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    Purified hepatitis C virus envelope proteins for diagnostic and therapeutic use
    2.
    发明授权
    Purified hepatitis C virus envelope proteins for diagnostic and therapeutic use 失效
    纯化的丙型肝炎病毒包膜蛋白用于诊断和治疗用途

    公开(公告)号：US06890737B1

    公开(公告)日：2005-05-10

    申请号：US08928757

    申请日：1997-09-12

    申请人： Geert Maertens ,  Fons Bosman ,  Guy De Martynoff ,  Marie-Ange Buyse

    发明人： Geert Maertens ,  Fons Bosman ,  Guy De Martynoff ,  Marie-Ange Buyse

    IPC分类号： A61K39/00 ,  C07K14/18 ,  C12P21/06 ,  A61K39/12 ,  C07K1/00

    CPC分类号： C07K14/005 ,  A61K39/00 ,  C12N2770/24222

    摘要： The present invention relates to a method for purifying recombinant HCV single or specific oligomeric envelope proteins selected from the group consisting of E1 and/or E1/E2, characterized in that upon lysing the transformed host cells to isolate the recombinantly expressed protein a disulphide bond cleavage or reduction step is carried out with a disulphide bond cleavage agent. The present invention also relates to a composition isolated by such a method. The present invention also relates to the diagnostic and therapeutic application of these compositions. Furthermore, the invention relates to the use of HCV E1 protein and peptides for prognosing and monitoring the clinical effectiveness and/or clinical outcome of HCV treatment.

    摘要翻译： 本发明涉及一种纯化选自E1和/或E1 / E2的重组HCV单个或特异性寡聚包膜蛋白的方法，其特征在于在裂解转化的宿主细胞以分离重组表达的蛋白质时，二硫键切割 或还原步骤用二硫键切割剂进行。 本发明还涉及通过这种方法分离的组合物。 本发明还涉及这些组合物的诊断和治疗应用。 此外，本发明涉及HCV E1蛋白和肽用于预测和监测HCV治疗的临床有效性和/或临床结果的用途。

    TECHNIQUES FOR PREDICTING, DETECTING AND REDUCING ASPECIFIC PROTEIN INTERFERENCE IN ASSAYS INVOLVING IMMUNOGLOBULIN SINGLE VARIABLE DOMAINS
    10.
    发明申请
    TECHNIQUES FOR PREDICTING, DETECTING AND REDUCING ASPECIFIC PROTEIN INTERFERENCE IN ASSAYS INVOLVING IMMUNOGLOBULIN SINGLE VARIABLE DOMAINS 审中-公开
    涉及免疫球蛋白单个可变区域的测定中预测，检测和降低相关蛋白质干扰的技术

    公开(公告)号：US20140199295A1

    公开(公告)日：2014-07-17

    申请号：US14128681

    申请日：2012-06-25

    申请人： Judith Baumeister ,  Marie-Paule Lucienne Armanda Bouche ,  Carlo Boutton ,  Marie-Ange Buyse ,  Veerle Snoeck ,  Stephanie Staelens

    发明人： Judith Baumeister ,  Marie-Paule Lucienne Armanda Bouche ,  Carlo Boutton ,  Marie-Ange Buyse ,  Veerle Snoeck ,  Stephanie Staelens

    IPC分类号： C07K16/00

    CPC分类号： C07K16/28 ,  C07K16/00 ,  C07K16/4283 ,  C07K2317/22 ,  C07K2317/569 ,  C07K2317/94 ,  C07K2319/30 ,  G01N33/6854

    摘要： This invention provides, and in certain specific but non-limiting aspects relates to: assays that can be used to predict whether a given ISV will be subject to protein interference as described herein and/or give rise to an (aspecific) signal in such an assay (such as for example in an ADA immunoassay). Such predictive assays could for example be used to test whether a given ISV could have a tendency to give rise to such protein interference and/or such a signal; to select ISV's that are not or less prone to such protein interference or to giving such a signal; as an assay or test that can be used to test whether certain modification(s) to an ISV will (fully or partially) reduce its tendency to give rise to such interference or such a signal; and/or as an assay or test that can be used to guide modification or improvement of an ISV so as to reduce its tendency to give rise to such protein interference or signal; —methods for modifying and/or improving ISV's to as to remove or reduce their tendency to give rise to such protein interference or such a signal; —modifications that can be introduced into an ISV that remove or reduce its tendency to give rise to such protein interference or such a signal; ISV's that have been specifically selected (for example, using the assay(s) described herein) to have no or low(er)/reduced tendency to give rise to such protein interference or such a signal; modified and/or improved ISV's that have no or a low(er)/reduced tendency to give rise to such protein interference or such a signal.

    摘要翻译： 本发明提供并且在某些特定但非限制性方面涉及：可用于预测给定ISV是否将如本文所述受到蛋白质干扰和/或产生（特异性）信号的测定法 测定（例如在ADA免疫测定中）。 这样的预测测定可以例如用于测试给定的ISV是否可能具有引起这种蛋白质干扰和/或这种信号的倾向; 选择不是或不太容易发生蛋白质干扰的ISV或给出这样的信号; 作为可用于测试对ISV的某些修改（完全或部分）是否降低其引起这种干扰或这种信号的趋势的测定或测试; 和/或作为可用于指导ISV的修饰或改进的测定或测试，以便减少引起这种蛋白质干扰或信号的趋势; - 用于修饰和/或改善ISV的方法以消除或减少其引起这种蛋白质干扰或这种信号的倾向; - 可以引入到ISV中的改变，其去除或减少其引起这种蛋白质干扰或这种信号的趋势; 已经特别选择的ISV（例如，使用本文所述的测定）具有没有或低（呃）/降低的趋势导致这种蛋白质干扰或这种信号; 修饰和/或改进的ISV，其没有或低（呃）/降低的趋势引起这种蛋白质干扰或这种信号。