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    Methods for detection and analysis of polynucleotide-binding protein interactions
    2.
    发明授权
    Methods for detection and analysis of polynucleotide-binding protein interactions 有权
    检测和分析多核苷酸结合蛋白相互作用的方法

    公开(公告)号：US08993335B2

    公开(公告)日：2015-03-31

    申请号：US10779412

    申请日：2004-02-13

    申请人： Guillermo C. Bazan ,  Shu Wang ,  Bin Liu

    发明人： Guillermo C. Bazan ,  Shu Wang ,  Bin Liu

    IPC分类号： G01N33/00 ,  C12Q1/68 ,  C07K1/00 ,  C07H21/02

    CPC分类号： C12Q1/6818 ,  Y10T436/143333 ,  C12Q2565/101

    摘要： Methods, compositions and articles of manufacture for assaying a sample for a target polynucleotide are provided. A sample suspected of containing the target polynucleotide is contacted with a polycationic multichromophore and a sensor PBP that can bind to the target polynucleotide. The sensor PBP comprises a signaling chromophore to absorb energy from the excited multichromophore and emit light in the presence of the target polynucleotide. The methods can be used in multiplex form. Kits comprising reagents for performing such methods are also provided.

    摘要翻译： 提供了用于测定靶多核苷酸样品的方法，组合物和制品。 将怀疑含有靶多核苷酸的样品与聚阳离子多重色素和可与靶多核苷酸结合的传感器PBP接触。 传感器PBP包括信号发色团，以吸收来自激发的多色素的能量并在靶多核苷酸的存在下发光。 该方法可以多路复用形式使用。 还提供了包含用于执行这些方法的试剂的试剂盒。

    Methods and articles for strand-specific polynucleotide detection with conjugated polymers
    3.
    发明授权
    Methods and articles for strand-specific polynucleotide detection with conjugated polymers 有权
    用共轭聚合物进行链特异性多核苷酸检测的方法和文章

    公开(公告)号：US08669055B2

    公开(公告)日：2014-03-11

    申请号：US12903176

    申请日：2010-10-12

    申请人： Guillermo C. Bazan ,  Bin Liu

    发明人： Guillermo C. Bazan ,  Bin Liu

    IPC分类号： C12Q1/68 ,  C12M1/34 ,  C07H19/00 ,  C07H21/04 ,  C07H21/02 ,  G01N21/64

    CPC分类号： C12Q1/6818 ,  C12Q2563/119 ,  C12Q2565/101 ,  C12Q2565/519 ,  C12Q2565/607 ,  C12Q2523/313

    摘要： The invention further relates to conjugated polymers, and methods, articles and compositions employing them as described herein. In some aspects, the invention relates to methods, articles and compositions for the detection and analysis of biomolecules in a sample. Provided assays include those determining the presence of a target biomolecule in a sample or its relative amount, or the assays may be quantitative or semi-quantitative. The methods can be performed on a substrate. The methods can be performed in an array format on a substrate, which can be a sensor. In some embodiments, detection assays are provided employing sensor biomolecules that do not comprise a fluorophore that can exchange energy with the cationic multichromophore. In some aspects biological assays are provided in which energy is transferred between one or more of the multichromophore, a label on the target biomolecule, a label on the sensor biomolecule, and/or a fluorescent dye specific for a polynucleotide, in all permutations. The multichromophore may interact at least in part electrostatically with the sensor and/or the target, and an increase in energy transfer with the polymer may occur upon binding of the sensor and the target. Other variations of the inventions are described further herein.

    摘要翻译： 本发明还涉及共轭聚合物，以及如本文所述使用它们的方法，制品和组合物。 在一些方面，本发明涉及用于检测和分析样品中生物分子的方法，制品和组合物。 提供的测定包括确定样品中靶生物分子的存在或其相对量的那些，或者测定可以是定量或半定量的。 该方法可以在衬底上进行。 这些方法可以以阵列格式在基底上进行，其可以是传感器。 在一些实施方案中，使用不包含能够与阳离子多重色素交换能量的荧光团的传感器生物分子来提供检测测定。 在一些方面，提供生物测定，其中在所有置换中，能量在一个或多个多色素，目标生物分子上的标记，传感器生物分子上的标记和/或多核苷酸特异的荧光染料之间转移。 多重色淀可以至少部分地与传感器和/或靶相互作用，并且当传感器和目标物结合时，聚合物的能量传递的增加可能发生。 本文进一步描述了本发明的其它变型。

    Conjugated polymers for use in homogeneous and solid state assays
    5.
    发明授权
    Conjugated polymers for use in homogeneous and solid state assays 有权
    用于均相和固态测定的共轭聚合物

    公开(公告)号：US08338532B2

    公开(公告)日：2012-12-25

    申请号：US13038378

    申请日：2011-03-01

    申请人： Guillermo C. Bazan ,  Bin Liu

    发明人： Guillermo C. Bazan ,  Bin Liu

    IPC分类号： C08G63/91 ,  C08G63/00 ,  C12Q1/68

    CPC分类号： C08G61/02 ,  C08G61/122 ,  C08G61/123 ,  C12Q1/6818 ,  C12Q1/6837 ,  G01N21/6428 ,  G01N21/6452 ,  G01N2021/6419 ,  G01N2021/6439 ,  C12Q2563/131 ,  C12Q2537/119 ,  C12Q2525/107

    摘要： The invention further relates to multichromophores, which may be conjugated polymers, and methods, articles and compositions employing them as described herein. In some aspects, the invention relates to methods, articles and compositions for the detection and analysis of biomolecules in a sample. Provided assays include those determining the presence of a target biomolecule in a sample or its relative amount, or the assays may be quantitative or semi-quantitative. The methods can be performed on a substrate. The methods can be performed in an array format on a substrate, which can be a sensor. In some embodiments, detection assays are provided employing sensor biomolecules that do not comprise a fluorophore that can exchange energy with the cationic multichromophore. In some aspects biological assays are provided in which energy is transferred between one or more of the multichromophore, a label on the target biomolecule, a label on the sensor biomolecule, and/or a fluorescent dye specific for a polynucleotide, in all permutations. The multichromophore may interact at least in part electrostatically with the sensor and/or the target, and an increase in energy transfer with the polymer may occur upon binding of the sensor and the target. Other variations of the inventions are described further herein.

    摘要翻译： 本发明还涉及可以是共轭聚合物的多色素，以及如本文所述使用它们的方法，制品和组合物。 在一些方面，本发明涉及用于检测和分析样品中生物分子的方法，制品和组合物。 提供的测定包括确定样品中靶生物分子的存在或其相对量的那些，或者测定可以是定量或半定量的。 该方法可以在衬底上进行。 这些方法可以以阵列格式在基底上进行，其可以是传感器。 在一些实施方案中，使用不包含能够与阳离子多重色素交换能量的荧光团的传感器生物分子来提供检测测定。 在一些方面，提供生物测定，其中在所有置换中，能量在一个或多个多色素，目标生物分子上的标记，传感器生物分子上的标记和/或多核苷酸特异的荧光染料之间转移。 多重色淀可以至少部分地与传感器和/或靶相互作用，并且当传感器和目标物结合时，聚合物的能量传递的增加可能发生。 本文进一步描述了本发明的其它变型。

    Aggregation sensor and solutions and kits comprising the same
    6.
    发明授权
    Aggregation sensor and solutions and kits comprising the same 有权
    聚合传感器和包含该聚合传感器的解决方案和套件

    公开(公告)号：US08110673B2

    公开(公告)日：2012-02-07

    申请号：US12711233

    申请日：2010-02-23

    申请人： Guillermo C. Bazan ,  Bin Liu

    发明人： Guillermo C. Bazan ,  Bin Liu

    IPC分类号： C07H21/04

    CPC分类号： G01N21/6486 ,  C08G61/02 ,  C08G61/123 ,  C12Q1/6818 ,  C12Q1/6825 ,  G01N33/542 ,  G01N33/54373 ,  Y10T436/143333 ,  C12Q2565/113 ,  C12Q2563/173 ,  C12Q2565/101

    摘要： This invention relates to an aggregation sensor useful for the detection and analysis of aggregants in a sample, and methods, articles and compositions relating to such a sensor. The sensor comprises first and second optically active units, where energy may be transferred from an excited state of the first optically active unit to the second optically active unit. The second optically active unit is present in a lesser amount, but its relative concentration is increased upon aggregation, increasing its absorption of energy from the first optically active units. This increase in energy transfer can be detected in variety of formats to produce an aggregation sensing system for various aggregants, including for quantitation. Other variations of the inventions are described further herein.

    摘要翻译： 本发明涉及可用于检测和分析样品中的聚集体的聚集传感器，以及与这种传感器相关的方法，制品和组合物。 传感器包括第一和第二光学有源单元，其中能量可以从第一光学活性单元的激发状态转移到第二光学活性单元。 第二光学活性单元以较小的量存在，但是其聚集时其相对浓度增加，从而增加其从第一光学活性单元的能量吸收。 可以以各种格式检测能量转移的这种增加，以产生用于各种聚合物的聚集感测系统，包括用于定量。 本文进一步描述了本发明的其它变型。

    Conjugated polymers suitable for strand-specific polynucleotide detection in homogeneous and solid state assays
    7.
    发明授权
    Conjugated polymers suitable for strand-specific polynucleotide detection in homogeneous and solid state assays 有权
    适用于均相和固态测定中的链特异性多核苷酸检测的共轭聚合物

    公开(公告)号：US07897684B2

    公开(公告)日：2011-03-01

    申请号：US11329495

    申请日：2006-01-10

    申请人： Guillermo C. Bazan ,  Bin Liu

    发明人： Guillermo C. Bazan ,  Bin Liu

    IPC分类号： C08G63/91 ,  C08G63/00 ,  C12Q1/68

    CPC分类号： C08G61/02 ,  C08G61/122 ,  C08G61/123 ,  C12Q1/6818 ,  C12Q1/6837 ,  G01N21/6428 ,  G01N21/6452 ,  G01N2021/6419 ,  G01N2021/6439 ,  C12Q2563/131 ,  C12Q2537/119 ,  C12Q2525/107

    摘要： The invention further relates to polycationic multichromophores, which may be conjugated polymers, and methods, articles and compositions employing them as described herein. In some aspects, the invention relates to methods, articles and compositions for the detection and analysis of biomolecules in a sample. Provided assays include those determining the presence of a target biomolecule in a sample or its relative amount, or the assays may be quantitative or semi-quantitative. The methods can be performed on a substrate. The methods can be performed in an array format on a substrate, which can be a sensor. In some embodiments, detection assays are provided employing sensor biomolecules that do not comprise a fluorophore that can exchange energy with the cationic multichromophore. In some aspects biological assays are provided in which energy is transferred between one or more of the multichromophore, a label on the target biomolecule, a label on the sensor biomolecule, and/or a fluorescent dye specific for a polynucleotide, in all permutations. The multichromophore may interact at least in part electrostatically with the sensor and/or the target, and an increase in energy transfer with the polymer may occur upon binding of the sensor and the target. Other variations of the inventions are described further herein.

    摘要翻译： 本发明还涉及可以是共轭聚合物的阳离子多色素，以及如本文所述使用它们的方法，制品和组合物。 在一些方面，本发明涉及用于检测和分析样品中生物分子的方法，制品和组合物。 提供的测定包括确定样品中靶生物分子的存在或其相对量的那些，或者测定可以是定量或半定量的。 该方法可以在衬底上进行。 这些方法可以以阵列格式在基底上进行，其可以是传感器。 在一些实施方案中，使用不包含能够与阳离子多重色素交换能量的荧光团的传感器生物分子来提供检测测定。 在一些方面，提供生物测定，其中在所有置换中，能量在一个或多个多色素，目标生物分子上的标记，传感器生物分子上的标记和/或多核苷酸特异的荧光染料之间转移。 多重色淀可以至少部分地与传感器和/或靶相互作用，并且当传感器和目标物结合时，聚合物的能量传递的增加可能发生。 本文进一步描述了本发明的其它变型。

    Structures incorporating conformationally flexible conjugated polymers and methods of use
    9.
    发明授权
    Structures incorporating conformationally flexible conjugated polymers and methods of use 有权
    包含构象柔性共轭聚合物和使用方法的结构

    公开(公告)号：US09159465B2

    公开(公告)日：2015-10-13

    申请号：US13075172

    申请日：2011-03-29

    申请人： Guillermo C. Bazan ,  Bin Liu

    发明人： Guillermo C. Bazan ,  Bin Liu

    IPC分类号： C40B40/04 ,  H01B1/12 ,  C08G61/02 ,  C08G61/10 ,  C08G61/12 ,  C08G73/02 ,  C08G73/08

    CPC分类号： H01B1/122 ,  C08G61/02 ,  C08G61/10 ,  C08G61/123 ,  C08G61/126 ,  C08G73/02 ,  C08G73/08 ,  C40B40/04

    摘要： Methods, compositions and articles of manufacture involving conformationally flexible conjugated polymers are provided. A structure is provided comprising the conformationally flexible conjugated polymer bound to or associated with at least one member of a binding pair comprising a sensor molecule and a target molecule or the complex they form. The conformationally flexible conjugated polymer comprises at least one angled linker having bonds to its two adjacent polymeric units which form an angle of less than about 155° with respect to one another. Methods of use of such structures and solutions comprising them are also provided.

    摘要翻译： 提供涉及构象柔性共轭聚合物的方法，组合物和制品。 提供了包含结构柔性共轭聚合物结构或与至少一个包含传感器分子和靶分子或其形成的复合物的结合对成员相关联的结构。 构象柔性的共轭聚合物包括至少一个成角度的连接体，其具有与其两个相邻的聚合物单元的键合，其相对于彼此形成小于约155°的角度。 还提供了使用这种结构的方法和包括它们的溶液。