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    Use of sulfonic derivatives of acylated polyethyleneimine bonded phase silica products
    6.
    发明授权
    Use of sulfonic derivatives of acylated polyethyleneimine bonded phase silica products 失效
    使用酰化聚乙烯亚胺键合相二氧化硅产品的磺酸衍生物

    公开(公告)号：US4721573A

    公开(公告)日：1988-01-26

    申请号：US943954

    申请日：1986-12-22

    申请人： Hugh E. Ramsden ,  David R. Nau

    发明人： Hugh E. Ramsden ,  David R. Nau

    IPC分类号： B01D15/08 ,  B01J20/32

    CPC分类号： B01D15/08 ,  B01J20/103 ,  B01J20/262 ,  B01J20/265 ,  B01J20/28004 ,  B01J20/28047 ,  B01J20/28078 ,  B01J20/286 ,  B01J20/291 ,  B01J20/3092 ,  B01J20/3204 ,  B01J20/3217 ,  B01J20/3219 ,  B01J20/3246 ,  B01J20/3272 ,  B01J20/328 ,  B01D15/362 ,  B01J2220/54 ,  B01J2220/58

    摘要： Sulfonic derivatives of N-acylated covalently bound, non-crosslinked polyethyleneimine bonded phase silicas of the formula: ##STR1## whereinis the backbone of a silica gel or glass, n is an integer such that the polyethyleneiminopropyl silane group has an average molecular weight of from about 400 to about 1800; q is an integer of zero or one; R is selected from the group consisting of hydrogen, --CH.sub.2).sub.x H or --CH.sub.2).sub.m COOH where x is an integer of 1 or 2 and m is an integer of zero or 1; when R is hydrogen or --CH.sub.2).sub.x H then R.sup.1 is --CH.sub.2).sub.p COOH where p is an integer of zero or 1, and when R is --CH.sub.2).sub.m COOH then R.sup.1 is selected from the group consisting of hydrogen or --CH.sub.2).sub.y H where y is a integer of zero or 1, are especially useful as solid phases for the purification and separation of proteins having isoelectric points of below about 5.

    摘要翻译： N-酰化共价结合的非交联聚乙烯亚胺键合相二氧化硅的磺酸衍生物，其结构式为：其中是硅胶或玻璃的主链，n是整数，使得聚乙烯亚胺基丙基硅烷基具有 平均分子量为约400至约1800; q是0或1的整数; R选自氢，-CH 2）x H或-CH 2）m COOH，其中x是1或2的整数，m是0或1的整数; 当R为氢或-CH 2）x H时，R 1为-CH 2）p COOH，其中p为0或1的整数，当R为-CH 2时，mCOOH，则R 1选自氢或-CH 2）y H 其中y为零或1的整数，特别可用作用于纯化和分离等电点低于约5的蛋白质​​的固相。

    N-acylated derivatives of polyethyleneimine bonded phase silica products
    9.
    发明授权
    N-acylated derivatives of polyethyleneimine bonded phase silica products 失效
    聚乙烯亚胺键合相二氧化硅产品的N-酰化衍生物

    公开(公告)号：US5066395A

    公开(公告)日：1991-11-19

    申请号：US492219

    申请日：1990-03-13

    申请人： Hugh E. Ramsden ,  David R. Nau

    发明人： Hugh E. Ramsden ,  David R. Nau

    IPC分类号： B01D15/08 ,  B01J20/32

    CPC分类号： B01D15/361 ,  B01D15/362 ,  B01J20/3204 ,  B01J20/3272 ,  B01J20/328

    摘要： Sulfonic derivatives of N-acylated covalently bound, non-crosslinked polyethyleneimine bonded phase silicas of the formula: ##STR1## wherein ##STR2## is the backbone of a silica gel or glass, n is an integer such that the polyethyleneiminopropyl silane group has an average molecular weight of from about 400 to about 1800; q is an integer of zero or one; R is selected from the group consisting of hydrogen, --CH.sub.2).sub.x H or --CH.sub.2).sub.m COOH where X is an integer of 1 or 2 and m is an integer of zero or 1; when R is hydrogen or --CH.sub.2).sub.x H then R.sup.1 is --CH.sub.2).sub.p COOH where p is an integer of zero or 1, and when R is --CH.sub.2).sub.m COOH then R.sup.1 is selected from the group consisting of hydrogen or --CH.sub.2).sub.y H where y is an integer of zero or 1, are especially useful as solid phases for the purification and separation of proteins having isoelectric points of below about 5.

    摘要翻译： N-酰化共价结合的非交联聚乙烯亚胺键合相二氧化硅的磺酸衍生物，其结构式如下：其中是硅胶或玻璃的主链，n是整数，使得聚乙烯亚胺基丙基 硅烷基的平均分子量为约400至约1800; q是0或1的整数; R选自氢，-CH 2）x H或-CH 2）m COOH，其中X为1或2的整数，m为0或1的整数; 当R是氢或-CH 2）x H时，R 1是-CH 2）p COOH，其中p是0或1的整数，当R是-CH 2时，mCOOH，则R 1选自氢或-CH 2）y H，其中 y为零或1的整数，特别适用于纯化和分离等电点低于约5的蛋白质​​的固相。