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1.发明授权Method for quantitatively determining homocysteine and a reagent for quantitative determination of homocysteine 有权用于定量测定同型半胱氨酸的方法和用于定量测定同型半胱氨酸的试剂公开(公告)号：US07198890B2
公开(公告)日：2007-04-03
申请号：US10755236
申请日：2004-01-13
CPC分类号： G01N33/6812 , G01N2333/90241
摘要： The present invention provides a method for quantitatively determining homocysteine in a biological specimen containing homocysteine and cysteine by use of an enzyme which is capable of forming hydrogen sulfide both from homocysteine and from cysteine, which comprises (a) reacting the biological specimen with cysteine dioxygenase in the absence of a reducing agent, (b) subsequently reacting the resultant specimen of (a) with a reducing agent and the enzyme which is capable of forming hydrogen sulfide both from homocysteine and from cysteine, and (c) measuring the concentration of the hydrogen sulfide thus obtained to determine the homocysteine concentration in the biological specimen; and a reagent for such a quantitative determination of homocysteine.
摘要翻译： 本发明提供了一种通过使用能够从高半胱氨酸和半胱氨酸形成硫化氢的酶来定量测定含有同型半胱氨酸和半胱氨酸的生物样品中的同型半胱氨酸的方法，其包括（a）使生物样品与半胱氨酸双加氧酶在 不存在还原剂，（b）随后使（a）的所得样品与还原剂和能够从高半胱氨酸和半胱氨酸形成硫化氢的酶反应，和（c）测量氢的浓度 硫化物，以确定生物样品中的同型半胱氨酸浓度; 和用于定量测定同型半胱氨酸的试剂。
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2.发明申请Method for quantitatively determining homocysteine and a reagent for quantitative determination of homocysteine 有权用于定量测定同型半胱氨酸的方法和用于定量测定同型半胱氨酸的试剂公开(公告)号：US20050153385A1
公开(公告)日：2005-07-14
申请号：US10755236
申请日：2004-01-13
CPC分类号： G01N33/6812 , G01N2333/90241
摘要： The present invention provides a method for quantitatively determining homocysteine in a biological specimen containing homocysteine and cysteine by use of an enzyme which is capable of forming hydrogen sulfide both from homocysteine and from cysteine, which comprises (a) reacting the biological specimen with cysteine dioxygenase in the absence of a reducing agent, (b) subsequently reacting the resultant specimen of (a) with a reducing agent and the enzyme which is capable of forming hydrogen sulfide both from homocysteine and from cysteine, and (c) measuring the concentration of the hydrogen sulfide thus obtained to determine the homocysteine concentration in the biological specimen; and a reagent for such a quantitative determination of homocysteine.
摘要翻译： 本发明提供了一种通过使用能够从高半胱氨酸和半胱氨酸形成硫化氢的酶来定量测定含有同型半胱氨酸和半胱氨酸的生物样品中的同型半胱氨酸的方法，其包括（a）使生物样品与半胱氨酸双加氧酶在 不存在还原剂，（b）随后使（a）的所得样品与还原剂和能够从高半胱氨酸和半胱氨酸形成硫化氢的酶反应，和（c）测量氢的浓度 硫化物，以确定生物样品中的同型半胱氨酸浓度; 和用于定量测定同型半胱氨酸的试剂。
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3.发明授权Method of quantitative assay for 1,5-anhydroglucitol and reagent for quantitative assay 失效定量测定1,5-脱水葡萄糖醇和试剂的方法
公开(公告)号：US5871949A
公开(公告)日：1999-02-16
申请号：US980792
申请日：1997-12-01
申请人： Hiroyuki Ebinuma , Koji Ushizawa
发明人： Hiroyuki Ebinuma , Koji Ushizawa
CPC分类号： C12Q1/32
摘要： Using a 1,5-anhydroglucitol dehydrogenase capable of acting on 1,5-anhydroglucitol and directly catalyzing a reducing chromophoric agent in the absence of an electron carrier, the 1,5-anhydroglucitol dehydrogenase is allowed to act on 1,5-anhydroglucitol in the presence of the reducing chromophoric agent preferably after the glucose in the specimen has been changed, or while being changed, into such a structure that it does not react with the 1,5-anhydroglucitol dehydrogenase in the specimen, by the aid of a glucose eliminator. Then, the amount of the resultant reduced colored substance is measured. As the 1,5-anhydroglucitol dehydrogenase, an enzyme produced by a microorganism having the ability to produce the 1,5-anhydroglucitol dehydrogenase is preferably used.
摘要翻译： 使用能够作用于1,5-脱水葡萄糖醇并在不存在电子载体的情况下直接催化还原性发色剂的1,5-脱水葡萄糖醇脱氢酶，使1,5-脱水葡萄糖醇脱氢酶作用于1,5-脱水葡萄糖醇 还原性发色剂的存在优选在样品中的葡萄糖已经被改变或改变之后，使其不与样品中的1,5-脱水葡萄糖醇脱氢酶反应，借助于葡萄糖 消除者 然后，测量所得的还原着色物质的量。 作为1,5-脱水葡萄糖醇脱氢酶，优选使用具有生产1,5-脱水葡萄糖醇脱氢酶能力的微生物产生的酶。
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公开(公告)号：US06541215B1
公开(公告)日：2003-04-01
申请号：US09708454
申请日：2000-11-09
申请人： Hiroyuki Ebinuma , Koji Ushizawa
发明人： Hiroyuki Ebinuma , Koji Ushizawa
IPC分类号： C12Q126
CPC分类号： C12Q1/32 , Y10S435/823 , Y10S435/962
摘要： Disclosed are a method for quantitatively determining mannose, which comprises reacting mannose in a specimen with an enzyme which is capable of oxidizing the mannose by dehydrogenation, in the presence of an electron acceptor, and quantitatively determining a formed reductant of the electron acceptor; and a reagent for the quantitative determination of mannose.
摘要翻译： 本发明公开了一种定量测定甘露糖的方法，其包括使试样中的甘露糖与能够通过脱氢氧化甘露糖的酶在电子受体存在下反应并定量测定形成的电子受体的还原剂; 和用于定量测定甘露糖的试剂。
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5.发明授权Method for determination of hydrogen sulfide or sulfide ion and method for determination of specific substance utilizing said method 有权用于测定硫化氢或硫化物离子的方法和利用所述方法测定特定物质的方法公开(公告)号：US06969613B1
公开(公告)日：2005-11-29
申请号：US09856790
申请日：1999-12-07
申请人： Hiroyuki Ebinuma , Koji Ushizawa
发明人： Hiroyuki Ebinuma , Koji Ushizawa
CPC分类号： C12Q1/527 , G01N31/22 , G01N31/224 , G01N33/0044 , Y10T436/18 , Y10T436/182
摘要： The present invention provides a method for quantitatively determining hydrogen sulfide or sulfide ions conveniently with high sensitivity, which comprises adding to a sample containing hydrogen sulfide or sulfide ions, metal ions or a compound which liberates said metal ions and a metal indicator which reacts with the metal ions and resultingly undergoes color development, wherein the color development is accelerated or inhibited by the hydrogen sulfide or sulfide ions; and measuring the degree of color development of the metal indicator. The present invention further provides a method for quantitatively determining a specific substance, which comprises adding to a sample containing a specific substance, a component which acts on the specific substance so that the specific substance forms hydrogen sulfide or sulfide ions, metal ions or a compound which liberates said metal ions, and a metal indicator which reacts with the metal ions and resultingly undergoes color development, wherein the color development is accelerated or inhibited by the hydrogen sulfide or sulfide ions; and measuring the degree of color development of the metal indicator.
摘要翻译： 本发明提供了一种以高灵敏度方便地定量测定硫化氢或硫化物离子的方法，其包括向含有硫化氢或硫化物离子的金属离子或释放所述金属离子的化合物和与 金属离子发生显色，其中显色被硫化氢或硫化物离子加速或抑制; 并测量金属指示器的显色程度。 本发明还提供了一种定量测定特定物质的方法，该方法包括向含有特定物质的样品中加入作用于特定物质的成分，使特定物质形成硫化氢或硫化物离子，金属离子或化合物 释放出所述金属离子的金属指示剂和与金属离子反应并导致发色的金属指示剂，其中显色被硫化氢或硫化物离子加速或抑制; 并测量金属指示器的显色程度。
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公开(公告)号：US20140080158A1
公开(公告)日：2014-03-20
申请号：US14116683
申请日：2012-05-09
申请人： Kohei Takubo , Hiroyuki Ebinuma , Isamu Fukamachi , Hideaki Bujo , Yasushi Saito
发明人： Kohei Takubo , Hiroyuki Ebinuma , Isamu Fukamachi , Hideaki Bujo , Yasushi Saito
IPC分类号： G01N33/543
CPC分类号： G01N33/54393 , G01N33/92
摘要： To provide a method for assaying soluble LR11 in a biological sample, which method realizes a simple and accurate assay of soluble LR11 present in the sample by immunological means without requiring isolation of soluble LR11 from the biological sample (e.g., a serum sample).The method of the invention for immunologically assaying soluble LR11 present in a biological sample, characterized in that the method includes treating the sample with at least one surfactant selected from among one or more sulfobetaine amphoteric surfactants and one or more amidosulfobetaine amphoteric surfactants.
摘要翻译： 为了提供用于测定生物样品中可溶性LR11的方法，该方法通过免疫学手段实现了样品中存在的可溶性LR11的简单且准确的测定，而不需要从生物样品（例如血清样品）分离可溶性LR11。 用于免疫测定存在于生物样品中的可溶性LR11的本发明的方法，其特征在于所述方法包括用选自一种或多种磺基甜菜碱两性表面活性剂和一种或多种氨基磺基甜菜碱两性表面活性剂中的至少一种表面活性剂处理样品。
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7.发明授权公开(公告)号：US07781171B2
公开(公告)日：2010-08-24
申请号：US10575711
申请日：2004-10-15
申请人： Hiroyuki Ebinuma , Hirokazu Yago , Yuka Akimoto , Osamu Miyazaki , Takashi Kadowaki , Toshimasa Yamauchi
发明人： Hiroyuki Ebinuma , Hirokazu Yago , Yuka Akimoto , Osamu Miyazaki , Takashi Kadowaki , Toshimasa Yamauchi
IPC分类号： G01N33/53
CPC分类号： G01N33/74 , G01N2333/72 , Y10T436/25
摘要： The invention provides a method of pretreating a sample for conveniently, quickly and accurately measuring the total amount of adiponectin present in a biological sample contaminated with various adiponectin multimers.The method of measuring an sample for immunologically assaying the total amount of adiponectin present in the sample comprises reacting, with an adiponectin-containing sample, at least one of a reducing agent, an acid or a salt thereof, a surfactant, and a protease.
摘要翻译： 本发明提供一种方法，用于方便，快速和准确地测量存在于受各种脂联素多聚体污染的生物样品中的脂连蛋白的总量的样品。 用于免疫测定样品中存在的脂联素总量的样品的测定方法包括使含有脂联素的样品与还原剂，其酸或其盐，表面活性剂和蛋白酶中的至少一种反应。
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8.发明授权公开(公告)号：US07244620B2
公开(公告)日：2007-07-17
申请号：US10743741
申请日：2003-12-24
CPC分类号： G01N31/22 , Y10T436/18 , Y10T436/182
摘要： The present invention provides a method for quantitatively determining a reducing substance, which comprises reacting a reducing substance in a test specimen with iron (III) ions, reacting iron (II) ions formed by reduction of the iron (III) ions or residual iron (III) ions with a metal indicator which is capable of reacting specifically with the iron (II) ions or the residual iron (III) ions to undergo color development, and carrying out quantitative determination by measuring the degree of color development, wherein a chelating agent which is specific to copper ions is added to the test specimen before the reaction of the reducing substance with the iron (III) ions; and a reagent used for it.
摘要翻译： 本发明提供了一种定量测定还原物质的方法，该方法包括使试样中的还原物质与铁（III）离子反应，通过还原铁（III）离子或残余铁形成的铁（II） III）离子与能够与铁（II）离子或残留铁（III）离子特异性反应的金属指示剂进行显色，并通过测量显色程度进行定量测定，其中螯合剂 在还原物质与铁（III）离子反应之前，将铜离子特异性添加到试样中; 和用于它的试剂。
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公开(公告)号：US20070054344A1
公开(公告)日：2007-03-08
申请号：US10531305
申请日：2003-10-23
申请人： Hiroyuki Ebinuma
发明人： Hiroyuki Ebinuma
CPC分类号： C07K14/805 , C12N9/0022 , C12N9/2402 , C12P21/00 , C12P21/005 , C12Q1/26 , C12Q1/34 , G01N33/68 , G01N33/725 , G01N2400/00
摘要： The present invention is directed to a defructosylation enzyme originating from a plant, a method of defructosylating a fructosylated peptide or protein through use of the enzyme, and a method of measuring a fructosylated peptide or protein.
摘要翻译： 本发明涉及来自植物的脱甲硅烷基化酶，通过使用酶使果糖基化肽或蛋白质去结构化的方法，以及测量果糖基化肽或蛋白质的方法。
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公开(公告)号：US09541550B2
公开(公告)日：2017-01-10
申请号：US14116683
申请日：2012-05-09
申请人： Kohei Takubo , Hiroyuki Ebinuma , Isamu Fukamachi , Hideaki Bujo , Yasushi Saito
发明人： Kohei Takubo , Hiroyuki Ebinuma , Isamu Fukamachi , Hideaki Bujo , Yasushi Saito
IPC分类号： G01N33/543 , G01N33/92
CPC分类号： G01N33/54393 , G01N33/92
摘要： To provide a method for assaying soluble LR11 in a biological sample, which method realizes a simple and accurate assay of soluble LR11 present in the sample by immunological means without requiring isolation of soluble LR11 from the biological sample (e.g., a serum sample).The method of the invention for immunologically assaying soluble LR11 present in a biological sample, characterized in that the method includes treating the sample with at least one surfactant selected from among one or more sulfobetaine amphoteric surfactants and one or more amidosulfobetaine amphoteric surfactants.
摘要翻译： 用于免疫测定存在于生物样品中的可溶性LR11的本发明的方法，其特征在于所述方法包括用选自一种或多种磺基甜菜碱两性表面活性剂和一种或多种氨基磺基甜菜碱两性表面活性剂中的至少一种表面活性剂处理样品。
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