公开(公告)号：US20160209403A1

公开(公告)日：2016-07-21

申请号：US14914278

申请日：2014-11-26

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Ryosuke TAKAHASHI ,  Katsuhiro KANDA ,  Yusuke SHIMIZU ,  Hisashi ICHIKAWA

IPC: G01N33/50 ,  C12M1/34 ,  C12M1/00 ,  G01N33/15 ,  C12M1/12

CPC classification number: G01N33/5067 ,  B01L3/5085 ,  C12M25/00 ,  C12M29/00 ,  C12M41/46 ,  C12M47/20 ,  G01N33/15 ,  G01N33/502 ,  G01N2035/00425 ,  G01N2035/0418

Abstract: Application of the present invention enables quantification of fractions of candidate pharmaceutical compounds (a parent compound and its metabolites), one excreted to the basolateral (Basal/Basolateral)-side via transporters and by diffusion, one excreted to the lumen (Apical)-side, and one remained in the cells. This enables determination of the total amount of the administered candidate pharmaceutical compounds and the distribution ratio of the fractions. The kinetics of the administered candidate pharmaceutical compounds can be evaluated, thereby enabling in vitro screening of an enormous number of candidate pharmaceutical compounds for drug candidates exhibiting the efficacy. The object of the present invention is to provide an apparatus and method for understanding a total picture of pharmacokinetics in vitro by quantifying a fraction of basolateral (Basal/Basolateral) efflux, a fraction of lumen (Apical)-side excretion, and a fraction remaining in a cell of a drug which has been administered to the cell to determine the distribution ratio of each fraction.

Abstract translation: 本发明的应用使得能够定量分离出候选药物化合物（母体化合物及其代谢物），一种通过转运蛋白和扩散物排泄到基底外侧（基底/基底外侧），一个排泄到内腔（Apical） ，还有一个留在细胞中。 这使得能够确定所施用的候选药物化合物的总量和分数的分配比。 可以评估给药的候选药物化合物的动力学，从而能够体外筛选展示功效的药物候选药物的大量候选药物化合物。 本发明的目的是提供一种用于通过量化基底外侧（基底/基底外侧）流出的一部分（一部分管腔（Apical）侧排泄物）和剩余部分来了解体外药代动力学总体图像的装置和方法 在已经施用于细胞以确定每个级分的分配比例的药物的细胞中。

公开(公告)号：US20180106783A1

公开(公告)日：2018-04-19

申请号：US15847092

申请日：2017-12-19

Applicant: Hitachi High-Technologies Corporation

Inventor： Ryosuke TAKAHASHI ,  Katsuhiro KANDA ,  Yusuke SHIMIZU ,  Hisashi ICHIKAWA

IPC: G01N33/50 ,  G01N33/15 ,  C12M1/00 ,  C12M1/12 ,  C12M1/34 ,  G01N35/00 ,  G01N35/04

Abstract: Application of the present invention enables quantification of fractions of candidate pharmaceutical compounds (a parent compound and its metabolites), one excreted to the basolateral (Basal/Basolateral)-side via transporters and by diffusion, one excreted to the lumen (Apical)-side, and one remained in the cells. This enables determination of the total amount of the administered candidate pharmaceutical compounds and the distribution ratio of the fractions. The kinetics of the administered candidate pharmaceutical compounds can be evaluated, thereby enabling in vitro screening of an enormous number of candidate pharmaceutical compounds for drug candidates exhibiting the efficacy. The object of the present invention is to provide an apparatus and method for understanding a total picture of pharmacokinetics in vitro by quantifying a fraction of basolateral (Basal/Basolateral) efflux, a fraction of lumen (Apical)-side excretion, and a fraction remaining in a cell of a drug which has been administered to the cell to determine the distribution ratio of each fraction.

公开(公告)号：US20180120296A1

公开(公告)日：2018-05-03

申请号：US15565959

申请日：2016-01-05

Applicant: Hitachi High-Technologies Corporation

Inventor： Ryosuke TAKAHASHI ,  Katsuhiro KANDA ,  Yusuke SHIMIZU

IPC: G01N33/50

Abstract: Provided is a component analysis device, which comprises an analysis unit for measuring a component fed to the plurality of containers and analyzing the component thus measured, wherein the plurality of containers are at least a first container and a second container, wherein the first container retains a first solution containing a substance that releases adhesion between multiple cells forming the liver cell tissue, and the second container retains a buffer solution, and wherein the analysis unit measures an amount of the component discharged from the liver cell tissue in the first container into the first solution and an amount of the component discharged from the liver cell tissue in the second container into the buffer solution in the second container, and analyzes an amount of the component to be discharged via a bile duct in the liver cell tissue.

公开(公告)号：US20150111300A1

公开(公告)日：2015-04-23

申请号：US14578619

申请日：2014-12-22

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Makoto NOGAMI ,  Katsuhiro KANDA ,  Izumi WAKI

IPC: G01N1/40 ,  G01N35/00 ,  G01N35/02

CPC classification number: G01N1/405 ,  G01N35/00663 ,  G01N35/02 ,  G01N35/025 ,  Y10T436/115831

Abstract: A clinical laboratory apparatus includes a pretreating apparatus having a high separation capability and providing high data reproducibility and reliability and a mass spectrometer is provided which executes fully automatic processing from pretreatment to detection. The pretreating apparatus that includes a cartridge for holding an extraction agent for solid-phase extraction, a pressure-loading unit for applying a pressure load to any cartridge mounted on the solid-phase extraction cartridge-retaining unit, a receiving tray mechanism for receiving a sample extracted from the cartridge, and a liquid-level sensor for detecting liquid levels in the receiving tray as well as in the cartridge, conducts feedback control for the pressure-loading unit to open its pressure release valve upon the liquid-level sensor detecting that the liquid level in the receiving tray mechanism has reached a preset liquid-level position.

Abstract translation: 临床实验室装置包括具有高分离能力并且提供高数据再现性和可靠性的预处理装置，并且提供执行从预处理到检测的全自动处理的质谱仪。 该预处理装置包括用于保持固相萃取提取剂的盒，用于对安装在固相提取盒保持单元上的任何盒施加压力负载的压力加载单元，用于接收 从墨盒提取的样品和用于检测接收托盘以及墨盒中的液位的液位传感器，对液位传感器检测到压力加载单元进行反馈控制以打开其压力释放阀， 接收托盘机构中的液位达到预设液位位置。

公开(公告)号：US20150064739A1

公开(公告)日：2015-03-05

申请号：US14535424

申请日：2014-11-07

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Makoto NOGAMI ,  Yuichiro HASHIMOTO ,  Izumi WAKI ,  Katsuhiro KANDA

IPC: G01N33/49

CPC classification number: G01N33/49 ,  G01N1/4055 ,  G01N2001/4083 ,  H01J49/0009 ,  H01J49/0031 ,  H01J49/0036 ,  H01J49/0436

Abstract: An analyzer includes a pretreatment device and a mass spectrometer. The pretreatment device includes a solid phase extraction mechanism. The mass spectrometer performs mass spectrometry on a sample pretreated by the pretreatment device and then subjected to ionization. The analyzer also includes a storage unit that stores data on dependence of signal intensities of the analyte substance to be measured and the internal standard upon the concentration of a substance inhibiting ionization in the sample, and that stores data on a recovery rate. The analyzer also includes a correcting unit that corrects measurement results of the sample and the internal standard on the basis of the data stored in the storage unit.

Abstract translation: 分析仪包括预处理装置和质谱仪。 预处理装置包括固相提取机构。 质谱仪对由预处理装置预处理的样品进行质谱分析，然后进行电离。 分析仪还包括存储单元，其存储关于待测量的待分析物质的信号强度与内标的数据，所述数据在样品中抑制电离物质的浓度，并且存储关于回收率的数据。 分析器还包括校正单元，其基于存储在存储单元中的数据来校正样本的测量结果和内部标准。

公开(公告)号：US20130075603A1

公开(公告)日：2013-03-28

申请号：US13680630

申请日：2012-11-19

Applicant: Hitachi High-Technologies Corporation

Inventor： Katsuhiro KANDA ,  Makoto NOGAMI ,  Izumi WAKI

IPC: G01N1/40 ,  H01J49/26

CPC classification number: G01N1/4077 ,  G01N1/405 ,  G01N30/82 ,  G01N2030/009 ,  H01J49/0431 ,  H01J49/26

Abstract: The present invention relates to a pretreatment apparatus that performs concentration and separation of a sample, and in particular, in order to provide a sample pretreatment apparatus using a solid-phase extraction column, and a mass spectrometer using the same, which is particularly suitable for clinical analysis in which qualitative/quantitative analysis of a biological sample such as blood is performed, according to each operational step for a solid-phase extraction treatment, for example, a collection device serving as flow passages or containers for collection of waste liquid or extracted matter is installed on a bottom face of the solid-phase extraction column, and the extracted matter is separately collected without being mixed with waste liquid by switching the positions of the collection device.

Abstract translation: 本发明涉及一种进行样品浓缩分离的预处理装置，特别是为了提供使用固相萃取柱的样品预处理装置和使用其的质谱仪，特别适用于 根据固相萃取处理的每个操作步骤，例如用作流动通道的收集装置或用于收集废液或提取的容器的容器，进行诸如血液的生物样品的定性/定量分析的临床分析 物质被安装在固相萃取塔的底面上，并且通过切换收集装置的位置而分离地收集提取物而不与废液混合。