利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

26 条记录 (耗时 0.033 秒)

    Nitrile Hydratase
    2.
    发明申请
    Nitrile Hydratase 有权
    腈水合酶

    公开(公告)号：US20070231868A1

    公开(公告)日：2007-10-04

    申请号：US11569529

    申请日：2005-05-26

    申请人： Fumiaki Watanabe ,  Dai Ujihara ,  Miki Sakai ,  Fujio Yu ,  Tetsuji Nakamura

    发明人： Fumiaki Watanabe ,  Dai Ujihara ,  Miki Sakai ,  Fujio Yu ,  Tetsuji Nakamura

    IPC分类号： C12P13/02 ,  C07H21/00 ,  C07K14/00 ,  C12N1/00 ,  C12N15/00 ,  C12N9/88

    CPC分类号： C12P13/02 ,  C12N9/88

    摘要： The present invention provides: a protein having an improved nitrile hydratase activity, whereby heat resistance has been improved when compared with a wild-type nitrile hydratase activity, wherein the amino acid sequence of a nitrile hydratase is modified; a gene DNA encoding the above protein; a recombinant vector having the above gene DNA; a transformant or transductant having the above recombinant vector; a nitrile hydratase collected from a culture of the above transformant or transductant, and a production method thereof; and a method for producing an amide compound.

    摘要翻译： 本发明提供：腈水合酶活性提高的蛋白质，与野生型腈水合酶活性相比，耐水性得到改善，其中腈水合酶的氨基酸序列被修饰; 编码上述蛋白质的基因DNA; 具有上述基因DNA的重组载体; 具有上述重组载体的转化体或转导体; 从上述转化体或转导体的培养物收集的腈水合酶及其制备方法; 和酰胺化合物的制造方法。

    Heat-resistant nitrile hydratase
    3.
    发明授权
    Heat-resistant nitrile hydratase 有权
    耐热腈水合酶

    公开(公告)号：US07803578B2

    公开(公告)日：2010-09-28

    申请号：US11947955

    申请日：2007-11-30

    申请人： Fumiaki Watanabe ,  Dai Ujihara ,  Miki Sakai ,  Fujio Yu ,  Tetsuji Nakamura

    发明人： Fumiaki Watanabe ,  Dai Ujihara ,  Miki Sakai ,  Fujio Yu ,  Tetsuji Nakamura

    IPC分类号： C12P21/06 ,  C12N9/00 ,  C12N9/88 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

    CPC分类号： C12P13/02 ,  C12N9/88

    摘要： The present invention provides: a protein having an improved nitrile hydratase activity, whereby heat resistance has been improved when compared with a wild-type nitrile hydratase activity, wherein the amino acid sequence of a nitrile hydratase is modified; a gene DNA encoding the above protein; a recombinant vector having the above gene DNA; a transformant or transductant having the above recombinant vector; a nitrile hydratase collected from a culture of the above transformant or transductant, and a production method thereof; and a method for producing an amide compound.

    摘要翻译： 本发明提供：腈水合酶活性提高的蛋白质，与野生型腈水合酶活性相比，耐水性得到改善，其中腈水合酶的氨基酸序列被修饰; 编码上述蛋白质的基因DNA; 具有上述基因DNA的重组载体; 具有上述重组载体的转化体或转导体; 从上述转化体或转导体的培养物收集的腈水合酶及其制备方法; 和酰胺化合物的制造方法。

    NITRILE HYDRATASE
    4.
    发明申请
    NITRILE HYDRATASE 有权

    公开(公告)号：US20090162894A1

    公开(公告)日：2009-06-25

    申请号：US11947955

    申请日：2007-11-30

    申请人： Fumiaki Watanabe ,  Dai Ujihara ,  Miki Sakai ,  Fujio Yu ,  Tetsuji Nakamura

    发明人： Fumiaki Watanabe ,  Dai Ujihara ,  Miki Sakai ,  Fujio Yu ,  Tetsuji Nakamura

    IPC分类号： C07H21/04 ,  C12N15/63 ,  C12N1/21 ,  C12P21/00

    CPC分类号： C12P13/02 ,  C12N9/88

    摘要： The present invention provides: a protein having an improved nitrile hydratase activity, whereby heat resistance has been improved when compared with a wild-type nitrile hydratase activity, wherein the amino acid sequence of a nitrile hydratase is modified; a gene DNA encoding the above protein; a recombinant vector having the above gene DNA; a transformant or transductant having the above recombinant vector; a nitrile hydratase collected from a culture of the above transformant or transductant, and a production method thereof; and a method for producing an amide compound.

    摘要翻译： 本发明提供：腈水合酶活性提高的蛋白质，与野生型腈水合酶活性相比，耐水性得到改善，其中腈水合酶的氨基酸序列被修饰; 编码上述蛋白质的基因DNA; 具有上述基因DNA的重组载体; 具有上述重组载体的转化体或转导体; 从上述转化体或转导体的培养物收集的腈水合酶及其制备方法; 和酰胺化合物的制造方法。

    HALOHYDRIN EPOXIDASE
    5.
    发明申请
    HALOHYDRIN EPOXIDASE 失效
    盐酸环氧化酶

    公开(公告)号：US20100136617A1

    公开(公告)日：2010-06-03

    申请号：US12529657

    申请日：2008-03-07

    申请人： Takanori Akiyama ,  Fujio Yu ,  Fumiaki Watanabe ,  Yuya Takikawa ,  Eiji Sato

    发明人： Takanori Akiyama ,  Fujio Yu ,  Fumiaki Watanabe ,  Yuya Takikawa ,  Eiji Sato

    IPC分类号： C12P21/06 ,  C12N9/88 ,  C07H21/04 ,  C12N15/63 ,  C12N5/10 ,  C12P17/02 ,  C12P13/00

    CPC分类号： C12P13/004 ,  C12N9/0071

    摘要： The present invention provides an industrially useful improved halohydrin epoxidase, a method for producing the same, and a method for producing an epihalohydrin or 4-halo-3-hydroxybutyronitrile using the same. The improved halohydrin epoxidase of the present invention consists of an amino acid sequence in which a specific amino acid substitution mutation is introduced into an amino acid sequence of a wild-type halohydrin epoxidase comprising predetermined amino acid sequences I and II.

    摘要翻译： 本发明提供工业上有用的改进的卤代醇环氧酶，其制备方法，以及使用其制备表卤代醇或4-卤代-3-羟基丁腈的方法。 本发明的改进的卤代醇环氧酶由将特定氨基酸取代突变引入包含预定氨基酸序列I和II的野生型卤代醇环氧酶的氨基酸序列中的氨基酸序列组成。

    Process for producing vinylated nucleic acid
    6.
    发明申请
    Process for producing vinylated nucleic acid 审中-公开
    生产乙烯基化核酸的方法

    公开(公告)号：US20060252086A1

    公开(公告)日：2006-11-09

    申请号：US11438463

    申请日：2006-05-23

    申请人： Toshitaka Uragaki ,  Fumiaki Watanabe ,  Chiaki Nagahama ,  Fujio Yu ,  Katsuaki Kikuchi ,  Akira Yoshioka

    发明人： Toshitaka Uragaki ,  Fumiaki Watanabe ,  Chiaki Nagahama ,  Fujio Yu ,  Katsuaki Kikuchi ,  Akira Yoshioka

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

    CPC分类号： C07H21/00

    摘要： This invention provides a process for producing a vinylated nucleic acid in an efficient and cost-effective manner through the implementation of vinylation of an amino-containing nucleic acid with a vinylating agent. This invention also provides a process for producing a vinylated nucleic acid in an efficient and cost-effective manner by a method in which PCR is carried out in the presence of an amino-modified nucleotide and dNTP and a vinyl group is introduced into the resulting PCR amplification product or by a method in which PCR is carried out in the presence of a vinyl-modified nucleotide and cNTP.

    摘要翻译： 本发明提供了一种通过使用乙烯化剂实施含氨基核酸的乙烯化作用，以有效且成本有效的方式生产乙烯基化核酸的方法。 本发明还提供了通过在氨基修饰的核苷酸和dNTP的存在下进行PCR并将乙烯基引入到所得PCR中的方法，以有效和成本有效的方式制备乙烯基化核酸的方法 扩增产物或通过在乙烯基修饰的核苷酸和cNTP存在下进行PCR的方法。

    Process for the production of R(-)-4-halo-3-hydroxybutyronitrile
    7.
    发明授权
    Process for the production of R(-)-4-halo-3-hydroxybutyronitrile 失效
    制备R（ - ） - 4-卤代-3-羟基丁腈的方法

    公开(公告)号：US5210031A

    公开(公告)日：1993-05-11

    申请号：US830516

    申请日：1992-02-03

    申请人： Tetsuji Nakamura ,  Fujio Yu

    发明人： Tetsuji Nakamura ,  Fujio Yu

    IPC分类号： C12P13/00

    CPC分类号： C12P13/002 ,  Y10S435/822 ,  Y10S435/843 ,  Y10S435/844 ,  Y10S435/845 ,  Y10S435/846

    摘要： A process for the production of a 4-halo-3-hydroxybutyronitrile which comprises reacting an epihalohydrin with a halohydrin-halide-lyase originating from a microorganism selected from the group consisting of: Corynebacterium sp. N-2354 FERM BP-2726 and Microbacterium sp. N-4701 FERM BP-2644 in the presence of an alkali cyanide to thereby convert the epihalohydrin into the 4-halo-3-hydroxybutyronitrile and collecting the product thus formed is disclosed. According to this process, a 4-halo-3-hydroxybutyronitrile which is highly useful in the syntheses of various medicines and physiologically active substances can be easily and efficiently produced from an inexpensive starting material.

    摘要翻译： 一种制备4-卤代-3-羟基丁腈的方法，其包括使表卤代醇与源自选自以下的微生物的卤代醇 - 卤化物裂解酶反应：棒状杆菌属 N-2354 FERM BP-2726和Microbacterium sp。 N-4701 FERM BP-2644在碱金属氰化物的存在下，从而将表卤代醇转化成4-卤代-3-羟基丁腈并收集如此形成的产品。 根据该方法，可以从廉价的原料容易且有效地制备在各种药物和生理活性物质的合成中非常有用的4-卤代-3-羟基丁腈。

    Amidase gene
    10.
    发明授权
    Amidase gene 失效
    酰胺酶基因

    公开(公告)号：US06617139B1

    公开(公告)日：2003-09-09

    申请号：US09958969

    申请日：2001-10-16

    申请人： Tetsuji Nakamura ,  Fujio Yu

    发明人： Tetsuji Nakamura ,  Fujio Yu

    IPC分类号： C12P1304

    CPC分类号： C12N9/80 ,  C12P13/04

    摘要： The present invention relates to a novel protein having amidase activity that stereoselectively hydrolyzes &agr;-amino acid amides and &agr;-hydroxy acid amides, and a gene encoding said protein.

    摘要翻译： 本发明涉及具有立体选择性水解α-氨基酸酰胺和α-羟基酰胺的酰胺酶活性的新型蛋白质，以及编码所述蛋白质的基因。