摘要:
A sensor-integrated wheel support bearing assembly includes an outer member (1) having a plurality of raceway surfaces (4), formed in an inner peripheral surface thereof, and also having a vehicle body fitting flange (1a) formed in an outer periphery thereof, and an inner member (2) having raceway surfaces (5) defined therein in alignment with the raceway surfaces (4) in the outer member (1). A plurality of rows of rolling elements (3) are arranged between the raceway surfaces (4) and (5) to enable a vehicle wheel (18) to be rotatably supported relative to a vehicle body structure. A load sensor (9) for detecting the load acting on the flange (1a) is disposed in a bolt insertion hole (21) provided in the vehicle body fitting flange (1a).
摘要:
A sensor-integrated wheel support bearing assembly for rotatably supporting a vehicle wheel relative to a vehicle body structure has an outer member having an inner peripheral surface formed with a plurality of raceway surfaces, an inner member having an outer peripheral surface formed with raceway surfaces in alignment with the raceway surfaces in the outer member, a plurality of rows of rolling elements interposed between the raceway surfaces in the outer member and the raceway surfaces in the inner member, respectively, and a load sensor. The load sensor is disposed in one of the outer and inner members, which forms a stationary member. The outer member has an outer periphery formed with a vehicle body fitting flange. The load sensor for detecting a load acting on the vehicle wheel by detection of a load acting on the vehicle body fitting flange is disposed in the vehicle body fitting flange.
摘要:
To avoid an adherence of metallic particles from a knuckle to a magnetic encoder during coupling of a wheel support bearing assembly to the knuckle and/or transportation of the wheel support bearing assembly, a protective cap (18) is provided. The wheel support bearing assembly with which the protective cap (18) can be used is of a type including a magnetic encoder (10) provided in a sealing unit (5). This protective cap (18) includes an annular cover-up portion (18a) for enclosing an annular surface of the magnetic encoder (10) that is exposed bare to the outside, and an engagement wall (18b). The engagement wall (18b) is integral with the annular cover-up portion (18a) and of a cylindrical shape capable of being removably engaged in the wheel support bearing assembly.
摘要:
The present invention provides a method of preparing a plasmid having both the ability of expressing retroviral genes and the ability of effecting the after-translational processing of the encoded products, and the resultant plasmid and the expression products thereof. The method of the present invention is to prepare a plasmid by insertion-linking a cDNA fragment containing at least the protease gene from among retroviral genes with a highly expressing gene or a gene direct expressing vector, thereby causing expression of the retroviral genes, and at the same time, to process said expression product itself with the protease in that expression product, thereby mass-producing three kinds of core protein encoded by vaq gene, and three kinds of enzymes encoded by pol gene, in the form of individual single mature or active proteins. Alternatively, the method of the present invention is to prepare a plasmid in which a cDNA fragment containing HIV nef gene region is inserted into a highly expressing gene and a gene direct expression vector, thereby mass-producing Nef protein which is the expression product of nef gene.
摘要:
An expression vector carrying a gene coding for a phosphate-binding protein has been found to have a strong gene expression. The expression vector can be produced by transforming a bacterium belonging to Enterobacteriaceae with a recombinant vector carrying a DNA fragment containing a gene coding for a phosphate-binding protein to form transformants, selecting the transformants containing the desired recombinant vector from said transformants, and isolating the desired recombinant vector from the selected transformants. The expression vector is useful for producing polypeptides.
摘要:
The present invention provides a method of preparing a plasmid having both the ability of expressing retroviral genes and the ability of effecting the after-translational processing of the encoded products, and the resultant plasmid and the expression products thereof. The method of the present invention is to prepare a plasmid by insertion-linking a cDNA fragment containing at least the protease gene from among-retroviral genes with a highly expressing gene or a gene direct expressing vector, thereby causing expression of the retroviral genes, and at the same time, to process said expression product itself with the protease in that expression product, thereby mass-producing three kinds of core protein encoded by gag gene, and three kinds of enzymes encoded by pol gene, in the form of individual single mature or active proteins. Alternatively, the method of the present invention is to prepare a plasmid in which a cDNA fragment containing HIV nef gene region is inserted into a highly expressing gene and a gene direct expression vector, thereby mass-producing Nef protein which is the expression product of nef gene.
摘要:
Disclosed is a method for producing retroviral proteins which are protease,everse transcriptase and endonuclease. The method is characterized by the consecutive expression and processing of retroviral genes by the stepwise cultivation of hosts transformed with a vector constructed to carry retroviral gene fragments comprising at least a protease gene and one or more of the other genes coding for retroviral proteins. The retroviral proteins of this invention are used as specific reagents for the diagnosis of retroviral disease, e.g., AIDS, malignant tumors and so forth, also may be used as the basis for research and development of antiviral agents and a vaccine against the above infectious diseases, and for genetic engineering.