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公开(公告)号：US06916616B2
公开(公告)日：2005-07-12
申请号：US10025826
申请日：2001-12-19
CPC分类号： C12Q1/686 , C12Q1/68 , C12Q1/6806 , C12Q1/6846 , C12Q1/6869 , C12Q2527/125 , C12Q2527/119 , C12Q2535/113
摘要： The invention concerns stable aqueous solutions containing one or several nucleoside triphosphates wherein the respective solution has a pH value of more than 7.5 and contains no additional substances with a stabilizing effect. The nucleoside triphosphate solutions are used in particular for DNA synthesizing reactions such as e.g. RT-PCR, cycle sequencing, random priming and nick translation. One of the most important applications of such solutions containing deoxy-nucleoside triphosphates (d-NTP) is their use in the polymerase chain reaction (PCR).
摘要翻译： 本发明涉及含有一种或多种核苷三磷酸的稳定的水溶液，其中各自的溶液的pH值大于7.5，并且不含有具有稳定作用的其它物质。 核苷三磷酸溶液特别用于DNA合成反应，例如 RT-PCR，循环测序，随机引发和切口平移。 含有脱氧核苷三磷酸（d-NTP）的这种溶液最重要的应用之一是它们在聚合酶链反应（PCR）中的应用。
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公开(公告)号：US20080280329A1
公开(公告)日：2008-11-13
申请号：US11799983
申请日：2007-05-03
申请人： Wilhelm Tischer , Hans-Georg Ihlenfeldt , Octavian Barzu , Hiroshi Sakamoto , Elisabeth Pistotnik , Philippe Marliere , Sylvie Pochet
发明人： Wilhelm Tischer , Hans-Georg Ihlenfeldt , Octavian Barzu , Hiroshi Sakamoto , Elisabeth Pistotnik , Philippe Marliere , Sylvie Pochet
IPC分类号： C12P19/34
CPC分类号： C12N9/0006 , C12N9/1051 , C12N9/1077 , C12N9/1205 , C12N9/88 , C12N9/90 , C12P9/00 , C12P19/02 , C12P19/24 , C12P19/38
摘要： The present invention relates to a method for the in vitro synthesis of deoxyribonucleosides and enzymes suitable for this method.
摘要翻译： 本发明涉及一种适用于该方法的体外合成脱氧核糖核苷和酶的方法。
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公开(公告)号：US5935778A
公开(公告)日：1999-08-10
申请号：US845926
申请日：1997-04-28
申请人： Cristoph Seidel , Ursula-Henrike Wienhues-Thelen , Urban Schmitt , Gunther-Gerhard Jung , Hans-Georg Ihlenfeldt , Wolfgang Kraas
发明人： Cristoph Seidel , Ursula-Henrike Wienhues-Thelen , Urban Schmitt , Gunther-Gerhard Jung , Hans-Georg Ihlenfeldt , Wolfgang Kraas
IPC分类号： G01N33/53 , C07K7/08 , C07K14/18 , G01N33/576 , C12Q1/70
CPC分类号： C07K14/005 , G01N33/53 , G01N33/5767 , C12N2770/24222 , Y10S436/82
摘要： The invention concerns a method for typing antibodies in a sample liquid by means of type-specific antigens and in particular a method for typing antibodies to the hepatitis C virus and peptide antigens suitable for this.
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4.发明授权Recombinant kinase from insect cells for the synthesis of nucleoside monophosphates 有权来自昆虫细胞的重组激酶用于合成核苷单磷酸公开(公告)号：US07625735B2
公开(公告)日：2009-12-01
申请号：US10680635
申请日：2003-10-07
CPC分类号： C12N9/1205
摘要： A recombinant Drosophila melanogaster deoxynucleoside kinase that remains stable during the synthesis of nucleoside monophosphate without the addition of stabilizing SH reagents or stabilizing proteins, and that accepts all four natural deoxynucleotides is provided. In addition, the invention concerns DNA sequences, vectors, transformed cells, a method for production of the recombinant kinase as well as its use for preparing nucleoside monophosphates.
摘要翻译： 提供了一种重组黑腹果蝇脱氧核苷激酶，其在合成核苷单磷酸酯期间保持稳定，而不添加稳定的SH试剂或稳定蛋白，并且接受所有四种天然脱氧核苷酸。 此外，本发明涉及DNA序列，载体，转化细胞，重组激酶的制备方法及其制备核苷单磷酸的用途。
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公开(公告)号：US07229797B1
公开(公告)日：2007-06-12
申请号：US10049750
申请日：2000-08-18
申请人： Wilhelm Tischer , Hans-Georg Ihlenfeldt , Octavian Barzu , Hiroshi Sakamoto , Elisabeth Pistotnik , Philippe Marlière , Sylvie Pochet
发明人： Wilhelm Tischer , Hans-Georg Ihlenfeldt , Octavian Barzu , Hiroshi Sakamoto , Elisabeth Pistotnik , Philippe Marlière , Sylvie Pochet
CPC分类号： C12N9/0006 , C12N9/1051 , C12N9/1077 , C12N9/1205 , C12N9/88 , C12N9/90 , C12P9/00 , C12P19/02 , C12P19/24 , C12P19/38
摘要： The present invention relates to a method for the in vitro enzymatic synthesis of deoxyribonucleosides and enzymes suitable for this method.
摘要翻译： 本发明涉及一种适用于该方法的体外酶合成脱氧核糖核苷和酶的方法。
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6.发明申请Deoxynucleoside kinase from insect cells for the synthesis of nucleoside monophosphates 有权来自昆虫细胞的脱氧核苷激酶用于合成核苷单磷酸酯公开(公告)号：US20060252131A1
公开(公告)日：2006-11-09
申请号：US10680635
申请日：2003-10-07
CPC分类号： C12N9/1205
摘要： Recombinant kinase remaining stable during the synthesis of necleoside monophosphate without the addition of stabilizing SH reagents, without stabilizing proteins and accepting all four natural deoxynucleotides, obtainable from insect cells such as e.g. Drosophila Melanogaster. In addition, the invention concerns DNA sequences, vectors, transformed cells, a method for production of the recombinant kinase as well as its use for preparing nucleoside monophosphates.
摘要翻译： 重组激酶在合成核苷酸单磷酸酯期间保持稳定，而不添加稳定的SH试剂，而不稳定蛋白质并接受可从昆虫细胞获得的所有四种天然脱氧核苷酸。 果蝇Melanogaster。 此外，本发明涉及DNA序列，载体，转化细胞，重组激酶的制备方法及其制备核苷单磷酸的用途。
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公开(公告)号：US06447992B1
公开(公告)日：2002-09-10
申请号：US09351296
申请日：1999-07-12
申请人： Cristoph Seidel , Ursula-Henrike Wienhues-Thelen , Urban Schmitt , Günther-Gerhard Jung , Hans-Georg Ihlenfeldt , Wolfgang Kraas
发明人： Cristoph Seidel , Ursula-Henrike Wienhues-Thelen , Urban Schmitt , Günther-Gerhard Jung , Hans-Georg Ihlenfeldt , Wolfgang Kraas
IPC分类号： A61K3929
CPC分类号： C07K14/005 , C12N2770/24222 , G01N33/53 , G01N33/5767 , Y10S436/82
摘要： The invention concerns a method for typing antibodies in a sample liquid by means of type-specific antigens and in particular a method for typing antibodies to the hepatitis C virus and peptide antigens suitable for this.
摘要翻译： 本发明涉及通过类型特异性抗原在样品液体中分型抗体的方法，特别是针对丙型肝炎病毒和适合于其的肽抗原分型抗体的方法。
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