公开(公告)号：US5290419A

公开(公告)日：1994-03-01

申请号：US45135

申请日：1993-04-12

申请人： Hideki Kambara ,  Takashi Anazawa ,  Keiichi Nagai ,  Hiroaki Machida ,  Hisanori Nasu

发明人： Hideki Kambara ,  Takashi Anazawa ,  Keiichi Nagai ,  Hiroaki Machida ,  Hisanori Nasu

IPC分类号： C12M1/00 ,  G01N21/64 ,  G01N27/447 ,  G01N27/26

CPC分类号： G01N27/44721

摘要： In the multi-color fluorescence detection type electrophoresis apparatus provided with the electrophoresis gel plate, excitation laser source, means to separate the fluorescence images according to each emission wavelength, and the detector of the fluorescence subjected to wavelength selection, two or more laser sources are provided, each of laser lights is irradiated on the sample on a time-sharing basis, and the filter which cuts off the scattered light of each the laser synchronous with the laser beam is installed in front of the wavelength separation means thereby providing simultaneous, quick and real-time analysis of a great number of samples such as DNA and RNA labeled by many types of fluorophores, without overlapping the wavelengths of the excitation light and the fluorescence.

摘要翻译： 在具有电泳凝胶板的多色荧光检测型电泳装置，激发激光源，根据各发光波长分离荧光图像的装置以及进行波长选择的荧光检测器中，两个以上的激光源为 只要分时激光照射在样品上，将与激光同步的每个激光的散射光切断的滤光器安装在波长分离装置的前面，从而提供同时，快速 并对许多类型的荧光团标记的大量样品（如DNA和RNA）进行实时分析，而不会与激发光和荧光的波长重叠。

公开(公告)号：US5938908A

公开(公告)日：1999-08-17

申请号：US847735

申请日：1997-04-22

申请人： Takashi Anazawa ,  Hideki Kambara ,  Satoshi Takahashi ,  Kazumichi Imai ,  Hisanori Nasu

发明人： Takashi Anazawa ,  Hideki Kambara ,  Satoshi Takahashi ,  Kazumichi Imai ,  Hisanori Nasu

IPC分类号： G01N21/64 ,  B01D57/02 ,  G01N27/447 ,  G01N35/00 ,  G01N27/26

CPC分类号： G01N27/44721 ,  G01N27/44782 ,  G01N2035/00237

摘要： A capillary electrophoresis system comprising a plurality of capillaries filled with a migration medium in which samples to which fluorephore labels are added migrate, a light source providing an excitation light exciting the fluorephore labels, a photo detector detecting a fluorescence radiated from the fluorephore labels, and light focusing means placed between the plurality of capillaries to which the excitation light is irradiated. The parts of the plurality of capillaries to which the excitation light is irradiated and the light focusing means are arranged in a plane shape. The excitation light is irradiated through the plurality of capillaries and the light focusing means, the capillaries and light focusing means alternatively placed in the parts where an excitation light is irradiated. The light focusing means consisting of cylindrical rod lenses. The axis of the cylindrical rod lenses is placed substantially parallel with the capillaries, which can detect the fluorescence from samples by irradiating a plurality of capillaries substantially at the same time in a batch without mechanical scanning of a plurality of capillaries or without optical scanning of a light beam, providing a highly sensitive detection of samples.

摘要翻译： 一种毛细管电泳系统，包括填充有迁移介质的多个毛细管，其中添加有荧光标记的样品迁移，提供激发荧光标记的激发光的光源，检测从荧光标记物辐射的荧光的光电检测器，以及 放置在激发光照射到的多个毛细管之间的光聚焦装置。 照射激发光的多个毛细管的部分和光聚焦装置被布置成平面形状。 激发光通过多个毛细管和光聚焦装置照射，毛细管和光聚焦装置交替地放置在照射激发光的部分中。 光聚焦装置由圆柱形棒状透镜组成。 圆柱形棒状透镜的轴线基本上与毛细管平行放置，其可以通过基本上同时照射多个毛细管而检测来自样品的荧光，而不需要对多个毛细管进行机械扫描，或者不进行光学扫描 光束，提供高灵敏度的样品检测。

公开(公告)号：US5935794A

公开(公告)日：1999-08-10

申请号：US969163

申请日：1997-11-12

申请人： Hideki Kambara ,  Kazunori Okano ,  Hisanori Nasu

发明人： Hideki Kambara ,  Kazunori Okano ,  Hisanori Nasu

IPC分类号： C12N15/09 ,  C12N15/00 ,  C12Q1/68 ,  C07H21/02 ,  C12P19/34 ,  G01N33/533

CPC分类号： C12Q1/6869 ,  C12Q1/6837 ,  Y10S435/973 ,  Y10S436/809

摘要： The method of base sequence determination according to the present invention ensures an effective determination of a long DNA base sequence, by providing simultaneous determination of base sequences of two or more positions of the long DNA or base sequences of two or more DNAs, using the DNA probe chip which classifies and retains the DNA oligomers having various sequences, and using fluorophorelabeled primers which have the same sequencies as the oligomers in the chip and are labeled by various fluorophores, then followed by the extension of the determined base length by re-selection of the primers complementary to the sequence thus determined.

摘要翻译： 根据本发明的碱基序列测定方法通过使用DNA来同时测定两个或多个DNA的长DNA或碱基序列的两个或多个位置的碱基序列，从而确保长DNA碱基序列的有效测定 探针芯片，其分类并保留具有各种序列的DNA寡聚体，并使用荧光标记的引物，其具有与芯片中低聚物相同的顺序，并用各种荧光团标记，然后通过重新选择扩增所确定的碱基长度 与由此确定的序列互补的引物。

公开(公告)号：US5741644A

公开(公告)日：1998-04-21

申请号：US465181

申请日：1995-06-05

申请人： Hideki Kambara ,  Kazunori Okano ,  Hisanori Nasu

发明人： Hideki Kambara ,  Kazunori Okano ,  Hisanori Nasu

IPC分类号： C12N15/09 ,  C12N15/00 ,  C12Q1/68 ,  C07H21/04 ,  C12P19/34

CPC分类号： C12Q1/6869 ,  C12Q1/6837 ,  Y10S435/973 ,  Y10S436/809

摘要： The method of base sequence determination according to the present invention ensures an effective determination of a long DNA base sequence, by providing simultaneous determination of base sequences of two or more positions of the long DNA or base sequences of two or more DNAs, using the DNA probe chip which classifies and retains the DNA oligomers having various sequences, and using fluorophorelabeled primers which have the same sequencies as the oligomers in the chip and are labeled by various fluorophores, then followed by the extension of the determined base length by re-selection of the primers complementary to the sequence thus determined.

摘要翻译： 根据本发明的碱基序列测定方法通过使用DNA来同时测定两个或多个DNA的长DNA或碱基序列的两个或多个位置的碱基序列，从而确保长DNA碱基序列的有效测定 探针芯片，其分类并保留具有各种序列的DNA寡聚体，并使用荧光标记的引物，其具有与芯片中低聚物相同的顺序，并用各种荧光团标记，然后通过重新选择扩增所确定的碱基长度 与由此确定的序列互补的引物。

公开(公告)号：US5190632A

公开(公告)日：1993-03-02

申请号：US854872

申请日：1992-03-20

申请人： Hitoshi Fujimiya ,  Hisanori Nasu

发明人： Hitoshi Fujimiya ,  Hisanori Nasu

IPC分类号： G01N21/64 ,  G01N27/447

CPC分类号： G01N27/44721

摘要： The multi-colored electrophoresis pattern reading system compares the patterns of electrophoresis without a warp to be caused upon electrophoresis by reading the patterns of electrophoresis which is carried out concurrently for plural samples and by labelling the samples with fluorescent substances having different fluorescent wavelengths. The patterns of electrophoresis are read by allowing fluorescence to emit from the plural samples. In reading the fluorescent patterns, optical signals are received by the light receiving section and then the fluorescence having a predetermined wavelength is separated by the fluorescent-wavelength separating section from the optical signals with the aid of an optical filter capable of controlling an angle of incidence relative to the optical axis of the optical filter. The optical signals passed through the fluorescent-wavelength separating section is then subjected to optoelectrical conversion into electric signals which in turn are subjected to signal processing, thereby converting the electric signals into a desired form of data representation.

公开(公告)号：US6077670A

公开(公告)日：2000-06-20

申请号：US39278

申请日：1998-03-12

申请人： Kenji Yamamoto ,  Hisanori Nasu ,  Hitoshi Fujimiya ,  Noriko Yurino

发明人： Kenji Yamamoto ,  Hisanori Nasu ,  Hitoshi Fujimiya ,  Noriko Yurino

IPC分类号： G01N33/53 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/533 ,  G01N37/00

CPC分类号： C12Q1/6818

摘要： A method is disclosed which is capable of increasing a S/N ratio without subjecting a sample to any treatment to thereby detect hybridization with high sensitivity. A fragmentarily divided probe is used which is prepared by dividing a single probe 12 into a plurality of fragmentary probes 12a, 12b, and labeling the fragmentary probes with different fluorescent materials 14a, 14b, respectively. When the probe is hybridized with a sample 11, the fragmentary probes 12a,12b reconstitute the single probe. An intermittent light beam is used as excitation light 19. The fluorescent material 14a is excited therewith, and fluorescence 18 emitted from the fluorescent material 14b through multistep excitation is detected while irradiation with the excitation light is intermitted.

摘要翻译： 公开了能够提高S / N比而不对样品进行任何处理从而以高灵敏度检测杂交的方法。 使用通过将单个探针12分成多个部分探针12a，12b并分别用不同的荧光材料14a，14b标记碎片探针来制备的片断分离的探针。 当探针与样品11杂交时，片段探针12a，12b重构单个探针。 使用间歇光束作为激发光19.荧光材料14a被激发，并且通过多脉冲激发从荧光材料14b发射的荧光18被检测，同时激发光的照射被中断。

公开(公告)号：US6071702A

公开(公告)日：2000-06-06

申请号：US364752

申请日：1999-07-30

申请人： Kenji Yamamoto ,  Noriko Yurino ,  Hisanori Nasu

发明人： Kenji Yamamoto ,  Noriko Yurino ,  Hisanori Nasu

IPC分类号： C12Q1/00 ,  B01J19/00 ,  B01L3/00 ,  C12M1/00 ,  C12Q1/68 ,  C40B40/06 ,  C40B60/14 ,  G01N37/00 ,  C07H21/02 ,  C07H21/04 ,  C12P19/34 ,  C12Q1/70

CPC分类号： C12Q1/6834 ,  B01J19/0046 ,  B01L3/5085 ,  B01J2219/00317 ,  B01J2219/00319 ,  B01J2219/00527 ,  B01J2219/00531 ,  B01J2219/00536 ,  B01J2219/00538 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00664 ,  B01J2219/00722 ,  B01L2300/0893 ,  C40B40/06 ,  C40B60/14

摘要： A probe-bearing element according to the present invention includes a sheet-like body having a plurality of through holes which each bears a biopolymer probe. The sheet-like body may have a thickness of 100 .mu.m to 2 mm, and may be made of glass, acrylic resin, metal or plastic. Preferably, the size of the through hole is about 10-100 .mu.m in diameter considering the number of samples relative to the size of the element, amounts of probes required for hybridization and the detection sensitivity. The plurality of through holes are preferably arranged in concentric circles or in a spiral.

摘要翻译： 根据本发明的探针承载元件包括具有多个通孔的片状体，其各自承载生物聚合物探针。 片状体的厚度可以为100μm〜2mm，并且可以由玻璃，丙烯酸树脂，金属或塑料制成。 考虑到相对于元件尺寸的样品数量，杂交所需的探针量和检测灵敏度，优选地，通孔的直径大约为10-100μm。 多个通孔优选地以同心圆或螺旋形布置。

公开(公告)号：US5213673A

公开(公告)日：1993-05-25

申请号：US800847

申请日：1991-11-29

申请人： Hitoshi Fujimiya ,  Hisanori Nasu

发明人： Hitoshi Fujimiya ,  Hisanori Nasu

IPC分类号： G01N21/17 ,  G01N27/447

CPC分类号： G01N27/44721

摘要： A multi-colored electrophoresis pattern reading apparatus is capable of labelling each of plural samples separately with each of plural fluorescent substances having different fluorescence wavelengths, by electrophoresing the plural samples to develop a pattern, exciting the fluorescent substances labelled on the samples to emit fluorescence, and reading a fluorescent pattern emitting the fluorescence. The apparatus contains a source for the light for exciting the fluorescent substances; a light scanning mechanism for scanning the light to irradiate a gel in the direction of its thickness with the light; a light receiving section for receiving fluorescence resulting from the light source separated from the scattered light resulting from a reading surface on the basis of a spatial position relationship of a light receiving path by setting a light receiving surface in a direction different from an optical axis of the irradiating light; an optical filter section for separating the resulting optical signals section into plural wavelengths; an optoelectric conversion section for converting optical signals into electrical signals; and an amplifier for amplifying the electrical signals by an integrating operation corresponding to scanning of the irradiating light, and for generating electrical signals indicative of fluorescence from the pattern one after another.

公开(公告)号：US4706192A

公开(公告)日：1987-11-10

申请号：US727762

申请日：1985-04-26

申请人： Hisanori Nasu ,  Satoru Suzuki ,  Hiroaki Yoshida ,  Tomoichi Fukuda ,  Eiichi Soeda

发明人： Hisanori Nasu ,  Satoru Suzuki ,  Hiroaki Yoshida ,  Tomoichi Fukuda ,  Eiichi Soeda

IPC分类号： G06F3/16 ,  G06F15/42

CPC分类号： G06F3/16

摘要： An input apparatus for entering base sequence information of the gene, comprises a computer for analyzing the base sequence of the gene, an input apparatus for entering base sequence information of the gene to the computer which successively stores the base sequence information entered by the input apparatus and produces the stored base sequence information, and a speech sounding apparatus supplied with the base sequence information produced from the computer for sounding speech corresponding to the base sequence information produced from the computer.

摘要翻译： 一种用于输入基因的碱基序列信息的输入装置，包括用于分析基因的碱基序列的计算机，用于将基因的碱基序列信息输入到计算机的输入装置，其依次存储由输入装置输入的碱基序列信息 并产生所存储的基本序列信息，以及语音探测装置，其被提供有从计算机产生的用于从计算机产生的基本序列信息对应的语音发出的基本序列信息。

公开(公告)号：US07294462B2

公开(公告)日：2007-11-13

申请号：US10802055

申请日：2004-03-17

申请人： Hisanori Nasu ,  Hiroaki Ono ,  Akito Mugita ,  Ichiro Nakayama ,  Takanori Kobayashi ,  Tetsuji Masaoka ,  Satoru Kuhara ,  Yoshizumi Ishino

发明人： Hisanori Nasu ,  Hiroaki Ono ,  Akito Mugita ,  Ichiro Nakayama ,  Takanori Kobayashi ,  Tetsuji Masaoka ,  Satoru Kuhara ,  Yoshizumi Ishino

IPC分类号： C12Q1/68 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12Q1/6837 ,  C12Q2537/125

摘要： There is provided a method for the detection of a base sequence of interest when amount of a sample DNA or RNA is little and plural base sequences of interest to be detected are present in the sample DNA or RNA.The Problem is solved by a method for the detection of an base sequence of interest in a sample DNA or RNA comprising the steps of (1) contacting a sample DNA or RNA to a probe DNAs or RNAs in an aqueous solution to form a hybridization complex; (2) isolating the hybridization complex; (3) dissociating the complex to recover the probe DNAs or RNAs; and (4) identifying the said probe DNAs or RNAs to detect an base sequence of interest in the sample DNA or RNA.

摘要翻译： 提供了当样品DNA或RNA的量少的情况下检测到目标碱基序列的方法，并且待检测的多个碱基序列存在于样品DNA或RNA中。 该问题通过检测样品DNA或RNA中感兴趣的碱基序列的方法来解决，所述方法包括以下步骤：（1）使样品DNA或RNA与水溶液中的探针DNA或RNA接触以形成杂交复合物 ; （2）分离杂交复合体; （3）解离复合物以回收探针DNA或RNA; 和（4）鉴定所述探针DNA或RNA以检测样品DNA或RNA中的目标碱基序列。