公开(公告)号：US20190241608A1

公开(公告)日：2019-08-08

申请号：US16386628

申请日：2019-04-17

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Jean-Luc Maloisel ,  Ola Lind ,  Bjorn Noren ,  Ronnie Palmgren

IPC: C07K1/22 ,  C07K16/06 ,  C07K16/00

CPC classification number: C07K1/22 ,  C07K1/145 ,  C07K16/00 ,  C07K16/065 ,  C07K2317/55

Abstract: The present invention relates to a method for removal of large contaminants, such as virus, in a chromatographic process for purification of a target molecule, preferably monoclonal antibodies, mAbs, by using a specifically designed chromatographic bead having a thin outer layer and a core functionalized with a ligand adsorbing the mAbs or parts thereof.

公开(公告)号：US10465153B2

公开(公告)日：2019-11-05

申请号：US15037539

申请日：2014-11-26

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Jean-Luc Maloisel ,  Tobias Soderman ,  Ola Lind

IPC: C12H1/056 ,  B01D15/00 ,  B01J20/32 ,  B01J20/28 ,  C08F251/00

Abstract: The invention discloses a separation matrix with a porous solid support and a plurality of polyvinylpyrrolidone (PVP) polymer chains covalently attached to the solid support. The polyvinylpyrrolidone polymer chains are either vinylpyrrolidone homopolymer chains or copolymer chains which comprise at least 70 mol % vinylpyrrolidone monomer residues and less than 2 mol % negatively charged monomer residues.

公开(公告)号：US20190111419A1

公开(公告)日：2019-04-18

申请号：US16090609

申请日：2017-03-30

Applicant: GE HEALTHCARE BIOPROCESS R&D AB

Inventor： Jean-Luc Maloisel ,  Tobias Söderman ,  Ola Lind ,  Ida Eklind

IPC: B01J47/02 ,  B01J39/07 ,  B01J39/19 ,  B01J39/26 ,  B01J41/07 ,  B01J41/13 ,  B01J41/20 ,  C07K1/18 ,  B01D15/36 ,  B01D15/38 ,  B01D15/32

Abstract: The invention discloses a separation matrix for purification of biomacromolecules, comprising a plurality of particles (1) having a core region (2) and a shell region (3), wherein: a) said shell region is accessible to a target biomacromolecule; b) said core region is less accessible to the target biomacromolecule than the shell region; and c) the core region comprises a grafted polymer comprising residues of at least one polymerizable monomer.

公开(公告)号：US10266563B2

公开(公告)日：2019-04-23

申请号：US15308327

申请日：2015-06-04

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Jean-Luc Maloisel ,  Ola Lind ,  Bjorn Noren ,  Ronnie Palmgren

IPC: C07K1/22 ,  C07K16/00 ,  C07K16/06 ,  C07K1/14

Abstract: The present invention relates to a method for removal of large contaminants, such as virus, in a chromatographic process for purification of a target molecule, preferably monoclonal antibodies, mAbs, by using a specifically designed chromatographic bead having a thin outer layer and a core functionalized with a ligand adsorbing the mAbs or parts thereof.

公开(公告)号：US20170051010A1

公开(公告)日：2017-02-23

申请号：US15308327

申请日：2015-06-04

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Jean-Luc Maloisel ,  Ola Lind ,  Bjorn Noren ,  Ronnie Palmgren

IPC: C07K1/22 ,  C07K16/00

CPC classification number: C07K1/22 ,  C07K1/145 ,  C07K16/00 ,  C07K16/065 ,  C07K2317/55

Abstract: The present invention relates to a method for removal of large contaminants, such as virus, in a chromatographic process for purification of a target molecule, preferably monoclonal antibodies, mAbs, by using a specifically designed chromatographic bead having a thin outer layer and a core functionalized with a ligand adsorbing the mAbs or parts thereof.

Abstract translation: 本发明涉及在用于纯化目标分子的色谱方法（优选单克隆抗体mAb）中通过使用具有薄的外层和核心功能化的专门设计的色谱珠来去除大污染物如病毒的方法 具有吸附mAb或其部分的配体。

公开(公告)号：US20160298065A1

公开(公告)日：2016-10-13

申请号：US15037539

申请日：2014-11-26

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Jean-Luc Maloisel ,  Tobias Soderman ,  Ola Lind

IPC: C12H1/056 ,  B01J20/32 ,  B01J20/28 ,  C08F251/00

CPC classification number: C12H1/0424 ,  B01D15/00 ,  B01J20/28004 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/327 ,  B01J20/3278 ,  B01J20/3293 ,  B01J2220/58 ,  C08F251/00

Abstract: The invention discloses a separation matrix with a porous solid support and a plurality of polyvinylpyrrolidone (PVP) polymer chains covalently attached to the solid support. The polyvinylpyrrolidone polymer chains are either vinylpyrrolidone homopolymer chains or copolymer chains which comprise at least 70 mol % vinylpyrrolidone monomer residues and less than 2 mol % negatively charged monomer residues.

Abstract translation: 本发明公开了具有多孔固体支持物和共价连接到固体支持物上的多个聚乙烯吡咯烷酮（PVP）聚合物链的分离基质。 聚乙烯吡咯烷酮聚合物链是乙烯基吡咯烷酮均聚物链或包含至少70mol％乙烯基吡咯烷酮单体残基和小于2mol％带负电荷的单体残基的共聚物链。