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    Displacement assay for the detection of nucleic acid oligomer hybridization events
    1.
    发明申请
    Displacement assay for the detection of nucleic acid oligomer hybridization events 审中-公开
    用于检测核酸低聚物杂交事件的位移测定

    公开(公告)号：US20060228703A1

    公开(公告)日：2006-10-12

    申请号：US10487816

    申请日：2002-08-26

    申请人： Gerhard Hartwich ,  Peter Frischmann ,  Ute Haker ,  Herbert Wieder

    发明人： Gerhard Hartwich ,  Peter Frischmann ,  Ute Haker ,  Herbert Wieder

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6825 ,  C12Q2545/107

    摘要： Described is a method for detection of nucleic acid oligomer hybridization events, the method comprising the steps: providing a modified surface, the modification consisting in the attachment of at least one type of ligate nucleic acid oligomer; providing signal nucleic acid oligomer ligands; providing a sample having nucleic acid oligomer ligands; bringing a defined quantity of the signal nucleic acid oligomer ligands into contact with the modified surface and bringing the sample and the nucleic acid oligmer ligands contained therein into contact with the modified surface; detecting the signal nucleic acid oligomer ligands; and comparing with reference values the values obtained from the detection of the signal nucleic acid oligomer ligands.

    摘要翻译： 描述了用于检测核酸寡聚体杂交事件的方法，所述方法包括以下步骤：提供修饰的表面，所述修饰组合在至少一种类型的连接核酸寡聚体的连接中; 提供信号核酸寡聚体配体; 提供具有核酸低聚物配体的样品; 使定义量的信号核酸低聚物配体与修饰表面接触并使其中含有的样品和核酸寡聚体配体与改性表面接触; 检测信号核酸低聚物配体; 并且与参考值比较，从检测信号核酸低聚物配体获得的值。

    Fluorescence-quenching used to detect nucleic acid oligomer hybrization events at high salt concentrations
    3.
    发明申请
    Fluorescence-quenching used to detect nucleic acid oligomer hybrization events at high salt concentrations 审中-公开
    荧光猝灭用于检测高盐浓度下的核酸低聚物杂化事件

    公开(公告)号：US20060188877A1

    公开(公告)日：2006-08-24

    申请号：US10529472

    申请日：2002-11-08

    申请人： Gerhard Hartwich ,  Thomas Kratzmuller ,  Herbert Wieder

    发明人： Gerhard Hartwich ,  Thomas Kratzmuller ,  Herbert Wieder

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6818 ,  C12Q2563/137 ,  C12Q2565/101 ,  C12Q2565/549 ,  C12Q2527/137

    摘要： A method is described for detecting nucleic acid oligomer hybridization events by fluorescence quenching, which comprises as a first step the provision of a modified surface. The modification of the surface consists in the binding of at least one type of modified nucleic acid oligomers 201, wherein said nucleic acid oligomers 201 are modified by at least one type of fluorophore 102 bound to it. The further steps of the inventive method are: providing a sample that includes nucleic acid oligomers, contacting said sample with the modified surface, adjusting a defined salt concentration of greater than 0.5 mol/l in the solution surrounding the modified nucleic acid oligomers, detecting the fluorescence of the fluorophore and comparing the detected fluorescence intensity with reference values.

    摘要翻译： 描述了通过荧光猝灭检测核酸低聚物杂交事件的方法，其包括提供改性表面的第一步骤。 表面的修饰在于至少一种类型的修饰的核酸低聚物201的结合，其中所述核酸寡聚物201被与其结合的至少一种类型的荧光团102修饰。 本发明方法的进一步步骤是：提供包含核酸低聚物的样品，使所述样品与修饰表面接触，调节在修饰的核酸寡聚物周围的溶液中大于0.5mol / l的限定的盐浓度，检测 荧光团的荧光，并将检测到的荧光强度与参考值进行比较。

    Electrical substrate for use as a carrier of biomolecules
    4.
    发明申请
    Electrical substrate for use as a carrier of biomolecules 审中-公开
    用作生物分子载体的电气底物

    公开(公告)号：US20060275925A1

    公开(公告)日：2006-12-07

    申请号：US10539463

    申请日：2003-12-23

    申请人： Gerhard Hartwich ,  Harald Lossau ,  Herbert Wieder ,  Norbert Persike ,  Christian Musewald

    发明人： Gerhard Hartwich ,  Harald Lossau ,  Herbert Wieder ,  Norbert Persike ,  Christian Musewald

    IPC分类号： H01L21/00

    CPC分类号： G01N33/5438 ,  G01N27/3276

    摘要： An electrical substrate for use as a carrier of biomolecules in a method for electrochemical detection in an electrolyte solution exhibits an insulating support plate (12) bearing a conductive pattern (20; 20A-20C, 28) having conductor paths (20; 20A-20C) and connecting contact surfaces, and, disposed on the conductor paths (20; 20A-20C), test sites (24) for the application of biomolecules (26), the conductor paths (20; 20A-20C) exhibiting a metal core (14) made of a highly conductive base metal and an external gold layer (18), and the conductor paths (20; 20A-20C) being provided with a diffusion barrier layer (16) that prevents direct contact of the electrolyte solution with the metal core (14) during the electrochemical detection method.

    摘要翻译： 在用于电解质溶液中的电化学检测方法中用作生物分子载体的电气基质表现出具有导电图案（20; 20A-20C，28）的绝缘支撑板（20），其具有导体路径（20; 20 所述导体路径（20; 20A-20C）和连接接触表面，并且设置在所述导体路径（20,20A-20C）上，用于施加生物分子（26）的测试位置（24） 20），具有由高导电性基体金属和外部金层（18）制成的金属芯（14），并且所述导体路径（20; 20A-20C）设置有扩散阻挡层（16），所述扩散阻挡层 在电化学检测方法期间防止电解质溶液与金属芯（14）的直接接触。

    METHODS FOR ELECTROCHEMICAL ANALYSIS USING MATRIX COMPOSITIONS WITH ALKYLPHENAZINE QUATERNARY SALT AND A NITROSALINE
    5.
    发明申请
    METHODS FOR ELECTROCHEMICAL ANALYSIS USING MATRIX COMPOSITIONS WITH ALKYLPHENAZINE QUATERNARY SALT AND A NITROSALINE 有权
    电化学分析方法使用基质组合物与烷基盐酸季铵盐和硝酸盐

    公开(公告)号：US20110290670A1

    公开(公告)日：2011-12-01

    申请号：US13208420

    申请日：2011-08-12

    申请人： Christopher D. Wilsey ,  Mitali Ghoshal ,  Herbert Wieder

    发明人： Christopher D. Wilsey ,  Mitali Ghoshal ,  Herbert Wieder

    IPC分类号： G01N27/28 ,  G01N33/66

    CPC分类号： C12Q1/006 ,  C12Q1/004 ,  G01N2333/904

    摘要： A chemistry matrix for use in determining the concentration of an analyte in a biological fluid includes a glucose dehydrogenase, nicotinamide adenine dinucleotide, an alkylphenazine quaternary salt, and/or a nitrosoaniline. The chemistry matrix is used with an electrochemical biosensor to determine the concentration of an analyte after a reaction occurs within the biosensor, at which time an analysis is completed to determine the concentration. A method of determining the concentration of an analyte using the chemistry matrix of glucose dehydrogenase, nicotinamide adenine dinucleotide, an alkylphenazine quaternary salt, and/or a nitrosoaniline is another aspect that is described. The method also further features test times of five seconds or less. Methods utilizing the new chemistry matrix can readily determine an analyte such as blood glucose at concentrations of from about 20-600 mg/dL at a pH of from about 6.5 to about 8.5.

    摘要翻译： 用于测定生物流体中分析物浓度的化学基质包括葡萄糖脱氢酶，烟酰胺腺嘌呤二核苷酸，烷基吩嗪季盐和/或亚硝基苯胺。 化学基质与电化学生物传感器一起使用以确定在生物传感器内发生反应后分析物的浓度，此时完成分析以确定浓度。 使用葡萄糖脱氢酶，烟酰胺腺嘌呤二核苷酸，烷基吩嗪季盐和/或亚硝基苯胺的化学基质测定分析物的浓度的方法是描述的另一方面。 该方法还进一步具有5秒或更短的测​​试时间。 利用新化学基质的方法可以容易地确定分析物，例如浓度为约20-600mg / dL的分析物，pH为约6.5至约8.5。

    Electrical connection system for electrochemical analysis system
    6.
    发明授权
    Electrical connection system for electrochemical analysis system 失效
    电化学分析系统电气连接系统

    公开(公告)号：US07754152B2

    公开(公告)日：2010-07-13

    申请号：US11295187

    申请日：2005-12-06

    申请人： Stefan Riebel ,  Bruno Robert Thoes ,  Manfred Augstein ,  Herbert Wieder ,  Gregor Bainczyk

    发明人： Stefan Riebel ,  Bruno Robert Thoes ,  Manfred Augstein ,  Herbert Wieder ,  Gregor Bainczyk

    IPC分类号： G01N27/00

    CPC分类号： G01N33/4875 ,  Y10T29/49002

    摘要： The invention relates to an electrical connection system for an analysis system and a method for analysis of a liquid sample on an analytical test element using the described analysis system. The analysis system comprises an evaluation appliance for evaluation of electrical signals, a test element holder for holding and positioning of an analytical test element in a measurement position, and at least one electrical contact element configured to make electrical contact with at least one electrical contact surface of an analytical test element, and to produce an electrical connection between the contact surface and the evaluation appliance. The at least one contact element is arranged to move to contact the contact surface of a test element held either in a fixed test element holder or a moveable test element holder, such as a slide. Furthermore, the analysis system has a means for moving the electrical contact element to the electrical contact surface of an analytical test element when the test element holder is in the measurement position.

    摘要翻译： 本发明涉及用于分析系统的电连接系统和使用所述分析系统分析测试元件上的液体样品的方法。 分析系统包括用于评估电信号的评估装置，用于在测量位置保持和定位分析测试元件的测试元件保持器，以及配置成与至少一个电接触表面进行电接触的至少一个电接触元件 的分析测试元件，并且在接触表面和评估设备之间产生电连接。 所述至少一个接触元件布置成移动以接触保持在固定测试元件保持器或可移动测试元件保持器（例如滑块）中的测试元件的接触表面。 此外，分析系统具有当测试元件保持器处于测量位置时将电接触元件移动到分析测试元件的电接触表面的装置。

    BIOSENSOR WITH IMPROVED ANALYTE SPECIFICITY
    7.
    发明申请
    BIOSENSOR WITH IMPROVED ANALYTE SPECIFICITY 有权
    生物传感器具有改进的分析特异性

    公开(公告)号：US20090246808A1

    公开(公告)日：2009-10-01

    申请号：US12056473

    申请日：2008-03-27

    申请人： Christopher D. Wilsey ,  Mitali Ghoshal ,  Herbert Wieder

    发明人： Christopher D. Wilsey ,  Mitali Ghoshal ,  Herbert Wieder

    IPC分类号： C12Q1/54

    CPC分类号： C12Q1/006 ,  C12Q1/004 ,  G01N2333/904

    摘要： A chemistry matrix for use in determining the concentration of an analyte in a biological fluid includes a glucose dehydrogenase, nicotinamide adenine dinucleotide, an alkylphenazine quaternary salt, and/or a nitrosoaniline. The chemistry matrix is used with an electrochemical biosensor to determine the concentration of an analyte after a reaction occurs within the biosensor, at which time an analysis is completed to determine the concentration. A method of determining the concentration of an analyte using the chemistry matrix of glucose dehydrogenase, nicotinamide adenine dinucleotide, an alkylphenazine quaternary salt, and/or a nitrosoaniline is another aspect that is described. The method also further features test times of five seconds or less. Methods utilizing the new chemistry matrix can readily determine an analyte such as blood glucose at concentrations of from about 20-600 mg/dL at a pH of from about 6.5 to about 8.5.

    摘要翻译： 用于测定生物流体中分析物浓度的化学基质包括葡萄糖脱氢酶，烟酰胺腺嘌呤二核苷酸，烷基吩嗪季盐和/或亚硝基苯胺。 化学基质与电化学生物传感器一起使用以确定在生物传感器内发生反应后分析物的浓度，此时完成分析以确定浓度。 使用葡萄糖脱氢酶，烟酰胺腺嘌呤二核苷酸，烷基吩嗪季盐和/或亚硝基苯胺的化学基质测定分析物的浓度的方法是描述的另一方面。 该方法还进一步具有5秒或更短的测​​试时间。 利用新化学基质的方法可以容易地确定分析物，例如浓度为约20-600mg / dL的分析物，pH为约6.5至约8.5。