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1.发明授权Process for the production of 4-halo-3-hydroxybutyronitrile with Corynebacterium or Microbacterium 失效用棒状杆菌或微杆菌生产4-卤代-3-羟基丁腈的方法
公开(公告)号：US5166061A
公开(公告)日：1992-11-24
申请号：US555001
申请日：1990-07-20
申请人： Tetsuji Nakamura , Fujio Yu , Ichiro Watanabe
发明人： Tetsuji Nakamura , Fujio Yu , Ichiro Watanabe
CPC分类号： C12P13/002 , Y10S435/822 , Y10S435/843
摘要： A process for the production of a 4-halo-3-hydroxybutyronitrile which comprises reacting a 1,3-dihalo-2-propanol with a dehalogenating enzyme originating from a Corynebacterium or a Microbacterium in the presence of an alkali cyanide to thereby convert the 1,3-dihalo-2-propanol into the 4-halo-3-hydroyxbutyronitrile and collecting the product thus formed is disclosed. According to this process, a 4-halo-3-hydroxybutyronitrile which is highly useful in the syntheses of various medicines and physiologically active substances can be easily and efficiently produced from an inexpensive starting material.
摘要翻译： 一种4-卤代-3-羟基丁腈的制备方法，其包括在碱金属氰化物存在下使1,3-二卤代-2-丙醇与源自棒杆菌或微杆菌的脱卤素酶反应，从而将1 ，3-二卤代-2-丙醇加入到4-卤代-3-羟基丁腈中并收集如此形成的产品。 根据该方法，可以从廉价的原料容易且有效地制备在各种药物和生理活性物质的合成中非常有用的4-卤代-3-羟基丁腈。
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2.发明授权
公开(公告)号：US5210031A
公开(公告)日：1993-05-11
申请号：US830516
申请日：1992-02-03
申请人： Tetsuji Nakamura , Fujio Yu
发明人： Tetsuji Nakamura , Fujio Yu
IPC分类号： C12P13/00
CPC分类号： C12P13/002 , Y10S435/822 , Y10S435/843 , Y10S435/844 , Y10S435/845 , Y10S435/846
摘要： A process for the production of a 4-halo-3-hydroxybutyronitrile which comprises reacting an epihalohydrin with a halohydrin-halide-lyase originating from a microorganism selected from the group consisting of: Corynebacterium sp. N-2354 FERM BP-2726 and Microbacterium sp. N-4701 FERM BP-2644 in the presence of an alkali cyanide to thereby convert the epihalohydrin into the 4-halo-3-hydroxybutyronitrile and collecting the product thus formed is disclosed. According to this process, a 4-halo-3-hydroxybutyronitrile which is highly useful in the syntheses of various medicines and physiologically active substances can be easily and efficiently produced from an inexpensive starting material.
摘要翻译： 一种制备4-卤代-3-羟基丁腈的方法，其包括使表卤代醇与源自选自以下的微生物的卤代醇 - 卤化物裂解酶反应：棒状杆菌属 N-2354 FERM BP-2726和Microbacterium sp。 N-4701 FERM BP-2644在碱金属氰化物的存在下，从而将表卤代醇转化成4-卤代-3-羟基丁腈并收集如此形成的产品。 根据该方法，可以从廉价的原料容易且有效地制备在各种药物和生理活性物质的合成中非常有用的4-卤代-3-羟基丁腈。
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公开(公告)号：US07645605B2
公开(公告)日：2010-01-12
申请号：US11569529
申请日：2005-05-26
申请人： Fumiaki Watanabe , Dai Ujihara , Miki Sakai , Fujio Yu , Tetsuji Nakamura
发明人： Fumiaki Watanabe , Dai Ujihara , Miki Sakai , Fujio Yu , Tetsuji Nakamura
摘要： The present invention provides: a protein having an improved nitrile hydratase activity, whereby heat resistance has been improved when compared with a wild-type nitrile hydratase activity, wherein the amino acid sequence of a nitrile hydratase is modified; a gene DNA encoding the above protein; a recombinant vector having the above gene DNA; a transformant or transductant having the above recombinant vector; a nitrile hydratase collected from a culture of the above transformant or transductant, and a production method thereof; and a method for producing an amide compound.
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公开(公告)号：US07803578B2
公开(公告)日：2010-09-28
申请号：US11947955
申请日：2007-11-30
申请人： Fumiaki Watanabe , Dai Ujihara , Miki Sakai , Fujio Yu , Tetsuji Nakamura
发明人： Fumiaki Watanabe , Dai Ujihara , Miki Sakai , Fujio Yu , Tetsuji Nakamura
摘要： The present invention provides: a protein having an improved nitrile hydratase activity, whereby heat resistance has been improved when compared with a wild-type nitrile hydratase activity, wherein the amino acid sequence of a nitrile hydratase is modified; a gene DNA encoding the above protein; a recombinant vector having the above gene DNA; a transformant or transductant having the above recombinant vector; a nitrile hydratase collected from a culture of the above transformant or transductant, and a production method thereof; and a method for producing an amide compound.
摘要翻译： 本发明提供：腈水合酶活性提高的蛋白质，与野生型腈水合酶活性相比，耐水性得到改善，其中腈水合酶的氨基酸序列被修饰; 编码上述蛋白质的基因DNA; 具有上述基因DNA的重组载体; 具有上述重组载体的转化体或转导体; 从上述转化体或转导体的培养物收集的腈水合酶及其制备方法; 和酰胺化合物的制造方法。
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公开(公告)号：US20090162894A1
公开(公告)日：2009-06-25
申请号：US11947955
申请日：2007-11-30
申请人： Fumiaki Watanabe , Dai Ujihara , Miki Sakai , Fujio Yu , Tetsuji Nakamura
发明人： Fumiaki Watanabe , Dai Ujihara , Miki Sakai , Fujio Yu , Tetsuji Nakamura
摘要： The present invention provides: a protein having an improved nitrile hydratase activity, whereby heat resistance has been improved when compared with a wild-type nitrile hydratase activity, wherein the amino acid sequence of a nitrile hydratase is modified; a gene DNA encoding the above protein; a recombinant vector having the above gene DNA; a transformant or transductant having the above recombinant vector; a nitrile hydratase collected from a culture of the above transformant or transductant, and a production method thereof; and a method for producing an amide compound.
摘要翻译： 本发明提供：腈水合酶活性提高的蛋白质，与野生型腈水合酶活性相比，耐水性得到改善，其中腈水合酶的氨基酸序列被修饰; 编码上述蛋白质的基因DNA; 具有上述基因DNA的重组载体; 具有上述重组载体的转化体或转导体; 从上述转化体或转导体的培养物收集的腈水合酶及其制备方法; 和酰胺化合物的制造方法。
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公开(公告)号：US20070231868A1
公开(公告)日：2007-10-04
申请号：US11569529
申请日：2005-05-26
申请人： Fumiaki Watanabe , Dai Ujihara , Miki Sakai , Fujio Yu , Tetsuji Nakamura
发明人： Fumiaki Watanabe , Dai Ujihara , Miki Sakai , Fujio Yu , Tetsuji Nakamura
摘要： The present invention provides: a protein having an improved nitrile hydratase activity, whereby heat resistance has been improved when compared with a wild-type nitrile hydratase activity, wherein the amino acid sequence of a nitrile hydratase is modified; a gene DNA encoding the above protein; a recombinant vector having the above gene DNA; a transformant or transductant having the above recombinant vector; a nitrile hydratase collected from a culture of the above transformant or transductant, and a production method thereof; and a method for producing an amide compound.
摘要翻译： 本发明提供：腈水合酶活性提高的蛋白质，与野生型腈水合酶活性相比，耐水性得到改善，其中腈水合酶的氨基酸序列被修饰; 编码上述蛋白质的基因DNA; 具有上述基因DNA的重组载体; 具有上述重组载体的转化体或转导体; 从上述转化体或转导体的培养物收集的腈水合酶及其制备方法; 和酰胺化合物的制造方法。
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公开(公告)号：US06617139B1
公开(公告)日：2003-09-09
申请号：US09958969
申请日：2001-10-16
申请人： Tetsuji Nakamura , Fujio Yu
发明人： Tetsuji Nakamura , Fujio Yu
IPC分类号： C12P1304
摘要： The present invention relates to a novel protein having amidase activity that stereoselectively hydrolyzes &agr;-amino acid amides and &agr;-hydroxy acid amides, and a gene encoding said protein.
摘要翻译： 本发明涉及具有立体选择性水解α-氨基酸酰胺和α-羟基酰胺的酰胺酶活性的新型蛋白质，以及编码所述蛋白质的基因。
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8.发明授权DNA fragments containing gene having function relating to autonomous proliferation of plasmid 有权含有与质粒自主增殖有关的功能基因的DNA片段公开(公告)号：US07557202B2
公开(公告)日：2009-07-07
申请号：US10482156
申请日：2002-07-03
申请人： Wataru Mizunashi , Fujio Yu
发明人： Wataru Mizunashi , Fujio Yu
CPC分类号： C07K14/195 , C12N15/69
摘要： Provided are a DNA fragment containing a gene relating the autonomous replication of a plasmid, and a DNA fragment containing a gene relating to the autonomous replication of a plasmid wherein a mutation point capable of increasing the number of copies of a plasmid is present in at least one site in the gene. Also provided are a DNA fragment containing a gene having a function relating to the autonomous replication of a plasmid within the bacteria of the genus Rhodococcus, a DNA fragment wherein a mutation point capable of increasing the number of copies of a plasmid is present in at least one site in the gene, and a plasmid carrying a DNA fragment wherein a mutation point capable of increasing the number of copies of a plasmid is present in at least one site of the gene.
摘要翻译： 提供了含有与质粒自主复制有关的基因的DNA片段和含有与质粒自主复制有关的基因的DNA片段，其中能够增加质粒拷贝数的突变点至少存在 基因中的一个位点。 还提供了含有与红球菌属细菌中的质粒自主复制有关的功能的基因的DNA片段，其中至少存在能增加质粒拷贝数的突变点的DNA片段 该基因的一个位点和携带DNA片段的质粒，其中能够增加质粒拷贝数的突变点存在于该基因的至少一个位点。
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9.发明授权Kanamycin resistance gene derived from microorganisms of the genus rhodococcus 失效源自红球菌属微生物的卡那霉素抗性基因
公开(公告)号：US5776771A
公开(公告)日：1998-07-07
申请号：US510878
申请日：1995-08-03
IPC分类号： C12N15/09 , C07K14/195 , C07K14/41 , C12N1/21 , C12N9/00 , C12N15/65 , C12N15/74 , C12P21/02 , C12R1/01 , C12R1/19 , C12N15/31 , C12N15/70
CPC分类号： C12N15/65 , C07K14/195 , C12N15/74 , C12N9/00
摘要： The present invention relates to a DNA derived from microorganisms of the genus Rhodococcus and conferring kanamycin resistance on hosts with a DNA sequence coding for the amino acid sequence of Sequence No. 1 or a polypeptide containing a partial sequence thereof. The kanamycin resistance gene of the present invention is useful to construct vectors for microorganisms of the genus Rhodococcus, particularly vectors for self-cloning of Rhodococcus rhodochrous.
摘要翻译： 本发明涉及一种衍生自红球菌属微生物的DNA，并赋予具有编码序列号1的氨基酸序列的DNA序列的宿主的卡那霉素抗性或含有其部分序列的多肽。 本发明的卡那霉素抗性基因可用于构建红球菌属微生物的载体，特别是用于玫瑰色红球菌自身克隆的载体。
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公开(公告)号：US5602014A
公开(公告)日：1997-02-11
申请号：US577184
申请日：1995-12-22
申请人： Yurie Mizumura , Fujio Yu
发明人： Yurie Mizumura , Fujio Yu
IPC分类号： C12N15/09 , C07K14/195 , C12N1/21 , C12N9/78 , C12N15/31 , C12N15/74 , C12R1/01 , C12N15/00 , C12P21/00
CPC分类号： C12N9/78 , C07K14/195 , C12N15/74
摘要： The invention relates to a regulatory factor or system composed of 2 components of a polypeptide having the amino acid sequence of SEQ ID No:1 and a polypeptide having the amino acid sequence of SEQ ID No:2 to activate a nitrilase gene promoter, as well as to DNA coding therefor. Nitrilase can be produced by introducing the gene coding for the present regulatory factor together with a nitrilase gene containing a promoter region into a microorganism of the genus Rhodococcus.
摘要翻译： 本发明涉及由具有SEQ ID No：1的氨基酸序列的多肽的2种成分和具有SEQ ID No：2的氨基酸序列的多肽组成的调节因子或系统，以激活腈水解酶基因启动子 关于DNA编码。 可以通过将编码本调节因子的基因与含有启动子区域的腈水解酶基因导入红球菌属的微生物来生产腈水解酶。
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