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    Food-grade cloning vector and their use in lactic acid bacteria
    2.
    发明授权
    Food-grade cloning vector and their use in lactic acid bacteria 失效
    食品级克隆载体及其在乳酸菌中的应用

    公开(公告)号：US07358083B1

    公开(公告)日：2008-04-15

    申请号：US09673617

    申请日：1999-04-14

    Applicant: Kim Sorensen ,  Rasmus Larsen ,  Eric Johansen

    Inventor： Kim Sorensen ,  Rasmus Larsen ,  Eric Johansen

    IPC: C12N15/74 ,  C12N1/21 ,  C12N15/68 ,  A23C9/123

    CPC classification number: C12N15/746 ,  A23C9/123 ,  A23C2220/202

    Abstract: Novel food-grade cloning vectors comprising a nonsense mutation suppressor-encoding gene, which vector, when it is present in a lactic acid bacterial strain, permits such a strain to have an industrially appropriate growth rate and metabolic activity. The cloning vectors are useful when present in lactic acid bacteria used as starter cultures in the preparation of food or feed products, or a dairy flavor.

    Abstract translation: 包含无义突变抑制子编码基因的新型食品级克隆载体，当该载体存在于乳酸菌株中时，该载体允许这种菌株具有工业上合适的生长速率和代谢活性。 当存在于用作食品或饲料产品或乳制品风味剂的起始培养物的乳酸菌中时，克隆载体是有用的。

    Recombinant lactic acid bacterium containing an inserted promoter and method of constructing same
    3.
    发明授权
    Recombinant lactic acid bacterium containing an inserted promoter and method of constructing same 失效
    含有插入启动子的重组乳酸菌及其构建方法

    公开(公告)号：US5837509A

    公开(公告)日：1998-11-17

    申请号：US179557

    申请日：1995-03-01

    Applicant: Hans Israelsen ,  Egon Bech Hansen ,  Eric Johansen ,  Soeren Michael Madsen ,  Dan Nilsson ,  Astrid Vrang

    Inventor： Hans Israelsen ,  Egon Bech Hansen ,  Eric Johansen ,  Soeren Michael Madsen ,  Dan Nilsson ,  Astrid Vrang

    IPC: C12N1/21 ,  C12N15/74 ,  C12N15/77 ,  C12N15/11 ,  C12P21/02

    CPC classification number: C12N15/746 ,  C12N15/77 ,  Y10S435/853

    Abstract: A method of isolating a lactic acid bacterial DNA fragment comprising a promoter, the method comprising introducing a DNA molecule comprising a transposable element comprising a promoterless structural gene as a promoter probe gene into a population of a lactic acid bacterium, methods of constructing a recombinant lactic acid bacterium comprising a regulatable promoter by using the above method, a recombinant lactic acid bacterium comprising a gene coding for a desired gene product and operably linked thereto a regulatable lactic acid bacterial promoter not natively associated with the gene, the use of such a recombinant lactic acid bacterium and recombinant plasmids comprising a regulatable lactic acid bacterial promoter.

    Abstract translation: PCT No.PCT / DK94 / 00004 Sec。 371 1995年3月1日 102（e）1995年3月1日PCT 1994年3月1日PCT PCT。 WO94 / 16086 PCT出版物 日期：1994年7月21日一种分离含有启动子的乳酸菌DNA片段的方法，该方法包括将包含无启动子结构基因的转座元件作为启动子探针基因的DNA分子引入乳酸菌群中， 通过使用上述方法构建包含可调节启动子的重组乳酸菌，重组乳酸菌，其包含编码所需基因产物的基因，并与其可操作地连接与该基因无天然关联的可调节乳酸细菌启动子，使用 的这种重组乳酸菌和包含可调节的乳酸细菌启动子的重组质粒。

    TEXTURIZING LACTIC ACID BACTERIA STRAINS
    8.
    发明申请
    TEXTURIZING LACTIC ACID BACTERIA STRAINS 有权
    纹理酸性细菌菌株

    公开(公告)号：US20130344199A1

    公开(公告)日：2013-12-26

    申请号：US13880620

    申请日：2011-10-21

    Applicant: Annette Kibenich ,  Kim Ib Soerensen ,  Eric Johansen

    Inventor： Annette Kibenich ,  Kim Ib Soerensen ,  Eric Johansen

    IPC: C12N15/74 ,  A23C9/123

    CPC classification number: C12N1/20 ,  A23C9/1238 ,  A23Y2220/15 ,  A23Y2220/29 ,  A23Y2240/75 ,  C12N15/01 ,  C12N15/746 ,  C12R1/225 ,  C12R1/46

    Abstract: The present invention relates to mutants of lactic acid bacteria which are resistant towards the antibiotic D-cycloserine and/or functionally equivalent antibiotics and which were found to give an increased texture when grown in milk while maintaining the other growth properties of the parent strain. The present invention, furthermore, relates to compositions comprising such mutants, and to dairy products fermented with the lactic acid bacteria resistant towards D-cycloserine and/or functionally equivalent antibiotics.

    Abstract translation: 本发明涉及对抗生素D-环丝氨酸和/或功能相当的抗生素具有抗性的乳酸菌突变体，并且在保持母体菌株的其它生长特性的同时，在牛奶中生长时，其质地增加。 此外，本发明涉及包含这种突变体的组合物，以及用对D-环丝氨酸和/或功能等同的抗生素具有抗性的乳酸菌发酵的乳制品。

    Method for selecting mutants of legume-nodulating bacteria enhanced in competition
    9.
    发明授权
    Method for selecting mutants of legume-nodulating bacteria enhanced in competition 失效
    在竞争中增强豆科结瘤细菌突变体的选择方法

    公开(公告)号：US5432079A

    公开(公告)日：1995-07-11

    申请号：US166681

    申请日：1988-03-11

    Applicant: Eric Johansen ,  Edward R. Appelbaum

    Inventor： Eric Johansen ,  Edward R. Appelbaum

    IPC: C12N15/74 ,  C12Q1/04 ,  C12Q1/68 ,  C12N1/20 ,  C12N1/21 ,  C12N15/01

    CPC classification number: C12Q1/6897 ,  C12N15/743 ,  C12Q1/04 ,  Y10S435/878

    Abstract: Methods for the isolation of mutant rhizobial strains with improved competition of nodulation (Comp.sup.+) is presented. Mutants are selected for constitutive expression of inducible nod genes (in the absence of inducer) and screened for hyperinduction of nod genes in the presence of inducer. Mutants which are defective in nodulation or in symbiotic nitrogen fixation are eliminated from further testing. Selections for constitutive nod expression and for hyperinduction of nod genes are facilitated by the use of plasmid carrying a selectable marker fused to an inducible nod gene and a plasmid carrying a reporter gene fused downstream of an inducible nod gene. The methods exemplified are particularly useful for the isolation and identification of Comp.sup.+ mutants of Bradyrhizobium japonicum.

    Abstract translation: 提出了具有改善的结瘤竞争（Comp +）的突变根瘤菌菌株分离方法。 选择突变体用于诱导型节点基因的组成型表达（在不存在诱导物的情况下），并筛选出在诱导物存在下点头基因的高度诱导。 在结瘤或共生固氮中有缺陷的突变体从进一步测试中消除。 通过使用携带可诱导的点头基因融合的选择标记的质粒和携带可诱导的点头基因下游融合的报道基因的质粒，促进了组成型nod表达和nod基因超调诱导的选择。 所列举的方法对于分离和鉴定日本Bradyrhizobium日本的Comp +突变体是特别有用的。

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