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1.发明授权T-structure invasive cleavage assays, consistent nucleic acid dispensing, and low level target nucleic acid detection 有权T结构侵入性切割测定，一致的核酸分配和低水平靶核酸检测公开(公告)号：US08354232B2
公开(公告)日：2013-01-15
申请号：US12840015
申请日：2010-07-20
申请人： Hatim Taysir Allawi , Vecheslav A. Elagin , Victor I. Lyamichev , Kwok Wu , Walter Iszczyszyn , Chris Fleming , LuAnne Chehak , Scott M. Law
发明人： Hatim Taysir Allawi , Vecheslav A. Elagin , Victor I. Lyamichev , Kwok Wu , Walter Iszczyszyn , Chris Fleming , LuAnne Chehak , Scott M. Law
CPC分类号： C12Q1/6823 , C12Q1/70 , C12Q2521/301 , C12Q2525/301
摘要： The present invention relates to systems, methods and kits for low-level detection of nucleic acids, detecting at least two different viral sequences in a single reaction vessel, and increasing the dynamic range of detection of a viral target nucleic acid in a sample. The present invention also relates to T-structure invasive cleavage assays, as well as T-structure related target dependent non-target amplification methods and compositions. The present invention further relates to methods, compositions, devices and systems for consistent nucleic acid dispensing onto surfaces.
摘要翻译： 本发明涉及用于低水平检测核酸的系统，方法和试剂盒，在单个反应容器中检测至少两种不同的病毒序列，并增加样品中病毒靶核酸的动态检测范围。 本发明还涉及T结构侵入性切割测定，以及T结构相关靶依赖性非靶扩增方法和组合物。 本发明还涉及用于将一致的核酸分配到表面上的方法，组合物，装置和系统。
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公开(公告)号：US06759226B1
公开(公告)日：2004-07-06
申请号：US09577304
申请日：2000-05-24
申请人： Wu-Po Ma , Victor I. Lyamichev , Michael W. Kaiser , Natalie E. Lyamicheva , Hatim Taysir Allawi , James J. Schaefer , Bruce P. Neri
发明人： Wu-Po Ma , Victor I. Lyamichev , Michael W. Kaiser , Natalie E. Lyamicheva , Hatim Taysir Allawi , James J. Schaefer , Bruce P. Neri
IPC分类号： C12N922
CPC分类号： C12Q1/6823 , C12N9/1252 , C12N9/22 , C12Q1/683 , Y10T436/143333 , C12Q2561/109
摘要： The present invention relates to novel enzymes designed for direct detection, characterization and quantitation of nucleic acids, particularly RNA. The present invention provides enzymes that recognize specific nucleic acid cleavage structures formed on a target RNA sequence and that cleave the nucleic acid cleavage structure in a site-specific manner to produce non-target cleavage products. The present invention provides enzymes having an improved ability to specifically cleave a DNA member of a complex comprising DNA and RNA nucleic acid strands.
摘要翻译： 本发明涉及设计用于直接检测，表征和定量核酸，特别是RNA的新型酶。 本发明提供了识别在靶RNA序列上形成的特异性核酸切割结构并以位点特异性方式切割核酸切割结构以产生非靶切割产物的酶。 本发明提供具有改进的能够特异性切割包含DNA和RNA核酸链的复合物的DNA成员的酶的酶。
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公开(公告)号：US06635463B2
公开(公告)日：2003-10-21
申请号：US09758282
申请日：2001-01-11
申请人： Wu-Po Ma , Victor I. Lyamichev , Michael W. Kaiser , Natalie E. Lyamicheva , Hatim Taysir Allawi , James J. Schaefer , Bruce P. Neri
发明人： Wu-Po Ma , Victor I. Lyamichev , Michael W. Kaiser , Natalie E. Lyamicheva , Hatim Taysir Allawi , James J. Schaefer , Bruce P. Neri
IPC分类号： C12N922
CPC分类号： C12Q1/6823 , C12N9/1252 , C12N9/22 , C12Q1/683 , Y10T436/143333 , C12Q2561/109
摘要： The present invention relates to novel enzymes designed for direct detection, characterization and quantitation of nucleic acids, particularly RNA. The present invention provides enzymes that recognize specific nucleic acid cleavage structures formed on a target RNA sequence and that cleave the nucleic acid cleavage structure in a site-specific manner to produce non-target cleavage products. The present invention provides enzymes having an improved ability to specifically cleave a DNA member of a complex comprising DNA and RNA nucleic acid strands.
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公开(公告)号：US07824862B2
公开(公告)日：2010-11-02
申请号：US12242508
申请日：2008-09-30
申请人： Fang Dong , Victor I. Lyamichev , James R. Prudent , Lance Fors , Bruce P. Neri , Mary Ann D. Brow , Todd A. Anderson , James E. Dahlberg
发明人： Fang Dong , Victor I. Lyamichev , James R. Prudent , Lance Fors , Bruce P. Neri , Mary Ann D. Brow , Todd A. Anderson , James E. Dahlberg
CPC分类号： C12Q1/6832 , C12Q1/701 , C12Q2565/133 , C12Q2521/301
摘要： The present invention relates to methods and compositions for analyzing nucleic acids. In particular, the present invention provides methods and compositions for the detection and characterization of nucleic acid sequences and sequence changes. The methods of the present invention permit the detection and/or identification of genetic polymorphism such as those associated with human disease and permit the identification of pathogens (e.g., viral and bacterial strain identification).
摘要翻译： 本发明涉及用于分析核酸的方法和组合物。 特别地，本发明提供了用于检测和表征核酸序列和序列变化的方法和组合物。 本发明的方法允许检测和/或鉴定遗传多态性，例如与人类疾病相关的遗传多态性，并允许鉴定病原体（例如病毒和细菌菌株鉴定）。
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公开(公告)号：US07541145B2
公开(公告)日：2009-06-02
申请号：US11183078
申请日：2005-07-15
CPC分类号： C12N9/22 , C12Q1/6823 , C12Q1/6827 , C12Q1/683 , Y10S435/81 , Y10S435/822 , C12Q2565/525 , C12Q2561/109 , C12Q2525/301 , C12Q2563/149
摘要： The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译： 本发明涉及核酸序列的检测和表征以及核酸序列的变化的方法。 本发明还涉及在靶序列上形成核酸切割结构并以位点特异性方式切割核酸切割结构的方法。 使用各种酶的结构特异性核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。
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公开(公告)号：US20080188375A1
公开(公告)日：2008-08-07
申请号：US11926120
申请日：2007-10-28
CPC分类号： C12N9/22 , C12Q1/6823 , C12Q1/6827 , Y10S435/81 , Y10S435/822 , C12Q2565/525 , C12Q2561/109 , C12Q2525/301
摘要： The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on a solid support and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5′ nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译： 本发明涉及核酸序列的检测和表征的组合物和方法以及核酸序列的变化。 本发明涉及在固体支持物上形成核酸切割结构并以位点特异性方式切割核酸切割结构的方法。 例如，在一些实施方案中，使用来自任何多种酶的5'核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。
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公开(公告)号：US07122364B1
公开(公告)日：2006-10-17
申请号：US09714935
申请日：2000-11-17
CPC分类号： C12N9/22 , C12Q1/6813 , C12Q2521/301
摘要： The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5′ nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
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公开(公告)号：US06555357B1
公开(公告)日：2003-04-29
申请号：US09684938
申请日：2000-10-06
IPC分类号： C12N1555
CPC分类号： C12N9/22 , C12Q1/6823 , Y10S435/975 , C12Q2525/301 , C12Q2561/109
摘要： The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to improved cleavage means for the detection and characterization of nucleic acid sequences. Structure-specific nucleases derived from a variety of thermostable organisms are provided. These structure-specific nucleases are used to cleave target-dependent cleavage structures, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译： 本发明涉及核酸序列的检测和表征以及核酸序列的变化的方法。 本发明还涉及用于检测和表征核酸序列的改进的切割装置。 提供了衍生自各种热稳定生物体的结构特异性核酸酶。 这些结构特异性核酸酶用于切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。
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公开(公告)号：US06372424B1
公开(公告)日：2002-04-16
申请号：US08520946
申请日：1995-08-30
IPC分类号： C12Q170
CPC分类号： C12Q1/6886 , C07H21/00 , C07K14/4746 , C07K14/82 , C12N9/1252 , C12N15/10 , C12N15/113 , C12Q1/682 , C12Q1/6823 , C12Q1/683 , C12Q1/689 , C12Q2537/149 , C12Q2525/301 , C12Q2521/319
摘要： The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5′ nucleases and 3′ exonucleases, are used to detect and identify nucleic acids derived from microorganisms. Methods are provided which allow for the detection and identification of bacterial and viral pathogens in a sample.
摘要翻译： 本发明涉及以位点特异性方式切割核酸切割结构的方法。 酶包括5'核酸酶和3'核酸外切酶，用于检测和鉴定衍生自微生物的核酸。 提供了允许在样品中检测和鉴定细菌和病毒病原体的方法。
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公开(公告)号：US06348314B1
公开(公告)日：2002-02-19
申请号：US09350309
申请日：1999-07-09
IPC分类号： C12Q168
CPC分类号： C12N9/22 , C12Q1/6823 , C12Q1/6827 , C12Q1/683 , Y10S435/81 , Y10S435/822 , C12Q2565/525 , C12Q2561/109 , C12Q2525/301 , C12Q2563/149
摘要： The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译： 本发明涉及核酸序列的检测和表征以及核酸序列的变化的方法。 本发明还涉及在靶序列上形成核酸切割结构并以位点特异性方式切割核酸切割结构的方法。 使用各种酶的结构特异性核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。
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