C12M1/06 ,  C12M1/00

Abstract: A culture container has a first inflow port and a first outflow port. The first flow path connects the first outflow port to the first inflow port. A storage container is provided within the first flow path and has a second inflow port which is connected to the first outflow port and a second outflow port which is connected to the first inflow port. A second flow path connects a first region within the first flow path, and a second region within the first flow path. A division processing portion is provided within the second flow path, performs a division process of dividing a cell aggregation flowing in from the first flow path, and allows the cells subjected to the division process to flow out into the first flow path via the second region. A medium supply portion supplies a medium to the inside of the first flow path.

公开(公告)号：US20180216075A1

公开(公告)日：2018-08-02

申请号：US15939300

申请日：2018-03-29

Applicant: FUJIFILM Corporation

Inventor： Koju ITO ,  Shun GOTO ,  Souichi KOHASHI

IPC: C12N5/071 ,  C12M3/00 ,  C12M1/12

CPC classification number: C12N5/0671 ,  C12M21/08 ,  C12M23/34 ,  C12M25/02 ,  C12M25/14 ,  C12M33/00 ,  C12N1/04 ,  C12N5/0068 ,  C12N2533/40

Abstract: Provided is a composite binder material, a cell aggregate, a cell accumulation method, a cell preservation method, and a method of manufacturing a composite binder that can supply oxygen and nutrients to cells.A composite binder material comprises a binder material and a cell non-adhesive portion. A binder material formed in a membrane shape is provided between a first cell sheet and a second cell sheet. The cell non-adhesive portion formed at least in a portion of one membrane surface and/or other membrane surface of the binder material. A plurality of pores are formed in the binder material, and the pores open to the one membrane surface coming into contact with one sheet surface of the first cell sheet. The pores penetrate in a thickness direction and a surface direction along the one membrane surface. The porosity of the binder material is set to be at least 50%.