Method for making a biochip and biochip
    3.
    发明授权
    Method for making a biochip and biochip 失效
    制造生物芯片和生物芯片的方法

    公开(公告)号:US06803228B1

    公开(公告)日:2004-10-12

    申请号:US09868043

    申请日:2001-06-14

    IPC分类号: C12Q168

    摘要: The present invention relates to a method to produce a biochip and to a biochip, said biochip being composed particularly of biological probes grafted onto a conductive polymer. The method according to the invention comprises the following steps: a) structuring of a substrate so as to obtain on said substrate microtroughs comprising in their base a layer of a material capable of initiating and promoting the adhesion onto said layer of a film of a pyrrole and functionalised pyrrole copolymer by electropolymerisation, b) collective electropolymerisation, so as to form an electropolymerised film of a pyrrole and functionalised pyrrole copolymer on the base of said microtroughs, c) direct or indirect fixation of functionalised oligonucleotides by microdeposition or a liquid jet printing technique.

    摘要翻译: 本发明涉及一种生产生物芯片和生物芯片的方法,所述生物芯片特别由接枝在导电聚合物上的生物探针组成。本发明的方法包括以下步骤:a)将基底结构化成 在所述基材微通道上获得能够通过电聚合引发和促进在所述吡咯和官能化吡咯共聚物的膜上的粘合层的材料层,b)集体电聚合,以便形成电聚合膜 在所述微通道的基底上的吡咯和官能化的吡咯共聚物,c)通过微沉积或液体喷射印刷技术直接或间接固定官能化的寡核苷酸。

    Device comprising a plurality of analysis sites on a support
    4.
    发明授权
    Device comprising a plurality of analysis sites on a support 失效
    设备包括在支持体上的多个分析位置

    公开(公告)号:US06902705B1

    公开(公告)日:2005-06-07

    申请号:US09805772

    申请日:1999-09-15

    摘要: The subject of the invention is a device for chemical or biological analysis comprising a carrier (21) containing a plurality of analysis sites able to fix a chemical or biological reagent, in which the analysis sites are formed of microdishes (23) hollowed out of the carrier, the side walls and the bottom of the microdishes and the areas of the carrier surface surrounding each microdish, called microdish edges, being made in at least one hydrophilic material (24), and the planar areas of the carrier arranged between the areas surrounding the microdishes being made in a hydrophobic material (27).Drops (29) of reagent are therefore guided into the microdishes (23) on account of the hydrophobic areas (27). It is therefore possible to increase the density of analysis sites on the carrier.

    摘要翻译: 本发明的主题是用于化学或生物分析的装置,其包括载体(21),载体(21)含有能够固定化学或生物试剂的多个分析位点,其中所述分析位点由从所述化学或生物试剂中掏空的微粒(23)形成 载体,侧壁和微粒的底部以及围绕每个微粒的载体表面的区域(称为微米边缘)在至少一个亲水材料(24)中制成,并且载体的平面区域布置在周围的区域 微粒在疏水材料(27)中制成。 因此,由于疏水区域(27),试剂的滴液(29)因此被导入微量清洗液(23)。 因此,可以增加载体上的分析位点的密度。