公开(公告)号：US10730908B2

公开(公告)日：2020-08-04

申请号：US15798784

申请日：2017-10-31

Applicant: GE HEALTHCARE BIOPROCESS R&D AB

Inventor： Annika Forss ,  Mats Ander ,  Tomas Bjorkman ,  Hans Blom ,  Jesper Hansson ,  Gustav Rodrigo

IPC: C07K1/16 ,  B01D15/08 ,  C07K1/22 ,  C07K16/00 ,  B01J20/286 ,  B01J20/285 ,  B01D15/18 ,  B01D15/34 ,  C07K14/31

Abstract: The invention relates to a method of isolating an immunoglobulin, comprising the steps of: a) providing a separation matrix comprising at least 15 mg/ml multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, wherein the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml; b) contacting a liquid sample comprising an immunoglobulin with the separation matrix; c) washing the separation matrix with a washing liquid; d) eluting the immunoglobulin from the separation matrix with an elution liquid; and e) cleaning the separation matrix with a cleaning liquid comprising at least 0.5 M NaOH.

公开(公告)号：US10124328B2

公开(公告)日：2018-11-13

申请号：US15407240

申请日：2017-01-16

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Jesper Hansson ,  Gustav Rodrigo ,  Tobias E Soderman

IPC: B01J39/26 ,  B01D15/18 ,  B01D15/36 ,  B01J20/286 ,  B01J20/32 ,  B01D15/38 ,  B01J20/26 ,  B01J20/28 ,  B01J20/289 ,  B01J20/30 ,  C07H1/08 ,  C07K1/22 ,  B01D15/34 ,  B01J20/24 ,  G01N30/88

Abstract: The invention discloses a method of separating a biomolecule from at least one other component in a liquid, comprising a step of contacting said liquid with a separation matrix comprising a solid support and polymer chains bound to said solid support. The polymer chains comprise units derived from a first monomer of structure CH2═CH-L-X, where L is a covalent bond or an alkyl ether or hydroxysubstituted alkyl ether chain comprising 2-6 carbon atoms, and X is a sulfonate or phosphonate group.

公开(公告)号：US20180044374A1

公开(公告)日：2018-02-15

申请号：US15798784

申请日：2017-10-31

Applicant: GE HEALTHCARE BIOPROCESS R&D AB

Inventor： Annika Forss ,  Mats Ander ,  Tomas Bjorkman ,  Hans Blom ,  Jesper Hansson ,  Gustav Rodrigo

IPC: C07K1/16 ,  B01D15/18 ,  B01J20/285 ,  B01D15/34 ,  C07K16/00 ,  B01J20/286

CPC classification number: C07K1/165 ,  B01D15/1807 ,  B01D15/1864 ,  B01D15/34 ,  B01J20/285 ,  B01J20/286 ,  C07K1/22 ,  C07K14/31 ,  C07K16/00 ,  C07K2317/21 ,  C07K2317/31 ,  C07K2319/30

Abstract: The invention relates to a method of isolating an immunoglobulin, comprising the steps of: a) providing a separation matrix comprising at least 15 mg/ml multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, wherein the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml; b) contacting a liquid sample comprising an immunoglobulin with the separation matrix; c) washing the separation matrix with a washing liquid; d) eluting the immunoglobulin from the separation matrix with an elution liquid; and e) cleaning the separation matrix with a cleaning liquid comprising at least 0.5 M NaOH.

公开(公告)号：US09573973B2

公开(公告)日：2017-02-21

申请号：US14396207

申请日：2013-04-22

Applicant: GE HEALTHCARE BIOPROCESS R&D AB

Inventor： Jesper Hansson ,  Gustav Rodrigo ,  Tobias E Soderman

IPC: B01D15/36 ,  C07K1/22 ,  B01D15/18 ,  B01J20/286 ,  B01J20/32 ,  B01D15/38 ,  B01J20/26 ,  B01J20/28 ,  B01J20/289 ,  B01J20/30 ,  C07H1/08 ,  G01N30/88

CPC classification number: B01J39/26 ,  B01D15/1864 ,  B01D15/34 ,  B01D15/361 ,  B01D15/362 ,  B01D15/3809 ,  B01J20/24 ,  B01J20/261 ,  B01J20/265 ,  B01J20/267 ,  B01J20/28014 ,  B01J20/28033 ,  B01J20/286 ,  B01J20/289 ,  B01J20/3085 ,  B01J20/3212 ,  B01J20/3278 ,  B01J20/3282 ,  B01J2220/80 ,  C07H1/08 ,  C07K1/22 ,  G01N2030/8827 ,  Y02P20/582

Abstract: The invention discloses a method of separating a biomolecule from at least one other component in a liquid, comprising a step of contacting said liquid with a separation matrix comprising a solid support and polymer chains bound to said solid support. The polymer chains comprise units derived from a first monomer of structure CH2═CH-L-X, where L is a covalent bond or an alkyl ether or hydroxysubstituted alkyl ether chain comprising 2-6 carbon atoms, and X is a sulfonate or phosphonate group.

Abstract translation: 本发明公开了一种从液体中的至少一种其它组分分离生物分子的方法，包括使所述液体与包含固体支持物和与所述固体支持物结合的聚合物链的分离基质接触的步骤。 聚合物链包含衍生自结构为CH 2 = CH-L-X的第一单体的单元，其中L为共价键或包含2-6个碳原子的烷基醚或羟基取代的烷基醚链，X为磺酸盐或膦酸酯基团。

公开(公告)号：US20200317725A1

公开(公告)日：2020-10-08

申请号：US16911511

申请日：2020-06-25

Applicant: GE HEALTHCARE BIOPROCESS R&D AB

Inventor： Annika Forss ,  Mats Ander ,  Tomas Bjorkman ,  Hans Blom ,  Jesper Hansson ,  Gustav Rodrigo

IPC: C07K1/16 ,  C07K16/00 ,  B01J20/286 ,  B01J20/285 ,  B01D15/18 ,  B01D15/34 ,  C07K1/22 ,  C07K14/31

Abstract: The invention relates to a method of isolating an immunoglobulin, comprising the steps of: a) providing a separation matrix comprising at least 15 mg/ml multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, wherein the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml; b) contacting a liquid sample comprising an immunoglobulin with the separation matrix; c) washing the separation matrix with a washing liquid; d) eluting the immunoglobulin from the separation matrix with an elution liquid; and e) cleaning the separation matrix with a cleaning liquid comprising at least 0.5 M NaOH.

公开(公告)号：US20170120232A1

公开(公告)日：2017-05-04

申请号：US15407240

申请日：2017-01-16

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Jesper Hansson ,  Gustav Rodrigo ,  Tobias E. Soderman

IPC: B01J39/26 ,  B01J20/24 ,  B01D15/36 ,  C07K1/22 ,  B01D15/38 ,  B01D15/34 ,  B01J20/26 ,  B01J20/286

CPC classification number: B01J39/26 ,  B01D15/1864 ,  B01D15/34 ,  B01D15/361 ,  B01D15/362 ,  B01D15/3809 ,  B01J20/24 ,  B01J20/261 ,  B01J20/265 ,  B01J20/267 ,  B01J20/28014 ,  B01J20/28033 ,  B01J20/286 ,  B01J20/289 ,  B01J20/3085 ,  B01J20/3212 ,  B01J20/3278 ,  B01J20/3282 ,  B01J2220/80 ,  C07H1/08 ,  C07K1/22 ,  G01N2030/8827 ,  Y02P20/582

Abstract: The invention discloses a method of separating a biomolecule from at least one other component in a liquid, comprising a step of contacting said liquid with a separation matrix comprising a solid support and polymer chains bound to said solid support. The polymer chains comprise units derived from a first monomer of structure CH2═CH-L-X, where L is a covalent bond or an alkyl ether or hydroxysubstituted alkyl ether chain comprising 2-6 carbon atoms, and X is a sulfonate or phosphonate group.