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公开(公告)号：US20090011959A1
公开(公告)日：2009-01-08
申请号：US12154901
申请日：2008-05-27
IPC分类号： C40B50/14
CPC分类号： C12Q1/686 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/5085 , B01L7/52 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , C12N15/1093 , C12Q1/6806 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q1/6867 , C12Q1/6869 , C12Q1/6874 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N2021/6484 , G01R33/1269 , Y02P20/582 , Y10T436/143333 , C12Q2565/119 , C12Q2531/113 , C12Q2565/537 , C12Q2547/107 , C12Q2547/101 , C12Q2537/143 , C12Q2527/125 , C12Q2525/186 , C12Q2565/301 , C12Q2563/149 , C12Q2531/125 , C12Q2537/149 , C12Q2535/119
摘要： This invention relates to methods of generating single stranded DNA libraries for use in amplification and sequencing reactions. In various aspects, the disclosed methods include: fragmenting DNA; polishing the fragments' ends; ligating the fragments to universal adaptors; performing strand displacement and extension of the nicked fragments; purifying the double-stranded ligation products; capturing the double-stranded ligation products onto a solid support; and isolating single stranded DNA library fragments, and binding these fragments to another solid support.
摘要翻译： 本发明涉及产生用于扩增和测序反应的单链DNA文库的方法。 在各方面，所公开的方法包括：片段化DNA; 抛光片段的末端; 将碎片连接到通用适配器; 进行缝合位移和切口碎片的延伸; 纯化双链结合产物; 将双链结合产物捕获到固体支持物上; 并分离单链DNA文库片段，并将这些片段结合到另一种固体支持物上。
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公开(公告)号：US07323305B2
公开(公告)日：2008-01-29
申请号：US10767779
申请日：2004-09-22
CPC分类号： C12Q1/686 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/5085 , B01L7/52 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , C12N15/1093 , C12Q1/6806 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q1/6867 , C12Q1/6869 , C12Q1/6874 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N2021/6484 , G01R33/1269 , Y02P20/582 , Y10T436/143333 , C12Q2565/119 , C12Q2531/113 , C12Q2565/537 , C12Q2547/107 , C12Q2547/101 , C12Q2537/143 , C12Q2527/125 , C12Q2525/186 , C12Q2565/301 , C12Q2563/149 , C12Q2531/125 , C12Q2537/149 , C12Q2535/119
摘要： An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step.
摘要翻译： 本文提供了用于进行快速DNA测序的装置和方法，例如基因组测序。 该方法包括以下步骤：制备用于基因组测序的样品DNA，以代表性方式扩增所制备的DNA，并仅在一个引物杂交步骤对经扩增的DNA进行多次测序反应。
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公开(公告)号：US08748102B2
公开(公告)日：2014-06-10
申请号：US13033240
申请日：2011-02-23
申请人： Jan Berka , Yi-Ju Chen , John H. Leamon , Steve Lefkowitz , Kenton L. Lohman , Vinod B. Makhijani , Jonathan M. Rothberg , Gary J. Sarkis , Maithreyan Srinivasan , Michael P. Weiner
发明人： Jan Berka , Yi-Ju Chen , John H. Leamon , Steve Lefkowitz , Kenton L. Lohman , Vinod B. Makhijani , Jonathan M. Rothberg , Gary J. Sarkis , Maithreyan Srinivasan , Michael P. Weiner
CPC分类号： C12Q1/686 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/5085 , B01L7/52 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , C12N15/1093 , C12Q1/6806 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q1/6867 , C12Q1/6869 , C12Q1/6874 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N2021/6484 , G01R33/1269 , Y02P20/582 , Y10T436/143333 , C12Q2565/119 , C12Q2531/113 , C12Q2565/537 , C12Q2547/107 , C12Q2547/101 , C12Q2537/143 , C12Q2527/125 , C12Q2525/186 , C12Q2565/301 , C12Q2563/149 , C12Q2531/125 , C12Q2537/149 , C12Q2535/119
摘要： Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.
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公开(公告)号：US07244567B2
公开(公告)日：2007-07-17
申请号：US10768729
申请日：2004-01-28
申请人： Yi-Ju Chen , John H. Leamon , Kenton L. Lohman , Michael T. Ronan , Jonathan M. Rothberg , Maithreyan Srinivasan , Michael P. Weiner
发明人： Yi-Ju Chen , John H. Leamon , Kenton L. Lohman , Michael T. Ronan , Jonathan M. Rothberg , Maithreyan Srinivasan , Michael P. Weiner
CPC分类号： C12Q1/686 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/5085 , B01L7/52 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , C12N15/1093 , C12Q1/6806 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q1/6867 , C12Q1/6869 , C12Q1/6874 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N2021/6484 , G01R33/1269 , Y02P20/582 , Y10T436/143333 , C12Q2565/119 , C12Q2531/113 , C12Q2565/537 , C12Q2547/107 , C12Q2547/101 , C12Q2537/143 , C12Q2527/125 , C12Q2525/186 , C12Q2565/301 , C12Q2563/149 , C12Q2531/125 , C12Q2537/149 , C12Q2535/119
摘要： This invention relates to methods of sequencing DNA. More specifically, this invention relates to methods of sequencing both the sense and antisense strands of DNA through the use of blocked and unblocked sequencing primers. In brief, these methods include the steps of annealing an unblocked primer to a first strand of nucleic acid; annealing a second blocked primer to a second strand of nucleic acid; elongating the nucleic acid along the first strand with a polymerase; terminating the first sequencing primer; deblocking the second primer; and elongating the nucleic acid along the second strand.
摘要翻译： 本发明涉及DNA测序方法。 更具体地，本发明涉及通过使用封闭和未封闭的测序引物对DNA的有义链和反义链进行测序的方法。 简而言之，这些方法包括将未封闭引物退火至第一条核酸的步骤; 将第二封闭引物退火至核酸的第二链; 用聚合酶沿着第一链延长核酸; 终止第一个测序引物; 解封第二底漆; 并沿着第二链延长核酸。
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公开(公告)号：US08765380B2
公开(公告)日：2014-07-01
申请号：US13618334
申请日：2012-09-14
申请人： Jan Berka , Yi-Ju Chen , John H. Leamon , Steve Lefkowitz , Kenton L. Lohman , Vinod B. Makhijani , Jonathan M. Rothberg , Gary J. Sarkis , Maithreyn Srinivasan , Michael P. Weiner
发明人： Jan Berka , Yi-Ju Chen , John H. Leamon , Steve Lefkowitz , Kenton L. Lohman , Vinod B. Makhijani , Jonathan M. Rothberg , Gary J. Sarkis , Maithreyn Srinivasan , Michael P. Weiner
CPC分类号： C12Q1/686 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/5085 , B01L7/52 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , C12N15/1093 , C12Q1/6806 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q1/6867 , C12Q1/6869 , C12Q1/6874 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N2021/6484 , G01R33/1269 , Y02P20/582 , Y10T436/143333 , C12Q2565/119 , C12Q2531/113 , C12Q2565/537 , C12Q2547/107 , C12Q2547/101 , C12Q2537/143 , C12Q2527/125 , C12Q2525/186 , C12Q2565/301 , C12Q2563/149 , C12Q2531/125 , C12Q2537/149 , C12Q2535/119
摘要： Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.
摘要翻译： 公开了用于核酸扩增的方法，其中将核酸模板，珠粒和扩增反应溶液乳化，并扩增核酸模板以提供附着于珠粒上的核酸模板的克隆拷贝。 还公开了用于执行本发明的方法的套件和装置。
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公开(公告)号：US08158359B2
公开(公告)日：2012-04-17
申请号：US12803362
申请日：2010-06-24
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/5085 , B01L7/52 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , C12N15/1093 , C12Q1/6806 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q1/6867 , C12Q1/6869 , C12Q1/6874 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N2021/6484 , G01R33/1269 , Y02P20/582 , Y10T436/143333 , C12Q2565/119 , C12Q2531/113 , C12Q2565/537 , C12Q2547/107 , C12Q2547/101 , C12Q2537/143 , C12Q2527/125 , C12Q2525/186 , C12Q2565/301 , C12Q2563/149 , C12Q2531/125 , C12Q2537/149 , C12Q2535/119
摘要： An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step.
摘要翻译： 本文提供了用于进行快速DNA测序的装置和方法，例如基因组测序。 该方法包括以下步骤：制备用于基因组测序的样品DNA，以代表性方式扩增所制备的DNA，并仅在一个引物杂交步骤对经扩增的DNA进行多次测序反应。
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公开(公告)号：US07842457B2
公开(公告)日：2010-11-30
申请号：US10767899
申请日：2004-01-28
申请人： Jan Berka , Yi-Ju Chen , John H. Leamon , Steve Lefkowitz , Kenton L. Lohman , Vinod B. Makhijani , Jonathan M. Rothberg , Gary J. Sarkis , Maithreyan Srinivasan , Michael P. Weiner
发明人： Jan Berka , Yi-Ju Chen , John H. Leamon , Steve Lefkowitz , Kenton L. Lohman , Vinod B. Makhijani , Jonathan M. Rothberg , Gary J. Sarkis , Maithreyan Srinivasan , Michael P. Weiner
CPC分类号： C12Q1/686 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/5085 , B01L7/52 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , C12N15/1093 , C12Q1/6806 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q1/6867 , C12Q1/6869 , C12Q1/6874 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N2021/6484 , G01R33/1269 , Y02P20/582 , Y10T436/143333 , C12Q2565/119 , C12Q2531/113 , C12Q2565/537 , C12Q2547/107 , C12Q2547/101 , C12Q2537/143 , C12Q2527/125 , C12Q2525/186 , C12Q2565/301 , C12Q2563/149 , C12Q2531/125 , C12Q2537/149 , C12Q2535/119
摘要： Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.
摘要翻译： 公开了用于核酸扩增的方法，其中将核酸模板，珠粒和扩增反应溶液乳化，并扩增核酸模板以提供附着于珠粒上的核酸模板的克隆拷贝。 还公开了用于执行本发明的方法的套件和装置。
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公开(公告)号：US20130078638A1
公开(公告)日：2013-03-28
申请号：US13618334
申请日：2012-09-14
申请人： Jan Berka , Yi-Ju Chen , John H. Leamon , Steve Lefkowitz , Kenton L. Lohman , Vinod B. Makhijani , Jonathan M. Rothberg , Gary J. Sarkis , Maithreyan Srinivasan , Michael P. Weiner
发明人： Jan Berka , Yi-Ju Chen , John H. Leamon , Steve Lefkowitz , Kenton L. Lohman , Vinod B. Makhijani , Jonathan M. Rothberg , Gary J. Sarkis , Maithreyan Srinivasan , Michael P. Weiner
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/5085 , B01L7/52 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , C12N15/1093 , C12Q1/6806 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q1/6867 , C12Q1/6869 , C12Q1/6874 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N2021/6484 , G01R33/1269 , Y02P20/582 , Y10T436/143333 , C12Q2565/119 , C12Q2531/113 , C12Q2565/537 , C12Q2547/107 , C12Q2547/101 , C12Q2537/143 , C12Q2527/125 , C12Q2525/186 , C12Q2565/301 , C12Q2563/149 , C12Q2531/125 , C12Q2537/149 , C12Q2535/119
摘要： Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the heads. Also disclosed are kits and apparatuses for performing the methods of the invention.
摘要翻译： 公开了核酸扩增的方法，其中将核酸模板，珠粒和扩增反应溶液乳化并扩增核酸模板以提供附着于头部的核酸模板的克隆拷贝。 还公开了用于执行本发明的方法的套件和装置。
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公开(公告)号：US08012690B2
公开(公告)日：2011-09-06
申请号：US11982095
申请日：2007-10-31
申请人： Jan Berka , Yi-Ju Chen , John H. Leamon , Steve Lefkowitz , Kenton L. Lohman , Vinod B. Makhijani , Jonathan M. Rothberg , Gary J. Sarkis , Maithreyan Srinivasan , Michael P. Weiner
发明人： Jan Berka , Yi-Ju Chen , John H. Leamon , Steve Lefkowitz , Kenton L. Lohman , Vinod B. Makhijani , Jonathan M. Rothberg , Gary J. Sarkis , Maithreyan Srinivasan , Michael P. Weiner
CPC分类号： C12Q1/686 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/5085 , B01L7/52 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , C12N15/1093 , C12Q1/6806 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q1/6867 , C12Q1/6869 , C12Q1/6874 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N2021/6484 , G01R33/1269 , Y02P20/582 , Y10T436/143333 , C12Q2565/119 , C12Q2531/113 , C12Q2565/537 , C12Q2547/107 , C12Q2547/101 , C12Q2537/143 , C12Q2527/125 , C12Q2525/186 , C12Q2565/301 , C12Q2563/149 , C12Q2531/125 , C12Q2537/149 , C12Q2535/119
摘要： Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.
摘要翻译： 公开了用于核酸扩增的方法，其中将核酸模板，珠粒和扩增反应溶液乳化，并扩增核酸模板以提供附着于珠粒上的核酸模板的克隆拷贝。 还公开了用于执行本发明的方法的套件和装置。
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公开(公告)号：US20110009275A1
公开(公告)日：2011-01-13
申请号：US12803362
申请日：2010-06-24
CPC分类号： C12Q1/686 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/5085 , B01L7/52 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , C12N15/1093 , C12Q1/6806 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q1/6867 , C12Q1/6869 , C12Q1/6874 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N2021/6484 , G01R33/1269 , Y02P20/582 , Y10T436/143333 , C12Q2565/119 , C12Q2531/113 , C12Q2565/537 , C12Q2547/107 , C12Q2547/101 , C12Q2537/143 , C12Q2527/125 , C12Q2525/186 , C12Q2565/301 , C12Q2563/149 , C12Q2531/125 , C12Q2537/149 , C12Q2535/119
摘要： An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step.
摘要翻译： 本文提供了用于进行快速DNA测序的装置和方法，例如基因组测序。 该方法包括以下步骤：制备用于基因组测序的样品DNA，以代表性方式扩增所制备的DNA，并仅在一个引物杂交步骤对经扩增的DNA进行多次测序反应。
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