公开(公告)号：US20230398503A1

公开(公告)日：2023-12-14

申请号：US18332593

申请日：2023-06-09

Applicant: ILLUMINA SINGAPORE PTE. LTD. ,  ILLUMINA, INC.

Inventor： Rajesh Kumar Sharma ,  Gerald Kreindl ,  Anthony Flannery

IPC: B01D69/12 ,  B01D71/02 ,  B01D67/00 ,  B01D61/42

CPC classification number: B01D69/12 ,  B01D71/022 ,  B01D67/0074 ,  B01D61/42 ,  B01D2325/0283 ,  B01D2325/04

Abstract: A nanopore sensing system includes a cis well, a trans well, and a metal based membrane positioned between the cis and trans wells so that a channel defined in the metal based membrane fluidically connects the cis and trans wells. The metal based membrane has a thickness ranging from about 1 nm to about 3 nm and is selected from the group consisting of a metal oxide, a metal sulfide, a metal nitride, a metal phosphide, a metal arsenide, a metal antimonide, a metal selenide, and a metal telluride.

公开(公告)号：US20210276241A1

公开(公告)日：2021-09-09

申请号：US17250276

申请日：2020-01-15

Applicant: Illumina, Inc.

Inventor： Paul M. Crivelli ,  Cyril M. L. Delattre ,  Gerald Kreindl ,  Wesley A. Cox-Muranami

IPC: B29C51/10 ,  B29C69/02 ,  B29C51/14 ,  B29C51/08 ,  B81B1/00 ,  B81C1/00 ,  B81C99/00 ,  B01L3/00

Abstract: Laminated microfluidic structures and methods for manufacturing the same are provided. In some instances, a laminated microfluidic structure is provided which includes a distended region having a sipper port at the bottom and an internal channel that fluidically connects the sipper port to a location outside of the distended region. Thermoforming and/or injection molding techniques for manufacturing such laminated microfluidic structures are provided. In other instances, a laminated microfluidic structure may be co-molded with a polymeric material to produce an integrated laminated microfluidic structure and housing.

公开(公告)号：US20230031325A1

公开(公告)日：2023-02-02

申请号：US17790058

申请日：2020-12-18

Applicant: ILLUMINA, INC.

Inventor： Paul Crivelli ,  Cyril Delattre ,  Gerald Kreindl ,  Wesley A. Cox-Muranami ,  Bryan Crane

IPC: B01L3/00

Abstract: Actuation systems and methods for use with flow cells. In accordance with an implementation, a method includes moving, using an actuator disposed within a manifold assembly, a membrane portion of a membrane of the manifold assembly away from a valve seat to enable fluidic flow from a reagent fluidic line to a common fluidic line. The membrane portion and the valve seat forming a membrane valve. The reagent fluidic line being fluidically coupled to a reagent reservoir. The common fluidic line being fluidically coupled to a flow cell. The common fluidic line has a common central axis and the reagent fluidic line has a reagent central axis that is non-collinear with the common central axis. The method includes urging the membrane portion against the valve seat to prevent fluidic flow from the reagent fluidic line to the common fluidic line.

公开(公告)号：US20220250066A1

公开(公告)日：2022-08-11

申请号：US17727622

申请日：2022-04-22

Applicant: ILLUMINA, Inc.

Inventor： Maxwell Zimmerley ,  LiangLiang Qiang ,  M. Shane Bowen ,  Steven H. Modiano ,  Dajun Yuan ,  Randall Smith ,  Arthur J. Pitera ,  Hai Quang Tran ,  Gerald Kreindl

IPC: B01L3/00

Abstract: An interposer for a flow cell comprises a base layer having a first surface and a second surface opposite the first surface. The base layer comprises black polyethylene terephthalate (PET). A first adhesive layer is disposed on the first surface of the base layer. The first adhesive layer comprises methyl acrylic adhesive. A second adhesive layer is disposed on the second surface of the base layer. The second adhesive layer comprises methyl acrylic adhesive. A plurality of microfluidic channels extends through each of the base layer, the first adhesive layer, and the second adhesive layer.

公开(公告)号：US12296333B2

公开(公告)日：2025-05-13

申请号：US18003284

申请日：2022-02-18

Applicant: Illumina, Inc.

Inventor： Jonathan Ziebarth ,  Jon Aday ,  Paul Crivelli ,  Gerald Kreindl ,  Amit Sharma

IPC: B29C45/14 ,  B01L3/00 ,  B29C45/00

Abstract: Provided herein include various examples of a method for manufacturing aspects of flow cell. The method may include performing chemical processes on a surface of the patterned wafer to prepare the surface of the patterned, singulating the wafer into individual dies, orienting each die on a temporary substrate, where the orienting creates spaces between each individual die, and molding a material over the spaces to create a hybrid wafer comprised of glass and molded material. The method may also include bonding two of the hybrid wafers together, forming a bonded wafer stack.

公开(公告)号：US20240390896A1

公开(公告)日：2024-11-28

申请号：US18790272

申请日：2024-07-31

Applicant: ILLUMINA, Inc.

Inventor： Maxwell Zimmerley ,  LiangLiang Qiang ,  M. Shane Bowen ,  Steven H. Modiano ,  Dajun Yuan ,  Randall Smith ,  Arthur J. Pitera ,  Hai Quang Tran ,  Gerald Kreindl

IPC: B01L3/00

Abstract: An interposer for a flow cell comprises a base layer having a first surface and a second surface opposite the first surface. The base layer comprises black polyethylene terephthalate (PET). A first adhesive layer is disposed on the first surface of the base layer. The first adhesive layer comprises methyl acrylic adhesive. A second adhesive layer is disposed on the second surface of the base layer. The second adhesive layer comprises methyl acrylic adhesive. A plurality of microfluidic channels extends through each of the base layer, the first adhesive layer, and the second adhesive layer.

公开(公告)号：US20200009556A1

公开(公告)日：2020-01-09

申请号：US16457667

申请日：2019-06-28

Applicant: ILLUMINA, Inc.

Inventor： Maxwell Zimmerley ,  LiangLiang Qiang ,  M. Shane Bowen ,  Steven H. Modiano ,  Dajun Yuan ,  Randall Smith ,  Arthur J. Pitera ,  Hai Quang Tran ,  Gerald Kreindl

IPC: B01L3/00

Abstract: An interposer for a flow cell comprises a base layer having a first surface and a second surface opposite the first surface. The base layer comprises black polyethylene terephthalate (PET). A first adhesive layer is disposed on the first surface of the base layer. The first adhesive layer comprises methyl acrylic adhesive. A second adhesive layer is disposed on the second surface of the base layer. The second adhesive layer comprises methyl acrylic adhesive. A plurality of microfluidic channels extends through each of the base layer, the first adhesive layer, and the second adhesive layer.

公开(公告)号：US20230249176A1

公开(公告)日：2023-08-10

申请号：US18003284

申请日：2022-02-18

Applicant: Illumina, Inc.

Inventor： Jonathan Ziebarth ,  Jon Aday ,  Paul Crivelli ,  Gerald Kreindl ,  Amit Sharma

IPC: B01L3/00 ,  B29C45/14

CPC classification number: B01L3/502707 ,  B29C45/14778 ,  B01L2300/0896 ,  B29C2045/0094

Abstract: Provided herein include various examples of a method for manufacturing aspects of flow cell. The method may include performing chemical processes on a surface of the patterned wafer to prepare the surface of the patterned, singulating the wafer into individual dies, orienting each die on a temporary substrate, where the orienting creates spaces between each individual die, and molding a material over the spaces to create a hybrid wafer comprised of glass and molded material. The method may also include bonding two of the hybrid wafers together, forming a bonded wafer stack.

公开(公告)号：US20230112203A1

公开(公告)日：2023-04-13

申请号：US17934175

申请日：2022-09-21

Applicant: ILLUMINA, INC. ,  ILLUMINA SINGAPORE PTE. LTD.

Inventor： Boyan Boyanov ,  Craig Ciesla ,  Anthony Flannery ,  Gerald Kreindl ,  Rean Silke Musa ,  Darren Segale

IPC: G01N33/487 ,  C12Q1/6869 ,  G01N27/414 ,  B01L3/00

Abstract: Devices for sequencing biopolymers and methods of using the devices are disclosed. In one example, such a device has a nanopore, a plurality of wells and fluidic tunnels to allow a biopolymer to translocate in the device. In some embodiments, the device may include integrated electronics or micro-electromechanical systems, such as valves, bubble generators/annihilators or pressure pulse generators, to actively control fluidic/ionic/electric flows in the device.

公开(公告)号：US12083514B2

公开(公告)日：2024-09-10

申请号：US17727622

申请日：2022-04-22

Applicant: ILLUMINA, Inc.

Inventor： Maxwell Zimmerley ,  LiangLiang Qiang ,  M. Shane Bowen ,  Steven H. Modiano ,  Dajun Yuan ,  Randall Smith ,  Arthur J. Pitera ,  Hai Quang Tran ,  Gerald Kreindl

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L3/502707 ,  B01L3/502746

Abstract: An interposer for a flow cell comprises a base layer having a first surface and a second surface opposite the first surface. The base layer comprises black polyethylene terephthalate (PET). A first adhesive layer is disposed on the first surface of the base layer. The first adhesive layer comprises methyl acrylic adhesive. A second adhesive layer is disposed on the second surface of the base layer. The second adhesive layer comprises methyl acrylic adhesive. A plurality of microfluidic channels extends through each of the base layer, the first adhesive layer, and the second adhesive layer.