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29 条记录 (耗时 0.03 秒)

    COMPOSITION FOR INDUCING PLURIPOTENT STEM CELL, AND USE THEREOF
    1.
    发明申请
    COMPOSITION FOR INDUCING PLURIPOTENT STEM CELL, AND USE THEREOF 有权
    用于诱导大脑干细胞的组合物及其用途

    公开(公告)号：US20130210150A1

    公开(公告)日：2013-08-15

    申请号：US13819235

    申请日：2011-08-30

    申请人： Hiroshi Ban ,  Yasuji Ueda ,  Noemi Fusaki ,  Koichi Saeki ,  Mamoru Hasegawa

    发明人： Hiroshi Ban ,  Yasuji Ueda ,  Noemi Fusaki ,  Koichi Saeki ,  Mamoru Hasegawa

    IPC分类号： C12N15/86

    CPC分类号： C12N15/86 ,  C12N5/0696 ,  C12N15/63 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2506/13 ,  C12N2510/00 ,  C12N2760/18843

    摘要： The present invention provides Sendai virus vectors in which genes that encode reprograming factors for inducing pluripotent stem cells are incorporated in a specific order, compositions comprising these vectors for gene delivery to be used in the induction of pluripotent stem cells, and uses thereof. Incorporation of the KLF gene, OCT gene, and SOX gene in a specific order into a single Sendai virus vector successfully and significantly increased the efficiency of pluripotent stem cell induction. Loading multiple reprogramming factors into a single vector can further increase the induction efficiency of pluripotent stem cells while reducing the number of necessary vectors.

    摘要翻译： 本发明提供仙台病毒载体，其中编码用于诱导多能干细胞的重编程因子的基因以特定顺序并入，包含用于诱导多能干细胞的基因递送的载体的组合物及其用途。 将KLF基因，OCT基因和SOX基因以特定顺序并入单一仙台病毒载体中，并显着提高多能干细胞诱导的效率。 将多个重编程因子加载到单个载体中可以进一步增加多能干细胞的诱导效率，同时减少必需载体的数量。

    Method for producing viral vectors
    7.
    发明申请
    Method for producing viral vectors 审中-公开
    生产病毒载体的方法

    公开(公告)号：US20070141705A1

    公开(公告)日：2007-06-21

    申请号：US10587123

    申请日：2005-01-20

    申请人： Makoto Inoue ,  Hiroshi Ban ,  Akihiro Iida ,  Mamoru Hasegawa

    发明人： Makoto Inoue ,  Hiroshi Ban ,  Akihiro Iida ,  Mamoru Hasegawa

    IPC分类号： C12N5/08 ,  C12N7/01 ,  C12N15/86

    CPC分类号： C12N7/00 ,  C12N2760/18851

    摘要： The present invention provides methods for producing viruses, whose propagation depends on the cleavage of viral protein by a protease, in a manner independent of the protease. The methods of the present invention for producing viruses comprise producing viruses in the presence of a modified viral protein in which the protease cleavage sequence is changed to a cleavage sequence for an alternative protease. Viral vectors can be more efficiently produced by replacing the protease cleavage sequence with a cleavage sequence for a protease expressed endogenously in virus-producing cells. The methods of the present invention enable the production of high titer viruses using a wide variety of cells.

    摘要翻译： 本发明提供了以与蛋白酶无关的方式生产病毒的方法，其传播依赖于蛋白酶对病毒蛋白的切割。 用于产生病毒的本发明的方法包括在修饰的病毒蛋白质的存在下产生病毒，其中将蛋白酶切割序列改变为用于替代性蛋白酶的切割序列。 通过用在病毒产生细胞内源性表达的蛋白酶的切割序列替换蛋白酶切割序列可以更有效地产生病毒载体。 本发明的方法能够使用多种细胞产生高滴度病毒。

    Semiconductor device wherein detection of removal of wiring triggers impairing of normal operation of internal circuit
    10.
    发明授权
    Semiconductor device wherein detection of removal of wiring triggers impairing of normal operation of internal circuit 有权
    其中检测到布线去除的半导体装置会损害内部电路的正常工作

    公开(公告)号：US06545371B1

    公开(公告)日：2003-04-08

    申请号：US09834605

    申请日：2001-04-16

    申请人： Hironori Matsumoto ,  Akihiko Nakano ,  Toshinori Ohmi ,  Eiji Yanagawa ,  Hideyuki Unno ,  Hiroshi Ban ,  Tadao Takeda

    发明人： Hironori Matsumoto ,  Akihiko Nakano ,  Toshinori Ohmi ,  Eiji Yanagawa ,  Hideyuki Unno ,  Hiroshi Ban ,  Tadao Takeda

    IPC分类号： H01L2358

    CPC分类号： H01L23/576 ,  H01L23/573 ,  H01L2924/0002 ,  H01L2924/00

    摘要： A semiconductor device includes, on a protective film laminated on a circuit principal part, (i) a light blocking film provided so as to cover the circuit principal part, (ii) an aluminum oxide film provided so as to completely cover the light blocking film, and (iii) a light-blocking upper wiring provided on the aluminum oxide film. An attempt to exfoliate the light blocking film or the light blocking upper wiring causes the resistance-detection-use upper wiring to break or thin, thereby resulting in an increase in the resistance of the resistance-detection-use wiring. The increase in the resistance is detected by the resistance detecting circuit part, and malfunction or inoperativeness of the circuit principal part is caused in response of detection. By so doing, the circuit principal part can be protected from analysis.

    摘要翻译： 半导体器件包括在层叠在电路主要部分上的保护膜上，（i）设置成覆盖电路主要部分的遮光膜，（ii）设置成完全覆盖遮光膜的氧化铝膜 ，和（iii）设置在氧化铝膜上的遮光上层布线。 剥离遮光膜或遮光上布线的尝试使得电阻检测用上层布线断裂或变薄，从而导致电阻检测用布线的电阻增加。 通过电阻检测电路部分检测电阻的增加，并且响应于检测而引起电路主要部分的故障或不能操作。 通过这样做，可以保护电路主要部分免受分析。