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公开(公告)号：US07803578B2
公开(公告)日：2010-09-28
申请号：US11947955
申请日：2007-11-30
申请人： Fumiaki Watanabe , Dai Ujihara , Miki Sakai , Fujio Yu , Tetsuji Nakamura
发明人： Fumiaki Watanabe , Dai Ujihara , Miki Sakai , Fujio Yu , Tetsuji Nakamura
摘要： The present invention provides: a protein having an improved nitrile hydratase activity, whereby heat resistance has been improved when compared with a wild-type nitrile hydratase activity, wherein the amino acid sequence of a nitrile hydratase is modified; a gene DNA encoding the above protein; a recombinant vector having the above gene DNA; a transformant or transductant having the above recombinant vector; a nitrile hydratase collected from a culture of the above transformant or transductant, and a production method thereof; and a method for producing an amide compound.
摘要翻译： 本发明提供：腈水合酶活性提高的蛋白质，与野生型腈水合酶活性相比，耐水性得到改善，其中腈水合酶的氨基酸序列被修饰; 编码上述蛋白质的基因DNA; 具有上述基因DNA的重组载体; 具有上述重组载体的转化体或转导体; 从上述转化体或转导体的培养物收集的腈水合酶及其制备方法; 和酰胺化合物的制造方法。
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公开(公告)号：US20090162894A1
公开(公告)日：2009-06-25
申请号：US11947955
申请日：2007-11-30
申请人： Fumiaki Watanabe , Dai Ujihara , Miki Sakai , Fujio Yu , Tetsuji Nakamura
发明人： Fumiaki Watanabe , Dai Ujihara , Miki Sakai , Fujio Yu , Tetsuji Nakamura
摘要： The present invention provides: a protein having an improved nitrile hydratase activity, whereby heat resistance has been improved when compared with a wild-type nitrile hydratase activity, wherein the amino acid sequence of a nitrile hydratase is modified; a gene DNA encoding the above protein; a recombinant vector having the above gene DNA; a transformant or transductant having the above recombinant vector; a nitrile hydratase collected from a culture of the above transformant or transductant, and a production method thereof; and a method for producing an amide compound.
摘要翻译： 本发明提供：腈水合酶活性提高的蛋白质，与野生型腈水合酶活性相比，耐水性得到改善，其中腈水合酶的氨基酸序列被修饰; 编码上述蛋白质的基因DNA; 具有上述基因DNA的重组载体; 具有上述重组载体的转化体或转导体; 从上述转化体或转导体的培养物收集的腈水合酶及其制备方法; 和酰胺化合物的制造方法。
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公开(公告)号：US20070231868A1
公开(公告)日：2007-10-04
申请号：US11569529
申请日：2005-05-26
申请人： Fumiaki Watanabe , Dai Ujihara , Miki Sakai , Fujio Yu , Tetsuji Nakamura
发明人： Fumiaki Watanabe , Dai Ujihara , Miki Sakai , Fujio Yu , Tetsuji Nakamura
摘要： The present invention provides: a protein having an improved nitrile hydratase activity, whereby heat resistance has been improved when compared with a wild-type nitrile hydratase activity, wherein the amino acid sequence of a nitrile hydratase is modified; a gene DNA encoding the above protein; a recombinant vector having the above gene DNA; a transformant or transductant having the above recombinant vector; a nitrile hydratase collected from a culture of the above transformant or transductant, and a production method thereof; and a method for producing an amide compound.
摘要翻译： 本发明提供：腈水合酶活性提高的蛋白质，与野生型腈水合酶活性相比，耐水性得到改善，其中腈水合酶的氨基酸序列被修饰; 编码上述蛋白质的基因DNA; 具有上述基因DNA的重组载体; 具有上述重组载体的转化体或转导体; 从上述转化体或转导体的培养物收集的腈水合酶及其制备方法; 和酰胺化合物的制造方法。
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公开(公告)号：US07645605B2
公开(公告)日：2010-01-12
申请号：US11569529
申请日：2005-05-26
申请人： Fumiaki Watanabe , Dai Ujihara , Miki Sakai , Fujio Yu , Tetsuji Nakamura
发明人： Fumiaki Watanabe , Dai Ujihara , Miki Sakai , Fujio Yu , Tetsuji Nakamura
摘要： The present invention provides: a protein having an improved nitrile hydratase activity, whereby heat resistance has been improved when compared with a wild-type nitrile hydratase activity, wherein the amino acid sequence of a nitrile hydratase is modified; a gene DNA encoding the above protein; a recombinant vector having the above gene DNA; a transformant or transductant having the above recombinant vector; a nitrile hydratase collected from a culture of the above transformant or transductant, and a production method thereof; and a method for producing an amide compound.
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公开(公告)号：US06617139B1
公开(公告)日：2003-09-09
申请号：US09958969
申请日：2001-10-16
申请人： Tetsuji Nakamura , Fujio Yu
发明人： Tetsuji Nakamura , Fujio Yu
IPC分类号： C12P1304
摘要： The present invention relates to a novel protein having amidase activity that stereoselectively hydrolyzes &agr;-amino acid amides and &agr;-hydroxy acid amides, and a gene encoding said protein.
摘要翻译： 本发明涉及具有立体选择性水解α-氨基酸酰胺和α-羟基酰胺的酰胺酶活性的新型蛋白质，以及编码所述蛋白质的基因。
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6.发明授权Process for the production of 4-halo-3-hydroxybutyronitrile with Corynebacterium or Microbacterium 失效用棒状杆菌或微杆菌生产4-卤代-3-羟基丁腈的方法
公开(公告)号：US5166061A
公开(公告)日：1992-11-24
申请号：US555001
申请日：1990-07-20
申请人： Tetsuji Nakamura , Fujio Yu , Ichiro Watanabe
发明人： Tetsuji Nakamura , Fujio Yu , Ichiro Watanabe
CPC分类号： C12P13/002 , Y10S435/822 , Y10S435/843
摘要： A process for the production of a 4-halo-3-hydroxybutyronitrile which comprises reacting a 1,3-dihalo-2-propanol with a dehalogenating enzyme originating from a Corynebacterium or a Microbacterium in the presence of an alkali cyanide to thereby convert the 1,3-dihalo-2-propanol into the 4-halo-3-hydroyxbutyronitrile and collecting the product thus formed is disclosed. According to this process, a 4-halo-3-hydroxybutyronitrile which is highly useful in the syntheses of various medicines and physiologically active substances can be easily and efficiently produced from an inexpensive starting material.
摘要翻译： 一种4-卤代-3-羟基丁腈的制备方法，其包括在碱金属氰化物存在下使1,3-二卤代-2-丙醇与源自棒杆菌或微杆菌的脱卤素酶反应，从而将1 ，3-二卤代-2-丙醇加入到4-卤代-3-羟基丁腈中并收集如此形成的产品。 根据该方法，可以从廉价的原料容易且有效地制备在各种药物和生理活性物质的合成中非常有用的4-卤代-3-羟基丁腈。
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7.发明授权
公开(公告)号：US5210031A
公开(公告)日：1993-05-11
申请号：US830516
申请日：1992-02-03
申请人： Tetsuji Nakamura , Fujio Yu
发明人： Tetsuji Nakamura , Fujio Yu
IPC分类号： C12P13/00
CPC分类号： C12P13/002 , Y10S435/822 , Y10S435/843 , Y10S435/844 , Y10S435/845 , Y10S435/846
摘要： A process for the production of a 4-halo-3-hydroxybutyronitrile which comprises reacting an epihalohydrin with a halohydrin-halide-lyase originating from a microorganism selected from the group consisting of: Corynebacterium sp. N-2354 FERM BP-2726 and Microbacterium sp. N-4701 FERM BP-2644 in the presence of an alkali cyanide to thereby convert the epihalohydrin into the 4-halo-3-hydroxybutyronitrile and collecting the product thus formed is disclosed. According to this process, a 4-halo-3-hydroxybutyronitrile which is highly useful in the syntheses of various medicines and physiologically active substances can be easily and efficiently produced from an inexpensive starting material.
摘要翻译： 一种制备4-卤代-3-羟基丁腈的方法，其包括使表卤代醇与源自选自以下的微生物的卤代醇 - 卤化物裂解酶反应：棒状杆菌属 N-2354 FERM BP-2726和Microbacterium sp。 N-4701 FERM BP-2644在碱金属氰化物的存在下，从而将表卤代醇转化成4-卤代-3-羟基丁腈并收集如此形成的产品。 根据该方法，可以从廉价的原料容易且有效地制备在各种药物和生理活性物质的合成中非常有用的4-卤代-3-羟基丁腈。
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公开(公告)号：US07098889B2
公开(公告)日：2006-08-29
申请号：US10606701
申请日：2003-06-26
申请人： Takashi Inui , Tetsuji Nakamura
发明人： Takashi Inui , Tetsuji Nakamura
CPC分类号： G06F3/038
摘要： A device, method, and computer program product for a self-calibrating input device are provided. The input device includes a designated value change unit operable to change a designated value using a variation amount associated with the designated value, the designated value being changed using a predetermined first variation amount when a first designated value change directive is received from an operator, and a variation amount change unit operable to alter the variation amount associated with the designated value from the predetermined first variation amount to a smaller second variation amount when a second designated value change directive that is opposite the first designated value change directive is received from the operator within a predetermined variation amount change period.
摘要翻译： 提供了一种用于自校准输入设备的设备，方法和计算机程序产品。 所述输入装置包括指定值改变单元，其可操作以使用与所述指定值相关联的变化量来改变指定值，当从操作者接收到第一指定值改变指令时，所述指定值使用预定的第一变化量进行变化;以及 变更量变更单元，用于当从所述操作者接收到与所述第一指定值变更指令相反的第二指定值变更指令时，将与所述指定值相关联的所述变更量从所述预定第一变更量变更为较小的第二变更量 预定变化量变化期间。
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公开(公告)号：US5215897A
公开(公告)日：1993-06-01
申请号：US525302
申请日：1990-05-17
CPC分类号： C12N9/80 , C12P13/04 , C12P41/006 , Y10S435/828 , Y10S435/874
摘要： A process for producing an L-amino acid from the corresponding DL- and/or L-amino acid amide represented by the general formula: ##STR1## wherein R is a substituted or unsubstituted alkyl group having one to 4 carbon atoms, a substituted or unsubstituted phenyl group, or a substituted or unsubstituted aralkyl group, by action of an enzyme having hydrolytic activity to L-amino acid amides which is produced by Enterobacter cloacae or Pseudomonas sp.
摘要翻译： 由下列通式表示的相应的DL-和/或L-氨基酸酰胺生产L-氨基酸的方法：其中R是取代或未取代的具有1至4个碳原子的烷基，取代或 未取代的苯基或取代或未取代的芳烷基，通过对由阴沟肠杆菌或假单胞菌属（Pseudomonas sp。）产生的L-氨基酸酰胺具有水解活性的酶的作用。
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10.发明授权公开(公告)号：US08728792B2
公开(公告)日：2014-05-20
申请号：US12691443
申请日：2010-01-21
摘要： The present invention provides a modified ethylenediamine-N,N′-disuccinate:ethylenediamine lyase. The present invention also provides a protein that comprises the amino acid sequence represented by SEQ ID NO: 1; or a protein that comprises an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 1 by deletion, substitution, or addition of one or more amino acid residues, and has an ethylenediamine-N,N′-disuccinate:ethylenediamine lyase activity.
摘要翻译： 本发明提供了改性的乙二胺-N，N'-二琥珀酸盐：乙二胺裂解酶。 本发明还提供了包含SEQ ID NO：1所示的氨基酸序列的蛋白质; 或包含通过缺失，取代或添加一个或多个氨基酸残基而衍生自SEQ ID NO：1所示氨基酸序列的氨基酸序列的蛋白质，并且具有乙二胺-N，N'-二琥珀酸盐：乙二胺 裂解酶活性。
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