利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

11 条记录 (耗时 0.012 秒)

    Purification and recovery of neutral and alkaline protease using cationic sulfonated phenol-formaldehyde resin
    2.
    发明授权
    Purification and recovery of neutral and alkaline protease using cationic sulfonated phenol-formaldehyde resin 失效
    使用阳离子磺化酚醛树脂的中性和碱性蛋白酶的纯化和回收

    公开(公告)号：US3592738A

    公开(公告)日：1971-07-13

    申请号：US3592738D

    申请日：1968-08-14

    申请人： MONSANTO CO

    发明人： KEAY LEONARD

    IPC分类号： C12N9/56 ,  C07G7/02

    CPC分类号： C12N9/54 ,  Y10S435/839 ,  Y10S435/844

    摘要： AQUEOUS SOLUTION OF PROTEASE, E.G., A CLARIFIED BEER FROM B. SUBTILIS MICROORGANISM PRODUCTION OF ENZYMES OR REDISSOLVED ENZYME MIXTURE, IS TREATED WITH SULFONATED PHENOLFORMALDEHYDE CATIONIC EXCHANGE RESIN TO ELIMINATE UNDESIRABLE COLORED MATERIALS AND ANY AMYLASE PRESENT. PROTEASE IS ADSORBED: AMYLASE AND MOST PIGMENTED IMPURITIES ARE NOT. NEUTRAL PROTEASE CAN BE RECOVERED OR ALKALINE PROTEASE CAN BE RECOVERED; BOTH CAN BE RECOVERED TOGEHTER OR WITH FRACTIONATION WHEN BOTH ARE PRESENT. USE OF NEUTRAL PH SOLUTIONS FOR ELUTION ELIMINATES PIGMENT ELUTION PROBLEMS, AND USE OF LOW IONIC STRENGTH SOLUTIONS IN PREPARATION OF RESIN AND FOR ADSORPTIONALLOWS RECOVERY OF MAXIMUM RANGE OF PROTEASES. FRACTIONATION IS EFFECTED BY USE OF MODERATE IONIC STRENGTH SOLUTION FOR ELUTION OF NEUTRAL PROTEASE AND HIGHER IONIC STRENGTH SOLUTION FOR ALKALINE PROTEASE. WHERE FRACTIONATION IS NOT REQUIRED, ELUTION MAY BE WITH HIGH IONIC STRENGTH SOLUTION.

    Process for the production of R(-)-4-halo-3-hydroxybutyronitrile
    6.
    发明授权
    Process for the production of R(-)-4-halo-3-hydroxybutyronitrile 失效
    制备R（ - ） - 4-卤代-3-羟基丁腈的方法

    公开(公告)号：US5210031A

    公开(公告)日：1993-05-11

    申请号：US830516

    申请日：1992-02-03

    申请人： Tetsuji Nakamura ,  Fujio Yu

    发明人： Tetsuji Nakamura ,  Fujio Yu

    IPC分类号： C12P13/00

    CPC分类号： C12P13/002 ,  Y10S435/822 ,  Y10S435/843 ,  Y10S435/844 ,  Y10S435/845 ,  Y10S435/846

    摘要： A process for the production of a 4-halo-3-hydroxybutyronitrile which comprises reacting an epihalohydrin with a halohydrin-halide-lyase originating from a microorganism selected from the group consisting of: Corynebacterium sp. N-2354 FERM BP-2726 and Microbacterium sp. N-4701 FERM BP-2644 in the presence of an alkali cyanide to thereby convert the epihalohydrin into the 4-halo-3-hydroxybutyronitrile and collecting the product thus formed is disclosed. According to this process, a 4-halo-3-hydroxybutyronitrile which is highly useful in the syntheses of various medicines and physiologically active substances can be easily and efficiently produced from an inexpensive starting material.

    摘要翻译： 一种制备4-卤代-3-羟基丁腈的方法，其包括使表卤代醇与源自选自以下的微生物的卤代醇 - 卤化物裂解酶反应：棒状杆菌属 N-2354 FERM BP-2726和Microbacterium sp。 N-4701 FERM BP-2644在碱金属氰化物的存在下，从而将表卤代醇转化成4-卤代-3-羟基丁腈并收集如此形成的产品。 根据该方法，可以从廉价的原料容易且有效地制备在各种药物和生理活性物质的合成中非常有用的4-卤代-3-羟基丁腈。

    Process for producing proteins correlated with the diphtheric toxin
    7.
    发明授权
    Process for producing proteins correlated with the diphtheric toxin 失效
    与蛋白质相关的蛋白质生产过程

    公开(公告)号：US4925792A

    公开(公告)日：1990-05-15

    申请号：US575335

    申请日：1984-01-31

    申请人： Rino Rappuoli

    发明人： Rino Rappuoli

    IPC分类号： C12N15/09 ,  A61K39/05 ,  C07K14/34 ,  C12N15/00 ,  C12N15/12 ,  C12P21/00 ,  C12R1/16

    CPC分类号： C12R1/16 ,  C07K14/34 ,  Y10S435/844

    摘要： Process for the production of a protein correlated with the diphtheric toxin which comprises culturing in a liquid nutrient medium having a concentration of iron ions of from 0.05 .mu.g/ml to 0.5 .mu.g/ml, at a temperature of from 30.degree. C. to 40.degree. C., in a neutral culturing environment and under aerobic conditions, a microorganism belonging to the Corynebacterium diphtheriae genus with two mutant phages encoding the protein correlated with the diphtheric toxin integrated in a nontandem mode in their chromosomes.

    摘要翻译： 用于生产与嗜热毒素相关的蛋白质的方法，其包括在铁离子浓度为0.05μg/ ml至0.5μg/ ml的液体营养培养基中，在30℃至 40℃，在中性培养环境中，在需氧条件下，属于白喉棒状杆菌属的微生物，其两个突变噬菌体编码蛋白质，与染色体中以非串联模式集成的二倍体毒素相关。

    Device and method for testing potency of biological control reagents
    8.
    发明授权
    Device and method for testing potency of biological control reagents 失效
    用于测试生物控制试剂质量的装置和方法

    公开(公告)号：US3713985A

    公开(公告)日：1973-01-30

    申请号：US3713985D

    申请日：1970-10-19

    申请人： KANTOR F

    发明人： ASTLE T

    IPC分类号： B01L3/00 ,  B01L3/14 ,  C12M1/20 ,  C12K1/04

    CPC分类号： B01L3/5085 ,  B01L3/50855 ,  B01L3/5453 ,  Y10S435/81 ,  Y10S435/844 ,  Y10S435/975

    摘要： Device for testing the potency of a biological control reagent, as in antibiotic susceptibility testing, especially the determination of minimum inhibitory concentration, comprising a strip of a plurality of interconnected plastic cups having a capacity of about 0.3 ml. each. All but two of the cups contain a range of amounts of the control reagent in an essentially dry state which will provide a predetermined range of concentrations when the reagent is redispersed. Contamination of the dry reagent in the cups is prevented by an adherent plastic film or aluminum foil across the openings of the cups. In use, the adherent film or foil is removed, the dry reagent is redispersed to form the required range of concentrations, and all of the cups except one are inoculated with the biological material against which the potency of the control reagent is to be tested. The last cup contains the dispersion medium but not the control reagent nor inoculum, and provides a sterility control. The second last cup contains the dispersion medium and the inoculum, and serves as a growth control. The strip of cups is then resealed, incubated, and the test results are read.

    摘要翻译： 用于测试生物控制试剂的效力的装置，如在抗生素敏感性试验中，特别是最小抑制浓度的测定，其包含容量为约0.3ml的多个相互连接的塑料杯条。 每。 除了两个杯子之外，除了两个杯子之外还包含一定量的基本干燥状态的对照试剂，当试剂再分散时，其将提供预定范围的浓度。 通过在杯子的开口上的粘附塑料膜或铝箔来防止杯子中干试剂的污染。 在使用中，去除粘附膜或箔，干燥的试剂再分散以形成所需的浓度范围，除了一种以外的所有杯子都接种用于测试对照试剂的效力的生物材料。 最后一杯含有分散介质，但不含对照试剂和接种物，并提供无菌控制。 第二杯含有分散介质和接种物，并用作生长控制。 然后将杯条重新密封，孵育，并读取测试结果。