摘要:
A method of isolating a lactic acid bacterial DNA fragment comprising a promoter, the method comprising introducing a DNA molecule comprising a transposable element comprising a promoterless structural gene as a promoter probe gene into a population of a lactic acid bacterium, methods of constructing a recombinant lactic acid bacterium comprising a regulatable promoter by using the above method, a recombinant lactic acid bacterium comprising a gene coding for a desired gene product and operably linked thereto a regulatable lactic acid bacterial promoter not natively associated with the gene, the use of such a recombinant lactic acid bacterium and recombinant plasmids comprising a regulatable lactic acid bacterial promoter.
摘要:
Methods of isolating nonsense suppressor-encoding lactic acid bacteria, cultures and compositions of lactic acid bacteria and plasmids comprising a gene coding for a nonsense suppressor, and a method of confining extrachromosomal replicons capable of replicating in lactic acid bacteria to lactic acid bacteria.
摘要:
Useful mutants of lactic acid bacteria or plasmids capable of replicating in lactic acid bacteria, comprising nonsense mutation suppressor-encoding genes, the use of such suppressor genes for confining a replicon to a specific lactic acid bacterium or to a lactic acid bacterium growing in a particular environment and for controlling the number of lactic acid bacterial cells in a particular environment.
摘要:
The invention relates to methods for producing a fermentation product from a lignocellulose-containing material comprising i) pre-treating lignocellulose-containing material; ii) hydrolysing pre-treated lignocellulose-containing material; iii) fermenting using a fermenting organism; wherein fermentation is initiated and carried out at: a) a fermentation organism cell count in the range from 10-250×1010 cells per L fermentation medium; or b) a fermentation organism concentration in the range from 2-90 g dry weight fermenting organism per L fermentation medium.
摘要:
A drive wheel for off-center drive of an annular saw blade in a cutting machine, consists of at least two components, which are separable but held together during operation, namely a holding disc and a drive ring, the latter having an encircling drive groove for the inner edge of the saw blade, the drive ring being arranged radially outside a central portion of the holder disc.
摘要:
A plunge valve for closing and for opening a connection in a hydraulic conduit comprises a plunge (37) which is slidably movable in the hydraulic conduit (5, 5A) under the influence of the pressure in a section (5A) of the conduit on the inlet side of said conduit adjacent to the plunge between a first, open position, when the pressure in said section (5A) is high, in which position the plunge is withdrawn and communication is established, and a second, closed position, in which the pressure in said section is low, in which position the plunge shuts off said connection.
摘要:
Method of preventing bacteriophage infection of bacterial cultures comprising modified strains, wherein the cultures are completely resistant to bacteriophage attack and have retained their capability of being metabolically active. The method is useful in the manufacturing of food products, feed products or useful metabolite products.
摘要:
A robot for medical ultrasonic examination has an articulated robot arm with a plurality of arm units (14-16), mounted one on the other to be pivotable, and a computerized system for controlling the movements of the arm, the outermost arm unit being arranged to carry a probe. The outermost arm unit has a carrying member rotatable about a longitudinal axis (IV), and carries a probe holder pivotable about a transverse axis (V) perpendicular to the longitudinal axis (IV). The probe holder has a housing rotatable about the transverse axis, and a holder sleeve rotatable about a longitudinal axis (VI). The three axes (IV, V, VI) intersect at a single point. The probe holder has an opening through which the probe can be inserted from the back and locked in position in the probe holder.
摘要:
A robot for medical ultrasonic examination has an articulated robot arm with a plurality of arm units (14-16), mounted one on the other to be pivotable, and a computerized system for controlling the movements of the arm, the outermost arm unit being arranged to carry a probe. The outermost arm unit has a carrying member rotatable about a longitudinal axis (IV), and carries a probe holder pivotable about a transverse axis (V) perpendicular to the longitudinal axis (IV). The probe holder has a housing rotatable about the transverse axis, and a holder sleeve rotatable about a longitudinal axis (VI). The three axes (IV, V, VI) intersect at a single point. The probe holder has an opening through which the probe can be inserted from the back and locked in position in the probe holder.
摘要:
The invention relates to methods for producing a fermentation product from a lignocellulose-containing material comprising i) pre-treating lignocellulose-containing material; ii) hydrolyzing pre-treated lignocellulose-containing material; iii) fermenting using a fermenting organism; wherein fermentation is initiated and carried out at: a) a fermentation organism cell count in the range from 10-250×1010 cells per L fermentation medium; or b) a fermentation organism concentration in the range from 2-90 g dry weight fermenting organism per L fermentation medium.