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1.发明申请Materials and kits for use in hot-start PCR, and methods of amplifying nucleic acids in a polymerase chain reaction 审中-公开用于热启动PCR的材料和试剂盒，以及在聚合酶链式反应中扩增核酸的方法公开(公告)号：US20060263811A1
公开(公告)日：2006-11-23
申请号：US11416784
申请日：2006-05-03
申请人： GeunSook Jeon , Charles Vann , Achim Karger , Aldrich Lau , Shaheer Khan
发明人： GeunSook Jeon , Charles Vann , Achim Karger , Aldrich Lau , Shaheer Khan
CPC分类号： C12Q1/6848 , C12Q1/6806 , C12Q1/686 , C12Q2549/101 , C12Q2527/125 , C12Q2547/107
摘要： Materials for sequestering reagents in hot-start PCR, kits containing such materials, and methods for the use of such materials in amplifying nucleic acids are described.
摘要翻译： 描述了在热启动PCR中螯合试剂的材料，含有这些材料的试剂盒，以及在扩增核酸中使用这些材料的方法。
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2.发明申请Universal bases for nucleic acid analyses, methods for using universal bases, and kits comprising universal bases 失效用于核酸分析的普遍基础，使用通用碱的方法和包含通用碱基的试剂盒公开(公告)号：US20080274462A1
公开(公告)日：2008-11-06
申请号：US11796555
申请日：2007-04-27
申请人： Geunsook Jeon , Shaheer Khan
发明人： Geunsook Jeon , Shaheer Khan
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , Y10S435/975 , C12Q2535/125 , C12Q2525/117 , C12Q2525/101 , C12Q2535/101
摘要： Compounds, methods and kits for making and analyzing primer extension products incorporating one or more universal bases are described, including methods and kits for nucleic acid sequencing and microsatellite analysis.
摘要翻译： 描述了用于制备和分析引入一个或多个通用碱基的引物延伸产物的化合物，方法和试剂盒，包括用于核酸测序和微卫星分析的方法和试剂盒。
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3.发明授权Universal bases for nucleic acid analyses, methods for using universal bases, and kits comprising universal bases 失效用于核酸分析的普遍基础，使用通用碱的方法和包含通用碱基的试剂盒公开(公告)号：US07214783B2
公开(公告)日：2007-05-08
申请号：US10877511
申请日：2004-06-25
申请人： Geunsook Jeon , Shaheer Khan
发明人： Geunsook Jeon , Shaheer Khan
CPC分类号： C12Q1/6869 , Y10S435/975 , C12Q2535/125 , C12Q2525/117 , C12Q2525/101 , C12Q2535/101
摘要： Compounds, methods and kits for making and analyzing primer extension products incorporating one or more universal bases are described, including methods and kits for nucleic acid sequencing and microsatellite analysis.
摘要翻译： 描述了用于制备和分析引入一个或多个通用碱基的引物延伸产物的化合物，方法和试剂盒，包括用于核酸测序和微卫星分析的方法和试剂盒。
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4.发明申请Universal bases for nucleic acid analyses, methods for using universal bases, and kits comprising universal bases 失效用于核酸分析的普遍基础，使用通用碱的方法和包含通用碱基的试剂盒公开(公告)号：US20050037396A1
公开(公告)日：2005-02-17
申请号：US10877511
申请日：2004-06-25
申请人： Geunsook Jeon , Shaheer Khan
发明人： Geunsook Jeon , Shaheer Khan
IPC分类号： C12N15/09 , C07D471/02 , C07H19/22 , C07H21/04 , C12Q1/68
CPC分类号： C12Q1/6869 , Y10S435/975 , C12Q2535/125 , C12Q2525/117 , C12Q2525/101 , C12Q2535/101
摘要： Compounds, methods and kits for making and analyzing primer extension products incorporating one or more universal bases are described, including methods and kits for nucleic acid sequencing and microsatellite analysis.
摘要翻译： 描述了用于制备和分析引入一个或多个通用碱基的引物延伸产物的化合物，方法和试剂盒，包括用于核酸测序和微卫星分析的方法和试剂盒。
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公开(公告)号：US20050250119A1
公开(公告)日：2005-11-10
申请号：US10977341
申请日：2004-10-28
申请人： Meng Taing , Shaheer Khan , Steven Menchen , Barnett Rosenblum
发明人： Meng Taing , Shaheer Khan , Steven Menchen , Barnett Rosenblum
IPC分类号： G01N21/64 , C07B61/00 , C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C12N15/09 , C12Q1/68 , G01N21/77 , G01N21/78 , G01N27/447 , G01N33/483 , G01N33/50 , G01N33/53 , G01N33/566 , G01N33/58 , C07H21/04
摘要： Provided are nucleotide-dye conjugates and related compounds in which a dye is linked to a nucleobase directly or indirectly by an anionic linker. The anionic character of the linker is provided by one or more anionic moieties which are present in the linker, such as phosphate, phosphonate, sulfonate, and carboxylate groups. When the dye is a provided as a donor/acceptor dye pair, the anionic linker can be located between the donor and the acceptor, or between the nucleobase and either the donor or acceptor, or both. In one embodiment, conjugates of the invention provide enhanced electrophoretic mobility characteristics to sequencing fragments, e.g., for dideoxy sequencing using labeled terminators.
摘要翻译： 提供的是核苷酸 - 染料缀合物和相关化合物，其中染料通过阴离子连接体直接或间接连接到核碱基。 连接体的阴离子特征由连接物中存在的一个或多个阴离子部分提供，例如磷酸酯，膦酸酯，磺酸酯和羧酸酯基团。 当染料作为供体/受体染料对提供时，阴离子连接体可以位于供体和受体之间，或位于核碱基与供体或受体之间或两者之间。 在一个实施方案中，本发明的缀合物为测序片段提供增强的电泳迁移率特性，例如使用标记的终止子进行双脱氧测序。
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公开(公告)号：US08835625B2
公开(公告)日：2014-09-16
申请号：US13470181
申请日：2012-05-11
申请人： Barnett Rosenblum , Geun-sook Jeon , Shaheer Khan
发明人： Barnett Rosenblum , Geun-sook Jeon , Shaheer Khan
摘要： Disclosed, among other things, are compounds having the structure wherein X comprises a bond or a linker, LABEL comprises at least one detectable label, W1 taken alone is —H or —OH, W2 is —OH or a non-extendable moiety, W3 when taken alone is —H or when taken together with W1 is —CH2—O—, and W4 is OH, monophosphate, diphosphate, or triphosphate. Also disclosed are labeled polynucleotide compounds and methods of use thereof.
摘要翻译： 公开了具有以下结构的化合物，其中X包含键或连接基，LABEL包含至少一个可检测标记，单独使用的W1为-H或-OH，W2为-OH或不可延伸部分，W3 当单独使用-H或当与W1一起为-CH 2 -O-，W 4为OH，单磷酸酯，二磷酸酯或三磷酸酯时。 还公开了标记的多核苷酸化合物及其使用方法。
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公开(公告)号：US20050214843A1
公开(公告)日：2005-09-29
申请号：US11104130
申请日：2005-04-12
申请人： Barnett Rosenblum , Geun-sook Jeon , Shaheer Khan
发明人： Barnett Rosenblum , Geun-sook Jeon , Shaheer Khan
摘要： The present teachings relate to nucleobase, nucleoside and nucleotide compounds, methods of synthesis, and uses thereof. The present teachings provide compounds, such as nucleobase, nucleoside and/or nucleotide compounds including a propargyl linker, and methods for making or using such compounds.
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公开(公告)号：US20050170388A1
公开(公告)日：2005-08-04
申请号：US11015319
申请日：2004-12-17
申请人： Barnett Rosenblum , Geun-sook Jeon , Shaheer Khan
发明人： Barnett Rosenblum , Geun-sook Jeon , Shaheer Khan
摘要： The present teachings relate to nucleobase, nucleoside and nucleotide compounds, methods of synthesis, and uses thereof. The present teachings provide compounds, such as nucleobase, nucleoside and/or nucleotide compounds including a propargyl linker, and methods for making or using such compounds.
摘要翻译： 本教导涉及核碱基，核苷和核苷酸化合物，合成方法及其用途。 本发明提供化合物，例如核碱基，包括炔丙基接头的核苷和/或核苷酸化合物，以及制备或使用这些化合物的方法。
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公开(公告)号：US20070293662A1
公开(公告)日：2007-12-20
申请号：US11797586
申请日：2007-05-04
申请人： Peter Fisher , Paolo Vatta , Shaheer Khan
发明人： Peter Fisher , Paolo Vatta , Shaheer Khan
IPC分类号： C07H21/04
摘要： The invention provides novel dye-labeled ribonucleotide analogs and methods for synthesizing those analogs. The compounds of the invention are especially useful for DNA sequencing by the polymerase chain reaction.
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公开(公告)号：US20060286561A1
公开(公告)日：2006-12-21
申请号：US11277481
申请日：2006-03-24
申请人： Shaheer Khan , Steven Menchen , Barnett Rosenblum
发明人： Shaheer Khan , Steven Menchen , Barnett Rosenblum
摘要： Propargylethoxyamino nucleosides are disclosed having the structure wherein R1 and R2 are —H lower alkyl, or label; B is a 7-deazapurine, purine, or pyrimidine nucleoside base; W1 is —H or —H; W2 is —H or a moiety which renders the nucleoside incapable of forming a phosphodiester bond at the 3′-position; and W3 is —PO4, —P2O7, —P3O10, phosphate analog, or —OH. Additionaly, a primer extension method is provided employing the above propargylethoxyamino nucleosides.
摘要翻译： 公开了具有其中R 1和R 2为-H低级烷基或标记的结构的丙炔乙氧基氨基核苷; B是7-脱氮嘌呤，嘌呤或嘧啶核苷碱基; W 1是-H或-H; W 2是-H或使核苷不能在3'-位形成磷酸二酯键的部分; 和W 3是-PO 4，-P 2 O 7，-P 3， 或磷酸酯类似物或-OH。 另外，使用上述炔丙氧基氨基核苷提供引物延伸方法。
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