公开(公告)号：US12077789B2

公开(公告)日：2024-09-03

申请号：US17829866

申请日：2022-06-01

Applicant: ILLUMINA, INC.

Inventor： Misha Golynskiy ,  Mariko Matsuura ,  Saurabh Nirantar ,  Hamed Tabatabaei Ghomi ,  Seth McDonald ,  Ryan Craig ,  Sergio Peisajovich ,  Kyrie Johnson ,  Kay Klausing ,  Eric Murtfeldt ,  Alexandra Exner

IPC: C12N9/12 ,  C12Q1/6874

CPC classification number: C12N9/1252 ,  C12Q1/6874 ,  C12Y207/07007

Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain low pre-phasing rates when using ambiently stored polymerases, as well as methods and kits using the same.

公开(公告)号：US20240026431A1

公开(公告)日：2024-01-25

申请号：US18473564

申请日：2023-09-25

Applicant: Illumina, Inc.

Inventor： Andrew B. Kennedy ,  Lena Storms ,  Fei Shen ,  Olivia Benice ,  Eric Murtfeldt ,  Kaitlin Pugliese ,  Michael Howard

IPC: C12Q1/6844

CPC classification number: C12Q1/6844 ,  C12Q2600/166 ,  C12Q2600/156

Abstract: Described herein are standards and methods of normalizing amplicon size bias. These standards may comprise unique molecular identifiers. In some embodiments, the standards and methods are for use with next generation sequencing (NGS) assays. Also described herein are methods for quantifying DNA damage in a sample comprising DNA using fluorescence or for determining the presence of DNA damage in a library.

公开(公告)号：US20240317793A1

公开(公告)日：2024-09-26

申请号：US18608556

申请日：2024-03-18

Applicant: Illumina, Inc.

Inventor： Hassan Bohra ,  Xiangyuan Yang ,  Ramesh Neelakandan ,  Yin Nah Teo ,  Abdul Sadeer Abd Salam ,  Eric Murtfeldt

IPC: C07H21/00

CPC classification number: C07H21/00

Abstract: In some examples, a nucleoside triphosphate analogue may include a sugar, a nucleobase coupled to the sugar, a triphosphate group coupled to the sugar, a heteroatom coupled to an alpha phosphate of the triphosphate group, and a first substituent coupled to the heteroatom. The heteroatom may be selected from the group consisting of oxygen, nitrogen, and carbon. The first substituent may include at least one of an alkyl chain or a polymer.

公开(公告)号：US20240141427A1

公开(公告)日：2024-05-02

申请号：US18373620

申请日：2023-09-27

Applicant: ILLUMINA, INC.

Inventor： Misha Golynskiy ,  Rahman Rahman Pour ,  Jiawen Li ,  Ryan Craig ,  Hamed Tabatabaei Ghomi ,  Saurabh Nirantar ,  Hsu Myat Noe ,  Lin Hui Chang ,  Yvonne Devadas ,  Jing Wen Lim ,  Kay Klausing ,  Humberto Rojo ,  Eric Murtfeldt ,  Chris Garcia

IPC: C12Q1/6874 ,  C12N9/12

CPC classification number: C12Q1/6874 ,  C12N9/1252 ,  C12Y207/07007

Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain low error rate, low phasing rate, or increased incorporation rate for a second generation ffN under reduced incorporation times, as well as methods and kits using the same.

公开(公告)号：US20230047225A1

公开(公告)日：2023-02-16

申请号：US17829866

申请日：2022-06-01

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED ,  ILLUMINA SINGAPORE PTE. LTD.

Inventor： Misha Golynskiy ,  Mariko Matsuura ,  Saurabh Nirantar ,  Hamed Tabatabaei Ghomi ,  Seth McDonald ,  Ryan Craig ,  Sergio Peisajovich ,  Kyrie Johnson ,  Kay Klausing ,  Eric Murtfeldt ,  Alexandra Exner

IPC: C12N9/12 ,  C12Q1/6874

Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain low pre-phasing rates when using ambiently stored polymerases, as well as methods and kits using the same.