公开(公告)号：US11634697B2

公开(公告)日：2023-04-25

申请号：US17226393

申请日：2021-04-09

Applicant: Illumina, Inc. ,  Illumina Cambridge Limited ,  Illumina Singapore Pte. Ltd.

Inventor： Kay Klausing ,  Hamed Tabatabaei Ghomi ,  Misha Golynskiy ,  Saurabh Nirantar ,  Seth McDonald ,  Sergio Peisajovich

IPC: C12N9/12

Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.

公开(公告)号：US20210348141A1

公开(公告)日：2021-11-11

申请号：US17226393

申请日：2021-04-09

Applicant: Illumina, Inc. ,  Illumina Cambridge Limited ,  Illumina Singapore Pte. Ltd.

Inventor： Kay Klausing ,  Hamed Tabatabaei Ghomi ,  Misha Golynskiy ,  Saurabh Nirantar ,  Seth McDonald ,  Sergio Peisajovich

IPC: C12N9/12

Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.

公开(公告)号：US20240182881A1

公开(公告)日：2024-06-06

申请号：US18538262

申请日：2023-12-13

Applicant: ILLUMINA, INC.

Inventor： Dewei Joel Toh ,  Leslie Yee Ming Beh ,  Shu Ting Tan ,  Anna Traczyk ,  Saurabh Nirantar ,  Eric Brustad ,  Hamed Tabatabaei Ghomi ,  Zahra Fahmi ,  Lekha Ravichandraprabhu ,  Colin Brown ,  Kayla Busby ,  Stephen Gross ,  Rebekah Karadeema ,  Huy Lam ,  Pascale Mathonet ,  Sarah E. Shultzaberger ,  Kathleen Tzeng ,  Allison Kathleen Yunghans

IPC: C12N9/78

CPC classification number: C12N9/78 ,  C12Y305/04005

Abstract: The present disclosure is concerned with proteins, methods, compositions, and kits for mapping of methylation status of nucleic acids, including 5-methylcytosine and 5-hydroxymethyl cytosine (5 hmC). In one embodiment, proteins are provided that selectively act on certain modified cytosines of target nucleic acids and converts them to thymidine or modified thymidine analogues. In another embodiment, proteins are provided that selectively act on certain modified cytosines of target nucleic acids and converts them to uracil or thymidine and selectively do not act on other certain modified cytosines of target nucleic acids. Also provided are compositions and kits that include one or more of the proteins and methods for using one or more of the proteins.

公开(公告)号：US20240141427A1

公开(公告)日：2024-05-02

申请号：US18373620

申请日：2023-09-27

Applicant: ILLUMINA, INC.

Inventor： Misha Golynskiy ,  Rahman Rahman Pour ,  Jiawen Li ,  Ryan Craig ,  Hamed Tabatabaei Ghomi ,  Saurabh Nirantar ,  Hsu Myat Noe ,  Lin Hui Chang ,  Yvonne Devadas ,  Jing Wen Lim ,  Kay Klausing ,  Humberto Rojo ,  Eric Murtfeldt ,  Chris Garcia

IPC: C12Q1/6874 ,  C12N9/12

CPC classification number: C12Q1/6874 ,  C12N9/1252 ,  C12Y207/07007

Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain low error rate, low phasing rate, or increased incorporation rate for a second generation ffN under reduced incorporation times, as well as methods and kits using the same.

公开(公告)号：US20230212534A1

公开(公告)日：2023-07-06

申请号：US18085674

申请日：2022-12-21

Applicant: ILLUMINA, INC. ,  ILLUMINA SINGAPORE PTE LTD

Inventor： Misha Golynskiy ,  Seth McDonald ,  Saurabh Nirantar ,  Matthew Kellinger ,  Michael Previte ,  Sergio Peisajovich ,  Molly He

IPC: C12N9/12 ,  C12N1/20

CPC classification number: C12N9/1241 ,  C12N1/20

Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.

公开(公告)号：US20230047225A1

公开(公告)日：2023-02-16

申请号：US17829866

申请日：2022-06-01

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED ,  ILLUMINA SINGAPORE PTE. LTD.

Inventor： Misha Golynskiy ,  Mariko Matsuura ,  Saurabh Nirantar ,  Hamed Tabatabaei Ghomi ,  Seth McDonald ,  Ryan Craig ,  Sergio Peisajovich ,  Kyrie Johnson ,  Kay Klausing ,  Eric Murtfeldt ,  Alexandra Exner

IPC: C12N9/12 ,  C12Q1/6874

Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain low pre-phasing rates when using ambiently stored polymerases, as well as methods and kits using the same.

公开(公告)号：US12077789B2

公开(公告)日：2024-09-03

申请号：US17829866

申请日：2022-06-01

Applicant: ILLUMINA, INC.

Inventor： Misha Golynskiy ,  Mariko Matsuura ,  Saurabh Nirantar ,  Hamed Tabatabaei Ghomi ,  Seth McDonald ,  Ryan Craig ,  Sergio Peisajovich ,  Kyrie Johnson ,  Kay Klausing ,  Eric Murtfeldt ,  Alexandra Exner

IPC: C12N9/12 ,  C12Q1/6874

CPC classification number: C12N9/1252 ,  C12Q1/6874 ,  C12Y207/07007

Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain low pre-phasing rates when using ambiently stored polymerases, as well as methods and kits using the same.

公开(公告)号：US20230383342A1

公开(公告)日：2023-11-30

申请号：US18325610

申请日：2023-05-30

Applicant: Illumina Cambridge Limited ,  Illumina Singapore Pte. Ltd. ,  Illumina, Inc. ,  Illumina Software, Inc.

Inventor： Xiaolin Wu ,  Patrick McCauley ,  Madushani Dharmarwardana ,  Saurabh Nirantar ,  Misha Golynskiy ,  Xiangyuan Yang ,  Ramesh Neelakandan ,  Benedict Mackworth ,  Carole Anastasi ,  Aathavan Karunakaran ,  Gery M. Vessere ,  David Bracher

IPC: C12Q1/6869 ,  G01N21/64

CPC classification number: C12Q1/6869 ,  G01N2021/6439 ,  G01N2021/6419 ,  G01N21/6428

Abstract: Embodiments of the present disclosure relate to kits, compositions, and methods for nucleic acid sequencing, for example, two-channel nucleic acid sequencing by synthesis using blue and green light excitation. In particular, unlabeled nucleotides for incorporation may be used in conjunction with affinity reagents containing detectable labels excitable by blue and/or green lights, for specific binding to each type of nucleotides incorporated.

公开(公告)号：US11104888B2

公开(公告)日：2021-08-31

申请号：US16670876

申请日：2019-10-31

Applicant: Illumina, Inc. ,  Illumina Singapore Pte. Ltd.

Inventor： Misha Golynskiy ,  Seth McDonald ,  Saurabh Nirantar ,  Matthew Kellinger ,  Michael Previte ,  Sergio Peisajovich ,  Molly He

IPC: C12N9/12

Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.

公开(公告)号：US11001816B2

公开(公告)日：2021-05-11

申请号：US16703569

申请日：2019-12-04

Applicant: Illumina, Inc. ,  Illumina Cambridge Limited ,  Illumina Singapore Pte. Ltd.

Inventor： Kay Klausing ,  Hamed Tabatabaei Ghomi ,  Misha Golynskiy ,  Saurabh Nirantar ,  Seth McDonald ,  Sergio Peisajovich

IPC: C12N9/12

Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.